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All Journal Jurnal Tumbuhan Obat Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) e-Journal Pustaka Kesehatan Pharmaciana: Jurnal Kefarmasian Jurnal Pengembangan Pendidikan Jurnal Riset Kimia UNEJ e-Proceeding ALCHEMY Jurnal Penelitian Kimia JSFK (Jurnal Sains Farmasi & Klinis) JPSCR : Journal of Pharmaceutical Science and Clinical Research Jurdimas (Jurnal Pengabdian Kepada Masyarakat) Royal Jurnal Ilmu Kefarmasian Indonesia Journal of Pharmaceutical Care Anwar Medika Berkala Penelitian Hayati Journal of Tropical Pharmacy and Chemistry
Mochammad Amrun Hidayat
Fakultas Farmasi Universitas Jember
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Energy Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences

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Journal : e-Journal Pustaka Kesehatan

 1 
Uji Aktivitas Antibakteri Ekstrak Etanol Daun Waru Gunung (Hibiscus macrophyllus) dan Fraksinya terhadap Staphylococcus aureus Afif Rifqie Maulana; Bawon Triatmoko; Mochammad Amrun Hidayat
Pustaka Kesehatan Vol 9 No 1 (2021): Volume 9 No.1, 2021
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v9i1.16432

Abstract

Infection is one of the main causes of world health problems, especially in developing countries like Indonesia, one of which is an acute respiratory infection caused by bacteria such as Staphylococcus aureus. Therapeutics are used for the treatment of infections today with antibiotics. However, there are many cases of bacterial resistance to antibiotics that need to develop alternatives derived from plants. One of the plants that have antibacterial activity is the leaves of Waru Gunung (Hibiscus macrophyllus). The purpose of this study was to determine whether the ethanolic extract of H. macrophyllus leaves and their fractions have antibacterial activity against S. aureus. The method used in this antibacterial activity test is the disk diffusion method with a test concentration of 5%, 10%, 15%, 20%, and 25%. The results of this study indicate that all test samples have antibacterial activity except in the water fraction. The highest antibacterial activity was obtained in the ethyl acetate fraction which attracted semipolar compounds in the ethanolic extract of H. macrophyllus leaves. The antibacterial activity of the test samples in sequence from high to low includes ethyl acetate fraction, ethanolic extract, n-hexane fraction, and water fraction.
Formulasi dan Penentuan Stress Testing Sediaan Krim M/A dan A/M Ekstrak Etanol Edamame (Glycine max) (Formulation and Stress Testing of Ethanol Extract Edamame (Glycine max) O/W and W/O Cream) Oktavia Catur Xenograf; Budipratiwi Wisudyaningsih; Siti Muslichah; Moch. Amrun Hidayat
Pustaka Kesehatan Vol 3 No 3 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Edamame is a species of soybean (Glycine max) that contains isoflavone which has tyrosinase inhibitory activity. The aims of this study were to determine the effect of formulation on genistein content and the effect of storage temperature (25 °C and 50 °C) on oil in water (o/w) and water in oil (w/o) cream physicochemical characteristics (viscosity, spreadability, and pH). Genistein level was determined using thin layer chromatography (TLC) densitometry method and stability test was determined using stress testing method. The result showed that formulation gave no effect on genistein content in o/w and w/o cream with recovery values of 95.4178 ± 2.2815 % and 101.8276 ± 5.0063 %, respectively. The storage at 25 °C gave no effect on physicochemical characteristic, while the storage at 50 °C decreased viscosity value and increased spreadability value of cream.   Keywords: edamame, isoflavone, cream, stress testing
Uji Aktivitas Protein Larut Air Umbi Porang (Amorphophallus muelleri Blume) terhadap Escherichia coli dan Staphylococcus aureus Putu Gita Maya Widyaswari Mahayasih; Tri Handoyo; Mochammad Amrun Hidayat
Pustaka Kesehatan Vol 2 No 2 (2014)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

The objective of this study was to determine antibacterial activity of water soluble protein of Porang (Amorphalus muelleri Blume) tuber. The water soluble protein extract was tested against both Eschericia coli and Staphylococcus aureus using filter paper disk method. The inhibition zone of bacterial growth was calculated for both of bacterial cultures. It was found that water soluble protein exhibited antibacterial activity at linear range 0.3-1.9 μg and 0.1-1.9 μg for E. coli and S. aureus respectively, sugesting that S. aureuswas more susceptible than E. coli to this protein. From the electrophoretic profile using 12.5% sodium dodecyl sulfate (SDS) and comassie blue staining (CBS), two protein bands were observed with molecular weight 17 and 19 kDa respectively. The water soluble protein were further evaluated using periodic acid staining (PAS). Pink band with molecular weight 250 kDa and > 250 kDa was found in PAS profile, sugesting the availability of carbohydrate molecule in water soluble protein extract of porang tubers.
Aktivitas Inhibitor α-Glukosidase Ekstrak Etanol Daun Kenitu (Chrysophyllum cainito L.) (α-Glucosidase Inhibitor Activity of Ethanol Extract Kenitu Leaves (Chrysophyllum cainito L.)) Fatimatuz Zuhro; Endah Puspitasari; Siti Muslichah; Moch. Amrun Hidayat
Pustaka Kesehatan Vol 4 No 1 (2016)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Kenitu (Chrysophyllum cainito L.) grows in Jember region, East Java, with several variants which can be distinguished from the color and shape of the fruit. They are small round (BK), large round (BB), green oval (HL), and red round (MB). Kenitu leaves have been shown to provide antidiabetic activity in vivo, but the molecular mechanism still unknown yet. One of the oral antidiabetic is a class of α-glucosidase inhibitor. This research aimed to test the activity of Saccharomyces cerevisiae α-glucosidase inhibitor of several variants of kenitu leaves in Jember, as well as phytochemical screening. Simplicia powder was extracted using 70% ethanol with ultrasonication method to obtain a thick extract. α-glucosidase inhibitor activity test was carried out on the negative control solution (without extract) and the sample solution (extract) using chromogenic method. Phytochemical screening was done by TLC and tube test. IC50 value of ethanol extract kenitu leaves variant BK, BB, HL, and MB were 5.476 ± 0.039, 4.869 ± 0.018, 9.465 ± 0.012, and 11.836 ± 0.048 µg/ml, respectively. Based on the LSD test, IC50 values between variants of kenitu leaves were significantly different (p < 0,05). Phytochemical screening result showed that ethanol extract kenitu leaves contain saponin, triterpenoids, flavonoids, and phenolic compounds. Those compounds were suspected to contribute on α-glucosidase inhibitor activity, but more study still needed to isolate the active compounds and evaluate each isolate for its α-glucosidase inhibitor activity. Keywords: α-glucosidase inhibitor, kenitu leaves, ethanol extract
Pengaruh Ekstrak Metanol, Fraksi N-Heksana dan Fraksi Metanol Biji Pepaya (Carica papaya L.) terhadap Motilitas Spermatozoa Tikus (Effect of the Methanol Extract, Hexane Fraction and Methanol Fraction of Papaya Seeds (Carica papaya L.) on Rat Spermato Novi Prasetyaningrum; Siti Muslichah; Moch Amrun Hidayat
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Lack of contraceptive choices which meet the requirements is one of the contributing factors to less participation of man in contraceptive use. This research aimed to study the effectiveness of papaya seeds on rat spermatozoa motility. The rats were separated into 4 groups: negative control group (CMC-Na 1%), methanol extract of papaya seeds (100 mg/kg BW) treatment group, n-hexane fraction of papaya seeds (100 mg/kg BW) treatment group, and methanol fraction of papaya seeds (100 mg/kg BW) treatment group. Each treatment group consisted of 5 rats. Each group was administrated orally for 20 days. On 21st day, the rats were sacrified, the spermaatozoa were and were observed on their motility. The data then was anallyzed using Anova followed with LSD. The sperms which moved progressively in negative control, methanol extract, n-hexane fraction and methanol fraction were 67.6%, 29.8%, 38% and 10.6% respectively the methanol fraction of papaya seeds is more potent in reducing sperm motility. Keywords: antifertility, papaya seeds, spermatozoa motility
Pengembangan Sensor Antioksidan berbasis Kertas Zonamikro dengan Imobilisasi DPPH pada Sampel Ekstrak Tanaman Muhammad Fantoni; Indah Yulia Ningsih; Moch. Amrun Hidayat; Bambang Kuswandi
Pustaka Kesehatan Vol 11 No 2 (2023): Volume 11 No.2, 2023
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/pk.v11i2.12958

Abstract

Development of a paper-based antioxidant sensor using DPPH reagent. This sensor is made using a screen-printing method to create a 5 mm diameter detection zone which is immobilized with DPPH reagents. The analysis was carried out in one step by immobilizing antioxidants/samples in the detection zone. After reduction by antioxidants, DPPH radicals become stable DPPH molecules, resulting in a change in color from purple to pale yellow. The purple intensity of DPPH was inversely proportional to the antioxidant activity of the sample and was measured using the help of ImageJ software. The optimal conditions for using DPPH reagents at a concentration of 5 mM and the volume in each detection zone were 3 µL. Characterization of this sensor analysis was carried out on gallic acid with the response time in the 12-minute, linearity with r = 0,9895, detection limit (LOD) value 0,0349 mM GAE, the quantitation limit (LOQ) value was 0,1164 mM GAE, with precision <2% (RSD), and meets the accuracy range of 97-103%. This sensor is then validated against DPPH spectrophotometry UV-Vis by analyzing antioxidant activity from plant extracts. The results showed no significant differences for the gallic acid equivalent for all samples obtained from the two methods at a confidence level of 97-103%, indicating that the method developed could be relied upon to analyze antioxidant activity from real samples. Finally, the paper-based antioxidant sensor is known to be stable for three days when stored in the refrigerator (2- 4 °C), stable for 2 hours at room temperature (25ºC), and makes paper sensors easy to use for end users.
Co-Authors Afif Rifqie Maulana Annisa Ragdha Eka Nuryuanda Antonius Nugraha Widhi Pratama, Antonius Nugraha Widhi Bambang Kuswandi Bambang Kuswandi Bambang Kuswandi Bambang Kuswandi Bawon Triatmoko Dewi Dianasari Diana Holidah Dianasari, Dewi Endah Puspitasari Endah Puspitasari ESTIKA YUNINDARWATI Evi Umayah U Evi Umayah Ulfa Fatimatuz Zuhro Fatimatuzzahra Fatimatuzzahra Husnatul Ayniah Ika Barokah Suryaningsih Ika Puspita Dewi Indah Yulia Ningsih Indah Yulia Ningsih Lisa Kusuma Wardhani Maidy Frista Rosanti Mandasari, Virda Fitra Muhammad Fahmi Naufal Muhammad Fantoni Novi Prasetyaningrum Noviani Tri Wahyuning Nuri Nuri Nuri Nuri Oktavia Catur Xenograf Panida, Rahma Riska Putu Gita Maya Widyaswari Mahayasih Sari, Dewi Diana Seniarta, I Wayan Tanfidz Alishlah TRI HANDOYO Ubaidillah Abdel Barsyaif Ulfa Elfiah Umiyah Umiyah Weka Sidha Bhagawan YUNITA