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All Journal HAYATI Journal of Biosciences Proceedings of Annual International Conference Syiah Kuala University - Life Sciences & Engineering Chapter Microbiology Indonesia Jurnal Hortikultura Jurnal Matematika & Sains AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Acta Pharmaceutica Indonesia BERITA BIOLOGI Jurnal Biodjati Jurnal Mikologi Indonesia Lumbung: Jurnal Ilmiah Politeknik Pertanian Negeri Payakumbuh Makara Journal of Science Archipelago: Jurnal Pengabdian Kepada Masyarakat Iain Ternate Jurnal Kesehatan Bakti Tunas Husada
I Nyoman Pugeg Aryantha
Kelompok Keahlian Mikrobiologi-Genetika-Biologi Molekuler, Sekolah Ilmu dan Teknologi Hayati, Institut Teknologi Bandung
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Education Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Medicine & Pharmacology Nursing Public Health Other

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Journal : Microbiology Indonesia

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Diversity of Culturable Bacterial in Various Parts of Luwak’s (Paradoxurus hermaprodithus javanica) Gastrointestinal Tract SONY SUHANDONO; HERI SETIADI; TATI KRISTIANTI; ALI BUDHI KUSUMA; ANDINI WARIH WEDARINGTYAS; DEMI TRISTAN DJAJADI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 10 No. 2 (2016): June 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.379 KB) | DOI: 10.5454/mi.10.2.4

Abstract

Luwak coffee is a highly-priced coffee produced exclusively by the palm civet or luwak (Paradoxurus hermaphrodites ssp.). The purpose of this study was to determine the diversity of culturable bacteria in the gastro intestinal tract of luwak. The bacterial isolates were phenotypically characterized by their morphology and molecularly by analysis of their1,500bp 16s rDNA sequence. The results showed that Enterobacter cloacae and Lactobacillus brevis were found all over luwak’s digestive tract. Enterobacter cloacae was the most common species. The most diverse bacterial population was found in small intestine. Seven bacterial generawere successfully identified from the small intestine and colon, compared to only five genera found in the stomach.
Potential MGPB In Optimizing Paddy Straw Mushroom (Volvariella volvacea WW-08) Growth WINDI SILVANI JEMSI; I NYOMAN PUGEG ARYANTHA
Microbiology Indonesia Vol. 11 No. 2 (2017): Juni 2017
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (839.638 KB) | DOI: 10.5454/mi.11.2.2

Abstract

Paddy straw mushroom (Volvariella volvacea) contains high protein content and delicious flavor, makes it highly demand each year. Production of V.volvacea does not merit the requirements due to its limited production. Therefore, approach in increasing production using mushroom growth promoting bacteria (MGPB) are needed. This study aims to obtain MGPB isolate as potential agent to increase V. volvacea strain WW-08 growth. This is experimental research in laboratory that consisted of indigenous bacteria isolation, MGPB screening with dual culture, MGPB inoculum optimization, molecular identification of selected MGPB using 16S rRNA, protein profiling with SDS-PAGE, and fruting body production. Indigenous bacteria obtained from growth medium were 58 isolate, and W34 bacteria at concentration of 106 sel/ml showed most significant result on micellium growth. Sequence of 16S rRNA region showed W34 bacteria is Bacillus cereus. Visualization of SDS-PAGE showed new protein in result of interaction between Bacillus cereus and V. volvacea strain WW-08 with molecule weight of 17kDa. Average of fruting body of V. volvacea strain WW-08 in treatment of B. cereus harvested for 7 days, was 240.19g, whereas without treatment of B. cereus was 82.15g. These findings indicate treatement of B. cereus strain W34 increase V. volvacea WW-08 growth by 300%.
Co-Authors Adhityo Wicaksono Adi Pancoro Adi Pancoro ANDINI WARIH WEDARINGTYAS Andriana Susilowati Ari Widodo Asrul Asrul Darah Ibrahim DEMI TRISTAN DJAJADI Dwiantara, Wahyu Setyaji Enggar Utari Ewi Awaliyati FENNY MARTHA DWIVANY HERI SETIADI Indah Juwita Sari Indria Wahyuni Irmanida Batubara Karlina, Yenni Khusnul, Khusnul KUSUMA, ALI BUDHI Latifah Kosim Darusman LISTYA UTAMI KARMAWAN Mamat Kandar Mashuri Waite Meti Kusmiati Mustafa, Fransisca Astrid Nampiah Sukarno Nuryani Rustaman Panca Jarot Santoso Panca Jarot Santoso Rahmat Ariffudin Reza Raihandhany Rianti Nurpalah Rida Oktorida Khastini Rizkita Rachmi Esyanti Rohani Cinta Badia Ginting Sihegiko Kanaya SONY SUHANDONO Sony Suhandono Sukrasno Sukrasno Sukrasno, Sukrasno Susanti Wulandari Syarifah A. Rashid TATI KRISTIANTI Tutus Gusdinar Kartawinata Utut Widyastuti Wahyu Setyaji Dwiantara WINDI SILVANI JEMSI Yenni Karlina Yuniar Mulyani