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Identifikasi Serovar Penyebab Leptospirosis pada Anjing di Yogyakarta Guntari Titik Mulyani; Sri Hartati; Hastari Wuryastuty; Ida Tjahajati; Yuriadi Yuriadi; Irkham Widiyono; Yanuartono Yanuartono; Hary Purnamaningsih; Soedarmanto Indarjulianto; Slamet Raharjo; Alfariza Nururozi; Angeline Ganapragasam; Yeo Suan Jiao
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.014 KB) | DOI: 10.22146/jsv.39201

Abstract

Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
Faktor Risiko Feline Panleukopenia pada Kucing di Daerah Istimewa Yogyakarta Riyandini Putri; Bambang Sumiarto; Guntari Titik Mulyani
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.48922

Abstract

AbstractFeline panleukopenia virus (FPV) infection in cats caused a disease called feline panleukopenia with high morbidity and mortality worldwide. There is no causative therapy for this disease yet, therefore knowing risk factors is important measure to prevent the disease. This study was designed to investigate the risk factors of feline panleukopenia in Special Region of Yogyakarta therefore disease prevention can be done as early as possible. A retrospective case-control study that used 35 cats in the case group and 70 cats in the control group. Case group consisted all feline panleukopenia patients that were tested positive with Anigen FPV Ag Test Kit (Bionote, South Korea) from two local clinics. Control group consisted cats that were own by the neighbour of cats from the case group with age and sex as matching variables. Owner characteristics including formal education, occupation, duration of having cat(s), monthly maintenance cost, and interaction time per day also feline characteristics including breed, neuter status, origin, husbandry, and vaccination status were assessed. Association analysis was done using EpiInfo (chi-square and odds ratio) and manually calculated (estimated attributable fraction and estimated population attributable fraction). Associated risk factors of feline panleukopenia were duration of having cat(s) less than a year, domestic cat breed, cat origins such as pet store or market and stray cat, the presence of new cat(s), outdoor cat lifestyle, waste disposal outdoor, and not vaccinated cat. Vaccination status and the presence of new cat(s) had the highest strength compared to other risk factors. It can be concluded that vaccination and quarantine of new animals are important measures to prevent feline panleukopenia.Keywords: case-control study, cats, feline panleukopenia
Akumulasi Fibrin dalam Anterior Chamber Pada Kucing Penderita Tripanosomiasis dan Feline Immunodeficiency Virus Kurnia Kurnia; Dyah Kunthi Wirapratiwi; Setyo Budhi; Guntari Titik Mulyani; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54614

Abstract

Tripanomiasis merupakan penyakit yang disebabkan oleh Tripanosoma sp, protozoa hemoflagellata dari kelas Zoomatigophora dan famili Tripanosomatidae. Tripanosomiasis banyak dijumpai di daerah tropis dan menyerang berbagai hewan domestik seperti kuda, sapi, kerbau, onta, anjing, kucing dan tikus. Feline immunodeficiency virus (FIV) dikenal sebagai  feline AIDS adalah spesies virus dalam genus Lentivirus, menyebabkan penurunan sistem imun pada kucing dimana tubuh tidak dapat mengatasi serangan dari berbagai sumber penyakit lain sehingga muncul infeksi tambahan. Umumnya kucing tidak menimbulkan gejala klinis infeksi FIV meskipun telah berlangsung beberapa tahun. Seekor kucing domestik jantan, 3 tahun didiagnosis Tripanosomiasis dan positif FIV. Kedua mata terlihat berwarna keputihan, berawal dari mata kiri, berukuran kecil yang berkembang secara progresif selama 2 minggu, diikuti penurunan nafsu makan serta kondisi badan yang semakin kurus. Kucing berasal dari kucing jalanan, dan gemar memakan tikus maupun burung, serta belum dilakukan vaksinasi. Hasil pemeriksaan klinis menunjukkan mukosa anemik, dehidrasi, oedema di daerah submandibular hingga bahu, BCS 4/9, dan uveitis anterior. Hasil pemeriksaan hematologi dan kimia darah menunjukkan anemia normositik-hipokromik, trombositophenia, normal leukosit total dengan peningkatan relatif monosit, nilai SGPT dan SGOT yang sangat tinggi, peningkatan creatinin dan penurunan total kolesterol. Hasil pemeriksaan rapid test menunjukkan positif antibodi FIV (Feline Immunodeficiency Virus), negatif Feline Leukemia Virus, serta negatif  toksoplasma. Pemeriksaan apus darah menunjukkan mild anemia tanpa polikromasia, dan ditemukan flagellata Trypanosoma sp. Akumulasi fibrin di dalam anterior chamber yang bersifat progresif-bilateral disertai dengan aqueous flare dan normal retina merupakan gambaran anterior uveitis sebagai gejala klinis yang menciri dari Tripanosomiasis pada kucing dan infeksi FIV.
STATUS KLINIS DAN DETEKSI LipL32 SAPI SEROPOSITIF LEPTOSPIROSIS DI KABUPATEN KULON PROGO Guntari Titik Mulyani; Wayan Tunas Artama; Estu Widodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55930

Abstract

  Leptospirosis is a zoonotic disease caused by Leptospira interrogans. Animals can act as carriers, spread leptospires in urine, and be a source of infection for other animals and humans. In leptospirosis cows can cause abortion, early birth, infertility, decreased milk production and death. The aims of this study was to determine the clinical status and detect the presence of leptospires with Polymerase Chain Reaction (PCR) from urine cows that are expressed as leptospirosis seropositive. A total of 12 cattle seropositive leptospirosis with Microscopic Agglutination Test (MAT) were carried out clinical examinations covering general conditions, pulsus examination, breathing and temperature and organ systems. The urine is collected aseptically, then DNA isolation is carried out using a kit from Genoid. Detection of leptospires in the urine is carried out by detecting the presence of the primary lipoprotein LipL32 making up the Leptospira membrane. The primer was designed with a 21-base forward forward: 5'-TGG ATC TGA TCA ACT ATT ACG-3 ‘containing 38.1% GC with Tm 57.2oC. 22 bases reverse reverse obtained: 5 '-CAC TTC ACC TGG TTT GTA GGT-3' containing GC 45.5% with Tm 62.1oC. Amplification was carried out as many as 40 cycles and continued with electrophoresis to determine the band formed at a wavelength of 506 bp. The results showed that all cows that were positive for leptospirosis with MAT were in a clinically healthy condition. In electrophoresis there are 7 out of 12 positive samples found in the urine leptospira indicated by the formation of a band at 506 bp. From the results of this study it can be concluded that leptospirosis-positive cows do not always show clinical symptoms, but have the potential to excrete leptospires along with urine, so they can act as a source of transmission of leptospirosis to humans, other animals, and the environment. 
Efficacy of Albendazole Against Strongylus sp. and Hematology Changes on Equine in Yogyakarta Special Region yuriadi yuriadi; Ida Tjahajati; Guntari Titik Mulyani; Kelviano Muqit; Aidah Rahmanita
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60110

Abstract

The infestation of Strongylus sp. in horses can cause losses to horse breeders, including anorexia, anemia, gastrointestinal diseases and can cause death and decrease the horse population in DIY. Albendazole was a Benzimidazole preparation that is often used to treat worms in ruminants. This study also aims to determine the effect of Albendazole on blood images before and after treatment. The material used in this study were 10 horses with male and female sex,  over 3 years old, and infected with Strongylus sp. with an infestation rate of 200 EPG in faeces. Before treatment of drug was carried out, the faeces was examined with Mc Master method. After treatment with Albendazole, the worm eggs were examined three times at intervals of three days. Routine examination of worm eggs and blood was carried out at the Laboratory of the Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada. The results showed that the EPG number decreased from the 0th, 3rd, 6th and 9th day of examinations. The average number of EPG on the 0th day was 990 eggs/gram, and the examination on the 9th day showed the number of eggs was 0 eggs/gram. The results of the hematology examination also showed no significant difference except for the high number of eosinophils on the 12th day after drug administration. The conclusion from this research is that Albendazole as a worm medicine can kill Srongylus sp. however, it does not affect the horse's hematology and health status.
Identifikasi Tipe Dehidrasi dan Profil Elektrolit Mayor pada Pasien Kucing di Rumah Sakit Hewan Prof. Soeparwi dan Beberapa Klinik Hewan di Wilayah Yogyakarta Guntari Titik Mulyani; Setyo Budhi; Kurnia .
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69901

Abstract

Dehydration is defined as a lack of body fluids followed by loss of electrolytes, and changes in acid-base balance. The type of dehydration is limited based on the concentration of sodium in serum at the time of dehydration. Maintenance of osmotic pressure and distribution of several compartments of body fluids are the main functions of the four major electrolytes, namely sodium (Na +), potassium (K +), chloride (Cl‾), and bicarbonate (HCO3‾). Sodium is the most cation in extracellular fluid, most potassium cation in intracellular fluid and chloride is the most anion in extracellular fluid. The purpose of this study was to identify the type of dehydration and to determine the major electrolyte profile in cats in Yogyakarta and its surroundings. This study used 18 sick cats that were thought to be dehydrated, marked by decreased skin turgor, CRT> 2 seconds, and 12 cats that were suspected of having electrolyte balance disorders with symptoms of ascites, uropoetic disorders. Blood was drawn for all cats to measure Pack Cells Volume (PCV) levels. Patient clinical data and patient diagnosis were recorded, cats with changes in serum PCV levels were separated for examination of levels of sodium, chloride, potassium using Seamaty SMT-120V. The type of dehydration is identified based on the sodium level in the serum of a dehydrated cat. The results showed that most of the cat patients were dehydrated had low serum sodium levels (hyponatremia). There was 1 cat patient had low chloride levels. Potassium levels in cats with UT obstruction increased, which led to a decrease in the Na: K ratio. Cat bicarbonate levels did not show any change. From the results of the study it was concluded that dehydration in cats at Prof. Soeparwi is hypotonic dehydration (71%). The sodium profile mostly decreased, chloride and bicarbonate levels did not change, while there were changes in potassium levels in patients with UT disorders. The advice given is to check electrolytes before doing fluid therapy. Prior to electrolyte testing, dehydrated cats can be given a sodium solution. 
Sosisalisasi Kesehatan Hewan Kepada Masyarakat Secara Daring: Online Socialization of Animal Health to The Community Soedarmanto Indarjulianto; Yanuartono Yanuartono; Slamet Raharjo; Alfarisa Nururrozi; Hastari Wuryastuty; Irkham Widiyono; Hary Purnamaningsih; Guntari Titik Mulyani; Ida Tjahajati; Sri Hartati; Yuriadi Yuriadi
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2021): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v2i2.223

Abstract

ABSTRACT Various animal diseases are not always known by animal owners, so they must always be socialized even in the condition of the COVID-19 outbreak. This community service aims to provide animal health information to the community by online. This activity was carried out from in November 2020 using the Cisco Webex platform. The results showed that as many as 269 participants have joined in the socialization, consisting of 180 from Yogyakarta and 89 from outside Yogyakarta. Concluded that online socialization of animal health is successfully carried out and received a very good response from the community. Keywords: Animal health; Community service; Veterinary clinic ABSTRAK Berbagai macam penyakit hewan tidak selalu diketahui oleh pemilik hewan, sehingga harus selalu disosialisasikan walapun dalam kondisi wabah COVID-19. Pengabdian kepada masyarakat (PKM) ini bertujuan memberikan informasi kesehatan hewan kepada masyarkat secara daring. Pengabdian dilakukan pada bulan bulan Oktober sampai Nopember 2020 secara daring menggunakan platform Cisco Webex. Hasil PKM menunjukkan bahwa sebanyak 269 calon peserta telah mengikuti sosialisasi, yang terdiri dari 180 berasal dari Yogyakarta dan 89 dari luar Yogyakarta. Berdasar hasil kegiatan ini, disimpulkan bahwa sosialisasi tentang kesehatan hewan secara daring berhasil dilaksanakan dan mendapat respon sangat bagus dari masyarakat. Kata kunci: Kesehatan hewan; Klinik hewan; Pengabdian masyarakat.
Pengembangan Basis Data Sistem Informasi Manajemen Rumah Sakit Berbasis Linguistic-based Schema Matching Adhistya Erna Permanasari; Hayu Pradnya Satyaprabha; Addin Suwastono; Guntari Titik Mulyani
Jurnal Nasional Teknik Elektro dan Teknologi Informasi Vol 8 No 2: Mei 2019
Publisher : Departemen Teknik Elektro dan Teknologi Informasi, Fakultas Teknik, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (958.789 KB)

Abstract

Prof. Soeparwi veterinary hospital is one of the medical institutions focused on providing medical services for animals. In its daily operations, the flow of informations among various departments has yet to be supported by management information systems (MIS) which enables efficiency of business process and a better management of the data. Previous researches have been conducted to develop MIS, which resulted in three independent MIS for managing registration, medical records, and patient bills. Each MIS is using their own database to store the data, thus causing duplicates of information and inconsistencies, and also increasing complexity in accessing the data. The goal of this research is to re-design the said database into a single database that will be used by various MIS. Three independent databases are merged by applying a technique that uses linguistic information as the basis for the matching–called linguistic-based schema matching. This method’s accuracy is evaluated by calculating precision, recall, and F-measure–which we obtained scores of above 50% for all three indicators. Requirement analysis is performed to further develop the database for supporting further needs of the hospital. The new database system is tested using black-box technique under few test cases to see if its functionality corresponds with the specifications defined. Result of this test proves that the new database could handle valid, invalid, and redundant inputs as expected by a score of 100% success rate.
Pemeriksaan kesehatan kucing di Yogyakarta: Health Examination of Cat in Yogyakarta Soedarmanto Indarjulianto; Hastari Wuryastuty; Slamet Raharjo; Hary Purnamaningsih; Guntari Titik Mulyani; Ida Tjahajati
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 4 No. 1 (2023): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v4i1.337

Abstract

Cats are pets that many people, including in Yogyakarta, are keeping. Various kinds of diseases can attack cats, but not all owners are able to check their cat's health status. This community service aims to carry out health checks for cats owned by the community especially animal owners who are customers of the Veterinary Clinic of Internal Medicine, FVM-UGM. The service was carried out from April to October 2022 by checking the cat's health status directly. The service has been conducted and can carry out examinations and therapy of 20 cats. Based on the examination, it was found that 17 cats (85%) were diagnosed as sick and three healthy cats (15%). The three main diseases found were skin, digestive and urinary systems. Through medical examinations by students accompanied by veterinarians in the Department of Internal Medicine, cat owners have been invited to watch and given information regarding disease diagnosis, therapy, and how to administer medicine. The owner already understands the cat’s health status, agree to the action and treatment and can provide medicine for the sick cat. It was concluded that the community service for cat health examination had been successfully carried out on 20 cats, and the owner knew the condition of the cat and was able to give the medicine given. Key words: Cat Examination; Community Service; Veterinary Clinic
KAJIAN LEPTOSPIROSIS PADA SAPI POTONG DI DAERAH ALIRAN SUNGAI PROGO DAERAH ISTIMEWA YOGYAKARTA (The Study of Bovine Leptospirosis in Progo Watershed, Yogyakarta) Guntari Titik Mulyani; Bambang Sumiarto; Wayan Tunas Artama; Sri Hartati; Juwari J; Sugiwinarsih S; Henricus Roby Cahya Putra; Estu Widodo
Jurnal Kedokteran Hewan Vol 10, No 1 (2016): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (302.098 KB) | DOI: 10.21157/j.ked.hewan.v10i1.3374

Abstract

The purpose of this study was to determine the prevalence, serovar, and risk factors of leptospirosis on cattle in Progo watershed. A total of 330 cattle samples were clinically examined and blood was collected for Leptospira examination using microscopic agglutination test (MAT). Results of MAT were used as dependent variable (Y), while breeders, cattle, shed, and feed factors were used as the independent variable (X). Univariate analysis, bivariate analysis with chi square, odds ratio, and multivariate with logistic regression were implemented to analyze the data. The results showed that all cattle were cinically health, 13.03% (43/330) cattle were positively leptospirosis which 37 out of 193 (9.17%) cattle belongs to the farmer. Mostly, leptospirosis cases were caused by hardjo serovar (38.0%). Risk factors influencing the prevalence of leptospirosis were caused by some factors such as the presence of rats in cage (OR 2.7), the distance of waste disposal which should be less than approximately 5 meters (OR 2.1), feed concentrates, grass and rice straw (OR 0.4), grazing (OR 0.2), and concentrate feed and rice straw (OR 0.1). It can be concluded that leptospirosis infection on cattle in Progo watershed is 13.03% (at livestock level) and 19.17% (at farm levels). Factors of rat in the cage and the distance of waste disposal (5 m) increase the risk of leptospirosis cases. Factors of feed combine with concentrate and rice straw as well as grazing reduced the risk of leptospirosis in cattle, but the addition of grass in concentrate feed and rice straw provides a greater risk of leptospirosis infection compare to concentrate feed and rice straw alone.Key words: beef cattle, leptospirosis, prevalence, risk factors
Co-Authors Addin Suwastono, Addin Adhistya Erna Permanasari Agung Budi Pramono Agung Budi Purnomo Agustin Indrawati Aidah Rahmanita Alfarisa Nururrozi Alfarisa Nururrozi, Alfarisa Alfariza Nururozi Ambar Widuri Andriani Dwi Hapsari Angeline Ganapragasam Anis Pramundari Antoni Kirwanto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Bambang Sumiarto Datrianto, Dwi S. Dwi Priyowidodo Dyah Kunthi Wirapratiwi Dyah Kunti Wirapratiwi Dyah Kunti Wirapratiwi Eko Sulistyadi Estu Widodo Estu Widodo Estu Widodo, Estu Hary Purnamaningsih Hary Purnamaningsih Haryadi Haryadi Hastari Wuryastuti Hastari Wuryastuti Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Hastari Wuryastuty Hayu Pradnya Satyaprabha Henricus Roby Cahya Putra Henricus Roby Cahya Putra, Henricus Roby Cahya Ida Tjahajati Irkham Widiyono Juwari J Juwari J, Juwari Kelviano Muqit Kurnia . Kurnia Kurnia Kurnia Kurnia Kurnia Kurnia Laksono Trisnantoro Martathama, Gadis Pramono, Agung Budi Retno Widyastuti, Retno Riyandini Putri Satyaprabha, Hayu Pradnya Setyo Budhi Setyo Budhi Setyo Budhi Slamet Raharjo Slamet Raharjo Slamet Raharjo Slamet Raharjo Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Soedarmanto Indarjulianto Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sri Hartati Sugiwinarsih S Sugiwinarsih S, Sugiwinarsih Teguh Budipitojo Tri Atmojo Tri Untari Wayan Tunas Artama Yanuartono . Yanuartono Yanuartono Yanuartono Yanuartono Yanuartono, Yanuartono Yeo Suan Jiao Yuda Heru Fibrianto Yuda Heru Fibrianto Yuli Santoso Yuli Santoso Yuriadi . Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi Yuriadi, Yuriadi Yuriati .