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All Journal MEDIA PETERNAKAN - Journal of Animal Science and Technology ACTA VETERINARIA INDONESIANA Hemera Zoa Jurnal Sain Veteriner Jurnal Veteriner Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat ARSHI Veterinary Letters Indonesian Journal of Applied Research (IJAR) Jurnal Nukleus Peternakan Jurnal Veteriner dan Biomedis Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dedi Rahmat Setiadi
Departemen Klinik, Reproduksi, Dan Patologi; Fakultas Kedokteran Hewan, Institut Pertanian Bogor (IPB); Jalan Agatis Kampus IPB Dramaga, Bogor, Jawa Barat, INDONESIA, Kode Pos 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Veterinary

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Journal : Jurnal Veteriner

 1 
Validasi Kit Enzyme-Linked Immunosorbent Assay Komersial untuk Analisis Hormon Estradiol dan Progesteron Darah Kambing Kacang (VALIDATION OF COMMERCIAL ENZYME-LINKED IMMUNOSORBENT ASSAYKIT FORANALYSIS OF ESTRADIOLANDPROGESTERONE HORMONE IN BLOOD OF KACANG Dedi Rahmat Setiadi; Iman Supriatna; Muhammad Agil
Jurnal Veteriner Vol 15 No 4 (2014)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study was to evaluate the capability of two human commercial enzyme-linkedimmunosorbent assay (ELISA) kits(DRG International Inc.,Germany and GBC Taiwan) for measuringestradiol (E2) and progesterone (P4) in plasma of kacang goat. Three healthy and non pregnant femalekacang goats aged 2-3 years with regular estrous cycles were used in this study. Blood samples werecollected from the jugular vein using a 21G venoject every two days and it was intensified every day duringthe period before heat. Collected plasma were stored at -20ºC until the analysis. Capability validationwas conducted by measuring accuracy (parallelism test), sensitivity and precision.Parallelism test usingDRG commercial kit showed that sample curvewas parallel with standard curve of E2 and P4. In contrastit was not parallel with standard curve of GBC commercial kit. Sensitivity was measured from the lowesthormones concentration of E2 and P4 at 90% binding that were 25 pg/ml and 0.14ng/mL in DRG kit, whilein GBC kit were 5 pg/mL of E2 and 0.2 ng/mL of P4, respectively. Coefisien of variation of intra- andinterassay for both ELISA commercial kits were less than10%. It can be concluded that DRG commercialELISA kit E2 and P4 can be used to analyse female kacang goat blood plasma, while GBC commercialELISA kit E2 and P4 are not recommended.
Co-Authors Abdul Samik Afiff , Usamah Amani, Aqila Zata Amrozi Andriyono Kilat Adhi Anggraini Sukmawati Anriansyah Renggaman Atabani, Afton Bayu Febram Prasetyo Budianto Panjaitan Burhani, Al-May Abbyan Izzy Burhanuddin Burhanuddin Chairun Nisa Cristovao, Adelaide Jose Pereira Danang Dwi Cahyadi Dea Indriani Astuti Didid Diapari Dondin Sajuthi Dwi Rachmina Fariyanti, Anna Fatimah Fatimah Handri Dwi Agung Hastuti, Yohana Tri Herawati Herawati Heru Setijanto Iis Afriantini Imam Purwadi, Imam Iman Supriatna Jansen Manansang Jansen Manansang Laksono, Mohamad Nanang Tejo M Agus Setiadi Magfira Manansang, Willem Mokhamad Fakhrul Ulum, Mokhamad Muchidin Noordin Muchidin Noordin MUHAMMAD AGIL Mulia, Bongot Huaso Nurhidayat Prayetno, Saptohadi Putri Syifa Camilla R. Iis Arifiantini Raden I. Arifiantini Rhomadon, Gilang Riwu, Ronny Markus Jami Savitri Novelina Setyaningsih Rambu Liwa Setyaningsih Rambu Liwa Srihadi Agungpriyono Suharno Suharno Supratikno . Surya Agus Prihatno Tejolaksono, Muhammad Nanang Triana Gita Dewi Tuty L Yusuf Vincentia Trisna Yoelinda Widihasputri, Risti Yohana Tri Hastuti Yohana Tri Hastuti Yosua Kristian Adi