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All Journal HAYATI Journal of Biosciences Buletin Peternakan Jurnal Veteriner JURNAL SAINS DAN TEKNOLOGI INDONESIA Jurnal Ilmu Ternak Veteriner ZOO INDONESIA Animal Production : Indonesian Journal of Animal Production Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Kedokteran Hewan
herdis herdis
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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Journal : Animal Production : Indonesian Journal of Animal Production

 1 
The Effect of Phyto-Lecithin on Preservation and Cryopreservation of Semen: A Review Aku, AS; Sandiah, N; Sadsoeitoeboen, PD; Amin, R; Herdis, Herdis
ANIMAL PRODUCTION Vol 9, No 1 (2007): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

Artificial insemination represents one of technologies in livestock reproduction that can be applied to cattle, sheep, goats and other livestock. Application of livestock reproduction technology includes artificial insemination to increase reproductive efficiency. Semen processing is one critical phase in an artificial insemination program. The use of animal origin ingredient for semen extenders, such as egg yolk and milk, presents a risk of microbial contamination, which lead to the search for alternatives. To increase standard of quality, researchers exploits phyto-lesitin for semen extender and the results showed no significant differences in motility, viability, and acrosomal status of spermatozoa with phyto-lesitin extender when compared to tris-egg yolk-containing extenders. (Animal Production 9(1): 49-52 (2007) Key Words : Phyto-Lechitin, preservation, cryopreservation, semen
Optimalization of Equilibration and Thawing Methode on Freezing Process of Garut Ram Semen (Ovis aries) Herdis, Herdis; Toelihere, MR; Supriatna, I; Purwantara, B; Adikara, RTS
ANIMAL PRODUCTION Vol 7, No 2 (2005): May
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Generally, the problem of semen freezing process  is  cold shock effect and intracelullar change due to water release which is  related to  ice crystal formation. One factor to solve the problem is finding out optimal equilibration time and thawing method so there is only a little  damage spermatozoa during freezing process.  The research has been done  to explore  the  influence of  equilibration time and thawing methode  on  frozen semen quality of garut rams. The results shown that the mean of percentages of progressive motile sperm, percentages of viable sperm, percentages of plasma membrane and acrosomal intact on four hours equilibration (52.50% ;  62.33%; 57.17% and 56.42%)  were not significantly different  (P>0.05) from five hours equilibration (52.27%;  63.67%;  56.92% and 57.58 %)  and six hours equilibration (54.17%; 61.00%; 59.42% and 58.58%) respectively.  The percentages of progressive motile sperm, percentages of viable sperm,   percentages of plasma membrane and  acrosomal intact on the thawing method  on the temperature of 370C  for 30 seconds (53.33% ;  62.39%; 57.94% dan 58.61) were not significantly different  (P>0.05) from the thawing methode  on the temperature of 250C for 45 seconds (52.22% ; 62.28%; 57.72% dan 56.44) respectively.  The conclusion shown that  the treatment of four hours equilibration, five hours equilibration,   six hours equilibration and also  the treatment of thawing method  on the temperature of 370C   for 30 seconds and the thawing methode  on the temperature of 250C   for 45 seconds do not have effect on garut ram freezing semen quality. (Animal Production 7(2): 74-80 (2005) Key  Words : Equilibration, Thawing, Semen, Garut ram
Viabilility of Rams Epididymal Sperm after Preservation in Low Temperature (5oc) Rizal, M; Herdis, Herdis; Boediono, A
ANIMAL PRODUCTION Vol 6, No 1 (2004): January
Publisher : Universitas Jenderal Soedirman, Faculty of Animal Science, Purwokerto-Indonesia

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Abstract

The purpose of this research was to evaluate the viability of ram epididymal sperm collected from fresh caudal epididymis (H-0) or after storage in low temperature (5oC, in refrigerator) for one (H-1), two (H-2), and three (H-3) days.  Collected sperm were diluted in modified Tris extender and they were preserved in refrigerator up to four days.  The viability of diluted sperm was evaluated daily base on motility and sperm live.  Results indicated that mean sperm concentration after sperm diluted with 0.05 ml Tris extender of caudal epididymis was 2745 million/ml. Sperm motility and percentage of live for H-0 (71.25% and 82.83%) and H-1 (70.00% and 79.17%) were significantly higher (P<0.05) than H-2 (61.25% and 69.83%) and H-3 (51.67% and 66.17%).  Percentages of sperm motility and live of diluted sperm and preserved in refrigerator for H-0 were significantly higher (P<0.05) than H-1, H-2, and H-3.  These results showed that epididymal sperm collected from caudal epididymis up to three days of preservation (without further storage of the diluted sperm) could be used for artificial insemination or in vitro fertilization programs.  Diluted sperm of H-0 and H-1 could be preserved in refrigerator for two days and H-2 for one day. (Animal Production 6(1): 30-36 (2004) Key Words: Epididymal Sperm, Viability, Rams
Co-Authors A Boediono Agus Setiawan Anak Agung Gede Sugianthara Anwar, Rahma Isartina Arief Boediono AS Aku, AS B Purwantara B Purwantara Cece Sumantri D, i wayan Angga Darmawan, I Wayan Angga Hafid, Anita I Arifiantini I Inounu I K Sutama I Supriatna Ida Kusuma Kusuma Kusuma Lupitasari, Florentina Bety Indah M Riza M Rizal M Surachman Mahari, Desiana Ade Maman Surachman MR Toelihere, MR Muhammad Riyadhi MUHAMMAD RIZAL N Sandiah, N nurlatifah, aeni PD Sadsoeitoeboen, PD R Amin, R R. Iis Arifiantini RTS Adikara SANTOSO SANTOSO Santoso Santoso Sitaresmi, Pradita Iustitia Siti Zubaidah YULNAWATI YULNAWATI