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All Journal Jurnal Tumbuhan Obat Indonesia Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Prosiding Seminar Nasional Sains Dan Teknologi Fakultas Teknik Indonesian Journal of Cancer Chemoprevention Jurnal Ilmu Farmasi dan Farmasi Klinik (Journal of Pharmaceutical Science and Clinical Pharmacy) Pharmascience Indonesian Journal of Chemical Science Jurnal Profesi Medika: Jurnal Kedokteran dan Kesehatan PHARMACY: Jurnal Farmasi Indonesia (Pharmaceutical Journal of Indonesia) PengabdianMu: Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Ilmiah Cendekia Eksakta Media Farmasi Indonesia Jurnal Farmasi Sains dan Praktis Jurnal Kefarmasian Indonesia
Devi Nisa Hidayati
Universitas Wahid Hasyim
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Economics, Econometrics & Finance Engineering Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Mechanical Engineering Medicine & Pharmacology Public Health Social Sciences Other

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Journal : Pharmascience

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Aktivitas Sitotoksik dan Induksi Apoptosis dari Ekstrak Etanol Kulit Apel Hijau (Pyrus malus L.) terhadap Sel MCF-7 Syahrul Iqbal Setiawan; Erika Indah Safitri; Devi Nisa Hidayati; Laili Nailul Muna
Jurnal Pharmascience Vol 8, No 2 (2021): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v8i2.10729

Abstract

Ekstrak kulit apel hijau (Pyrus malus L.) mengandung senyawa Quercetin yang mampu menurunkan ekspresi Bcl-2. Penelitian ini bertujuan untuk mengetahui aktivitas sitotoksik dan induksi apoptosis ekstrak etanol kulit apel hijau (EEKAH) pada model sel kanker payudara MCF-7. Serbuk kulit apel hijau diekstraksi menggunakan pelarut etanol 70% dengan metode ultrasonik. Uji sitotoksik terhadap sel kanker payudara MCF-7 dengan metode MTT Assay pada konsentrasi EEKAH yaitu 31,25; 62,5; 125; 250; 500 dan 1000 µg/mL. Hasil uji sitotoksik dihitung nilai IC50menggunakan regresi linier. Pengamatan induksi apoptosis dengan konsentrasi ½ IC50(442,5 μg/ml) dan IC50 (885 μg/ml) menggunakan flowcytometry. Analisis data dianalisis menggunakan analisis statistik One Way Anova. Hasil penelitian menunjukan bahwa EEKAH memiliki aktivitas sitotoksik dengan nilai IC50 sebesar 885 µg/ml. Persentase rata-rata fase apoptosis total EEKAH pada konsentrasi ½ IC50sebesar 30,2% (p>0,05) dan IC50 sebesar 47,9% (p<0,05) dibandingkan kontrol sel sebesar 12,7%. EEKAH mampu menginduksi apoptosis terhadap sel MCF-7. Kata kunci: MCF-7, Pyrus malus L., Sitotoksisitas, Apoptosis  Green apple peel extract (Pyrus malus L.) contains Quercetin compound which decrease Bcl-2 expression. The aims of this study was to determined the cytotoxic activity and apoptosis induction of green apple peel ethanol extract (GASEE) on MCF-7 breast cancer cells. The green apple peel powder was extracted using 70% ethanol solvent by ultrasonic method. Cytotoxic test against MCF-7 breast cancer cells using the MTT Assay method at the GASEE concentration, namely 31.25; 62.5; 125; 250; 500 and 1000 µg/mL. The results of the cytotoxic test calculated the IC50 value using linear regression. Determination of apoptosis induction with a concentration of ½ IC50 (442.5 µg/mL) and IC50 (885 µg/mL) using flowcytometry. Data analysis was analyzed using statistical analysis One Way Anova. The results showed that GASEE has cytotoxic activity with an IC50 value of 885 µg/ml. Average percentage of total apoptosis phase are ½ IC50 concentration of 30.2% (p>0.05) and IC50 of 47.9% (p<0.05) than cell control of 12.6%. GASEE can apoptosis induced against MCF-7 cells.
Efek Sitotoksik Ekstrak Etanolik Ocimum basilicum, L. Pada Sel Kanker Payudara Devi Nisa Hidayati; Ibrahim Arifin; Fatimatuz Zahroh; Lina Wahyuni
Jurnal Pharmascience Vol 6, No 2 (2019): Jurnal Pharmascience
Publisher : Program Studi Farmasi FMIPA Universitas Lambung Mangkurat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/jps.v6i2.7353

Abstract

ABSTRAK Pengobatan kanker menggunakan bahan alam terus dikembangkan. Salah satu tanaman yang memiliki efek sitotoksik Ocimum basilicum, L. tujuan penelitian ini adalah mengetahui aktivitas sitotoksik dari ekstrak etanol Ocimum basilicum (EEOB) terhadap sel kanker payudara T47D dan MCF7. Ocimum basilicum diekstraksi menggunakan metode maserasi dengan pelarut etanol 70%. Pengujian aktivitas sitotoksik menggunakan metode MTT assay dengan  seri konsentrasi EEOB 1000; 500; 250; 125; 62.5; 31,25 µg/mL. Hasil uji aktivitas sitotoksik EEOB memperlihatkan nilai IC50 pada sel T47D dan MCF-7 sebesar 399.86 µg/ml dan 387.76 µg/ml. Kata Kunci—Sitotoksik, Ocinum basilicum L., T47D, MCF-7  ABSTRACT             Cancer treatment using natural ingredients continues to be developed. One of the plants that is proven to have cytotoxic activity is basil leaves (Ocimum basilicum, L.). This study aims to determine the cytotoxic activity of ethanol extract of basil leaves (EEBL) on T47D and MCF-7 breast cancer cells. Basil leaves were extracted using maceration  with ethanol 70%. The cytotoxic test was perfomed using MTT assay with various EEBL concentrations: 1000; 500; 250; 125; 62.5; 31,25 µg/mL. The results showed that IC50 of cytotoxic activity in T47D and MCF-7 was 399.86 µg/ml and 387.76 µg/ml respectively. Keywords—Cytotoxic, Ocinum basilicum L., T47D, MCF-7
Co-Authors Alviani, Dwi Lutvi Amalina Firdaus Amaliyah Ayu Nurfitriani Anita Dwi Puspitasari Aqnes Budiarti Aristya Kurniasih Awal Prichatin Kusumadewi Aysah Safitri Cicih Sumiarsih Cicih Sumiarsih Dewi Andini Kunti Mulangsri, Dewi Andini Kunti Dwi Lutvi Alviani Edy Meiyanto Erika Indah Safitri Esa Evinda Fatimatuz Zahroh Fatimatuz Zahroh Fauzizah, Nisa Ferry Sandra Fini Hartanti Fitriasih Fitriasih Fitriasih Fitriasih Gharsina Ghaisani Yumni Hanik Rohmatul Maghfiroh Hartanti, Fini Ibrahim Arifin Ibrahim Arifin Jenie, Riris Istighfari Komariyatun, Siti Laili Nailul Muna Laili Nailul Muna Lalu Ksatria D.S. Lilis Rejeki Lina Wahyuni Lina Wahyuni Lusyana Setiawati Marwan Masita Nur Amalia Putri Mufrod Mufrod Mutia Rahmatun Nenni Pratiwi Nenni Pratiwi Nilam Eka Putri NUR AINI Nur Kussamawari Ardian Nur Rahayu Fitriana Nurul Hidayati Parusiza, Intan Meyta Pia Annisa Pramesti Dewi Rahayu, Putri Ririn Lispita Wulandari Riris Istighfari Jenie risha fillah fithria Rumiyati Rumiyati Salsabiila, Iqna Salsabila, Unik Hanifah Salsabilla Putri Buana Pasha Shafa Fikriyyah Siti Mega Komariah Siti Mega Komariyah Siti Nurikha Siti Wahidatun Nisa Sri Susilowati Sufa , Riisyda Faila Sugiyono - Syahrul Iqbal Setiawan Ulya Felasufah Umroh Mahmudah Umroh Mahmudah Yance Anas Yuni Antika