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All Journal Jurnal Akuakultur Indonesia Jurnal Pertanian Terpadu Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Jurnal Perikanan Universitas Gadjah Mada Indonesian Aquaculture Journal Jurnal Riset Akuakultur Aquacultura Indonesiana MARLIN : Marine and Fisheries Science Technology Journal SIGANUS: Journal of Fisheries and Marine Science Journal of Multidisciplinary Applied Natural Science Journal of Fisheries & Marine
ALIM ISNANSETYO
Universitas Gadjah Mada
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Energy Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Physics

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Journal : Journal of Fisheries

 1 
Susceptibility and Target Organ of Lymphocystis Disease Virus Infection in Giant Gourami (Osphronemus goramy), Hybrid Tilapia (Oreochromis sp.), Siamese Fighting Fish (Betta splendens), and Hybrid Catfish (Clarias sp.) Nikmah, Nur Lailatul Fitrotun; Isnansetyo, Alim; Istiqomah, Indah; Murwantoko, Murwantoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56685

Abstract

  Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish species 4. LCDV load is varied in quantity among different organs. Abstract Lymphocystis disease has a broad host range and has been reported to enter Indonesia. However, information regarding its susceptibility and predilection organs in fish is lacking. This study examined the susceptibility of four important fish species in Indonesia, namely, giant gourami (Osphronemus goramy), hybrid tilapia (Oreochromis sp.), Siamese fighting fish (Betta splendens), and hybrid catfish (Clarias sp.). The fish were infected with virus filtrate by intraperitoneal injection and immersion. The postinfection observation period was 60 days. Viral load was quantified by qPCR and expressed as major capsid protein (MCP) copy number/mg tissue. Mortality was observed in all fish species, with the highest recorded in hybrid catfish and the lowest in Siamese fighting fish. All the fish species showed changes in their clinical symptoms, such as anorexia and separation from schools. However, only giant gourami showed internal change seven days after injection (dpi), with white lesion detected in the liver. Viral load quantification showed that LCDV had different predilection organs in the four fish species. The highest viral load of giant gourami (1.7 x 104) was observed in the liver at 7 dpi, hybrid tilapia (7.5 x 103) was observed in the fins at 21 dpi, Siamese fighting fish (8.4 x 103) was observed in the fins at 14 dpi, and hybrid catfish (1.2 x 103) were observed in the fins and gills at 7 and 14 dpi. The findings indicated that giant gourami, hybrid tilapia, Siamese fighting fish, and hybrid catfish were susceptible to LCDV infection with different predilection organs.
Effect of Dietary Probiotic Lactococcus garvieae JAL37 Supplementation on Growth and Disease Resistance in Tor tambroides Challenged with Aeromonas hydrophila PTF3 Mahasin, Faiz; Istiqomah, Indah; Handayani, Desy Putri; Isnansetyo, Alim; Murwantoko, Murwantoko; Herath, Tharangani
Jurnal Ilmiah Perikanan dan Kelautan 2026: IN PRESS ISSUE (JUST ACCEPTED MANUSCRIPT, 2026)
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v18i2.82534

Abstract

Graphical Abstract Highlight Research 1. Aeromonas hydrophila PTF3 was identified and analysed as a causative agent of a previously identified hemorrhagic syndrome in Tor tambroides. 2. Lactococcus garvieae JAL37 supplementation in feed can enhance the growth of Tor tambroides fingerlings. 3. Lactococcus garvieae JAL37 can increase the resistance of Tor tambroides fingerlings to Aeromonas hydrophilla PTF3. 4. Relative mRNA levels of ghrelin, GH, and I-FABP genes were increased after 90 days of tor cultivation with Lactococcus garvieae JAL37 supplementation.   Abstract  Mahseer, or tor fish, an indigenous Asian fish of great socio-economic value, is gaining attention as it faces threats to its survival. However, slow growth rates and disease susceptibility remain significant challenges in mahseer cultivation. Feed supplemented with probiotic Lactococcus garvieae JAL37 at a concentration of 1.0 × 10⁸ CFU/g feed, twice weekly, was used in this study to evaluate its effects compared with a control. Triplicate groups of Tor tambroides fingerlings (average weight 1.86 ± 0.09 g) were cultivated for 90 days. Parameters such as growth, intestinal histology, and mRNA expression levels of growth-related genes (ghrelin and I-FABP from the intestine; GH and IGF-1 from the liver) were assessed. Day 90 post-cultivation, mahseer were challenged with an oral infection of Aeromonas hydrophila at 5.0 × 105 CFU/g fish to assess survival rate and mRNA expression levels of pro-inflammatory cytokine-related genes (TNF-α and IL-1β). Weight gain, specific growth rate, and villus length in Tor tambroides fingerlings treated with probiotics showed significant increases (P<0.05). Real-time PCR results indicated upregulated mRNA expression of ghrelin, I-FABP, and GH genes in the probiotic group, whereas IGF-1 gene expression was similar to that in the control group. Probiotic treatment significantly improves the survival rate of T. tambroides after A. hydrophila challenge, while suppressing mRNA expression of TNF-α and IL-1β genes post-challenge. These findings suggest that Lactococcus garvieae JAL37 supplementation can promote growth in Tor tambroides fingerlings and enhance disease resistance against Aeromonas hydrophila.
Co-Authors Abdullah, Faisal Zain Amir Husni Angela Mariana Lusiastuti Anshory, Lutfi Bambang Triyatmo Buana Basir Condro, Sri Laksono Eny Heriyati, Eny Esti Handayani Hardi Gina Saptiani Halim, Aldi M. Handayani, Desy Putri Hariyadi, Dimas Rizky Herath, Tharangani Ign. Hardaningsih INDAH ISTIQOMAH Irpani, Husni Mubarok Ismail, Norshida Jabbar, Firmansyah Bin Abd Kariyanti, Kariyanti Kim, Sung Sam Kristiani, Theresia Adven Dea Latifah, Ma'rufa Nurul Lusiana, Lusiana Mahasin, Faiz Mira Mawardi Muhammad Nursid Mukhlisnah Djalil Murti, Fauziah Arelia Usmiara Murwantoko . Niklani, Andi Noer Kasanah Nugroho, Hartanto Nur Lailatul Fitrotun Nikmah Nuri Muahiddah Nurrahmi Dewi Fajarningsih, Nurrahmi Dewi Putra, Hemi Adi Rahayu, Widianingsih Rarastoeti Pratiwi Rina Puji Astuti Rudy Agung Nugroho Rustadi Rustadi Senny Helmiati SUWARNO HADISUSANTO Syakuri, Hamdan Tito Arya Nugraha Tri Rini Nuringtyas Triastutik, Gemi Triyanto Triyanto Wahyudi Wahyudi Wulansari, Tyas Ayu Yasa, Ngurah Sedana Yasa, Ngurah Sedana Zafran, Zafran