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Journal : Journal of Tropical Soils

Isolation of Cellulolytic Bacteria from Peat Soils as Decomposer of Oil Palm Empty Fruit Bunch . Gusmawartati; . Agustian; . Herviyanti; . Jamsari
JOURNAL OF TROPICAL SOILS Vol 22, No 1: January 2017
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2017.v22i1.47-53

Abstract

The aim of the research was to find out potential strainsof cellulolytic bacteria isolated from two tropical peat soils and to studythe potency of the isolated bacteria to decompose oil palm empty fruit bunch (EFB). The research was carried out in two stages: (1) isolation of cellulolytic bacteria from peat soils and (2) testing the potency of isolated bacteria to decompose oil palm EFB. The cellulolytic bacteria were isolated from two peat soils, i.e. a natural peat soil (forest) and a cultivated peat soil (has been used as agriculture land). Isolation of cellulolytic bacteria was conducted by preparing a series dilution of culture solutions using a streak plate method in a carboxymethyl cellulose(CMC) selective medium.Isolates that were able to form clear zones surrounding their bacterial colony were further tested to study the potency of the isolates to decompose cellulose in oil palm EFB. The cellulolytic activity of the selected isolates were further determined via production of reducing sugars in an oil palm EFB liquid medium using Nelson-Somogyi method. The results showed that there are six isolates of cellulolytic bacteria that have been identified in two tropical peat soils used in the current study. Two isolates are identified in a natural peat soil (forest) and four isolates are identified in a cultivated peat soil. The isolates collected are identified as Bacillus sp., Pseudomonassp. and Staphylococcus sp. Among the isolates, an isolate of GS II-1 produces the highest concentration of reducing sugars, namely 0.1012 unitmL-1or 101 ppm, indicating that the isolate of GS II-1 is highly potential to decompose oil palm EFB. Therefore, the isolate of GS II-1 can be used as a decomposer in the bio-conversion processes of oil palm EFB.Keywords: isolation, bacteria, cellulolytic, oil palm empty fruit bunch (EFB), peat soil
Application of Organic Fertilizer Tithonia Plus to Control Iron Toxicity and Reduce Commercial Fertilizer Application on New Paddy Field Hakim, Nurhajati; ., Agustian; Mala, Yanti
JOURNAL OF TROPICAL SOILS Vol. 17 No. 2: May 2012
Publisher : UNIVERSITY OF LAMPUNG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5400/jts.2012.v17i2.135-142

Abstract

Ferrous toxicity is a one of the problems most often found in lowland rice systems in tropical and sub-tropical regionespecially in new paddy field (NPF) on Ultisols and Oxisols. Flooding during rice growth creates a chemicallyreduced state in soils causing reduction of insoluble (Fe3+) to soluble (Fe2+) form even at excessive level. ExcessiveFe2+ uptake is eventually the main cause of iron toxicity. The purpose of this study was to find an appropriateformulation of organic fertilizer Tithonia plus (OFTP) to control the iron toxicity, reduce the application of commercialfertilizers (CF) and increase rice production at various ages of NPF. The field experiment was conducted in farmerfields in Sitiung, Koto Baru sub-district, Dharmasraya district of West Sumatra province. Experimental design usedwas a factorial 2 x 6 in a split plot design. The main plot (A), the age of the opening of NPF were: 0 year;and 2 years;Subplot (B) a formulation of OFTP were: 2 Mg Tithonia ha-1 + 75% of CF; 2 Mg Tithonia ha-1 + 5 Mg rice strawha-1 + 50% of CF; 4 Mg Tithonia ha-1 + 25% of CF and three treatmens without Tithonia i.e. 5 Mg rice straw ha-1 +75% of CF; 2 Mg animal cow manure ha-1 + 75% of CF, and 100% of CF. Liming with 500 kg CaCO3 ha-1were appliedfor all treatments. The results showed the use of OFTP increased the nutrients availability such as N, P, and K onNPF and control the iron toxicity. The appropriate formulation of OFTP to control the iron toxicity and reduced theapplication of chemical fertilizer for higher rice production at NPF were two options: (1) 2 Mg of Tithonia ha-1 + 5 Mgof rice straw ha-1 + 50% of commercial fertilizers; and (2) 2 Mg of Tithonia ha-1 + 75% of commercial fertilizers. In thiscase the reducing of commercial fertilizers application were 50% and 25%, respectively.[How to Cite: Hakim N, Agustian and Y Mala. 2012. Application of Organic Fertilizer Tithonia Plus to Control Iron Toxicity and Reduce Commercial Fertilizer Application on New Paddy Field. J Trop Soils 17: 135-142. Doi: 10.5400/jts.2012.17.2.135][Permalink/DOI: www.dx.doi.org/10.5400/jts.2012.17.2.135]