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All Journal Ulul Albab: Jurnal Studi Islam Alchemy : Journal of Chemistry El-HARAKAH : Jurnal Budaya Islam Journal of Islamic Pharmacy Science and Technology Indonesia Walisongo Journal of Chemistry Molekul: Jurnal Ilmiah Kimia Jurnal Tumbuhan Obat Indonesia al Kimiya : Jurnal Ilmu Kimia dan Terapan
Elok Kamilah Hayati
Universitas Islam Negeri Maulana Malik Ibrahim Malang
Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Physics Public Health

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IDENTIFIKASI SENYAWA METABOLIT SEKUNDER EKSTRAK n-HEKSANA BATANG KESEMBUKAN (Paederia foetida Linn) Hayati, Elok Kamilah; Muti'ah, Roihatul; Chusna, Ifaridatul
ALCHEMY ALCHEMY (Vol.2, No.3
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.287 KB) | DOI: 10.18860/al.v0i0.2903

Abstract

Tanaman kesembukan (Paaderia foetida Linn) merupakan salah satu tanaman yang sering dimanfaatkan oleh masyarakat sebagai obat, sehingga dapat diketahui bahwa tanaman mempunyai aktivitas biologi, begitu juga dengan bagian batangnya. Tujuan penelitian ini adalah untuk mengetahui kandungan golongan senyawa ekstrak heksanadengan metode Kromatografi Lapis Tipis (KLT).Penelitian ini dilakukan dengan mengekstraksi sampel dengan pelarut n-heksana.Golongan senyawa yang terkandung dalam ekstrak klorofom diidentifikasi dengan fitokimia dengan menggunakan reagen. Pemisahan golongan senyawanya digunakan kromatografi lapis tipis (KLT). Uji fitokimia menunjukkan ekstrak n-heksana mengandung steroid. Pemisahan dengan KLT menggunakan eluen n-heksana-etil asetat (8:2) menghasilkan 4 noda dengan nilai Rf berturut-turut 0,10; 0,35; 0,61; dan 0,68
Anticancer Activity against Breast Cancer Cells T47D and Identification of Its Compound from Extracts and Fractions of Leaves Bamboo Grass (Lophaterum gracile B.) Istiqomah, Alfi; Muti'ah, Roihatul; Hayati, Elok Kamilah
ALCHEMY ALCHEMY (Vol.4, No.1
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.682 KB) | DOI: 10.18860/al.v4i1.3138

Abstract

Bamboo grass (Lophatherum gracile B.) is one of weed plants which frequently used as a medicine because it provides active compounds, for instance steroids and tannins. This research was conducted to determine the anticancer activity of extracts and fractions of leaves bamboo grass in inhibiting the growth of breast cancer cells T47D, and to determine the class of the active compounds contained in extracts and fractions which have the lowest IC50 value from phytochemical test and identification with UV-Vis instruments and FTIR. The extraction of active compound leaves of bamboo grass is done by maceration method using ethanol 80%, hydrolysis and partition with n-hexane and chloroform. Four of the extracts is phytochemical tested and anticancer activity tested towards breast cancer cells T47D with MTT method. The best results of the test activities are performed by the separation of classes of active compounds KLTP and are identified using UV-Vis spectrophotometer and FTIR. The value of IC50 from 80% ethanol extraction is 321,389, from hydrolysis extraction is 481.984, from the fraction of chloroform is 177.852, and from n-hexane fraction is 300.681 µg / mL. The lowest IC50 which have the best cytotoxic is the fraction of chloroform and n-hexane. The results of phytochemical test fractions of chloroform and n-hexane fraction indicates the tannin and steroids. The identification result by using UV-Vis and FTIR shows that the fraction of chloroform and n-hexane fraction is containing tannin, which isproven by maximum wavelength at 331.6 nm and 331.6 nm allegations of -OH, CH, C=C. Moreover, the existence of steroid compounds in fraction chloroform and n-hexane is shown by the absorption at 279.1 nm and 278.0 nm of wavelength with allegations of -OH, CH, C=C, C-O.
PEMISAHAN DAN IDENTIFIKASI EKSTRAK KASAR SESKUITERPEN DAUN BUNGA MATAHARI (Helianyhus annuus L.) DENGAN KROMATOGRAFI LAPIS TIPIS Muti'ah, Roihatul; Hayati, Elok Kamilah; Triastutik, Yani
ALCHEMY ALCHEMY (Vol.2, No.3
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (240.897 KB) | DOI: 10.18860/al.v0i0.2905

Abstract

The purpose of this research wasto separate and identify the leaf crude extracts esquiterpene of Sun flower (Helianthus annuus L.) using thin layer chromatography.Sun flower leaf maceration method performed with the solvent methanol. Then performed liquid extraction with ethyl acetate and n-hexane solvent. Ethyl acetate extract fraction and n-hexane extract fraction furth erphyto chemical test. After being test edphyto chemical with reagents, both extracts was followed by sesquiterpene content identification using thin layer chromatography (TLC)  analytic.Phytochemical test result from ethyl acetate extract fraction was positive terpenoid, sesquiterpene and triterpene, while n-hexane extractfraction positive terpenoid, sesquiterpene andsteroid. All egedsesquiterpene with eluentdichloromethane: ethyl acetate (4,8:0,2) is shown with apurplestain. In the ethyl acetate extract fraction all egedsesquiterpene having Rf values of 0.89; 0.94, and 0.96. While n-hexane extract fraction, the resulting eluental legedsesquiterpene Rf 0.49; 0.8,and 0.99.
IDENTIFIKASI SENYAWA DAN AKTIVITAS ANTIMALARIA IN VIVO EKSTRAK ETIL ASETAT TANAMAN ANTING-ANTING (Acalypha indica L.) Elok Kamilah Hayati; Akyunul Jannah; Rachmawati Ningsih
Molekul Vol 7, No 1 (2012)
Publisher : Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (428.59 KB) | DOI: 10.20884/1.jm.2012.7.1.103

Abstract

Telah dilakukan penelitian identifikasi ekstrak etil asetat dari tanama Anting-anting (Acalypha indica Linn.) sebagai senyawa antimalaria dan aktivitasnya secara in vivo pada sel parasit malaria P. berghei.Penelitian ini meliputi ekstraksi tanaman anting-anting menggunakan metode ekstraksi maserasi selama 24 jam dengan variasi pelarut yaitu etil asetat, diklorometana, dan petroleum eter. Pengadukkan dibantu dengan shaker selama 3 jam. Ekstrak pekat diuji fitokimia didukung Kromatografi Lapis Tipis, Ekstrak pekat etil asetat dilakukan uji antimalaria in vivo terhadap hewan uji terhadap sel parasit P. berghei. Data derajat parasitemia mencit dianalisis menggunakan program SPSS dengan Uji OneWay ANOVA dan dilanjutkan dengan Uji Tukey.Hasil penelitian menunjukkan adanya senyawa aktif tanin, alkaloid dan steroid pada ekstrak etil asetat.Uji aktivitas antimalaria secara in vivo pada hewan coba didapatkan hasil penghambatan ekstrak etilasetat terhadap pertumbuhan Plasmodium berghei pada dosis 0,01 mg/g bb sebesar 87,19%; pada dosis 0,1mg/g bb sebesar 84,9% dan pada dosis 1mg/g bb sebesar 90,74%.
Tumbuhan dan Hewan: Alternatif Pengobatan Warisan Budaya Islam Elok Kamilah Hayati
el Harakah: Jurnal Budaya Islam Vol 9, No 1 (2007): EL HARAKAH
Publisher : UIN Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.49 KB) | DOI: 10.18860/el.v9i1.4662

Abstract

The use of plants extracts for medicinal purposes has been going on for thousands of years. Some of the plants products currently used, either in their natural form or as derivatives, were often used originally for other purposes, such as part of rituals, drugs, and even cosmetics. Nowadays, there is a tendency of “going back to nature” as a source of alternative medicine. There are two medicine types; first, chemistry medicine (synthetic drug) and natural medicine (natural drug). Between both, natural medicine can stay because it is more peaceful and can be accepted by body because it has small side effects and cheaper. Alquran has been explained that Allah was commanded us to make use of animal and plant as drugs, for example: honey from bee, cumin, grape, ruby, pomegranate, ginger, and olive. This proves that Alquran contains real and correct news and information. Therefore, the animals and plants were chosen as alternative medicine. Penggunaan tumbuhan dan ekstraknya untuk pengobatan telah dilakukan selama ribuan tahun. Beberapa produk tumbuhan yang sekarang digunakan, baik itu dalam bentuk asli maupun produk jadi, sering dimanfaatkan pula untuk tujuan lain seperti ritual, pengobatan, dan bahkan kosmetik. Saat ini, ada kecenderungan “kembali pada alam” sebagai sumber pengobatan alternatif. Ada dua tipe pengobatan. Pertama, kimiawi (obat sintetis) dan alami. Di antara keduanya, pengobatan alami masih bertahan karena lebih menenangkan dan dapat diterima oleh tubuh karena memiliki efek yang minim dan lebih murah. Dalam Alquran telah dijelaskan bahwa Allah menganjurkan manusia untuk memanfaatkan hewan dan tumbuhan sebagai obat-obatan, contohnya: madu dari lebah, jinten, anggur, delima, jahe, dan zaitun. Ini membuktikan bahwa Alquran mengandung berita dan informasi yang benar. Maka dari itu, hewan dan tumbuhan dipilih sebagai obat alternatif.
Analisis Sidik Jari Kromatografi Lapis Tipis Tanaman Anting-Anting (Acalypha indica L.) Muhammad Teguh Laksono; Elok Kamilah Hayati
ALCHEMY:Journal of Chemistry Vol 9, No 2 (2021): ALCHEMY JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v9i2.11613

Abstract

Anting-anting plant (Acalypha indica L.) is a plant that the community has used as raw material for herbal medicine. Anting-anting plants contain chemical compounds, so it is difficult to ensure these plants' safety and quality control. The research objective was to validate the method by knowing the stability, specificity, precision, and rigidity of the chromatographic profile of alkaloid compounds in the TLC analysis of the anting-anting extract. Extraction was carried out using ethyl acetate solvent for 20 minutes with a frequency of 42 kHz. The eluent used is cyclohexane: toluene: diethylamine (75:15:10) with Dragendorff reagent. The method of validation was acceptable according to the criteria except for the stability test of the analyte during chromatography. The instability of the analyte is indicated that not all spots form a straight diagonal line. The specificity of the method was carried out by comparing the fingerprint patterns of the leaves of the anting-anting with ekor kucing (Acalypha hibsida Farm.) and teh-tehan plants (Acalypha siamensis). The fingerprint profile showed a yellow stain (Rf = 0.39) on the leaves of the anting-anting, which did not appear in the fingerprint profiles of the two comparison samples. Keywords: anting-anting, fingerprint analysis, thin layer chromatography, method validation Anting-anting (Acalypha indica L.) merupakan tanaman yang telah dimanfaatkan masyarakat sebagai bahan baku obat herbal. Anting-anting mengandung banyak senyawa kimia sehingga sulit menjamin keamanan dan pengendalian mutu dari tanaman tersebut. Penelitian ini dilakukan untuk validasi metode dengan mengetahui kestabilan, spesifitas, presisi, dan ketegaran profil kromatografi senyawa alkaloid pada analisis KLT ekstrak anting-anting. Ekstraksi dilakukan menggunakan pelarut etil asetat selama 20 menit dengan frekuensi 42 kHz. Eluen yang digunakan adalah sikloheksana : toluena : dietilamin (75:15:10) dengan reagen Dragendorff. Validasi metode dapat diterima sesuai kriteria kecuali uji stabilitas analit selama kromatografi. Ketidakstabilan analit ditunjukkan dengan seluruh noda yang dihasilkan tidak membentuk garis diagonal yang lurus. Spesifitas pada metode dilakukan dengan membandingkan pola sidik jari daun anting-anting dengan daun ekor kucing (Acalypha hibsida Farm) dan daun teh-tehan (Acalypha siamensis). Profil sidik jari menunjukkan bahwa terdapat noda berwarna kuning kehijauan (Rf = 0,39) pada daun anting-anting yang tidak muncul pada profil sidik jari kedua sampel pembanding tersebut. Kata kunci: anting-anting, analisis sidik jari, kromatografi lapis tipis, validasi metode
Aktivitas Antimalaria Ekstrak Kasar Etanol dan Fraksi n-Heksana Rumput Bambu (Lophatherum gracile B.) secara in Vitro Ella Wulandari; Dewi Yuliani; Elok Kamilah Hayati; Roihatul Muti'ah
ALCHEMY:Journal of Chemistry Vol 6, No 1 (2018): ALCHEMY: JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (339.341 KB) | DOI: 10.18860/al.v6i1.6766

Abstract

Malaria is a disease caused by infectious parasite Plasmodium falciparum and can be transmitted through mosquito bite. The aim of this research was to study antimalarial activity in vitro on crude ethanol extract and n-hexane fraction of bamboo grass (Lophatherum gracile B.). Extraction was carried out by ethanol 80% solvent and fractionation was conducted by n-hexane. Determination of antimalarial activity was subjected to P. falciparum strain 3D7. According to phytochemical test, crude ethanol extract contained tannin and terpenoid, whilst n-hexane fraction contained tannin and steroid. The capability of crude ethanol extract and n-hexane fraction to inhibit P. falciparum was represented by IC50 value. The value of both samples respectively was 12.49 and 61.49 µg/mL. Identification based on LC-MS (liquid chromatography-mass spectrometry), n-hexane fraction shown the presence of tannin and steroid compounds.  Malaria merupakan penyakit yang disebabkan oleh infeksi parasit Plasmodium falciparum yang dapat ditularkan melalui gigitan nyamuk. Penelitian ini bertujuan untuk mengetahui aktivitas antimalaria secara in vitro pada ekstrak kasar etanol dan fraksi n-heksana rumput bambu (Lophatherum gracile B.). Proses ekstraksi dilakukan dengan pelarut etanol 80% dan fraksinasi dengan n-heksana. Uji aktivitas antimalaria dilakukan pada parasit P. falciparum strain 3D7. Hasil uji fitokimia menunjukkan ekstrak etanol mengandung tanin dan terpenoid, sedangkan fraksi n-heksana mengandung tanin dan steroid. Kemampuan ekstrak etanol dan fraksi n-heksana dalam menghambat parasit P. falciparum menghasilkan nilai IC50 masing-masing sebesar 12,49 dan 61,49 µg/mL. Identifikasi senyawa dengan KC-SM (kromatografi cair-spektrometri massa) pada fraksi n-heksana menunjukkan adanya senyawa tanin dan steroid.
Chemometric Analysis on Fingerprints of Acalypha Indica L Based on the Different Drying Methods Hayati, Elok Kamilah; Anggraini, Maulinda Putri
Walisongo Journal of Chemistry Vol 6, No 2 (2023): Walisongo Journal of Chemistry
Publisher : Department of Chemistry Faculty of Science and Technology Walisongo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/wjc.v6i2.14865

Abstract

The use of an anting-anting plant (Acalypha indica Linn) as a raw material of herbal medicine requires a standardized process in its production. This research aims to determine the fingerprint pattern of active compounds using a thin-layer chromatography method with specific samples that applied various drying methods, such as direct sunlight drying, greenhouse drying, and oven drying at 50°C. The chemometrics data interpretation with the Principal Component Analysis (PCA) method was applied to analyze the obtained fingerprint patterns to identify the differences among the variations. The separation of active compounds in the anting-anting plant using ultrasonic extraction for 20 minutes with ethyl acetate as the solvent. Consecutively, the thin-layer chromatography fingerprint was investigated by applying a subsequent comparison of mobile phase cyclohexane: toluene: diethylamine by 75:15:10. The result from the TLC plate spots was examined to detect the differences among the fingerprints, with the help of ImageJ and Orange software. The fingerprints with various drying methods resulted in the possession of 11 stains of active compounds. The ImageJ software generated chromatograms with Area Under the Curve (AUC) values, further subjected to chemometrics analysis using PCA. The PCA results showed simple grouping patterns for each variation, with a total principal component (PC) of 86.03% (PC1 = 52.78%, PC2 = 33.25%), indicating the success criteria of PCA chemometrics analysis.
Analisis Sidik Jari Kromatografi Lapis Tipis Tanaman Anting-Anting (Acalypha indica L.) Laksono, Muhammad Teguh; Hayati, Elok Kamilah
ALCHEMY:Journal of Chemistry Vol 9, No 2 (2021): ALCHEMY JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v9i2.11613

Abstract

Anting-anting plant (Acalypha indica L.) is a plant that the community has used as raw material for herbal medicine. Anting-anting plants contain chemical compounds, so it is difficult to ensure these plants' safety and quality control. The research objective was to validate the method by knowing the stability, specificity, precision, and rigidity of the chromatographic profile of alkaloid compounds in the TLC analysis of the anting-anting extract. Extraction was carried out using ethyl acetate solvent for 20 minutes with a frequency of 42 kHz. The eluent used is cyclohexane: toluene: diethylamine (75:15:10) with Dragendorff reagent. The method of validation was acceptable according to the criteria except for the stability test of the analyte during chromatography. The instability of the analyte is indicated that not all spots form a straight diagonal line. The specificity of the method was carried out by comparing the fingerprint patterns of the leaves of the anting-anting with ekor kucing (Acalypha hibsida Farm.) and teh-tehan plants (Acalypha siamensis). The fingerprint profile showed a yellow stain (Rf = 0.39) on the leaves of the anting-anting, which did not appear in the fingerprint profiles of the two comparison samples. Keywords: anting-anting, fingerprint analysis, thin layer chromatography, method validation Anting-anting (Acalypha indica L.) merupakan tanaman yang telah dimanfaatkan masyarakat sebagai bahan baku obat herbal. Anting-anting mengandung banyak senyawa kimia sehingga sulit menjamin keamanan dan pengendalian mutu dari tanaman tersebut. Penelitian ini dilakukan untuk validasi metode dengan mengetahui kestabilan, spesifitas, presisi, dan ketegaran profil kromatografi senyawa alkaloid pada analisis KLT ekstrak anting-anting. Ekstraksi dilakukan menggunakan pelarut etil asetat selama 20 menit dengan frekuensi 42 kHz. Eluen yang digunakan adalah sikloheksana : toluena : dietilamin (75:15:10) dengan reagen Dragendorff. Validasi metode dapat diterima sesuai kriteria kecuali uji stabilitas analit selama kromatografi. Ketidakstabilan analit ditunjukkan dengan seluruh noda yang dihasilkan tidak membentuk garis diagonal yang lurus. Spesifitas pada metode dilakukan dengan membandingkan pola sidik jari daun anting-anting dengan daun ekor kucing (Acalypha hibsida Farm) dan daun teh-tehan (Acalypha siamensis). Profil sidik jari menunjukkan bahwa terdapat noda berwarna kuning Untuk menambah keseruan bermain, indohoki77 menawarkan berbagai bonus dan promosi menarik kehijauan (Rf = 0,39) pada daun anting-anting yang tidak muncul pada profil sidik jari kedua sampel pembanding tersebut. Kata kunci: anting-anting, analisis sidik jari, kromatografi lapis tipis, validasi metode
Analisis Sidik Jari Kromatografi Lapis Tipis Secara Kemometrik Daun Tumbuhan Anting-Anting (Acalypa Indica L.) Berdasarkan Lokasi Tumbuh Hidayaty, Alfianita Nuril; Hayati, Elok Kamilah
ALCHEMY:Journal of Chemistry Vol 12, No 1 (2024): ALCHEMY: JOURNAL OF CHEMISTRY
Publisher : Department of Chemistry, Faculty of Science and Technology UIN Maulana Malik Ibrahim Malan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/al.v12i1.22130

Abstract

Anting-anting plant (Acalypha indica L.) is a tropical plant with abundant secondary metabolite content, so its content can be used as traditional medicine. One of the secondary metabolites is influenced by the location of growth in different groups of compounds and levels. Information related to secondary metabolites can be used for quality control. This study aims to determine the effect of differences in growing locations on levels and secondary metabolites in the Tuban, Bojonegoro, and Ngawi regions. The method used is Thin Layer Chromatography (TLC) followed by the Orange 3.32 Principal Component Analysis (PCA) chemometric method to obtain the grouping and concentration of each sample. The results of the TLC separation obtained 10 sample spots from the Tuban area, 10 sample spots from the Bojonegoro area, and 13 sample spots from the Ngawi area. PCA multivariate analysis explained that the total data variation was 91% (PC 1= 52.5%; PC 2= 38.5%). These results indicate that there are differences in the levels and groups of active compounds with different Area Under Curve (AUC) values for each sample of the earring leaf extract.
Co-Authors Abdul Hakim Abrilia, Putri Riza Akhmad Sabarudin Akyunul Jannah Alfi Istiqomah, Alfi Andiartama, Amanda Milano Anggraini, Maulinda Putri Datin An Nisa Sukmawati Deva Krisna Kadarani Dewi Yuliani Dewi Yuliani, Dewi Ella Wulandari Fadhila, Salma Iza Fatati, Asnal Fiddaroini, Saidun Fiisyatirodiyah, Fiisyatirodiyah Hidayaty, Alfianita Nuril Himmatul Barroroh Ifaridatul Chusna, Ifaridatul Indradmojo, Chrystiaji Laksono, Muhammad Teguh Latifah Latifah M. Rafi Madaniyah, Luailik Moh. Farid Rahman Muhammad Teguh Laksono Muhibbatul Husnah Muti'ah, Ro’ihatul Muti'ah, Roihatul Nasifah, Ilmin Rachmawati Ningsih Rachmawati Ningsih Roihatul Muti'ah Roihatul Mutiah Roihatul Muti’ah Shofiyullah, Muhammad Susi Nurul Khalifah Tri Kustono Adi Yani Triastutik, Yani