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All Journal HAYATI Journal of Biosciences Jurnal Pendidikan dan Pembelajaran Khatulistiwa (JPPK) Jurnal Perikanan Universitas Gadjah Mada Aquacultura Indonesiana Journal of Aquaculture and Fish Health SIGANUS: Journal of Fisheries and Marine Science Journal of Fisheries & Marine
INDAH ISTIQOMAH
Departemen Perikanan, Fakultas Pertanian, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Education Immunology & microbiology Languange, Linguistic, Communication & Media Mathematics Other

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Safety, Adherence, Enzymatic Activities, and Application Effects of Oral Probiotic Candidates for Shortfin Eel (Anguilla bicolor bicolor) Andita Ratih Dewanti; Anggi Octari Putri; Indah Istiqomah Istiqomah; Alim Isnansetyo
Jurnal Ilmiah Perikanan dan Kelautan Vol. 14 No. 2 (2022): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v14i2.34315

Abstract

Highlight Research The shortfin eel elver bicolor bicolor was tested for the safety of Enterobacter sp. JC05, Lactococcus sp. JAL37, and Bacillus sp. PCP1 The ability of bacterial strains to adhere to epithelial cells of shortfin eel epithelial cells was demonstrated The bacterial strains' proteolytic, cellulolytic, and lipolytic activities were detected Oral administration of the bacterial cocktail lowered overall viable bacterial count but did not affect the shortfin eel's intestinal histological characteristics   Abstract Aquaculture of the shortfin eel (Anguilla bicolor bicolor) has been plagued by low survival and growth due to the low tolerance to water quality and feed. The microbiota and shape of the fish intestinal tract influence the immune and digestive systems. The use of bacterial probiotics is fascinating to enhance the digestion system. This study aimed to characterize bacterial probiotic candidates' safety and potential probiotic features for shortfin eel (A. bicolor bicolor) aquaculture. The safety, adherence, and enzymatic activity of three bacterial strains (Bacillus sp. PCP1, Lactococcus sp. JAL 37, and Enterobacter sp. JC05) were investigated. An oral application test was performed on shortfin eel (n=880, 15 g) every four days with 0, 3x103, 3x105, and 3x107 CFU/g diet dosages in quadruplicates for two months. At the end of the experiment, total cultivable bacteria and intestinal morphology were assessed. Based on the hemolytic test and intraperitoneal injection, the bacterial strains were considered harmless. In an in vitro investigation, the bacteria attached to shortfin eel intestinal epithelial cells. An agar diffusion method validated the proteolytic, lipolytic, and cellulolytic activities. In vivo feeding tests with the bacterial cocktail lowered the total viable bacteria in the gut while preserving the gut histology. Bacterial strains of the present study are potential probiotic candidates for shortfin (A. bicolor bicolor) aquaculture.
Sodium Alginate from Padina australis Modulates Innate Immune and Immune Gene Expression in Red Tilapia (Oreochromis sp.) Toni Kuswoyo; Alim Isnansetyo; Muwantoko Murwantoko; Amir Husni; Indah Istiqomah
Jurnal Ilmiah Perikanan dan Kelautan Vol. 15 No. 1 (2023): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v15i1.40943

Abstract

Highlight Research Development of immunostimulant from natural sources is a promising approach to improve the immune status of fish Dietary administration of sodium alginate from Padina australis at 2.0 g/kg was an effective immunostimulant for red tilapia Red tilapia fed diet with sodium alginate significantly increased innate immune responses of TLC, PA, respiratory burst, SOD, TPP, lysozyme, and ACH50 activity Red tilapia fed diet with sodium alginate significantly modulated immune genes expression of GPx, TNF-α, IFN-γ, IL-10, TGF-β1, and Hepcidin Abstract The study of immunostimulant derived from natural sources has received considerable attention in recent years. Indonesian coasts have various sources of immunostimulant, especially brown algae, which are rich in alginate. This research investigated innate immune response and immune genes in red tilapia (Oreochromis sp.) after being fed diets supplemented with sodium alginates (SA) from a brown alga, Padina australis, originating from Awur Bay, Jepara, Indonesia. This work provided comprehensive information regarding the interaction of cellular-humoral immunity and immune gene expression. Red tilapia was fed with SA-containing diet at doses of 0, 2, 3 and 4 g/kg for 12 days, then blood samples were collected on days 0, 3, 6, 9, and 12 to evaluate the total leucocyte count (TLC), phagocytic activity/index (PA/PI), respiratory burst, superoxide dismutase (SOD), lysozyme, alternative complement (ACH50) activity, total plasma protein (TPP), and immune genes expression (blood sample on day 6). The immunological parameters improved in response to the administered doses and period. Dietary SA enhanced total leucocyte count, phagocytic activity, respiratory burst, superoxide dismutase, lysozyme, and ACH50 activity, whereas the phagocytic index and total plasma protein were not significantly different. Furthermore, the mRNA levels of antioxidant-related gene (GPx), pro-inflammatory cytokines (TNF-α and IFN-γ, except for IL-1β), and antimicrobial peptides (Hepcidin) were upregulated. Meanwhile, anti-inflammatory cytokines (IL-10 and TGF-β1) were downregulated. Supplementation SA diet at 2.0 g/kg as the lowest dose revealed the most effective effects on innate immune and immune genes expression. Dietary SA was a potential immunostimulant in red tilapia aquaculture.
Toksisitas Daun Miana (Coleus scutellarioides (L) Benth) Sebagai Antibakteri Pada Udang Vannamei (Litopenaeus vannamei) Basir, Buana; Isnansetyo, Alim; Istiqomah, Indah; Jabbar, Firmansyah Bin Abd
SIGANUS: Journal of Fisheries and Marine Science Vol 1 No 2 (2020): SIGANUS: Journal of Fisheries and Marine Science
Publisher : Universitas Sulawesi Barat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31605/siganus.v1i2.653

Abstract

Tujuan Penelitian ini untuk menganalisis toksisitas daun miana sebagai antibakteri pada udang vanamei. Penelitian ini dilaksanakan pada Maret sampai Oktober 2019, di Laboratorium Sekolah Tinggi Teknologi Kelautan (STITEK) Makassar. Sampel daun miana berasal dari beberapa daerah di Sulawesi Selatan. Uji aktivitas antibakteri ekstrak daun miana dengan tiga fraksi, yaitu ethanol, n-hexane, dan chloroform menggunakan metode difusi kertas cakram dengan konsentrasi ekstrak 500 µg/mL dan 1000 µg/mL, sebanyak tiga kali ulangan. Uji toksisitas metode BST (LC70) dengan konsentrasi uji 2500, 1250, 625, 312.5, 156.25, 78.125 µg/mL. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa uji aktivitas ekstrak daun miana tertinggi pada fraksi chloroform, dengan diameter hambat 28 mm. Sementara pada antibiotik oxytetracycline diameter hambat sebesar 31 mm. Nilai toksisitas pada tingkat mortalitas 30% pada konsentrasi 1250 µg/mL. Ekstrak daun miana fraksi chloroform mampu menggantikan peran antibiotic oxytetracycline dengan penggunaan konsentrasi dibawah 1250 µg/mL.
Agarolytic Bacillus sp. FRAgK1 Screened from Gracilaria (Rhodophyta) Thallus as Probiotic Candidate for Abalone Abdullah, Faisal Zain; Istiqomah, Indah; Isnansetyo, Alim; Yasa, Ngurah Sedana; Ismail, Norshida
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.93662

Abstract

Agarolytic bacteria produce agarase, which may aid in the growth of cultured tropical abalone fed natural seaweed. Agarolytic bacteria can come from a variety of sources, such as seawater, abalone intestines, and dead seaweed. This study aimed to isolate, screen, describe, and identify agarolytic bacteria found in red macroalgae. Agarolytic bacteria isolated from Gracilaria segregated from the substrate at Drini Beach, Gunungkidul Regency, were qualitatively described using the agarolytic index, antibiotic susceptibility, acid resistance (pH 4), and safety test. We collected seven agarolytic isolates. FRAgK1 isolate had the highest agarolytic index, was sensitive to antibiotics, resistant to low pH conditions, and non-pathogenic to finfish, making it suitable for use as gut probiotics in abalone. The bacterium was short rod-shaped, Gram-positive, non-motile, lacked catalase and indol, and was unable to ferment lactose or sucrose. The 16S rRNA gene sequence of FRAgK1 was most like Bacillus subtilis, however only by 99.43%.
Susceptibility and Target Organ of Lymphocystis Disease Virus Infection in Giant Gourami (Osphronemus goramy), Hybrid Tilapia (Oreochromis sp.), Siamese Fighting Fish (Betta splendens), and Hybrid Catfish (Clarias sp.) Nikmah, Nur Lailatul Fitrotun; Isnansetyo, Alim; Istiqomah, Indah; Murwantoko, Murwantoko
Jurnal Ilmiah Perikanan dan Kelautan Vol. 16 No. 2 (2024): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v16i2.56685

Abstract

  Highlight Research 1. Lymphocystis disease is reported to infect seawater and freshwater fishes 2. The four important freshwater fish species in Indonesia are evaluated on their susceptibility to Lymphocystis Disease Virus 3. Lymphocystis infection causes behavioural changes and mortality with different onset times after infection on the four fish species 4. LCDV load is varied in quantity among different organs. Abstract Lymphocystis disease has a broad host range and has been reported to enter Indonesia. However, information regarding its susceptibility and predilection organs in fish is lacking. This study examined the susceptibility of four important fish species in Indonesia, namely, giant gourami (Osphronemus goramy), hybrid tilapia (Oreochromis sp.), Siamese fighting fish (Betta splendens), and hybrid catfish (Clarias sp.). The fish were infected with virus filtrate by intraperitoneal injection and immersion. The postinfection observation period was 60 days. Viral load was quantified by qPCR and expressed as major capsid protein (MCP) copy number/mg tissue. Mortality was observed in all fish species, with the highest recorded in hybrid catfish and the lowest in Siamese fighting fish. All the fish species showed changes in their clinical symptoms, such as anorexia and separation from schools. However, only giant gourami showed internal change seven days after injection (dpi), with white lesion detected in the liver. Viral load quantification showed that LCDV had different predilection organs in the four fish species. The highest viral load of giant gourami (1.7 x 104) was observed in the liver at 7 dpi, hybrid tilapia (7.5 x 103) was observed in the fins at 21 dpi, Siamese fighting fish (8.4 x 103) was observed in the fins at 14 dpi, and hybrid catfish (1.2 x 103) were observed in the fins and gills at 7 and 14 dpi. The findings indicated that giant gourami, hybrid tilapia, Siamese fighting fish, and hybrid catfish were susceptible to LCDV infection with different predilection organs.
Natural Spawning Breeding Techniques Analysis of Carp (Cyprinus carpio) in the Fish Seed Center (BBI) Bolangan, Tabanan, Bali Kusmiatun, Anik; Arsana, I Made Putra; Istiqomah, Indah
Journal of Aquaculture and Fish Health Vol. 13 No. 2 (2024): JAFH Vol. 13 No. 2 June 2024
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jafh.v13i2.47505

Abstract

Carp (Cyprinus carpio) is a freshwater fish species with a high tolerance to various environmental conditions. The present study aimed to analyze the seeding process of carp using a natural spawning method at the Fish Seed Center (BBI) Bolangan, Tabanan, Bali. The descriptive analysis had been used to analyze of the data collected through active participation, observation, and interviews. The carp natural spawning techniques at BBI Bolangan were conducted in an earthen pond. The breeding techniques include pond preparation, brood stock selections, fish spawning, larvae or seed maintenance, water quality control, disease control, and fish harvesting. Male and female parents in a 2:1 ratio were required for effective breeding. This natural hatching system produced up to 100806 eggs with a hatching rate of 74.4%, yielding 75000 larvae per female parent. After a week of care, the larvae have a 30% survival rate and are ready for cultivation in the following section (nursery II). We conclude that the Carp (Cyprinus carpio) breeding techniques at the Fish Seed Center (BBI) Bolangan, Tabanan, Bali are technically sound and feasible to apply.
Modulation of Gut Microbiota, Nutrient Transport Gene Expression, and Growth of Red Hybrid Tilapia (Oreochromis sp.) Fed with Black Soldier Fly (Hermetia illucens) by Oral Probiotics Latifah, Ma'rufa Nurul; Murti, Fauziah Arelia Usmiara; Helmiati, Senny; Ismail, Norshida; Syakuri, Hamdan; Istiqomah, Indah; Murwantoko, Murwantoko; Isnansetyo, Alim
Jurnal Perikanan Universitas Gadjah Mada Vol 26, No 2 (2024)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfs.95268

Abstract

Maggot Black Soldier Fly (BSF) is a nutritionally rich alternative food for fish. The slow digestibility requires the combination with other technologies to produce fish with excellent growth. This study aims to investigate the impact of probiotic application on the growth, microbiome, and gene expression in the intestines of tilapia-fed black soldier fly (BSF) larvae. Tilapia intestines were examined after two months of rearing with three replications and two treatments: A) 30% maggots and 70% commercial pellets with probiotics, and B) 30% maggots and 70% commercial pellets without probiotics. We discovered that probiotics have a significant impact on the gut microbiomes of fish and their absolute growth. The amplicon sequence variant in the probiotic treatment (A) was 25, with dominance by Cetobacterium, Acinetobacter, Enhydrobacter, and Gemmobacter, while the non-probiotic treatment (B) was 8, with dominance by Cetobacterium and Turicibacter. The probiotic treatment increased the expression of Ghrelin, Muc-2, IL-1β, and I-FABP genes, but not the CD36 gene. These findings suggest oral probiotics can help boost tilapia production when fed maggot black soldier fly (Hermetia illucens).
Co-Authors . MUHTADI . TRIYANTO Abdullah, Faisal Zain Ahmad Fauzi Rohman Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo Alim Isnansetyo ALIM ISNANSETYO Amir Husni Andita Ratih Dewanti Anggi Octari Putri ANIK KUSMIATUN Arsana, I Made Putra Bambang BU Bambang Genjik S Bambang Triyatmo Buana Basir Deendarlianto Hariyadi, Dimas Rizky Ign. Hardaningsih Imelda Novita Atitus Ismail, Norshida Jabbar, Firmansyah Bin Abd Kamiso H. Nitimulyo Kamiso Handoyo Nitimulyo Kamiso Handoyo Nitimulyo KAMISO HANDOYO NITIMULYO Karunia Adetera Nungki Wijayanti Kim, Sung Sam Latifah, Ma'rufa Nurul Muhammad Murdjani Muhammmad Murdjani Murti, Fauziah Arelia Usmiara Murwantoko . Murwantoko Murwantoko Muwantoko Murwantoko Nikmah, Nur Lailatul Fitrotun Retno Widaningroem Riesa Yosita Rina Anggraeni Safia Rozi Rozi Rustadi Rustadi Senny Helmiati Senny Helmiati Shima Bhaskara Ayuningrum Syakuri, Hamdan Toni Kuswoyo Triyanto Triyanto Triyanto Triyanto Triyanto Triyanto Wahyudi Wahyudi Wiratni Budhijanto Yasa, Ngurah Sedana