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All Journal MANAJEMEN HUTAN TROPIKA Journal of Tropical Forest Management Jurnal Ilmu Pertanian Indonesia Jurnal Fitopatologi Indonesia Jurnal Silvikultur Tropika (JST) Jurnal Ilmu dan Teknologi Hasil Hutan TELKOMNIKA (Telecommunication Computing Electronics and Control) Jurnal Penelitian Hutan Tanaman Jurnal Pengkajian dan Pengembangan Teknologi Pertanian Jurnal Penelitian Tanaman Industri Jurnal Sain Peternakan Indonesia Journal of Degraded and Mining Lands Management Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) agriTECH JURNAL KESEHATAN LINGKUNGAN (Journal of Environmental Health) Menara Perkebunan Jurnal Ilmu dan Teknologi Kayu Tropis PERENNIAL Jurnal Penelitian Tanaman Industri (Littri) Bulletin of Computer Science Research CELEBES Agricultural Jurnal Pengabdian Masyarakat Bangsa Journal of People, Forest And Environment
Elis Nina Herliyana
Departemen Silvikultur, Institut Pertanian Bogor, Jl. Lingkar Akademik Kampus IPB Darmaga, Bogor 16680
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Energy Engineering Environmental Science Health Professions Industrial & Manufacturing Engineering Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Veterinary

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Journal : Menara Perkebunan

 1 
Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD) Hayati MINARSIH; Dyah LINGGA NP; TW DARMONO DARMONO; Elis Nina HERLIYANA
E-Journal Menara Perkebunan Vol 79, No 1: Juni 2011
Publisher : INDONESIAN RESEARCH INSTITUTE FOR BIOTECHNOLOGY AND BIOINDUSTRY

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.206 KB) | DOI: 10.22302/iribb.jur.mp.v79i1.72

Abstract

AbstractInformation on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).
Analisis keragaman genetik Ganoderma spp. yang berasosiasi dengan tanaman kakao dan tanaman pelindungnya menggunakan Random Amplified Polymorphic DNA (RAPD) Genetic diversity analysis of Ganoderma spp. associated with cocoa and its shade trees using Random Amplified Polymorphic DNA (RAPD) Hayati MINARSIH; Dyah LINGGA NP; TW DARMONO DARMONO; Elis Nina HERLIYANA
Menara Perkebunan Vol. 79 No. 1: 79 (1), 2011
Publisher : INDONESIAN OIL PALM RESEARCH INSTITUTE

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iribb.jur.mp.v79i1.72

Abstract

AbstractInformation on genetic diversity of Ganoderma spp.causing root rot disease in crops is important to developa proper strategy for the control of Ganoderma disease. Theobjectives of this research were to study the genetic diversityof Ganoderma spp. associated with cacao and its shade trees(Albazia faltacaria, Swietenia mahogani, Adenatheramicrosperma and Leucaena leucocephala) by randomamplified polymorphic DNA (RAPD) analysis. Fourty fivesamples of Ganoderma spp. were used in this research. Theresults showed that DNA amplification using 10 arbitraryoligonucleotide primers produced 220 DNA fragmentsshowing polymorphisms. The cluster analysis showed that 45number of Ganoderma samples had a high variability with thecoefficient value ranged from 0.71 to 0.91. Further analysisusing Winboot software showed that three groups ofGanoderma spp. had a high degree of confidence (>50 %),which were Ganoderma samples from sengon (Paraserianthessp.) of Tasikmalaya, sengon (Paraserianthes sp.) ofPalembang, and mahogany of Jember; whereas the othergroups of samples had a low degree of confidence (<50%).AbstrakInformasi tentang keragaman genetik Ganoderma spp.sebagai penyebab penyakit busuk akar pada tanamanperkebunan sangat diperlukan untuk menerapkan strategiyang tepat dalam upaya perlindungan tanaman perkebunan.Penelitian ini bertujuan untuk mengetahui keragaman genetikGanoderma spp. yang berasosiasi dengan tanaman kakao dantanaman pelindungnya (sengon, mahoni, saga dan lamtoro)dari berbagai wilayah di Indonesia menggunakan penandamolekuler random amplified polymorphic DNA (RAPD).Sebanyak 45 sampel Ganoderma spp digunakan dalampenelitian ini. Amplifikasi DNA dengan 10 primer terpilihmenghasilkan 220 fragmen DNA yang menunjukkan adanyapolimorfisme. Hasil analisis menunjukkan adanya keragamanyang cukup tinggi di antara sampel Ganoderma spp. daripohon inang dan wilayah yang berbeda, dengan nilaikoefisien 0,71-0,91. Berdasarkan analisis bootstrapdiketahui bahwa tiga kelompok sampel Ganoderma spp.memiliki tingkat kepercayaan yang tinggi (>50 %) yaitukelompok Ganoderma spp. yang berasosiasi dengan pohonsengon asal Tasikmalaya, sengon Palembang, dan mahoniJember; sedangkan pengelompokan lainnya menunjukkmenunjukkan tingkat kepercayaan yang rendah (<50 %).
Co-Authors Abdul Muhyi ABDUL MUNIF ABDUL MUNIF Abdul Munif Abdurachman Syafiih Abu Bakar, Palah Achmad Achmad . Achmad . Achmad . Achmad ; Achmad Achmad ACHMAD ACHMAD Achmad Lisdar Adisti Permatasari Putri Hartoyo Agus Setiawan Ai Rosah Aisah Akhir, Jumadil ANANG PRANOTO HIDAYAT Ananta Kusuma Amanda Andi Sukendro Andrea Ajeng Eirenne Kristianti Anggi Febry Setyanie Putri Daulay Anisa Tri Harjanti Ardiansyah Putra Ariana Ariana Arief B. Witarto Arief Noor Rachmadiyanto Arif Ravi Wibowo Arzyana Sunkar Asmoro, Prayogo Probo Azahra, Kharisma Aulia Benyamin Dendang Daniswati, Nisa Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Darmono Taniwiryono Deasy Putri Permatasari Dewi Sukma Dodi Nandika Dwierra Evvyernie Dyah LINGGA NP Erianto Indra Putra Eti Artiningsih Octaviani Fatin Hanifah Felliks Tampinongkol, Felliks Fitri Kurniawati Fuzy Yustika Manik Gustan Pari Hanifah Nuryani Lioe Hayati Minarsih Hayati Minarsih Hidayati, Syafitri Hidayatullah, Deden Hutagalung, Labana I. Sudirman Iga Dwi Syahrani Illa Anggraeni Irdika Mansur Irfan Jelata, Tedi Irfan Kemal Putra Irma Badarina Iskandar Z Siregar Isroi Isroi Ivan Permana Putra Kulsum, Fifit Kultsum, Fifit Kunio Tsunoda Laila Fithri Maryam Libranika Dwi Miswati Liza Sakbani Lufthi Rusniarsyah, Lufthi Lul Godi, Rizal Lutfi Hanafi Melly Br Bangun Mira Febrianti Muhammad Alam Firmansyah Nabawiah, Safira Nifa Hanifa Noor Rachmadiyanto, Arief Nurulhaq, Muhammad Iqbal OSICA ASNO FERINA YURTI Ratna Jamilah Reny Meisetyani Reza Pamunca, Airres Rezeka Amalia Rizki Nugraheni Amaliasuci Rossy Nurhasanah Safira Nabawiah Santiyo Wibowo Sarah Diana Yulianti Shodiq Syifaudin, Ikhwan Silviana Arsyad Siti Badriyah Rushayati Soetrisno Hadi Sri Listiyowati Sri Wahyuni, Devi Sri Wilarso Budi Surono Suryo Wiyono Tiara Antika Tjahja Muhandri Toto Toharmat TW DARMONO DARMONO Wartaka Wartaka ; Wasrin Syafii Yurico Bakhri Yusuf Sudo Hadi