Article Per Year (5 Year)
p-Index From 2021 - 2026
1.503
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner EKSAKTA: Journal of Sciences and Data Analysis Jurnal Veteriner Indonesian Journal of Biotechnology JURNAL KAJIAN VETERINER The International Journal of Tropical Veterinary and Biomedical Research
Heru Susetya
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Public Health Veterinary

Published : 23 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search
Journal : Jurnal Veteriner

 1 
Tapak Perlekatan Reseptor Virus Flu Burung yang Diisolasi dari Berbagai Unggas Sejak tahun 2003 sampai 2008 (RECEPTOR BINDING SITE OF AVIAN INFLUENZA VIRUS H5N1 ISOLATED FROM VARIOUS POULTRIES SINCE 2003 TO 2008) Michael Haryadi Wibowo; Charles Rangga Tabbu; Widya Asmara; Heru Susetya
Jurnal Veteriner Vol 13 No 2 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.498 KB)

Abstract

Avian Influenza (AI) is an infectious disease in poultry, caused by type A of avian influenza virus(AIV), in the family of Orthomyxoviridae. Almost all birds’ species are sensitive to the AI. Beside theability to infect various species of poultry. AIV type A has a wide range of host including all bird species,mammals, dan human. Today some scientists reported that the cases of AI in mammals, including humansare increasing. This condition suggests that the AI virus circulated in the field may have some mutationsin the amino acid determinants responsible receptor binding site (RBS). A research was therefore designedto investigate the molecular level of HA gen fragment responsible for receptor binding site of AIV isolatedfrom various poultry since 2003 to 2008. Molecular characterization was based on the amplification ofreceptor binding site of HA gene by reverse transcriptase polymerase chain reaction (RT-PCR). All RTPCRof HA gene positive products were sequenced to determine the nucleotide composition at the targetedfragment. Sequences yielded were analyzed by program Mega 4.0 versions, including multiple alignment,deductive amino acid prediction, and establishment of phylogenetic tree. The results show that all AIVisolates could be determined of some conserved amino acids residues responsible for RBS which indicatethe binding preference of avian like receptor, sialic acid ? 2, 3 galactose except isolate A/Layer/Jabar/MHW-RBS-02/2008 which could be found a deletion of amino acid at position of 129 dan mutation of 151isoleucine into threonine. Phylogenetic study showed that clustering of AIV did not base on species of birdor geographic origin of AI viruses which were studied.
Diagnosis and Molecular Marker Analysis of Bali’s Rabies Virus Isolates (DIAGNOSIS DAN ANALISIS PENANDA MOLEKULER VIRUS RABIES ISOLAT BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; I Gusti Ngurah Kade Mahardika; Helen Scott-Orr
Jurnal Veteriner Vol 15 No 3 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (352.604 KB)

Abstract

The direct fluorescent antibody test (dFAT) was recommended by both World Health Organization(WHO) and Office International des Epizooties (OIE) as a standard diagnostic technique for rabies. Sincethe outbreak of rabies in Bali, it was ascertain the importance to develop a reverse transcriptase-polymerasechain reaction (RT-PCR) technique with specific primers as an alternative diagnostic method. The aim ofthis study was to develop a RT-PCR technique for rabies diagnosis in animals and find out the molecularmarker of Bali’s rabies virus (BRV) isolates based on the sequence of nucleoprotein (N) gene. Brainsamples were obtained during 2009 from 14 suspected rabid dogs and one cattle, where rabies viruseswere isolated. The dFAT was used to detect the presence of rabies viral antigen. Ribonucleic acid (RNA) ofrabies viruses was extracted with TRIzol reagent. Fragment of N gene was amplified using one-step RTPCRmethod with specifically-designed primer pairs and sequenced using ABI automatic sequencer. Multiplealignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA 4.0program. This study found that twelve out of fifteen animal brain samples confirmed as rabies by dFAT.Similarly, a single band of 1215 bp PCR product for rabies virus was also detected in twelve out of twelve(100%) dFAT rabies positive samples. It is therefore evident that alternative diagnostic of rabies inanimals can be established using RT-PCR technique. The results showed that the RT-PCR has a very highagreement with dFAT. Polymorphic sites of N gene of twelve BRV isolates were identified at the position186, 501, 801, 840, 1068 and 1153. Bali’s rabies virus isolates have conserved amino acid (isoleucine)alterations at position 308 (open reading frame). Isoleucine distinguished between all Bali’s isolates andthe all of isolates from other area of Indonesia and other part of the world. This finding significantlydifferent as compared to other rabies virus isolates from other part of Indonesia or the world documentedon the GenBank. Accordingly it is proposed that it can be used as molecular marker and believed to be thefirst study of molecular marker of rabies virus in Indonesia.
Faktor-Faktor Risiko Rabies pada Anjing di Bali (RISK FACTORS ANALYSIS FOR RABIES INDOGS IN BALI) I Nyoman Dibia; Bambang Sumiarto; Heru Susetya; Anak Agung Gde Putra; Helen Scott-Orr
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.428 KB)

Abstract

The efforts to eradicate rabies in Bali have been done for more than three years. However, therabiescases is still spreading. Thus, rabies virus continues to infect humans. A case-control study wasconducted to identify the risk factors associated with rabid dog in Bali. Cases were defined as dogsconfirmed having rabies by direct fluorescent antibody test (dFAT). Determination of sample amount ineach district was taken proportionally and samples were taken by using simple random sampling. A totalof 51 rabid dog cases between 2010 and 2011 and 102 uninfected rabies dogs as control were used in thisstudy. Possible associated factors were obtained by doing questionnaire. The data were subsequentlyanalyzed using chi-square (X2) and odds-ratio (OR) for possible association, which were ultimately analyzedby means of logistic regression to build up of model. This study revealed that factors associated with rabiddog were the status of rabies vaccination (X2= 55.538; P= 0.000; OR= 19.133; 95% CI= 8.015<OR<45.678),contact with other dog (X2= 43.659; P= 0.000; OR= 12.551; 95% CI= 5.541<OR<28.430),condition of dog(X2= 9.994; P= 0.002; OR= 3.019; 95% CI= 1.504<OR<6.058),number of raised dog (X2= 9.284; P= 0.002;OR= 2.962; 95% CI= 1.455<OR<6.027), and veterinary care (X2= 5.258; P= 0.022; OR= 2.444; 95% CI=1.125<OR<5.310). It was found an appropriate logit model to estimate probability of rabid dog events inBali province as follows : Logit Pr (rabies=1| x) = - 4.413 + 3.919 (status of rabies vaccination) + 3.457(contact with other dog). This study is expected to be used as a reference in order to improve rabies controleffectiveness in Bali.
Co-Authors Anak Agung Gde Putra Arselia Kartika Sari Astri Dwyanti Tagueha Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bawono, Gigih Charles Rangga Tabbu Charles Rangga Tabbu Daniel Happy Putra Dewi Anggreini Doddi Yudhabuntara Doddy Yudhabuntara Dyah Ayu Widiasih Dyah Ayu Widiasih F. Lintang R. Daru Maya Gallantiswara, Putu Cri Devischa Hani Muhamad Mardani Haryadi M. Wibowo Haryadi M. Wibowo, Haryadi M. Helen Scott-Orr I Gusti Ngurah Kade Mahardika I Nyoman Dibia Indri Permatasari Ito Naoto Ito Naoto, Ito Khrisdiana Putri Khrisdiana Putri Khrisdiana Putri, Khrisdiana M.Th. Khrisdiana Putri Makoto Sugiyama Makoto Sugiyama, Makoto Michael Haryadi Wibowo Muchammad Taufik Kurniawan Niken Respati Maharani Nobuyuki Minamoto Nobuyuki Minamoto, Nobuyuki Prisha Lini Sivalingham Rahmi, Siwi Meylina Roza Azizah Primatika Roza Azizah Primatika Roza Azizah Primatika, Roza Azizah Salim, Devi Andreani Setyawan Budiharta Silalahi, Gustaf Eifel Toha, Larry Richard Wellem Tri Untari Tri Untari Widagdo S. Nugroho Widagdo Sri Nugroho Widagdo Sri Nugroho Widya Asmara Yatri Drastini Yudhanto, Setyo Yuli Miswati