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Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.

Application of Spa-Gene as A Marker of Molecular Epidemiology in the Case of Methicillin-Resistant Staphylococcus Aureus (MRSA) Which its from Dairy Cows in Surabaya Region Asri Rizky; Mustofa Helmi Effendi; Jola Rahmahani
Jurnal Kedokteran Hewan Vol 15, No 2 (2021): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i2.4817

The aim of this study was to isolate and identify the strain of methicillin-resistant Staphylococcus aureus (MRSA) from cow’s milk in Surabaya and to determine the fragment Spa-Gene in MRSA strains. There were 50 samples of cow’s milk obtained from four dairy farms. From 50 tested samples, 19 samples (38%) were positive for Staphylococcus aureus. Antibiotic sensitivity test using oxacillin and erythromycin showed that 6 samples were resistant to the antibiotic oxacillin and 6 samples were resistant to erythromycin. MRSA confirmation tests that were conducted on 6 samples revealed 5 positive results for the MRSA strain. Electrophoresis of Polymerase Chain Reaction (PCR) product showed that 4 out of 5 samples were positive for the presence of the Spa-Gene fragment. The research results showed that there were 3 models of Spa-Gene fragments; the first had a length of 90 bp and 140 bp, the second had a length of 140 bp, and third had a length of 90 bp. This study revealed the nature of Spa-Gene polymorphism of MRSA strains isolated from milk samples. It was concluded that the Spa-Gene can be used as a molecular epidemiological marker of the MRSA strain.

PENGARUH PEMBERIAN INFUSA DAUN LABU SIAM (Sechium edule) TERHADAP KADAR HEMOGLOBIN DAN NILAI HEMATOKRIT TIKUS PUTIH (Rattus norvegicus) ANEMIA (The Effect of Chayote (Sechium edule) Leaves Infusion on Haemoglobin and Hematocrit Level of Anemic White Rat (Rattus norvegicus)) Zuhrawati Zuhrawati; Nuzul Asmilia; Asri Rizky; Zuraidawati Zuraidawati; Nazaruddin Nazaruddin; Mulyadi Adam; Muttaqien Muttaqien
Jurnal Medika Veterinaria Vol 9, No 2 (2015): J. Med. Vet.
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.med.vet..v9i2.3945

The aim of this research was to evaluate the effect of chayote (Sechium edule) leaves infusion on hemoglobin and hematocrit level of anemic rat (Rattus norvegicus). Twelve anemic hemorrhagic male rats strain Wistar with body weight ranging from 175-200 g were used as sample in this research. Rats were divided randomly into 3 treatment groups, each group consist of 4 rats. The control group (P0) was not given chayote leaves infusion. Group P1 and P2 were given chayote leaves infusion with the concentration of 25% and 50% respectively, with dose of 1 ml per 200 gram body weight. Rats were fed with standard commercial food (Turbo feed) and aquadest ad libitum. The rats were treated anemic by taking 20% blood from total blood volume prior to administration of chayote laeves infusion. Chayote leaves infusion was given for 10 days consecutively. Blood sampling was carried out on day 0, 5, and 10 via caudal vein. Data were analyzed using anlysis of variance (ANOVA) Split-Plot pattern. The result showed that chayote leaves infusion increase level of hemoglobin and hematocrit in experimental rats. In conclusion 25% concentration of chayote leaves infusion is more effective than 50% concentration.Key words: anemia, chayote leaves, hemoglobin, hematocrit

Management of Digit Papillomas in Golden Retriever Dogs Jessica Anggun Safitri; Sarwo Edy Wibowo; Ahmad Syarifuddin; Dedi Damhuri; Puti Puspa Seruni; Asri Rizky
Journal of Applied Veterinary Science And Technology Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/javest.V6.I1.2025.76-80

Background: Papillomas are benign tumors that affect the skin or soft tissue beneath the skin. These papillomas can be accompanied by various abnormalities such as pustules, itching, hematomas, cysts, blisters, abscesses, lick granulomas, and skintags. While papillomas may resolve on their own, some cases require intensive treatment and management. Purpose: To provide veterinarians with insights into diagnosing and managing papilloma cases. Case: A 10-year-old male Golden Retriever presented with an open wound on the left front paw nail. The wound originated from a broken nail, which led to a non-healing wound over a period of 2 months. Clinical examination revealed a mass-like lesion that had ruptured. Palpation of the left front paw nail area indicated an irregular, lobular growth resembling grapes or cauliflower. Case Management: Routine hematology revealed that the dog had microcytic hypochromic anemia, while blood chemistry indicated mildly reduced kidney function. Cytology confirmed that the wound was a papilloma. Treatment involved digit amputation to completely remove the tumor mass, along with wound care that included the application of ointments, antibiotics, analgesics, hematopoietic agents, anti-bleeding medication, and anti-cancer drugs. Supportive treatment included multivitamins, nerve supplements, immune supplements, and skin and coat supplements (Coatex). By the fifth postoperative day, the wound had dried, and the edges were healing well. Conclusion: Papillomas are benign and not harmful, but it is recommended to completely remove the tumor mass to prevent secondary infections.

Management of chronic kidney disease in a young adult-aged male domestic cat Rizky , Asri; Decline , Vega; Viastika , Yanita Mutiaraning; Tjahajati , Ida; Rahayu , Pudji; Wardani , Sundika; Wibowo , Sarwo Edy
ARSHI Veterinary Letters Vol. 9 No. 1 (2025): ARSHI Veterinary Letters - February 2025
Publisher : School of Veterinary Medicine and Biomedical Sciences, Bogor Agricultural University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avl.9.1.11-12

Chronic Kidney Disease (CKD) is an irreversible condition resulting in steady renal function deterioration. This decline impedes the ability of the body to regulate metabolism, fluids, and electrolytes. This paper reports the case of a young adult-aged male domestic cat, named Kuning, who was brought to the clinic with severe symptoms, including hematemesis, anorexia, and dehydration, and received intravenous fluid therapy via a 24G catheter. On the third day of hospitalization, ultrasonography revealed significant kidney abnormalities, including irregular cortical surfaces, indistinct cortico-medullary boundaries, focal hyperechoic areas in the cortex, and medullary ring sign. The urinary bladder appeared intact with an anechoic lumen. Liver ultrasonography revealed blunted edges in the right and left lobes and focal hyperechoic diffuse changes in the parenchyma, suggesting hepatic involvement. Following intensive treatment, Kuning's condition improved. Routine hematological tests on days 9 and 16-23 showed positive trends, with increased platelet counts and elevated leukocyte levels. Liver function improved compared to the initial assessments but remained outside the normal range. Based on anamnesis, clinical examination, and diagnostic findings, Kuning was diagnosed with CKD. After 27 days of intensive hospitalization, Kuning exhibited steady recovery, highlighting the importance of early diagnosis and comprehensive management in CKD cases.

MANAGEMENT OF BULBUS OCULI PROLAPSE IN A CAT AT JOGJA VETERINARY CLINIC, YOGYAKARTA Decline, Vega; Rizky, Asri; Viastika, Yanita Mutiaraning; Seruni, Puti Puspa; Wibowo, Sarwo Edy
VITEK : Bidang Kedokteran Hewan Vol 15 No 1 (2025): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v15i1.320

Enucleation is an orbital surgical procedure performed on patients with indications of increased intraocular pressure due to glaucoma that cannot be treated with medication, intraocular neoplasia that may cause intraocular pain, or severe trauma. Prolapse of the bulbus oculi in cats is generally caused by glaucoma (increased intraocular pressure), bacterial or viral infections, trauma from being hit by a vehicle, scratches, fights with other animals, or ocular tumors. The purpose of this report is to describe the management of enucleation in cats at Jogja Veterinary Clinic, Yogyakarta. The subject of this case is a female cat named Telon, approximately four months old, weighing 1.65 kg, who was brought to Jogja Veterinary Clinic with a complaint of a protruding eye. Based on anamnesis, physical examination, and clinical symptoms, Telon was diagnosed with bulbus oculi prolapse and underwent enucleation surgery as a medical intervention. The treatment provided included antibiotics, vitamins, analgesics, and topical ointment. The cat received postoperative care for four days and showed good wound healing. It was concluded that the enucleation procedure using the transpalpebral technique in Telon had a favorable prognosis. Keywords: Prolapse of the Bulbus Oculi, Cats, and Enucleation

CLINICAL APPROACH TO ORAL PAPILLOMATOSIS IN CATS: A CASE STUDY Viastika, Yanita Mutiaraning; Decline, Vega; Rizky, Asri; Pratiwi, Retno Anggie; Wibowo, Sarwo Edy
VITEK : Bidang Kedokteran Hewan Vol 14 No 2 (2024): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v14i2.297

Papilloma refers to the abnormal growth of tissue in the form of tumors or warts. The clinical signs of oral papillomatosis include lesions or masses that develop inside the oral cavity. Oral papillomatosis in cats is caused by Canine Papillomavirus (CPV). The papillomavirus induces tumors by infecting cells through wounds. The purpose of this report is to describe the diagnosis and management of oral papillomatosis in a mixed domestic cat at the Yogyakarta Animal Clinic. Diagnosis of papillomatosis can be made based on visible clinical signs and confirmed through cytological examination of the tumor mass. Management of oral papillomatosis involves the surgical removal of the tumor mass along with the surrounding tissue. Postoperative therapy includes the administration of antibiotics (Cefixime®), anti-inflammatory drugs (Methylprednisolone®), and antifungal agents (Candistatin®).

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