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All Journal Jurnal Fitopatologi Indonesia Vegetalika Jurnal Ilmu Pertanian BIOTROPIA - The Southeast Asian Journal of Tropical Biology AL KAUNIYAH Indonesian Journal of Biotechnology Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA JPT : JURNAL PROTEKSI TANAMAN (JOURNAL OF PLANT PROTECTION) jurnal Ilmu Pertanian (Agricultural Science)
Siti Subandiyah
Cultivation Of Agriculture Department, Faculity Of Agriculture, Gadjah Mada University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Veterinary

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The Use Of Pulse Field GelElectrophoresis For Megaplasmid Isolatioan Of Pseudomonas solanacearum Siti Subandiyah; Triwibowo Yuwono; Wayan T. Artama
Jurnal Ilmu Pertanian Vol 5, No 4 (1994): September
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.60011

Abstract

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Exploration, Screening, and Application of Silica Solubilizing Bacteria and Silica Fertilizer to Suppress Fusarium Wilt Disease in Abaca Werdiningtyas, Cyrilla Kinanti; Wibowo, Arif; Subandiyah, Siti; Widiastuti, Ani
Jurnal Fitopatologi Indonesia Vol 19 No 3 (2023): Mei 2023
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.19.3.111-117

Abstract

Exploration, Screening, and Application of Silica Solubilizing Bacteria and Silica Fertilizer to Suppress Fusarium Wilt Disease in Abaca Abaca (Musa textilis) is producing high quality natural fibre with the main problem is fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense TR4. The study aims to obtain silica solubilizing bacteria (SSB), determine the role of SSB and silica fertilizer to inhibit fusarium and to stimulate plant growth. Six isolates of SSB were obtained from healthy abaca rhizosphere, three of them had antagonistic activity against F. oxysporum f. sp. cubense TR4. The four treatments tested showed no different severity of fusarium wilt. Likewise, the four treatments did not show any difference in the observed growth variables. Silica solubilizing bacteria and silica fertilizer have not been able to control F. oxysporum f. sp. cubense TR4. Therefore, abaca proved to be susceptible to F. oxysporum f. sp. cubense TR4.
Comparison of Two Huanglongbing Detection Methods in Samples with Different Symptom Severity: Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Sariasih, Yenny; Subandiyah, Siti; Widyaningsih, Sri; Khurshid, Tahir; Mo, Jianhua; Donovan, Nerida
Jurnal Fitopatologi Indonesia Vol. 20 No. 4 (2024): Juli 2024
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.20.4.174-186

Abstract

Perbandingan Dua Metode Deteksi Huanglongbing pada Sampel dengan Tingkat Keparahan Gejala yang Berbeda Huanglongbing atau citrus greening di Asia disebabkan oleh patogen Candidatus Liberibacter asiaticus (CLas) merupakan salah satu penyakit jeruk yang paling menghancurkan di seluruh dunia. Penyakit ini menjadi salah satu penyebab penurunan produksi jeruk di Indonesia. Gejala huanglongbing pada tanaman jeruk di lapangan dan rumah kaca ternyata memiliki tingkat keparahan yang berbeda. Penelitian ini dilakukan untuk mendeteksi CLas pada beberapa jenis jeruk berdasarkan gejala pada daun menggunakan PCR konvensional dan real-time (qPCR). Tiga pasang primer digunakan dalam penelitian ini, sepasang Las606/LSS untuk PCR konvensional dan dua pasang untuk qPCR, yaitu Las931/LSS dan Lj900F/Lj900R. Hasil penelitian menunjukkan bahwa blotchy mottle adalah gejala huanglongbing yang paling mudah dideteksi dan ditemukan di kebun dan rumah kaca. Pasangan primer Lj900F/Lj900R lebih cocok untuk mendeteksi patogen CLas menggunakan qPCR daripada Las606/LSS berdasarkan kurva leleh dan Ct value yang muncul. Deteksi qPCR lebih akurat dan sensitif bahkan dengan konsentrasi DNA yang lebih sedikit. Batas bawah Ct value sampel daun sehat ialah 34.08. Daun jeruk dinyatakan positif jika Ct value kurang dari 34.08. Ct value berdasarkan tingkat keparahan atau dengan skoring antara HLB daun simtomatik dari lapangan dan rumah kaca menunjukkan perbedaan yang cukup besar, yaitu Ct value sampel simtomatik dari lapangan lebih rendah daripada sampel rumah kaca.
Incidence of twisted disease and cultivation practice of shallot farmers in Bantul coastal area, Yogyakarta, Indonesia Wibowo, Arif; Santika, Islaminati Anna; Syafitri, Luthfiana Mifta; Widiastuti, Ani; Subandiyah, Siti; Harper, Stephen
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 23 No. 1 (2023): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12323-30

Abstract

Shallot (Allium cepa var. aggregatum L.) is an important commodity in horticulture and Indonesian foods. In Bantul Regency, Yogyakarta, Indonesia, shallot twisted diseases caused by Fusarium spp. significantly reduced yield quality and quantity. Supportive condition for plant-pathogen interaction needed for twisted disease development. This research aimed to measure the twisted disease incidence and observe the cultivation practice applied by shallot farmers in the Bantul coastal area as environmental aspects of the twisted disease to be a pre-study for further research on metagenomic analysis. This research had conducted in three sub-villages with different agroecosystems in the coastal area: Sono, Samiran, and Depok. Data were collected in shallot cultivation period in dry and rainy seasons from three farmers of each area. The higher disease incidence in the rainy season occurred in the Samiran sub-village at 33.97%, while in the dry season, disease incidence occurred in the Depok sub-village at 20.14%. Sono sub-village had the lowest disease incidence in rainy and dry seasons at 12.44% and 0%. Farmer cultivation practice may drive disease incidence due to environmental factors such as shallot variety, spacing between plants, fertilizer, and fungicides. Hence, further study is needed to understand plant-pathogen interaction with metagenomics through samples from 2 and 6 WAP in rainy seasons.
Response of five shallot varieties applied with Bacillus spp. against twisted disease Pratiwi, Annisa Hasta; Wibowo, Arif; Joko, Tri; Widiastuti, Ani; Subandiyah, Siti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12417-27

Abstract

The use of several shallot varieties applied with the biological agent Bacillus spp. is one of the most developed methods of controlling twisted disease, as it is safe and efficient. The large number of shallot varieties released to farmers requires the selection of varieties with the best resistance response to twisted disease. This study aimed to determine the different responses between five local shallot varieties treated with a combination of Bacillus velezensis B-27 and B. cereus RC76 against the twisted disease. This study was conducted in a greenhouse and on the field using Tajuk, Bima Brebes, Bauji, Crok Kuning, and Manjung varieties, which were dipped and sprayed with a B. velezensis B-27 and B. cereus RC76. The treatment of B. velezensis B-27 and B. cereus RC76 on five varieties showed a good response to suppressing twisted disease. The twisted disease incubation period in five varieties treated with the combination of B. velezensis B-27 and B. cereus RC76 showed a slower result than the control, the disease incidence and intensity could be reduced by 70 90%. The best resistance response of varieties treated with the combination of B. velezensis B-27 and B. cereus RC76 was shown by Tajuk compared to the other four varieties.
Primer Design of Volatile Synthesis Coding Genes in Ralstonia syzygii subsp. celebesensis Damanik, Nina Septania; Prakoso, Ady Bayu; Triyana, Kuwat; Subandiyah, Siti
Jurnal Perlindungan Tanaman Indonesia Vol 27, No 2 (2023)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.81099

Abstract

Microbes produce various types of volatile organic compounds (VOCs) through metabolism, which can be used for diagnostic purposes. Microbes' types and classes of VOCs are very wide, including fatty acid derivatives (hydrocarbons, alcohols, and ketones), aromatic compounds, nitrogen-containing compounds, and volatile sulfur compounds. Microbial volatile organic compounds (VOCs) can also be divided into several chemical classes: alkenes, alcohols, ketones, benzos, pyrazines, sulfides, acids, esters, and terpenes. This study aimed to design primers for genes encoding volatile synthesis in Ralstonia syzygii subsp. celebesensis, which causes blood disease in the banana plant. Some of the genes involved are adc (acetone synthesis), adhP (ethanol synthesis), ilvA, nirBD (ammonia synthesis), mdcA (propionic acid synthesis), cysI (hydrogen sulfide synthesis), and speBC (putrescine synthesis). Primers were designed and examined for specificity in silico using Primer3Plus, Geneious Prime, and BLAST programs. The numbers of nine pairs designed primers were successfully amplifying the related nine VOC genes of R. syzygii subsp. celebesensis for qPCR. 
Pathogenicity and Detection of Phytohormone (Gibberellic Acid and Indole Acetic Acid) Produced by Fusarium spp. that Causes Twisted Disease in Shallot Lestiyani, Ayu; Wibowo, Arif; Subandiyah, Siti
Jurnal Proteksi Tanaman (Journal of Plant Protection) Vol. 5 No. 1 (2021): June 2021
Publisher : Plant Protection Department, Faculty of Agriculture, Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jpt.5.1.24-33.2021

Abstract

The twisted disease is one of the essential diseases in shallots caused by Fusarium spp. This study aimed to study pathogenicity and identify Fusarium species isolated from shallot plants with twisted symptoms in Nganjuk and Bantul areas. The Fusarium isolates were identified and then tested for pathogenicity levels and the effect of the hormones GA3 and IAA on shallot symptoms. Molecular identification using NF2 and NF4 successfully identified one isolate of Fusarium oxysporum, three isolates of F. acutatum, and three isolates of F. solani. Each of these species produces different symptoms. Pathogenicity test showed that all isolates had disease incidence reaching 100%, except isolates of F. solani1 causing wilt and F. solani3 causing twisted have the lower disease incidence were 77.8% and 77.7%, respectively. The investigation caused twisted shallot related to different symptoms was tested using the Thin Layer Chromatography (TLC) method. The result indicates that all isolates did not find IAA hormone. In contrast, the hormone GA3 was found in F. solani2 and F. solani3 isolates, caused bulb rot and twisted disease, respectively. Detection of IAA, GA3, and other hormones in shallot plants showed different symptoms should be studied further.
Enrichment of organic material with Trichoderma asperellum for the management of twisted disease on shallot Ilma, Hurin Nabila Aghnia; Wibowo, Arif; Joko, Tri; Subandiyah, Siti; Harper, Stephen
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 24 No. 1 (2024): MARCH, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA: JOURNAL OF TROPICAL PLANT PE
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jhptt.12428-37

Abstract

Shallots are a type of plant used as a flavoring agent and are often affected by twisted disease caused by Fusarium spp. This study aims to determine the ability of organic material enriched by Trichoderma asperellum in controlling twisted disease, increasing production, and suppressing the population of Fusarium spp. in the soil. The research showed that compost and chicken manure enriched with T. asperellum in a ratio (200:1) were able to reduce the disease incidence compared to controls, in addition to increasing production and suppressing the population of Fusarium spp. in the soil. The colonies of Fusarium spp. in the soil were correlated with disease incidence in each treatment. The highest incidence of twisted disease was followed by the number of Fusarium spp. colonies. The microbial composition did not affect disease suppression when observed using a dependent method using PCR-RISA. The microbial composition with the dependent method using PCR RISA did not affect the suppression of the twisted disease in the shallot plants.
Motility and Biofilm of Ralstonia syzygii subsp. celebesensis, the Causative Agent of Banana Blood Disease Saraswati, Vivian Thea; Subandiyah, Siti; McCann, Honour C.; Joko, Tri
Jurnal Perlindungan Tanaman Indonesia Vol 29, No 1 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.103868

Abstract

Blood disease in bananas is caused by Ralstonia syzygii subsp. celebesensis. This disease is a significant phytopathological concern, leading to deterioration of the plant's xylem tissue. Symptoms observed in infected plants typically begin from the shoot and banana inflorescence and progressively spread to other parts of the plant. The spread of these symptoms may result from the motility of R. syzygii subsp. celebesensis within the vascular system. This study aims to investigate the bacterial motility and biofilm formation ability, both contributing to the blockage of water and nutrient flow in the plant. The research methods included bacterial cultivation, molecular detection, motility assays (swimming and swarming tests), as well as biofilm and pellicle formation assay. The results indicated that R. syzygii subsp. celebesensis exhibited sliding motility but did not demonstrate swarming ability. Additionally, the bacterium was capable of forming both biofilm structures and pellicle layers. These findings provide insights into the role of motility types and colony structure formation in the virulence of R. syzygii subsp. celebesensis, potentially influencing the infection process in banana plants. Furthermore, this study's results are expected to contribute to developing improved disease management strategies.
Hyper-Solubilizing Tricalcium Phosphate Mutants of Klebsiella sp. GMD08 Ariyanti, Nur Fitriana; Widada, Jaka; Subandiyah, Siti
BIOTROPIA Vol. 27 No. 3 (2020): BIOTROPIA Vol. 27 No. 3 December 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (293.952 KB) | DOI: 10.11598/btb.0.0.0.1150

Abstract

Klebsiella sp. GMD08 is a bacterium capable of dissolving insoluble inorganic phosphate into soluble phosphate ions through its organic acid production. Transposon is a genetic element commonly used to generate mutants through mutagenesis, and thus can be used to identify genetic functions involved in phosphate-solubilizing mechanisms. This research was conducted to identify the genes of Klebsiella sp. GMD08 involved in phosphate solubilization through sequence detection obtained from a hyper-solubilizing phosphate mutant library. Mutation was carried out by inserting a mini-Tn5 transposon hosted in Escherichia coli S17-1/λpir [pBSL202] into the chromosome of Klebsiella sp. GMD08 using the filter mating conjugation method. Transconjugant mutant candidates were then qualitatively and quantitatively analyzed for their ability to dissolve tricalcium phosphate [Ca₃(PO₄)₂] using Pikovskaya medium. The organic acid profiles of the transconjugant mutants were detected using High-Performance Liquid Chromatography (HPLC). Suspected genes involved in phosphate solubilization were identified through sequencing of transposon insertion sites. Nucleotide BLAST was used to determine nucleotide sequence similarity with the database. The results showed that PB116 and PB122 were the two main transconjugant mutants obtained from transposon mutagenesis with higher tricalcium phosphate-dissolving ability. Gluconic acid was identified as the main organic acid produced by Klebsiella sp. GMD08 in its phosphate-solubilizing mechanism. Furthermore, the arginine repressor (ArgR) and malate dehydrogenase (mdh) genes were found to be involved in the phosphate-solubilizing mechanism of Klebsiella sp. GMD08.
Co-Authors A. C. Hayward Abd. Rasyid Syamsuri Ani Widiastuti, Ani Arif Wibowo Arif Wibowo Ariyanti, Nur Fitriana Christanti Sumardiyono Damanik, Nina Septania Donny Widianto Donovan, Nerida Eka Tarwaca Susila Putra Endang Sulistyaningsih Harper, Stephen Ilma, Hurin Nabila Aghnia JAKA WIDADA Khurshid, Tahir Kuwat Triyana Lestiyani, Ayu Liliek Sulistyowati M. Fegan Margo Sulistio McCann, Honour C. Mo, Jianhua Nur Edy Nurviani Nurviani Peter Taylor Prakoso, Ady Bayu Pratiwi, Annisa Hasta Ramot Christian Santika, Islaminati Anna Saraswati, Vivian Thea Sri Sulandari Sri Widyaningsih Susamto Somowiyarjo Syafitri, Luthfiana Mifta Tri Joko Tri Joko Triastuti Rahayu Triwibowo Yuwono Utik Windari Wayan T. Artama Werdiningtyas, Cyrilla Kinanti Yekti Asih Purwestri Yenny Sariasih