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Journal : Jurnal Ilmiah Mahasiswa Veteriner

PENGARUH PEMBERIAN VITAMIN E TERHADAP KADAR SUPEROKSIDA DISMUTASE SERUM TIKUS PUTIH (Rattus norvegicus) DIABETES MELITUS (The Effect of Vitamin E to Superoxide Dismutase Serum Level in Diabetes Mellitus Induced White Rat (Rattus norvegicus) ) Regi Putra; dasrul dasrul; sugito sugito; muslim akmal; zuhrawati zuhrawati; syafruddin syafruddin
JURNAL ILMIAH MAHASISWA VETERINER Vol 2, No 2 (2018): FEBRUARI - APRIL
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (245.089 KB) | DOI: 10.21157/jim vet..v2i2.6759

Abstract

ABSTRAKPenelitian ini bertujuan mengetahui pengaruh pemberian vitamin E terhadap kadar SOD serum tikus putih (Rattus norvegicus) diabetes melitus. Penelitian ini menggunakan tikus putih sebanyak 25 ekor dengan kriteria: sehat, bobot badan 150 – 200 gram; umur ± 3 – 4 bulan; dan jenis kelamin jantan. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) pola searah dengan 5 kelompok perlakuan. Masing-masing kelompok perlakuan terdiri atas lima ekor tikus. Kelompok tikus non-diabetes (KN), kelompok tikus diabetes induksi aloksan (KP), kelompok perlakuan yaitu tikus diabetes induksi aloksan dan diberi vitamin E dosis 50 IU/kgbb/hari (P1), 100 IU/kgbb/hari (P2), dan 150 IU/kgbb/hari (P3). Perlakuan vitamin E diberikan selama 28 hari. Pada hari ke-29 setelah perlakuan, dilakukan koleksi serum untuk pengukuran kadar SOD serum dengan spektrofotometer. Data yang diperoleh dianalisis dengan ANOVA satu arah dan uji lanjut Duncan. Hasil penelitian menunjukkan kadar SOD serum adalah 58,00 ± 7,48 U/ml, 36,50 ± 4,56  U/ml (KP), 42,55 ± 5,89 U/ml (P1), 50,44 ± 5,32 U/ml (P2), dan 55,20 ± 4,84 U/ml (P3). Hasil penelitian menunjukkan bahwa vitamin E dapat meningkatan kadar SOD serum secara signifikan (P0,05). Pemberian  vitamin E dengan dosis 150 IU/kg BB/hari menghasilkan peningkatan kadar SOD serum terbaik pada tikus putih diabetes melitus. Kesimpulan dari penelitian ini adalah pemberian vitamin E dapat meningkatkan kadar SOD serum tikus putih diabetes melitus.ABSTRACT            This study aims to know the effect of vitamin E administration to SOD serum level in diabetes mellitus induced white rats (Rattus norvegicus). This study used 25 white rat in criteria: healthy; body weight 150 – 200 gram; age ± 3 – 4 month; and male sex. The research design was used completely randomized design (CRD) with 4 unidirectional pattern groups treatments. Each group consisted of five rat. Group of non-diabetic rats (KN), group of alloxan-induced diabetic rats (KP), group of alloxan-induced diabetic rats which given with vitamin E 50 IU/kgBW/day (P1), 100 IU/kgBW/day (P2), and 150 IU/kgBW/day (P3). Treatment of vitamin E was given in 28 days. On the 29th day after treatment, serum collection was performed to quantify SOD serum level by spectrophotometers. The data obtained were analyzed by one-way ANOVA and continued with Duncan test. The result showed SOD serum level was 58.00 ± 7.48 U/ml, 36.50 ± 4.56 U/ml (KP), 42.55 ± 5.89 U/ml (P1), 50.44 ± 5.32 U/ml (P2), and 55.20 ± 4.84 U/ml (P3). The result showed that therapy of vitamin E could increase SOD serum level significantly (P0.05). Treatment dose of 150 IU/kgBW/day was the best increasing of SOD serum level in diabetes mellitus induced white rat. The conclusion of research showed that giving of vitamin E can increasing the SOD serum level in diabetes mellitus induced white rat.
Prediksi Kadar Protein dan Lemak Daging Sapi Aceh Menggunakan Aplikasi Near Infrared Reflectunce Spectroscopy (NIRS) Nirma Rotua; Teuku Reza Ferasyi; Cut Dahlia Iskandar; Zuhrawati Zuhrawati; Herrialfian Herrialfian; Teuku Zahrial Helmi
JURNAL ILMIAH MAHASISWA VETERINER Vol 1, No 4 (2017): AGUSTUS-OKTOBER
Publisher : JURNAL ILMIAH MAHASISWA VETERINER

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (274.826 KB) | DOI: 10.21157/jim vet..v1i4.4773

Abstract

Tujuan penelitian ini adalah untuk mengetahui  kemampuan teknologi NIRS guna memprediksi kadar lemak dan protein daging sapi aceh. Penentuan kadar protein dan lemak daging sapi aceh dilakukan pada regio Longissimus dorsi. Sampel diperoleh dari pasar Peunayong dan Lambaro, meliputi 2 sampel daging dengan masing-masing daging 3 kali pengulangan. Penelitian ini  menggunakan metode Principal Component Analysis (PCA) untuk menentukan kandungan protein dan lemak daging sapi aceh  Hasil penelitian ini menunjukan  bahwa nilai aktual laboratorium dengan nilai prediksi NIRS memperoleh nilai yang akurat ditunjukkan dengan masing-masing R-square prediksi kadar protein dan lemak daging sapi aceh 0,99 dan 0,99 yaitu variabel prediksi terbaik. Kesimpulan dari penelitian ini adalah NIRS mampu memprediksi kadar protein dan lemak daging sapi aceh dengan sangat baik karena diperoleh nilai aktual dengan nilai prediksi dan metode NIRS dapat memprediksi kadar protein dan lemak daging sapi aceh secara akurat, karena diperoleh nilai R² = 0,99.This study aims to know the ability of NIRS technology to predicted  of fat  and protein content of aceh beef. Determination of protein and  fat content of aceh beef  was done in the Longissimus dorsi region. This research used Principal Component Analysis (PCA) method to determine protein  and  fat content of aceh beef. Samples were obtained from Peunayong and Lambaro markets. Sampled beef consisted of beef 2 samples with 3 repetition. The results of this study showed that the actual value of the laboratory with a predicted value of NIRS obtained an accurate value indicated by each R-square prediction protein  and fat content of aceh beef aceh  of 0.99 and 0.99 was the best predictive variable. It can be concluded that NIRS was able to predict the levels of protein and fat of beef as accurately. The conclusion of this study were that NIRS was able to predicted the protein and fat content of aceh beef very well because it was obtained by the actual value with prediction value and the NIRS method can predict the protein and fat content of beef aceh accurately, because the value of  R² = 0,99.