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All Journal Jurnal Akuakultur Indonesia Zuriat Indonesian Journal of Agricultural Science BERITA BIOLOGI LIMNOTEK - Perairan Darat Tropis di Indonesia Indonesian Aquaculture Journal

Rudhy Gustiano

Balai Penelitian dan Pengembangan Budidaya Air Tawar, Jl. Sempur No. 1, Bogor 16154 Telp. (0251) 8313200

Author-ID : 271461

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Education Environmental Science

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KERAGAMAN GENETIK POPULASI IKAN NILA {Oreochromis niloticus) DALAM PROGRAM SELEKSI BERDASARKAN RAPD Arifin, Otong Zenal; Nugroho, Estu; Gustiano, Rudhy
BERITA BIOLOGI Vol 8, No 6 (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Objectives of the study was to discover genetic variability and genetic relationship of paternal half sib population of nile tilapia (Oreochromis niloticus) under Selection Program Scheme at Research Institute for Freshwater Aquaculture, in Bogor, West Java.Four populations from unrelated selected breeders were observed for genetic variability using RAPD. The analysis of amplification from each locus and fragments were used to estimate DNA polymorphisms, heterozygosity, fst and genetic distance.The range of heterozygosity of four examined populations was 0.1760-0.2168 with level polymorphic between 47.66% and 64.86%.The highest heterozygosity and polymorphic was on population 1 and the lowest one was on population 2. Fst test showed significance among the populations. The closest relationship was between population 1 and 4 (0.1978) and the furthest was population 2 and 3 (0.3289).

DERAJAT PENETASAN DAN SINTASAN LARVA IKAN BAUNG (Hemibagrus nemurus) DALAM DUA SISTEM PENETASAN BERBEDA Prakoso, Vitas Atmadi; Subagja, Jojo; Radona, Deni; Kristanto, Anang Hari; Gustiano, Rudhy
LIMNOTEK - Perairan Darat Tropis di Indonesia Vol 25, No 2 (2018)
Publisher : Research Center for Limnology

Show Abstract | Download Original | Original Source | Check in Google Scholar

Salah satu komoditas ikan air tawar di Indonesia yang memiliki prospek untuk budidaya adalah ikan baung (Hemibagrus nemurus). Namun, teknologi budidaya ikan baung masih perlu disempurnakan, khususnya perbenihannya untuk meningkatkan produktivitas budidaya ikan baung. Salah satu teknologi yang dapat dikembangkan adalah melalui teknik penetasan telur. Penelitian ini bertujuan untuk meningkatkan derajat penetasan dan sintasan larva ikan baung melalui dua sistem penetasan yang berbeda. Penelitian dilakukan di Instalasi Riset Plasma Nutfah Perikanan Air Tawar, Cijeruk, Bogor pada bulan Agustus 2017. Telur diperoleh dari induk ikan baung generasi kedua hasil domestikasi (Bobot induk betina: 443,3 ± 70,9 g; Bobot induk jantan: 486,7 ± 83,3 g). Metode penetasan yang diuji yaitu sistem baki (tray) dan corong (funnel) dengan masing-masing sistem penetasan terdiri dari empat ulangan. Parameter yang diamati adalah waktu penetasan, derajat penetasan dan sintasan larva selama dua hari. Pengukuran kualitas air yang diamati meliputi suhu, oksigen terlarut, dan pH. Hasil penelitian menunjukkan bahwa pada kisaran suhu yang sama, larva menetas pertama kali saat 34 jam setelah pembuahan pada sistem corong. Sementara itu pada sistem baki, larva pertama kali menetas saat 36 jam setelah pembuahan. Derajat penetasan pada sistem corong (65,79 ± 5,49 %) lebih tinggi dibandingkan pada sistem baki (30,60 ± 1,91 %) (P<0,05). Sintasan larva ikan baung selama dua hari tidak berbeda nyata antar sistem penetasan. Berdasarkan hasil penelitian ini, dapat disimpulkan bahwa sistem penetasan pada corong menghasilkan waktu penetasan telur dua jam lebih cepat dan derajat penetasan yang lebih tinggi dibandingkan sistem baki.

HUBUNGAN PANJANG DAN BOBOT IKAN NILA LOKAL, BEST F5 DAN F6 DI PANGKEP, SULAWESI SELATAN PADA UMUR 60 HARI PEMELIHARAAN Kusmini, Irin Iriana; Gustiano, Rudhy; Putri, Fera Permata
BERITA BIOLOGI Vol 13, No 2 (2014)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v13i2.685

Best tilapia is breed fish developed by Research Center and Development of Freshwater Aquaculture (BPPBAT) Bogor that has been launched since 2008. Best tilapia is the third generation of gift tilapia. In order to obtain a better growth performance, BPPBAT continued the process of breeding, and to date we have obtained the sixth generations of Best tilapia. This study was aimed to determine growth of tilapia through observation of the relationship between the length and weight of local tilapia, F5 and F6 BEST which were maintained in brackish waters in the Pangkep area, South Sulawesi. Fish samples were taken at random, 30 fishes were taken from each population for length and weight measurement. Calculation of the length-weight of fish in the Pangkep area obtained regression coefficient (b) for local fish 2.4361; BEST F5 3.1077 and F6 3.0522. These values indicated that the local tilapia was negatively allometric while BEST was positively allometric, with local fish condition factor ranged between 0.471-1.334, F5; 0.468-1.479 and 0.498-1.534 F6 respectively. This results has indicating the normal condition of the fish. The length-weight relationship of fish had a value of determinant (R 2) ranged from 0.7829-0.9182, and indicated fish variations influenced by other variables was rather small and has suggested close relationship between length and weight.

A STUDY ON THE GROWTH AND PHYSIOLOGICAL RESPONSE OF JUVENILE TINFOIL BARB Barbonymus schwanenfeldii (Bleeker, 1854) UNDER THE INFLUENCE OF pH CHANGES Oktavia, Nia; Prakoso, Vitas Atmadi; Kamal, Mohammad Mukhlis; Arifin, Otong Zenal; Subagja, Jojo; Kurniawan, Kurniawan; Iswantari, Aliati; Kusmini, Irin Iriana; Irawan, Deni; Cahyanti, Wahyulia; Radona, Deni; Putri, Fera Permata; Bosman, Ofan; Wibowo, Arif; Kristanto, Anang Hari; Gustiano, Rudhy
Indonesian Aquaculture Journal Vol 19, No 1 (2024): (June, 2024)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.19.1.2024.35-44

Tinfoil barb Barbonymus schwanenfeldii is recognized as one of the potential aquaculture commodities in Indonesia. Nonetheless, lack of data on their environmental tolerance causing this issue to become essential to be investigated. The present study was carried out to assess the pH influence on growth and physiological response of juvenile tinfoil barb. Juvenile tinfoil barbs (TL: 5.5 ± 0.8 cm; BW: 2.4 ± 1.0 g) were treated with four pH level treatments (5, 6, 7, and 8) and three replications with the stocking density of 10 fish each aquarium. Fish were kept for 21 days and fed with commercial fish feed. In this study, pH 8 exposure resulted to lower growth of juvenile tinfoil barb compared to pH treatment 5 to 7 (p<0.05). In terms of survival rate, the results show significantly lower value at pH 8 treatment compared to those of pH 6 and 7 treatment (p<0.05), while it is not significantly different with pH 5 treatment (p>0.05). Additionally, this study found the highest value of glucose, cortisol, and aspartate aminotransferase (AST) on pH 8 treatment (p<0.05). On the other hand, pH 8 exposure led to the lowest creatinine, blood urea nitrogen (BUN), and ALP level (p<0.05). In conclusion, the optimal pH for juvenile tinfoil barb rearing ranged around 6-7.

FIRST GENERATION REPRODUCTIVE PERFORMANCE AND SECOND GENERATION LARVAL PRODUCTION ON THE DOMESTICATED TINFOIL BARB, Barbonymus schwanenfeldii (BLEEKER, 1854) Kusmini, Irin Iriana; Cahyanti, Wahyulia; Gustiano, Rudhy; Sundari, Sri; Iskandar, Andri; Radona, Deni; Kurniawan, Kurniawan; Prakoso, Vitas Atmadi; Putri, Fera Permata; Arifin, Otong Zenal; Subagja, Jojo; Kristanto, Anang Hari; Prihadi, Tri Heru; Mulyasari, Mulyasari; Yosmaniar, Yosmaniar; Subaryono, Subaryono
Indonesian Aquaculture Journal Vol 18, No 2 (2023): (December, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.2.2023.97-104

Tinfoil barb, Barbonymus schwanenfeldii (Bleeker, 1854), is a potential freshwater fish for Indonesian aquaculture. Before widely used, the candidate needs to be evaluated. This research aimed to evaluate the reproductive performance of the first generation (G1) and larval performance of the second generation (G2) of domesticated tinfoil barb. A total of 68 female and 24 male broodstocks were selected for the artificial breeding program. The gonadal maturity test was carried out by canulating the eggs every month. Before spawning, GnRHa hormone was injected into the dorsal area (0.5 mL/kg for female and 0.2 mL/kg for male). Fecundity, fertility rate, hatching rate, embryogenesis, and larvae ontogeny were recorded. The results showed that the first matured G1 males of tinfoil barb were at the standard length of 16.01 ± 1.18 cm, while females at 15.79 ± 1.23 cm. The mature broodstock indicated by the gonad maturity stage III and IV confirming higher estradiol concentration (above 400 pg mL-1). The fecundity of two mature broodstock-sized of 217.2 g and 197.3 g were 12,495 and 15,782 eggs, respectively. The spawning season of G1 tinfoil barb was in October and November (rainy seasons). The fertilized eggs latency time was 10 hours 44 minutes at 25°C and hatched after 23 hours 7 minutes. The fertility rate was 96.96 % and the hatching rate was 95.16 %. The survival rate of G2 normal larvae was 100 % at three days of the rearing period. The G2 larvae production in this experiment provides an excellent opportunity for fish diversification both for aquaculture and restocking.

RESILIENCE AND PHYSIOLOGICAL RESPONSES OF THE DOMESTICATED ASIAN REDTAIL CATFISH Hemibagrus nemurus TO HYPOXIA CONDITION Iswantari, Aliati; Kurniawan, Kurniawan; Prakoso, Vitas Atmadi; Radona, Deni; Irawan, Deni; Putri, Fera Permata; Cahyanti, Wahyulia; Arifin, Otong Zenal; Subagja, Jojo; Gustiano, Rudhy; Kusmini, Irin Iriana; Wibowo, Arif; Kristanto, Anang Hari
Indonesian Aquaculture Journal Vol 18, No 1 (2023): (June, 2023)
Publisher : Center for Fisheries Research, Agency for Marine and Fisheries Research and Human Resource

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15578/iaj.18.1.2023.53-60

Hypoxia is one of the critical issues in aquaculture production systems as it can lead to physiological disturbances in cultured fish. This research aimed to evaluate the tolerance level and physiological responses of domesticated Asian redtail catfish Hemibagrus nemurus reared in various hypoxia conditions. A total of 12 fish/treatment were acclimated to gradually decreased dissolved oxygen treatments until fish experienced aquatic surface respiratory (ASR) and loss of equilibrium (LOE). Cortisol, haemoglobin, and glucose levels were detected in the blood plasma to evaluate the stress response of the fish to hypoxia. The result showed that ASR of H. nemurus was identified at 2.17 ± 0.14 ppm of dissolved oxygen (DO) concentration with the percentage of ASR was 77.67 ± 9.53%, while LOE critical of H. nemurus happened at 0.63 ± 0.15 ppm of DO where 55.56 ± 4.81% of the fish experienced LOE. There were significant differences in the values of physiological parameters (blood cortisol, haemoglobin, and glucose) between control and treatments as fish experienced LOE (P<0.05). In the present study, it was found that the Asian redtail catfish is classified as a hypoxia-sensitive fish group. Tehis finding is valuable information for the rearing and growing of the fish to provide an optimal DO concentration for their growth and survival.

The Success of Freshwater Aquaculture Program: Nile Tilapia or “Nila” Culture In Indonesia Gustiano, Rudhy; Arifin, Otong Z; Subagja, Jojo; Kurniawan, Kurniawan; Prihadi, Tri H; Saputra, Adang; Ath-Thar, M H F; Cahyanti, Wahyulia; Prakoso, Vitas A; Radona, Deni; Kusmini, Irin I; Kristanto, Anang H
Zuriat Vol 34, No 2 (2023): September, 2023
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v34i2.50108

This paper discusses the current status of tilapia aquaculture production, historical development based on the production growth, technological advances in genetic improvement and culture system, trade and product development, strategies, and policies in maintaining and improving national production. All data and information used in this manuscript were collected from available publications relating to the past and present status of tilapia cultivation in the country. Nowadays, Indonesia is the second-largest tilapia producer in the world with the contribution of about 25.89% to tilapia global production. In Indonesia, tilapia is the largest production among other cultured species. The annual growth production of tilapia is inccread 11.61% from 2011 to 2018. West Java Provinceis the largest tilapia producer followed by West Sumatra, South Sumatra, and Central Java. In the last six years, most of tilapia production comes from pond culture followed by floating cages, net cages, paddy fields, and pen culture. The success of tilapia culture is much influenced by technological improve in grow-out and genetic improvement of the local varieties. A side of that, trading and global market oriented are also established with various acceptable products. Tilapia aquaculture in Indonesia shows the succsess story of freshwater aquaculture program from nothing before 1990 to be something at present time. To maintain and increase the future production of tilapia, it needs strategies and policies in production and trade. The tilapia program maybe used as a role model for another economic imprortant freshwater species in Indonesia.

Small Tropical Abalone (Haliotis squamata Reeve, 1846) in Indonesia: Current Research Status and Future Prospect Permana, I Gusti Ngurah; Gustiano, Rudhy; Chodriyah, Umi; Ahmad, Riza Zainuddin; Kusmini, Irin Iriana; Lukman, Lukman; Yosmaniar, Yosmaniar; Kadarini, Tutik; Kurniasih, Titin; Murdinah, Murdinah
Zuriat Vol 35, No 2 (2024): September, 2024
Publisher : Breeding Science Society of Indonesia (BSSI) / PERIPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/zuriat.v35i2.56861

Abalone farming is gaining popularity worldwide as demand grows. The importance of abalone farming throughout Indonesia is due in part to expanding demand for both local and foreign abalone, and to a growing awareness of the necessity for sustainable seafood production. This study relies on fourty-six scientific papers published between 2000 and 2023. The distribution and variety, bio-reproduction and breeding, growth, and commerce of small tropical abalones are carefully explored to highlight the current research state, as well as the opportunities and challenges in the existing abalone sector. Finally, all interactions yield findings that can be utilized to guide government policy. To realize its full potential, the Indonesian abalone industry must overcome difficulties such as competition and price fluctuations. Key goals include developing sustainability collection and trading methods, reliably supplying high-quality abalone goods, and expanding their reach through global trade. Addressing these challenges will enable the Indonesian abalone industry to thrive in the next years. The abalone business in Indonesia has a long history, is now growing and active, and presents significant potential. With ongoing R&D investments, the Indonesian abalone sector can meet national and global market demands while being sustainable. Understanding the current situation of abalone research in Indonesia is critical to maintaining the industry's long-term survival.

Recovery Gonad Betina dan Kontrol Produksi Sperma Secara Hormonal pada Ikan Heterobranchus longifilis Subagja, Jojo; Gustiano, Rudhy
Jurnal Akuakultur Indonesia Vol. 4 No. 2 (2005): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Two experiments were conducted in the IRD-Gamet Laboratory, Montpellier, France. The first experiment was to evaluate the recovery of Heterobranchus longifilis females' gonad after induced ovulation, under tropical condition. Eggs diameter was used as indicator of the recovery rate following by induced breeding, hatching rate and abnormality of larvae. The results showed that the recovery was about 28 - 35 days after ovulation. The second experiment was carried out to improve milt production by hormone treatments, volume of intra testicular sperm and the numbers of spermatozoa were observed. The results indicated that the treatmens did not prove any significant differences. Keywords: hormone, gonad, reproduction, Heterobranchus longifilis ABSTRAK Dua kegiatan penelitian dilakukan di Laboratorium IRD-Gamet, Montpellier, Perancis. Penelitian pertama dilakukan untuk mengetahui recovery gonad ikan Heterobranchus longifilis betina setelah ovulasi, di daerah tropis. Diameter telur digunakan sebagai indikator recovery gonad, diukur pada saat sekitar 28-35 hari setelah ovulasi. Penelitian kedua dilakukan untuk meningkatkan produksi sperma dengan memberikan perlakuan hormon. Volume semen dan jumlah spermatozoa dievaluasi. Hasil penelitian menunjukkan perlakuan tidak memberikan hasil yang signifikan. Kata kunci: hormon, gonad, reproduksi, Heterobranchus longifilis

Co-Authors A.A. Ketut Agung Cahyawan W Aliati Iswantari Arif Wibowo Arifin, Otong Z Ariiin, Otong Zenal Ath-Thar, M H F Bosman, Ofan Cahyanti, Wahyulia Chang, Young Jin Chodriyah, Umi Deni Irawan, Deni Deni Radona, Deni Dinar Tri Soelistyowati Estu Nugroho Fauzan, Agung Luthfi Fera Permata Putri, Fera Permata Gleni Hasan Huwoyon, Gleni Hasan Irin Iriana Kusmini, Irin Iriana Iskandar, Andri Iskandariah, Iskandariah Jojo Subagja Kim, Ki Tae Kristanto, Anang H Kristanto, Anang Hari Kristanto, Anang Hari Kurniawan Kurniawan Kusmini, Irin I Laurent Pouyaud LUKMAN, LUKMAN Mahardhika, Prana MH. Fariduddin Ath-thar Min, Byung Hwa Mohammad Mukhlis Kamal Mulyasari, Mulyasari Mulyasari, Mulyasari Murdinah, Murdinah Oktavia, Nia Oktaviani, Tia Otong Zenal Arifin Permana, I Gusti Ngurah Prakoso, Vitas A Prakoso, Vitas Atmadi Prakoso, Vitas Atmadi Prihadi, Tri H Riza Zainuddin Ahmad Ryu, Jun Hyung Saputra, Adang Soelistyowati, Dinar Subaryono Subaryono, Subaryono Sundari, Sri Titin Kurniasih Tri Heru Prihadi Tutik Kadarini Wahyutomo, Wahyutomo Yosmaniar, Yosmaniar

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