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CORRELATION BETWEEN BLOOD METABOLITE AND REPRODUCTIVE PERFORMANCE OF LACTATING HOLSTEIN FRIESIAN CROSSBRED COWS IN SMALLHOLDER FARMERS Widayati, Diah Tri; Paramita, Marcella Arka; Dwiviyanti, Elisa; Suranindyah, Yustina Yuni
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.13428

Abstract

This study examined the correlation between the concentration of blood metabolites (triglyceride, albumin, phosphorous) on the reproductive performances (S/C and PPM) of lactating Holstein Friesian crossbred (HFC) cows. Blood samples were collected through the caudal vein 8 hours after feeding and the plasma was analyzed at the Integrated Research and Testing Laboratory of Universitas Gadjah Mada (LPPT UGM). Samples were stored at 5 C. Triglyceride, albumin, and phosphorous were measured using glucose oxidase-phenol 4-aminoantipyrine (GOD-PAP) method, photometric bromocresol green method, and cypress diagnostic, respectively. Blood metabolites data were analyzed using Pearson correlation model. The results showed that the correlation coefficients of triglycerides, albumin, and phosphorus levels on PPM and S/C were -0.521 and -0.650; -0.447 and -0.612; -0.513 and -0.700, respectively. In conclusion, there are significant negative correlation between triglyceride, albumin and phosphorus levels on the PPM, and very significant negative correlation between triglyceride, albumin and phosphorus levels on the S/C of lactating HFC cows.
EVALUATION OF FRIESIAN HOLSTEIN GRADE COWS FERTILITY BASED THE LEVEL OF MILK UREA Widayati, Diah Tri; Suranindyah, Yustina Yuni; Rahmah, Laelatul; Widyobroto, Budi Prasetyo
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.4929

Abstract

This study was conducted to evaluate fertility grade of Friesian Holstein cows based on milk urea levels. A number of eight cows which fulfill the criteria of not pregnant and in second to sixth lactation, produced milk of 2.5 liters/day, and body weight ranging from 400-450 kg were used in this research. The cows were given fodder that consisted of 30 kg of forage and 10 kg of a mixture of concentrate (pollard, pulp out, and molasses), in the morning and afternoon. Milk and blood samples were taken once a week for five weeks in the morning before fed. Milk urea levels were measured using the urea FS (DiaSys) and blood samples were tested using enzyme linked immuno-sorbent assay (ELISA) with the progesterone hormone kit (DRG, Germany). The variables measured were pregnancy rate, service per conception (S/C), days open, and progesterone levels. Reproduction and milk urea levels data were analyzed by linear regression to determine the relationship between two variables. Progesterone data were analyzed descriptively. The results showed that the average calving interval, days open, and S/C were 607.5170.27 days; 341.25187.65 days; and 2.412.32 days, respectively. The average of milk urea levels was 14.162.55 mg/dL. Regression analysis showed a significant effect (P0.05) and negative correlation between milk urea level and S/C. The average of non-pregnant progesterone hormone level was 12.047.13 ng/mL. In conclusion, the fertility grade of Friesian Holstein cows decreased when the milk urea level at 14.16 mg/dL and non-pregnant progesterone at 12.047.13 ng/mL.
The Influence of Parity on Blood Metabolite Profiles and Reproductive Performance in Saanen-Etawah Crossbred Does Diatmono, Dio Fico Felsidan; Paramita, Stefani Winda; Padmawati, Fransisca Gani; Sitaresmi, Pradita Iustitia; Kumala, Seraphina; Andi, Megawati; Widyobroto, Budi Prasetyo; Suranindyah, Yustina Yuni; Freitas, Joana da Costa; Widayati, Diah Tri
Buletin Peternakan Vol 49, No 4 (2025): BULETIN PETERNAKAN VOL. 49 (4) November 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i4.110480

Abstract

This study aimed to observe the effect of parity on the blood metabolite profiles and reproductive performance of Saanen-Etawah Crossbred does, maintained in smallholder farm. A total of 40 non-pregnant lactating does were divided into two groups: primiparous (n=16) and multiparous (n=24). The blood metabolite profiles, including total protein, glucose, cholesterol, and blood urea nitrogen (BUN), were assessed. Blood samples were collected from the jugular vein during the follicular phase, two hours prior morning feeding. Analysis of blood metabolites was carried out using photometric atomic absorption spectroscopy (AAS)-flame procedure. Furthermore, reproductive performance was observed through observing the characteristics of estrus and the length of the estrus cycle. The results indicated total protein in blood were significantly higher (p<0.05) in multiparous does (7.04±0.87 g/dL) compared to primiparous does (5.47±1.40 g/dL). In contrast, primiparous does exhibited significantly higher (p<0.05) blood cholesterol levels (122.33±30.55 mg/dL) than multiparous does (107.14±13.45 mg/dL). Multiparous does displayed typical estrus characteristics, including vulvar reddening, swelling, and vaginal mucus production, alongside a significantly shorter estrus cycle length. Parity was positively correlated with total protein levels (r=0.577), vulvar reddening (r=0.355), vulvar swelling (r=0.343), and vaginal mucus production (r=0.450), while negatively correlated with cholesterol levels (r=-0.330) and estrus cycle length (r=-0.532). These findings indicate that parity influences total protein and cholesterol levels, as well as several key estrus characteristics. Further investigation into optimized feed regimens and reproductive hormone fluctuations is warranted to enhance the reproductive efficiency of Saanen-Etawah Crossbred does.