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TAMPILAN REPRODUKSI HASIL INSEMINASI BUATAN MENGGUNAKAN SEMEN BEKU HASIL SEXING PADA SAPI PERSILANGAN ONGOLE DI PETERNAKAN RAKYAT Lieyo Wahyudi; Trinil Susilawati; Nurul Isnaini
TERNAK TROPIKA Journal of Tropical Animal Production Vol 15, No 1 (2014): TERNAK TROPIKA
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.415 KB)

Abstract

The purpose of this research was to determine the success of AI using sexed semen results based on NRR, S/C, CR, and rectal palpation were also determine.  The total number of 81 acceptors divided into 3 treatment groups, such as P0 (non- sexed semen) ; P1 (Frozen semen Sexed using White Yolk Sedimentation),  P2 (Frozen semen sexed using Percoll Gradient Centrifugation) . The results showed the NRR percentage of P0, P1 and P2 were between the treatments are P0 of 74.07 ± 12:00%; 65.43 ± 5.66% and 64.19 ±  8:56% respectively. Furthermore the highest of CR was in P0 44%,   P2 25.91% and P1 18.51%. Conclution of this research was the highest percentage of pregnancy was 59.25 %(P0) ,  P1 51.85% and the lowest was in 44.44%. The S/C was in (P0) 2.31 follow by 3.00 in P2 and 3.33 in P1.   Key words : artificial insemination , frozen semen sexed, the percentage of Gestation and feed
PENGARUH PENGHILANGAN RAFINOSA DALAM PENGENCER TRIS AMINOMETHANE KUNING TELUR TERHADAP KUALITAS SEMEN KAMBING BOER SELAMA SIMPAN DINGIN Abdul Rochim; Muhammad Ade Salim; Nurul Isnaini; Trinil Susilawati
TERNAK TROPIKA Journal of Tropical Animal Production Vol 18, No 1 (2017): TERNAK TROPIKA Journal of Tropical Animal Production (JTAPRO)
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (230.049 KB) | DOI: 10.21776/ub.jtapro.2017.018.01.5

Abstract

The objective of this research was to examine the effect of removal raffinose in tris aminomethane egg yolk diluents to semen quality of Boer goat during chilled preservation. This research was carried out at Animal Reproduction Laboratory and Sumber Sekar Laboratory, Animal Husbandry Faculty, Brawijaya University from December 11th 2016 to  January 31th 2017. Semen diluent was divided into two groups, there were P0 (80% Tris Aminomethane + 20% Egg Yolk); and P1 (80% Tris Aminomethane (without raffinose) + 20% Egg Yolk). Parameter of this research was motility percentage, viability percentage, and abnormality percentage. Data of this research was analyzed using paired design t test. The result showed that after five days chilled preservation, was different (P<0,05) on the average motility percentage between P0 (39,50±9,26%) and P1 (43,50±6,26%). There was significantly different (P<0,01) on the average viability percentage between P0 (45,86±15,66%) and P1 (46,38±15,32%). There was significantly different (P<0,01) on the average abnormality percentage between P0 (1,76±0,60%) and P1 (2,31±0,75%). Total motile sperm count after 5 days chilled preservation was different (P<0,05) in P0 treatment and not different (P>0,05) in P1 treatment compared to the expectation value of 40 million sperm/ml. The conclution of this research was the tris aminomethane egg yolk diluent without raffinose could protect of Boer goat semen quality during chilled preservation. Keywords: boer goat semen, tris aminomethane, raffinose, chilled preservation
Hubungan Testosteron dengan Motilitas Spermatozoa Tikus Putih Jantan (Ratus Norwegicus ) Setelah Diberi Senyawa Aktif Daun Beluntas untuk Penyusun Buku Antifertilitas Eko Susetyarini; Aloysius Duran Corebima; Moh Amin; Trinil Susilawati
Belantika Pendidikan Vol 2, No 2 (2019)
Publisher : Kayon Media

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (102.911 KB) | DOI: 10.47213/bp.v2i2.31

Abstract

This research helps study the combination of active combination of beluntas leaf (tannin fraction; combined alkaloid + flavonoid + tannin fraction) with various doses of spermatogenic cell counts, spermatozoa quality, testosterone levels, damage to liver cells and black and white skin. This study is an experimental study, using RAK experimental design with a combination of types of active composition of beluntas leaves Based on the results of the study, it can be concluded that there is a combination of active combinations of beluntas leaves (tannin fraction; combined alkaloid + flavonoid + tannin) fractions with various benefits on the number of spermatogenic cells, individual motility, abnormalities, power live in male white rats; there is no combination for spermatozoa concentration and number of motile spermatozoa, and testosterone levels.Penelitian ini bertujuan untuk mengetahui pengaruh kombinasi pemberian jenis senyawa aktif daun beluntas (fraksi tanin; fraksi gabungan alkaloid+flavonoid+tanin) dengan berbagai dosis terhadap jumlah sel spermatogenik, kualitas spermatozoa, kadar testosteron, kerusakan sel hati dan ginjal tikus putih jantan, Penelitian ini merupakan penelitian eksperimen, menggunakan rancangan percobaan RAK dengan kombinasi perlakuan antara jenis senyawa aktif daun beluntas Berdasarkan hasil penelitian, dapat disimpulkan ada pengaruh kombinasi pemberian senyawa aktif daun beluntas (fraksi tanin; fraksi gabungan alkaloid+ flavonoid+tanin) dengan berbagai dosis terhadap jumlah sel spermatogenik, motilitas individu, abnormalitas, daya hidup pada tikus putih jantan; tidak ada pengaruh kombinasi untuk konsentrasi spermatozoa dan jumlah spermatozoa motil, dan kadar testosterone.
TINGKAT KEBERHASILAN INSEMINASI BUATAN DOUBLE DOSIS PADA SAPI PERSILANGAN ONGOLE DENGAN KUALITAS BERAHI YANG BERBEDA Alifian Ibnu Ansori; Kuswati kuswati; Asri Nurul Huda; Rizki Prafitri; Aulia Puspita Anugra Yekti; Trinil Susilawati
REKASATWA : Jurnal Ilmiah Peternakan Vol 3, No 1 (2021): REKASATWA : Jurnal Ilmiah Peternakan
Publisher : Universitas Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/rekasatwa.v3i1.11820

Abstract

Tujuan dari penelitian ini untuk mengetahui tingkat keberhasilan inseminasi buatan double dosis pada jam ke-8 dan jam ke-16 pada sapi Persilangan Ongole dengan kualitas berahi yang berbeda. Materi dalam penelitian ini menggunakan 25 ekor sapi betina Persilangan Ongole yang dipilih secara purposive. Metode dalam penelitian ini adalah observasi langsung di lapang, dengan menyeleksi sapi betina dengan kriteria BCS >3, umur >1,5 tahun dan telah melahirkan. Penelitian ini menggunakan semen beku pejantan Limousin yang diproduksi oleh Balai Besar Inseminasi Buatan (BBIB) Singosari, Malang. Hasil penelitian menujukkan persentase karakteristik yaitu Non Return Rate (NRR-1, NRR-2) masing-masing 92,31% dan 58,33%, Conception Rate (CR) 38,46% dan 25%, Pregnancy Rate (PR) 53,85% dan 50%. Suhu Vagina 37,0-37,9ºC dan >38,0ºC NRR-1, NRR-2 masing-masing 77,78% dan 57,14%, CR 14,28% dan 33,33%, PR 42,86% dan 55,56%. Lendir servik (ada, basah, sedikit) dan (ada, basah, banyak) NRR-1, NRR-2 masing-masing 89,47% dan 16,67%, CR 31,58% dan 16,67%, PR 47,37% dan 66,67%. Lendir Servik pH 7 dan pH 8 NRR-1, NRR-2 masing-masing 77,78% dan 68,75%, CR 11,11% dan 37,50%, PR 44,44% dan 56,25%. Nilai HD 21-30 dan 31-40 NRR-1, NRR-2 masing-masing 50% dan 82,35%, CR 12,50% dan 35,29%, PR 50% dan 52,94%. Kesimpulan penelitian ini bahwa karakteristik warna vulva merah, suhu vagina >38ºC, karakteristik lendir (ada, basah, banyak), lendir servik pH 8 dan nilai HD 31-40, memberikan persentase kebuntingan yang lebih tinggi.
Effect of Body Condition Score Limousine Crossbred Cow on Cattle Oestrus Response Synchronized using PGF2α Single Dose Muhammad Zainul Hanif; Putri Utami; Aulia Puspita Anugra Yekti; Asri Nurul Huda; Joko Riyanto; Chusnul Hanim; Mashudi Mashudi; Trinil Susilawati
Buletin Peternakan Vol 46, No 2 (2022): BULETIN PETERNAKAN VOL. 46 (2) MAY 2022
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v46i2.72976

Abstract

One  of  the  strategies  to  increase  the  cattle  population  in  Indonesia  is  the  AI (Artificial   Insemination)  method.  The  AI program's  improvement  of   reproductive efficiency can use the oestrus synchronization method. The purpose of this study was to evaluate   the   effect   of   body   weight   and   body   condition   score   on   the   oestrus synchronization  response with  the brand  "lutelyse."  This  research  was  conducted  in  Senggreng  Village,  Sumberpucung  District,  Malang Regency,  This  research  method uses experimental field experiments  with a sampling technique of purposive sampling. This study took a sample of 21, aged 1,5 –5 years, synchronized with PGF2α as much as  5  ml,  which  was  then  carried  out  by  observing  oestrus  behavior  for  24  hours after synchronization.  The  results  showed  that,  the  percentage  of  oestrus in  cows  with body condition  score  (BCS) 4,  which  is  76.92%,  is  lower  than  cows  with  BCS  5,  which  is 100%.  For  the synchronization  distance  with  the  onset  of  oestrus  in  BCS  4  cattle, namely 3802.92 ± 1007.80 (minutes) and BCS 5, namely 4081 ± 884.91 (minutes) with  the results of the conception rate of BCS 4 cattle of 38.46% and BCS 5 of 50%. Based on the results of the study, it was concluded that BCS 5 has a higher success pregnancy rate than BCS 4.
Carcass And Wholesalecut Production Of Brahman Cross (BX) Heifer Kuswati Kuswati; Abdul Muhaimin; Wike Andre Septian; Trinil Susilawati
Jurnal Ilmu dan Teknologi Hasil Ternak (JITEK) Vol. 17 No. 3 (2022)
Publisher : Faculty of Animal Science Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jitek.2022.017.03.7

Abstract

This research aims to determine the quantitative characteristics of Brahman cross heifer cattle's carcass and wholesale cut at different age levels. The number of heifer cattle taken as samples was 104 heifer. The research method used is a case study with the selection of locations by purpose sampling. Direct observation is used to obtain information on age, slaughter weight, carcass weight, wholesale cut weight, and parts thereof (primary, secondary, and manufacturing cut). The data obtained were then analyzed by analysis of variance (ANOVA). If there was a significant difference, it is followed by a further test of the Least Significant Difference (LSD). The results showed that the age levels in slaughter had a significant effect (P<0.05) on the slaughter weight, fresh carcass weight and wilted carcass weight, but had no effect on the percentage of carcass and wholesale cut. The older the age of cattle and the longer the cattle are raised, the higher their body weight will affect the slaughter weight produced, and the higher the slaughter weight, the higher the carcass weight made. Average slaughter weight at different age by Permanent Incisor (PI): PI0 (<18 months), PI2 (18-24 months) and PI4 (24- 36 months). While the average percentage of heifer carcass at different age levels was relatively the same (52,71% : 53,04% : 53.10%). The weight and rate of wholesale cut and its parts are also somewhat the same. The research results show that the slaughter age affects the slaughter weight and carcass weight but not dressing percentage and wholesale cut weight. Brahman cross heifer cattle at PI4 produced optimal carcass weight and wholesale cut.
Study Motility of Ongole Crossbred Semen with Soybean-Based Diluents Stored at Ambient Temperature (24-27 0C) Dian Ratnawati; Gatot Ciptadi; sri rahayu; trinil susilawati
Jurnal Ilmu-Ilmu Peternakan Vol. 33 No. 1 (2023): April 2023
Publisher : Faculty of Animal Science, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jiip.2023.033.01.011

Abstract

Artificial insemination with fresh semen is an alternative that can be done if there is a limitation of liquid nitrogen, bull, and facilities for processing liquid and frozen semen (costly). This study aimed to determine the optimal diluent formula based on CEP-3 (modified/m) and aqueous soybean extract (ASE) to support spermatozoa motility at ambient temperature storage. The research design was completely randomized, with eight treatments and six replications. The diluent formula treatments were as follows: T1 (CEP-3(m)), T2 (CEP-3(m)+10% Egg Yolk/EY), T3 (CEP-3(m)+ASE 1%), T4 (CEP-3 (m)+ASE 2%), T5 (CEP-3(m)+ASE 3%), T6 (CEP-3(m)+ASE 4%), T7 (CEP-3(m)+ASE 5%), and T8 (CEP-3(m)+ASE 6%). The parameters were measured, i.e., the motility of spermatozoa and its kinetic parameters. Data were analyzed using ANOVA with SPSS 16 program. The result showed that the progressive motility of spermatozoa at day one was more than 50% in diluents CEP-3(m)+ASE 1-2%. Diluent CEP-3(m) and aqueous soybean extract 1-2% can support spermatozoa's motility and kinetic parameters during storage at ambient temperature, including progressive motility, motility, motility, velocity, and BCF.
Comparative Study of Feeding and Nutrition for Beef Cattle in Smallholder Farmers: Case Study in Regencies of Malang, Pamekasan, and Tuban: Comparative Study of Feeding and Nutrition for Beef Cattle in Smallholder Farmers: Case Study in Regencies of Malang, Pamekasan, and Tuban Asri Nurul Huda; Kusmartono; Poespitasari Hazanah Ndaru; Trinil Susilawati; Mashudi
Jurnal Ilmu Nutrisi dan Teknologi Pakan Vol. 21 No. 1 (2023): Jurnal Ilmu Nutrisi dan Teknologi Pakan
Publisher : Departemen Ilmu Nutrisi dan Teknologi Pakan Fakultas Peternakan Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jintp.21.1.35-40

Abstract

This study evaluated feeds for fattening and breeding beef cattle in smallholder farmers located in Malang, Pamekasan, and Tuban regencies. The research was conducted using two methods i.e., survey and laboratory analysis. The data survey was carried out in each area, and 30 respondents (n=30) were observed per area in classification of low to medium livestock ownership. Laboratory analysis was performed to analyse the chemical composition of the feed and to measure the total gas production. The data analysis approach used quantitative descriptive to describe the nutritional value quality of beef cattle feed with available quantitative data. The results showed variations in the nutrient content of the beef cattle feed in three different study locations in smallholder farmers located in Regency of Malang, Pamekasan, and Tuban while the total gas production showed a high production in the fattening cattle feeds between three study locations. Thus, it was concluded that the feed for fattening cattle had better nutrient content in terms of crude protein content and total gas production than the breeding cattle feed. Key words: cattle feed, nutrition, gas production
Evaluation The Success of Artificial Insemination Using Frozen Sexed Semen Based on Different Estrus Characters Putri Utami; Muhammad Zainul Hanif; Aulia Puspita Anugra Yekti; Rizki Prafitri; Asri Nurul Huda; Kuswati Kuswati; Kusmartono Kusmartono; Trinil Susilawati
Jurnal Agripet Vol 22, No 2 (2022): Volume 22, No. 2, Oktober 2022
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v22i2.23423

Abstract

ABSTRACT. Knowledge of farmers about estrus detection is one of the factors that affect fixed-time artificial insemination (AI). This study aims to evaluate the success rate of AI using Y sexing frozen semen based on the estrus character of cattle. Forty-five Limousin Crossed Cows involved in this research with a Body Condition Score of 3-5 (1-9 scale), 1.8-7 years old. Y sexing frozen semen is produced by the Singosari Center for Artificial Insemination using Percoll's Gradient Density Centrifugation method. Observation of estrus character was done before insemination with the deep insemination technique of AI. Artificial insemination was carried out using double doses at the 2nd and 8th hours after estrus. Rheinbio vitamins as BioATP+ are injected after AI. The results showed that the Conception Rate and Pregnancy Rate based on the estrus character was higher as indicated by the red colour of the vulva was 17.78% and 22.22%; very swollen vulva was 20% and 22.22%; abundant cervical mucus was 15.56% and 17.78%, and vaginal temperatures ranging from 38.0-38.5oC was 13.33% and 20%. In conclusion, the low percentage of pregnancy in this study was caused by various factors, mainly the genetic quality, early embryonic efficacy, maintenance management especially feed, and there were cows that experienced reproductive disorders.(Evaluasi keberhasilan inseminasi buatan menggunakan semen beku sexing berdasarkan karakter estrus yang berbeda)ABSTRAK. Pengetahuan peternak mengenai deteksi estrus merupakan salah satu faktor yang berpengaruh terhadap ketepatan waktu inseminasi. Penelitian ini bertujuan untuk mengevaluasi tingkat keberhasilan IB menggunakan semen sexing Y berdasarkan karakter estrus ternak. Akseptor yang digunakan pada penelitian ini berjumlah 45 ekor Sapi Persilangan Limousin dengan BCS 3-5 dan umur berkisar antara 1,8-7 tahun. Semen beku sexing Y merupakan hasil produksi Balai Besar Inseminasi Buatan (BBIB) Singosari menggunakan metode Sentrifugasi Densitas Gradien Percoll (SGDP). Pengamatan karakter estrus dilakukan sebelum inseminasi. Inseminasi Buatan dilakukan dengan menggunakan double dosis pada jam ke-2 dan jam ke-8 dengan teknik deep insemination. Vitamin Rheinbio sebagai BioATP+ diinjeksikan setelah IB. Hasil penelitian menunjukkan bahwa keberhasilan kebuntingan ditinjau dari nilai conception rate (CR) dan pregnancy rate (PR) berdasarkan karakter estrus ternak lebih tinggi ditunjukkan pada kondisi warna vulva merah merata yaitu 17,78% dan 22,22%; vulva yang sangat bengkak yaitu 20% dan 22,22%; banyak mengeluarkan lendir servik yaitu 15,56% dan 17,78%, serta suhu vagina yang berkisar antara 38,0-38,5oC yaitu 13,33% dan 20%. Persentase kebuntingan yang rendah pada penelitian ini disebabkan oleh berbagai faktor, utamanya kualitas genetik ternak, kematian embrio dini, manajemen pemeliharaan khususnya pakan, dan terdapat ternak yang mengalami gangguan reproduksi.