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All Journal HAYATI Journal of Biosciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Jurnal Hortikultura Jurnal Matematika & Sains AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Acta Pharmaceutica Indonesia BERITA BIOLOGI Jurnal Biodjati Jurnal Mikologi Indonesia Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Makara Journal of Science Archipelago: Jurnal Pengabdian Kepada Masyarakat Iain Ternate Jurnal Kesehatan Bakti Tunas Husada
I Nyoman Pugeg Aryantha
Kelompok Keahlian Mikrobiologi-Genetika-Biologi Molekuler, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Other

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Journal : HAYATI Journal of Biosciences

 1 
Pre-treatment and Suitable Reagent Enabled a Reliable and Consistent for Molecular Detection of Fusarium oxysporum f. sp. cubense Tropical Race 4 (Foc4) Listya Utami Karmawan; Fenny Martha Dwivany; Rizkita Rachmi Esyanti; I Nyoman Pugeg Aryantha
HAYATI Journal of Biosciences Vol. 26 No. 4 (2019): October 2019
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (900.193 KB) | DOI: 10.4308/hjb.26.4.196

Abstract

Fusarium wilt which is caused by the soil-borne fungus, Fusarium oxysporum f.sp. cubense (Foc), is one of important diseases in banana plant. Foc tropical race 4 (Foc4) is the most pathogenic race which could infect various banana cultivars including Cavendish cultivar which was previously considered as resistant cultivar. Molecular detection of Foc using PCR analysis is indispensable to determine the race of Foc. We demonstrate a faster DNA isolation procedure described in previous method by substituting sodium acetate precipitation with ammonium acetate precipitation without affecting the result. Based on our experience, some fungal genomes were troublesome to be amplified. We suggested pre-treatment step prior to amplification procedure by incubating fungal DNA in 65°C for 10 minutes for any samples of fungal genome, including stubborn samples, before mixing into PCR mix reagent. PCR reagents should be tested for stubborn samples since some of the reagents were unable to amplify the desired DNA fragment. Pre-treatment and the choice of robust PCR reagent should be taken into consideration for a reliable and consistent Foc4 molecular detection result.
Endophytic Fungi from Four Indonesian Medicinal Plants and Their Inhibitory Effect on Plant Pathogenic Fusarium oxysporum Nampiah Sukarno; Rohani Cinta Badia Ginting; Utut Widyastuti; Latifah Kosim Darusman; Sihegiko Kanaya; Irmanida Batubara; I Nyoman Pugeg Aryantha; Mashuri Waite
HAYATI Journal of Biosciences Vol. 28 No. 2 (2021): April 2021
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.28.2.152

Abstract

The medicinal plants Centella asiatica, Curcuma xanthorrhiza, Guazuma ulmifolia, and Hydrocotyle verticillata are widely used in Indonesian traditional medicine, but little is known about their associated endophytic fungi. This research aimed to study the diversity of endophytic fungi derived from functional parts of these plants and to evaluate their potential as antifungal agents against the plant pathogenic fungus Fusarium oxysporum. A total of 17 isolates of endophytic fungi were obtained: nine from leaves of G. ulmifolia, three each from leaves of C. asiatica and H. verticillata, and two from rhizomes of C. xanthorrhiza. The genus Colletotrichum was found in all plants studied, but each plant was associated with different species. Colletotrichum aeschynomenes was associated with C. xanthorrhiza, C. siamense was associated with C. asiatica, and C. tropicale was associated with G. ulmifolia and H. verticillata. The species Curvularia affinis, Diaporthe tectonae, Lasiodiplodia mahajangana, Parengyodontium album, Talaromyces trachyspermus, and Speiropsis pedatospora were found only in G. ulmifolia; while Didymella coffeae-arabicae and Muyocopron laterale were found only in H. verticillata. The endophytic fungi showed inhibition activity against F. oxysporum with inhibition values of 6.0-78.9%, T. trachyspermus JBd10 and C. affinis JBd14 gave the highest inhibition activity.
Co-Authors Adhityo Wicaksono Adi Pancoro Adi Pancoro ANDINI WARIH WEDARINGTYAS Andriana Susilowati Ari Widodo Asrul Asrul Darah Ibrahim DEMI TRISTAN DJAJADI Dwiantara, Wahyu Setyaji Enggar Utari Ewi Awaliyati FENNY MARTHA DWIVANY HERI SETIADI Indah Juwita Sari Indria Wahyuni Irmanida Batubara Karlina, Yenni Khusnul, Khusnul KUSUMA, ALI BUDHI Latifah Kosim Darusman LISTYA UTAMI KARMAWAN Mamat Kandar Mashuri Waite Meti Kusmiati Mustafa, Fransisca Astrid Nampiah Sukarno Nuryani Rustaman Panca Jarot Santoso Panca Jarot Santoso Rahmat Ariffudin Reza Raihandhany Rianti Nurpalah Rida Oktorida Khastini Rizkita Rachmi Esyanti Rohani Cinta Badia Ginting Sihegiko Kanaya SONY SUHANDONO Sony Suhandono Sukrasno Sukrasno Sukrasno, Sukrasno Susanti Wulandari Syarifah A. Rashid TATI KRISTIANTI Tutus Gusdinar Kartawinata Utut Widyastuti Wahyu Setyaji Dwiantara WINDI SILVANI JEMSI Yenni Karlina Yuniar Mulyani