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All Journal HAYATI Journal of Biosciences Buletin Peternakan Jurnal Veteriner JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Ilmu Ternak Veteriner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Kedokteran Hewan

herdis herdis

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Author-ID : 280004

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Improvement of frozen semen quality of Garut Sheep through the addition of α-tocopherol into yolk egg-skim milk diluent Herdis Herdis; Kusuma Kusuma; M Surachman; M Riza; I K Sutama; I Inounu; B Purwantara; I Arifiantini
Jurnal Ilmu Ternak dan Veteriner Vol 7, No 1 (2002): MARCH 2002
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The sperm is very fragile to lipid peroxide reaction, that it can easily broken during the process of freezing. To eliminate this consequences an antioxidant agent added into the extender. A research was done to observe the effect of antioxidant agent α-tocoferrol and butylated hydroxytoluene (BHT) presence in the extender on the quality of frozen semen. Once week, semen from six male Garut sheep ages about 2.5 years old was collected using artificial vagina and egg yolk skim-milk diluent used as the extender. The semen were treated in egg yolk skim-milk diluent without antioxidant as control, in egg yolk skim-milk diluent with α- tocoferrol 0,2 g/100 ml diluent and in egg yolk skim-milk diluent with butylated hydroxytoluene 0,2 g/100 ml diluent. The after thawing observation shown that in egg yolk skim-milk diluent with α- tocoferrol had life percentage (75.0 ± 3.5% vs 64.8 ± 7.8%) and membrane intact percentage (65.8 ± 6.8 % vs 55.2 ± 8.3%) significantly higher than control (P<0,05) but insignificantly different from with BHT addition. The presence of α-tocoferrol in the diluent, the motility percentage consideraly higher (P<0.05) than (45.8 ± 3.8%) using BHT addition (40.0 ± 4.5%) but not different from control (41.7 ± 4.1%); while acrosomal intake percentage after α-tocoferrol (54.8% ± 3.3%) expressively higher (p,0.05) than BHT addition (49.7 ± 3.6%) or control (49.8 ± 3.5%). In conclusion the presence of α-tocoferrol in the diluent could improve the quality of Garut sheep frozen semen. Key words: Antioxidant, sperm, Garut sheep

Hubungan antara Konsentrasi Testosteron, Lingkar Skrotum, Libido dan Kuantitas Sperma Pejantan Sapi Pasundan Santoso Santoso; Herdis Herdis; Raden Iis Arifiantini; Asep Gunawan; Cece Sumantri
Jurnal Veteriner Vol 22 No 3 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Sapi pasundan merupakan salah satu sumber daya genetik sapi potong lokal Indonesia yang perlu dikembangkan dan dilestarikan. Penelitian bertujuan untuk menganalisis hubungan konsentrasi testosteron, lingkar skrotum, libido, dan kuantitas sperma pada sapi pejantan pasundan. Sepuluh ekor sapi pasundan berumur 3-6 tahun dengan bobot badan (BB) 380-430 kg digunakan pada penelitian. Konsentrasi hormon testosteron dianalisa dengan metode enzyme-linked immunosorbent assay (ELISA). Libido diamati dari waktu pejantan didekatkan ke kandang atau tempat koleksi semen hingga kontak pertama dengan betina (courtship), waktu pejantan melakukan percumbuan (dating) sampai menaiki betina (mounting), waktu pejantan dating dan pertama kali menaiki betina sampai terjadi ejakulasi. Pengukuran lingkar skrotum menggunakan pita ukur. Semen dikoleksi menggunakan vagina buatan dan dievaluasi karakteristik volume semen dan konsentrasi sperma. Data dianalisa dengan korelasi regresi linear. Rataan konsentrasi testosteron adalah 13,38±0,21 ng/mL. Konsentrasi testosteron (r = -0,661) dan umur (r = 0,681) menunjukkan korelasi negatif dengan waktu courtship (P<0,05), waktu mounting berkorelasi positif dangan waktu ejakulasi (r = 0,756, P <0,05). Korelasi tidak ditemukan antara konsentrasi testosteron dengan umur (r = 0.116), lingkar skrotum (r = 0.328), peningkatan (r = 0.249), ejakulasi (r = -0.179), volume semen (r = 0.243), dan konsentrasi sperma (r = -0.116). Penelitian menyimpulkan konsentrasi testosteron dan umur berkorelasi negatif dengan courtship dan waktu mounting berkorelasi positif dangan waktu ejakulasi.

Pengaruh Penambahan Trehalosa dalam Pengencer Tris terhadap Kualitas Semen Cair Domba Garut (Ovis aries) Herdis Herdis; Maman Surachman; Muhammad Rizal; Arief Boediono; Yulnawati Yulnawati
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 23, No 1 (2006)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2006.23.1.142

This study was aimed to examine the quality of chilled-cement of Garut ram which was diluted with Tris extender and various trehalose concentrations. Cement was collected from three mature rams using an artificial vagina once a week. After initial evaluation, the cement was divided into four parts and diluted with Tris extender only (control), Tris extender + 0.20% (TR0.2), Tris extender + 0.40% (TR0.4), and Tris extender + 0.60% (TR0.6) trehalosa respectively. The extended-cement was stored at 5oC. Parameters of chilled-cement quality examined were percentage of sperm motility, live sperm, and intact plasma membrane (IPM), which were evaluated every day for four days. The results showed that addition of trehalose did not significantly improved the percentage of sperm motility, live sperm, and IPM (P>0.05) of Garut ram sperm stored in refrigerator at 5oC for 4 days. In conclusion, the addition of trehalose does not enhance the quality of chilled-cement of Garut ram.

EPIDIDYMAL SPERMATOZOA QUALITY OF ETAWA CROSSBREED GOAT IN TRIS EXTENDER SUPPLEMENTED WITH VARIOUS LACTOSE CONCENTRATIONS Riyadhi, Muhammad; Setiawan, Agus; Herdis, Herdis; Rizal, Muhammad
Jurnal Kedokteran Hewan Vol 11, No 1 (2017): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i1.5136

This research was conducted to investigate the effect of various concentrations of lactose supplementation in Tris extender for maintaining the quality of Etawa crossbreed goat epididymal spermatozoa stored at 3-5 C. Semen in the control group was diluted with a tris extender containing 20% egg yolk without lactose. Semen in the test groups was diluted with a tris extender containing 20% egg yolk and added with 0.3% (0.3 g per 100 mL extender) and 0.6% lactose for group TL1 and TL2, respectively. Parameters evaluated of the fresh epididymal spermatozoa were motility, concentration, percentage of live, and abnormality of spermatozoa, while for diluted-spermatozoa were motility and percentage of live spermatozoa. Spermatozoa observation was conducted until it reaches 40% motility. The results showed that the mean percentage of motility, live sperm, concentration, and abnormality of epididymal spermatozoa were 70%; 81%; 3,220x106 cells/mL; and 4.30%, respectively in all group. After dilution, the percentage of motility and live spermatozoa were also 70% and 81.001.58%, respectively in all groups. The decreasing of spermatozoa motility was observed on day 4 of storage, in which percentage of spermatozoa motility in control group (40.000.00%) was significantly lower (P0.05) than those in TL1 (44.002.24%) and TL2(45.000.00%) groups. Percentage of live spermatozoa in control (63.202.68%) was not significantly different (P0.05) than TL1(65.401.95%) and TL2 (65.601.95%). In conclusion, the supplementation of lactose into Tris extender could maintain the epididymal spermatozoa of Etawa crossbreed for 3 days of storage at 3-5 C.

PENAMBAHAN BEBERAPA JENIS GULA DAPAT MENINGKATKAN KUALITAS SPERMATOZOA BEKU ASAL EPIDIDIMIS TERNAK DOMBA (Addition of Various Sugars in Improving Quality of Frozen Thawed Epididymal Spermatozoa of Ram) Herdis, Herdis; Darmawan, I Wayan Angga; Rizal, Muhammad
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5139

ABSTRACT The purpose of this research was to examine the effect of various sugars addition in Tris extender on quality of frozen thawed epididymal spermatozoa of ram. Collected spermatozoa was divided into five tubes and centrifuged at 3,000 rpm for 30 minutes. Supernatants were removed and pellets were diluted with Tris extender (control), Tris extender + 0.4% dextrose (dextrose), Tris extender + 0.4% maltose (maltose), Tris extender + 0.4% lactose (lactose), and Tris extender + 0.4% sucrose (sucrose), respectively. There were no significantly difference among treatment on mean percentages of motile (MS), live (LS) and intact plasma membrane (IPM) after equilibration. Mean percentages of post-thawed MS, LS, and IPM for dextrose (43.0, 53.8, and 53.4%), maltose (44.0, 54.8, and 52.8%), lactose (44.0, 52.8, and 52.8%), and sucrose (44.0, 55.0, and 53.4%) were significantly (P0.05) higher than control (37.0, 44.0, and 40.8%). In conclusion, addition of 0.4% dextrose or 0.4% maltose or 0.4% lactose or 0.4% sucrose in Tris extender increased quality of frozen thawed cauda epididymal spermatozoa of ram.

Various Saccharides Addition of Extender Ram Efficiency to Improve Cryopreservation in Semen Etawah Crossbreed Bucks with Seminal Plasma Replacement nurlatifah, aeni; Sitaresmi, Pradita Iustitia; Hafid, Anita; Mahari, Desiana Ade; Lupitasari, Florentina Bety Indah; Anwar, Rahma Isartina; Santoso, Santoso; Rizal, Muhammad; Herdis, Herdis
Buletin Peternakan Vol 48, No 2 (2024): BULETIN PETERNAKAN VOL. 48 (2) MAY 2024
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v48i2.89390

Phospholipase in buck seminal plasma will trigger a coagulation incident resulting in the demise of the spermatozoa during liquid semen processing using milk or egg yolk substrates diluent. A plasma replacement process is performed to avoid this possibility. The cryopreservation process leads to sperm cell damage due to the freezing process, therefore cryoprotectant agents such as saccharides are required as protective agents. This study aimed to investigate the effect of various types of saccharides on the quality of frozen semen of etawah crossbreed (PE) bucks during cryopreservation and thawing with plasma replacement using Priangan ram semen plasma. Semen was collected using an artificial vagina once a week. Fresh PE bucks semen centrifuged at 3,000 RPM for 30 min. The supernatant (seminal plasma) was removed and replaced in equal volume with Priangan ram seminal plasma. Semen was divided into four treatments: goat semen with sheep semen plasma in Tris diluent (control); control with 0.6% dextrose added (monosaccharides); control with 0.6% sucrose added (disaccharides); control with 0.6% raffinose added (trisaccharides). Semen quality including percentages of motile spermatozoa, live spermatozoa, and intact plasma membrane (IPM) were evaluated after diluted, equilibrated, and thawing, respectively. Results of this study showed that viability and IPM of monosaccharides, disaccharides, and trisaccharides were significantly (P<0.05) higher than control (54.40 and 51.40; 55.00 and 53.60; 55.60 and 52.20 vs 48.40 and 52.20, respectively). The motility of disaccharides was significantly (P<0.05) higher compared to control and other diluent (47.00 vs 41.00, respectively). In conclusion, different types of saccharides were effective in maintaining the quality of etawah crossbreed buck frozen semen. The addition of disaccharides seems more effective compared to monosaccharides and trisaccharides in tris extender on the quality of etawah crossbreed buck frozen semen.

Co-Authors A Boediono Agus Setiawan Anak Agung Gede Sugianthara Anwar, Rahma Isartina Arief Boediono AS Aku, AS B Purwantara B Purwantara Cece Sumantri D, i wayan Angga Darmawan, I Wayan Angga Hafid, Anita I Arifiantini I Inounu I K Sutama I Supriatna Ida Kusuma Kusuma Kusuma Lupitasari, Florentina Bety Indah M Riza M Rizal M Surachman Mahari, Desiana Ade Maman Surachman MR Toelihere, MR Muhammad Riyadhi MUHAMMAD RIZAL N Sandiah, N nurlatifah, aeni PD Sadsoeitoeboen, PD R Amin, R R. Iis Arifiantini RTS Adikara SANTOSO SANTOSO Santoso Santoso Sitaresmi, Pradita Iustitia Siti Zubaidah YULNAWATI YULNAWATI

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