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Identifikasi Serovar Penyebab Leptospirosis pada Anjing di Yogyakarta Guntari Titik Mulyani; Sri Hartati; Hastari Wuryastuty; Ida Tjahajati; Yuriadi Yuriadi; Irkham Widiyono; Yanuartono Yanuartono; Hary Purnamaningsih; Soedarmanto Indarjulianto; Slamet Raharjo; Alfariza Nururozi; Angeline Ganapragasam; Yeo Suan Jiao
Jurnal Sain Veteriner Vol 37, No 2 (2019): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.014 KB) | DOI: 10.22146/jsv.39201

Abstract

Leptospirosis is a zoonotic disease of global concern, and is caused by pathogenic serovar Leptospira interrogans. Canine Leptospirososis is widespread worldwide, dogs can act as incidental hosts or maintenance hosts for various serovars. The purpose of this research was to identify leptospire serovars that infect healthy and suspected leptospirosis dogs in Yogyakarta. A total of 56 dogs (36 healthy dogs and 20 suspect leptospirosis dogs) sera were taken from cephalica vein as much as 3 ml. Sera were examined for leptospirosis with Microscopic Aglutination Test (MAT) which conducted at the Research Center for Veterinary Science, Bogor. Microscopic Aglutination Test carried out on various Leptospire serovar, namely: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, and Tarrasovi. The results showed that Celledoni serovars infected 25% of healthy dogs and 5% of suspect leptospirosis dogs, Javanica serovar infected 19% of healthy dogs, Bataviae serovars infected 15% of suspect leptospirosis dogs, Grippotyphosa serovar infected 11% of healthy dogs, Tarrasovi serovar infected 10% of suspect leptospirosis dogs, serovars Cynopteri infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, serovar Pyrogenes infects 5% of healthy dogs and 5% of suspect leptospirosis dogs, and serovar Rachmati infects 5% of suspect leptospirosis dogs. Seven healthy dogs (19%) and 2 suspect leptospirosis dogs (10%) were infected with more than 2 leptospire serovars. From the results of this study it can be concluded that Celledoni serovar of Leptospira interrogans infection causes subclinical leptospirosis, while Bataviae serovar infection causes clinical leptospirosis in dogs in Yogyakarta.
Faktor Risiko Feline Panleukopenia pada Kucing di Daerah Istimewa Yogyakarta Riyandini Putri; Bambang Sumiarto; Guntari Titik Mulyani
Jurnal Sain Veteriner Vol 38, No 3 (2020): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.48922

Abstract

AbstractFeline panleukopenia virus (FPV) infection in cats caused a disease called feline panleukopenia with high morbidity and mortality worldwide. There is no causative therapy for this disease yet, therefore knowing risk factors is important measure to prevent the disease. This study was designed to investigate the risk factors of feline panleukopenia in Special Region of Yogyakarta therefore disease prevention can be done as early as possible. A retrospective case-control study that used 35 cats in the case group and 70 cats in the control group. Case group consisted all feline panleukopenia patients that were tested positive with Anigen FPV Ag Test Kit (Bionote, South Korea) from two local clinics. Control group consisted cats that were own by the neighbour of cats from the case group with age and sex as matching variables. Owner characteristics including formal education, occupation, duration of having cat(s), monthly maintenance cost, and interaction time per day also feline characteristics including breed, neuter status, origin, husbandry, and vaccination status were assessed. Association analysis was done using EpiInfo (chi-square and odds ratio) and manually calculated (estimated attributable fraction and estimated population attributable fraction). Associated risk factors of feline panleukopenia were duration of having cat(s) less than a year, domestic cat breed, cat origins such as pet store or market and stray cat, the presence of new cat(s), outdoor cat lifestyle, waste disposal outdoor, and not vaccinated cat. Vaccination status and the presence of new cat(s) had the highest strength compared to other risk factors. It can be concluded that vaccination and quarantine of new animals are important measures to prevent feline panleukopenia.Keywords: case-control study, cats, feline panleukopenia
Akumulasi Fibrin dalam Anterior Chamber Pada Kucing Penderita Tripanosomiasis dan Feline Immunodeficiency Virus Kurnia Kurnia; Dyah Kunthi Wirapratiwi; Setyo Budhi; Guntari Titik Mulyani; Dwi Priyowidodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.54614

Abstract

Tripanomiasis merupakan penyakit yang disebabkan oleh Tripanosoma sp, protozoa hemoflagellata dari kelas Zoomatigophora dan famili Tripanosomatidae. Tripanosomiasis banyak dijumpai di daerah tropis dan menyerang berbagai hewan domestik seperti kuda, sapi, kerbau, onta, anjing, kucing dan tikus. Feline immunodeficiency virus (FIV) dikenal sebagai  feline AIDS adalah spesies virus dalam genus Lentivirus, menyebabkan penurunan sistem imun pada kucing dimana tubuh tidak dapat mengatasi serangan dari berbagai sumber penyakit lain sehingga muncul infeksi tambahan. Umumnya kucing tidak menimbulkan gejala klinis infeksi FIV meskipun telah berlangsung beberapa tahun. Seekor kucing domestik jantan, 3 tahun didiagnosis Tripanosomiasis dan positif FIV. Kedua mata terlihat berwarna keputihan, berawal dari mata kiri, berukuran kecil yang berkembang secara progresif selama 2 minggu, diikuti penurunan nafsu makan serta kondisi badan yang semakin kurus. Kucing berasal dari kucing jalanan, dan gemar memakan tikus maupun burung, serta belum dilakukan vaksinasi. Hasil pemeriksaan klinis menunjukkan mukosa anemik, dehidrasi, oedema di daerah submandibular hingga bahu, BCS 4/9, dan uveitis anterior. Hasil pemeriksaan hematologi dan kimia darah menunjukkan anemia normositik-hipokromik, trombositophenia, normal leukosit total dengan peningkatan relatif monosit, nilai SGPT dan SGOT yang sangat tinggi, peningkatan creatinin dan penurunan total kolesterol. Hasil pemeriksaan rapid test menunjukkan positif antibodi FIV (Feline Immunodeficiency Virus), negatif Feline Leukemia Virus, serta negatif  toksoplasma. Pemeriksaan apus darah menunjukkan mild anemia tanpa polikromasia, dan ditemukan flagellata Trypanosoma sp. Akumulasi fibrin di dalam anterior chamber yang bersifat progresif-bilateral disertai dengan aqueous flare dan normal retina merupakan gambaran anterior uveitis sebagai gejala klinis yang menciri dari Tripanosomiasis pada kucing dan infeksi FIV.
STATUS KLINIS DAN DETEKSI LipL32 SAPI SEROPOSITIF LEPTOSPIROSIS DI KABUPATEN KULON PROGO Guntari Titik Mulyani; Wayan Tunas Artama; Estu Widodo
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55930

Abstract

  Leptospirosis is a zoonotic disease caused by Leptospira interrogans. Animals can act as carriers, spread leptospires in urine, and be a source of infection for other animals and humans. In leptospirosis cows can cause abortion, early birth, infertility, decreased milk production and death. The aims of this study was to determine the clinical status and detect the presence of leptospires with Polymerase Chain Reaction (PCR) from urine cows that are expressed as leptospirosis seropositive. A total of 12 cattle seropositive leptospirosis with Microscopic Agglutination Test (MAT) were carried out clinical examinations covering general conditions, pulsus examination, breathing and temperature and organ systems. The urine is collected aseptically, then DNA isolation is carried out using a kit from Genoid. Detection of leptospires in the urine is carried out by detecting the presence of the primary lipoprotein LipL32 making up the Leptospira membrane. The primer was designed with a 21-base forward forward: 5'-TGG ATC TGA TCA ACT ATT ACG-3 ‘containing 38.1% GC with Tm 57.2oC. 22 bases reverse reverse obtained: 5 '-CAC TTC ACC TGG TTT GTA GGT-3' containing GC 45.5% with Tm 62.1oC. Amplification was carried out as many as 40 cycles and continued with electrophoresis to determine the band formed at a wavelength of 506 bp. The results showed that all cows that were positive for leptospirosis with MAT were in a clinically healthy condition. In electrophoresis there are 7 out of 12 positive samples found in the urine leptospira indicated by the formation of a band at 506 bp. From the results of this study it can be concluded that leptospirosis-positive cows do not always show clinical symptoms, but have the potential to excrete leptospires along with urine, so they can act as a source of transmission of leptospirosis to humans, other animals, and the environment. 
Efficacy of Albendazole Against Strongylus sp. and Hematology Changes on Equine in Yogyakarta Special Region yuriadi yuriadi; Ida Tjahajati; Guntari Titik Mulyani; Kelviano Muqit; Aidah Rahmanita
Jurnal Sain Veteriner Vol 39, No 2 (2021): Agustus
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.60110

Abstract

The infestation of Strongylus sp. in horses can cause losses to horse breeders, including anorexia, anemia, gastrointestinal diseases and can cause death and decrease the horse population in DIY. Albendazole was a Benzimidazole preparation that is often used to treat worms in ruminants. This study also aims to determine the effect of Albendazole on blood images before and after treatment. The material used in this study were 10 horses with male and female sex,  over 3 years old, and infected with Strongylus sp. with an infestation rate of 200 EPG in faeces. Before treatment of drug was carried out, the faeces was examined with Mc Master method. After treatment with Albendazole, the worm eggs were examined three times at intervals of three days. Routine examination of worm eggs and blood was carried out at the Laboratory of the Department of Internal Medicine, Faculty of Veterinary Medicine, Universitas Gadjah Mada. The results showed that the EPG number decreased from the 0th, 3rd, 6th and 9th day of examinations. The average number of EPG on the 0th day was 990 eggs/gram, and the examination on the 9th day showed the number of eggs was 0 eggs/gram. The results of the hematology examination also showed no significant difference except for the high number of eosinophils on the 12th day after drug administration. The conclusion from this research is that Albendazole as a worm medicine can kill Srongylus sp. however, it does not affect the horse's hematology and health status.
Identifikasi Tipe Dehidrasi dan Profil Elektrolit Mayor pada Pasien Kucing di Rumah Sakit Hewan Prof. Soeparwi dan Beberapa Klinik Hewan di Wilayah Yogyakarta Guntari Titik Mulyani; Setyo Budhi; Kurnia .
Jurnal Sain Veteriner Vol 39, No 3 (2021): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.69901

Abstract

Dehydration is defined as a lack of body fluids followed by loss of electrolytes, and changes in acid-base balance. The type of dehydration is limited based on the concentration of sodium in serum at the time of dehydration. Maintenance of osmotic pressure and distribution of several compartments of body fluids are the main functions of the four major electrolytes, namely sodium (Na +), potassium (K +), chloride (Cl‾), and bicarbonate (HCO3‾). Sodium is the most cation in extracellular fluid, most potassium cation in intracellular fluid and chloride is the most anion in extracellular fluid. The purpose of this study was to identify the type of dehydration and to determine the major electrolyte profile in cats in Yogyakarta and its surroundings. This study used 18 sick cats that were thought to be dehydrated, marked by decreased skin turgor, CRT> 2 seconds, and 12 cats that were suspected of having electrolyte balance disorders with symptoms of ascites, uropoetic disorders. Blood was drawn for all cats to measure Pack Cells Volume (PCV) levels. Patient clinical data and patient diagnosis were recorded, cats with changes in serum PCV levels were separated for examination of levels of sodium, chloride, potassium using Seamaty SMT-120V. The type of dehydration is identified based on the sodium level in the serum of a dehydrated cat. The results showed that most of the cat patients were dehydrated had low serum sodium levels (hyponatremia). There was 1 cat patient had low chloride levels. Potassium levels in cats with UT obstruction increased, which led to a decrease in the Na: K ratio. Cat bicarbonate levels did not show any change. From the results of the study it was concluded that dehydration in cats at Prof. Soeparwi is hypotonic dehydration (71%). The sodium profile mostly decreased, chloride and bicarbonate levels did not change, while there were changes in potassium levels in patients with UT disorders. The advice given is to check electrolytes before doing fluid therapy. Prior to electrolyte testing, dehydrated cats can be given a sodium solution. 
Pembelian Ternak dan Kelembaban Tinggi Merupakan Faktor Risiko Leptospirosis pada Sapi di Girimulyo, Kulon Progo, Jogjakarta (PURCHASE OF CATTLE AND HIGH HUMIDITY WERE RISK FACTORS OF BOVINE LEPTOSPIROSIS IN GIRIMULYO, KULON PROGO DISTRICT) Guntari Titik Mulyani; Bambang Sumiarto; Yuriati .
Jurnal Veteriner Vol 15 No 2 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (96.602 KB)

Abstract

Leptospirosis is an acute infectious disease could to attack humans and animals such as dogs, cats,cows, pigs, and wild animals such as rats, weasels and squirrels. The death rate of human leptospirosis inIndonesia reached 2.5 to 16.5%. Leptospirosis attacked approximately 274 residents, 18 fatalities atKulonprogo disctrict, and designated as Extraordinary Events in 2011. The purpose of this reasearch is todetermine risk factors affecting the prevalence of leptospirosis in cattle in the area of human leptospirosisin Girimulyo, Kulon Progo. This study used 132 samples of cattle blood. Methods of examination withMicro Aglutination Test (MAT) conducted at the Center for Veterinary Research, Bogor. Micro AglutinationTest is done by creating a serum dilution with PBS in stages then added antigen in the form of various cultures leptospira serovar. Subsequently incubated at 28-30 ° C for 2 hours, and was reading the resultsunder a microscope dark field / phase contrast. Endpoint reading was 50% agglutination, highest finalserum dilution in serum-antigen mixtures that showed 50% agglutination is called the titer. Serum witha titer of 1:100 or more against one or more tested positive for serovar. Micro Aglutination Test result wasthe dependent variable (Y), while the independent variables (X) were factors of farmers, animal factors,enclosure factors, and feed factors. Univariate analysis was done to see descriptively each variable studied.Association between the prevalence of leptospirosis and risk factors was analyzed by Chi Square.Multivariate Logistic Regression Analysis was used to build models of leptospirosis disease to investigatethe risk factors associated with leptospirosis. Variables that influence the prevalence of leptospirosiswere measured magnitude of association by Odd Ratio. The results of study that the prevalence of bovineleptospirosis in Girimulyo subdistrict, Kulonprogo district was 7.6%. Risk factors for the prevalence ofleptospirosis in cattle in Girimulyo subdistrict, Kulonprogo district were livestock purchased factor (OR5.49) and a high humidity factor (OR 1.65). These results can be concluded that leptospirosis infection incattle is high (7.6%) in the area of human leptospirosis. The possibility cattle as a source of infection inhumans still needs further research.
Leptospirosis pada Kucing di Yogyakarta dan Sekitarnya (FELINE LEPTOSPIROSIS IN YOGYAKARTA AND ITS SURRONDING) Guntari Titik Mulyani; Slamet Raharjo; Agung Budi Purnomo; Yuli Santoso; Kurnia Kurnia; Dyah Kunti Wirapratiwi
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.737 KB) | DOI: 10.19087/jveteriner.2018.19.4.446

Abstract

Leptospirosis is a zoonotic disease that infects almost of mammals. The infecting microorganism is the Leptospira interrogans, which have many serovars. Clinical leptospirosis is rare in cats, but cats may shed leptospire in the urine to other animals, humans or environment. The aims of this research is to study feline leptospirosis in Yogyakarta and its surrounding. A total of 27 cats consisting of 3 groups of cats: household cats, stray cats, and cats with leptospirosis symptom were used in this research. The blood from all cats were taken 3 ml from cephalica vein, the serum were separated for Microscopic Agglutination Test (MAT). The examination were conducted in Bbalitvet Bogor for 14 Leptospira serovar: Ichterohaemorrhagiae, Javanica, Celledoni, Ballum, Pyogenes, Cynopeteri, Rachmati, Australis, Pomona, Canicola, Grippotyphosa, Bataviae, Hardjo, dan Tarrasovi. The results showed that 1 stray cat was positive for Tarrasovi serovar. From the results of this study can be concluded that there was subclinic feline leptospirosis in Yogyakarta that caused by Leptospira interrogans Tarrasovi serovar.
Kejadian Leptospirosis pada Anjing di Daerah Istimewa Yogyakarta (CASE OF CANINE LEPTOSPIROSIS IN THE CITY OF YOGYAKARTA) Guntari Titik Mulyani; Sri Hartati; Yuli Santoso; Kurnia Kurnia; Agung Budi Pramono; Dyah Kunti Wirapratiwi
Jurnal Veteriner Vol 18 No 3 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (85.099 KB) | DOI: 10.19087/jveteriner.2017.18.3.403

Abstract

Leptospirosis is a zoonotic disease, which is caused by Leptospira interrogans. The incidence of leptospirosis in dogs varies according to region and season, and is considered as emerging infectious diseases in humans. Clinical symptoms of leptospirosis in dogs vary greatly, some dogs are asymptomatic, with mild symptoms, and others progress to severe illness until death. The study aims to determine cases of canine leptospirosis in Yogyakarta, and identify the serovar that infect them. A total of 20 dogs without symptoms, with mild symptoms, to severe symptoms of leptospirosis were collected their serum and further tested using Microscopic Agglutination Test (MAT) conducted at the Center for Veterinary Research (BBLitvet) Bogor. History of vaccination is recorded as a consideration in interpreting the MAT results. The results showed that three out of the 20 samples were positive leptospirosis. Of the three positive samples, one was identified as serovar bataviae, another one as serovar bataviae and tarrasovi, and the last as serovar bataviae, tarrasovi, ichterohaemorrhagiae, canicola, celledoni, pyrogenes, cynopteri, and rachmati, respectively. The three dogs with leptospirosis showed similar clinical symptoms i.e. Anorexia, lethargy, and fever. It can be concluded that there are cases of canine leptospirosis in Yogyakarta which is predominantly caused by Leptospira interrogans serovar bataviae. ABSTRAK Leptospirosis adalah penyakit zoonosis, yang disebabkan oleh Leptospira interrogans. Kejadian leptospirosis pada anjing bervariasi menurut wilayah dan musim, dan dianggap sebagai penyakit menular yang muncul pada manusia. Gejala klinis leptospirosis pada anjing sangat bervariasi, beberapa anjing tanpa menunjukkan gejala, dengan gejala ringan, dan yang lain melanjut menjadi penyakit yang parah sampai kematian. Penelitian ini bertujuan untuk mengetahui kasus leptospirosis pada anjing di Propinsi Daerah Istimewa Yogyakarta, dan mengidentifikasi serovar yang menginfeksinya. Sebanyak 20 ekor anjing tanpa gejala, dengan gejala ringan, sampai gejala parah leptospirosis diambil serumnya guna pemeriksaan Microscopic Agglutination Test (MAT) yang dilaksanakan di Balai Besar Penelitian Veteriner (BBLitvet), Bogor. Sejarah vaksinasi dicatat sebagai bahan pertimbangan dalam menginterpretasi hasil pemeriksaan MAT. Hasil penelitian menunjukkan bahwa tiga dari 20 sampel positif leptospirosis. Satu sampel positif terhadap serovar Bataviae, satu sampel positif terhadap serovar Bataviae dan Tarrasovi, dan satu sampel lagi positif terhadap serovar Bataviae, Tarrasovi, Ichterohaemorrhagiae, Canicola, Celledoni, Pyrogenes, Cynopteri, dan Rachmati. Anjing yang positif leptospirosis menunjukkan gejala klinis yang sama berupa anoreksia, kelemahan, dan demam. Dari hasil penelitian ini dapat disimpulkan bahwa terdapat kasus leptospirosis pada anjing di Yogyakarta yang penyebabnya dominan oleh Leptospira interrogans serovar Bataviae.
Sosisalisasi Kesehatan Hewan Kepada Masyarakat Secara Daring: Online Socialization of Animal Health to The Community Soedarmanto Indarjulianto; Yanuartono Yanuartono; Slamet Raharjo; Alfarisa Nururrozi; Hastari Wuryastuty; Irkham Widiyono; Hary Purnamaningsih; Guntari Titik Mulyani; Ida Tjahajati; Sri Hartati; Yuriadi Yuriadi
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 2 No. 2 (2021): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v2i2.223

Abstract

ABSTRACT Various animal diseases are not always known by animal owners, so they must always be socialized even in the condition of the COVID-19 outbreak. This community service aims to provide animal health information to the community by online. This activity was carried out from in November 2020 using the Cisco Webex platform. The results showed that as many as 269 participants have joined in the socialization, consisting of 180 from Yogyakarta and 89 from outside Yogyakarta. Concluded that online socialization of animal health is successfully carried out and received a very good response from the community. Keywords: Animal health; Community service; Veterinary clinic ABSTRAK Berbagai macam penyakit hewan tidak selalu diketahui oleh pemilik hewan, sehingga harus selalu disosialisasikan walapun dalam kondisi wabah COVID-19. Pengabdian kepada masyarakat (PKM) ini bertujuan memberikan informasi kesehatan hewan kepada masyarkat secara daring. Pengabdian dilakukan pada bulan bulan Oktober sampai Nopember 2020 secara daring menggunakan platform Cisco Webex. Hasil PKM menunjukkan bahwa sebanyak 269 calon peserta telah mengikuti sosialisasi, yang terdiri dari 180 berasal dari Yogyakarta dan 89 dari luar Yogyakarta. Berdasar hasil kegiatan ini, disimpulkan bahwa sosialisasi tentang kesehatan hewan secara daring berhasil dilaksanakan dan mendapat respon sangat bagus dari masyarakat. Kata kunci: Kesehatan hewan; Klinik hewan; Pengabdian masyarakat.