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All Journal Buletin Peternakan Jurnal Veteriner Animal Agricultural Journal Jurnal Sain Peternakan Indonesia JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Livestock and Animal Research Animal Production EDUCATIONAL: Jurnal Inovasi Pendidikan dan Pengajaran
Dela Ayu Lestari, Dela Ayu
Program Studi Peternakan, Fakultas Peternakan Dan Pertanian, Universitas Diponegoro, Semarang, 50275
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Economics, Econometrics & Finance Education Veterinary Other

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Journal : Jurnal Veteriner

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In Vitro Ovine Embryo Culture: Effectiveness of TCM-199 in Combination with 17?-Estradiol Hormone and Co-Culture of FTEC Yon Soepri Ondho; Dela Ayu Lestari; Daud Samsudewa
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.646 KB) | DOI: 10.19087/jveteriner.2020.21.4.558

Abstract

Objective of this research was to determine the effectiveness of various culture mediums on thecleavage to morula in in vitro embryo culture of ovine. A total of 1.064 zygotes (fertilized oocytes) were grown in three kinds of different embryo culture medium, which were Tissue Culture Medium 199 (TCM-199) (T1), TCM-199 + 17รข-estradiol (T2) and TCM-199 + co-culture of Fallopian Tube Epithelial Cell (FTEC) (T3). Data were analyzed using a completely randomized design with ANOVA analysis and HonestlySignificant Difference test. The variable observed were the percentage of embryo cleavage and morula. Results showed that T3 had the highest percentage (72.45%) of the cleavage occurred in zygotes cultured in the TCM-199 + FTEC co-culture medium, as well as embryo growth to the morula stage (75.75%). Incontrast, zygotes cultured in T1 and T2 had lower cleavage and lower morula development than T3, as much as 27.38%; 47.82% and 58.15%; 66.66%, respectively. In conclusion, TCM-199 + FTEC co-culture medium is the best medium for embryo culture.
Co-Authors A. Setiaji Agustine, Ananda Dwi Ahmad, Hasan Moftah Ahmed Ahmed, Bilal Akramullah, Muh Asep Setiaji Baharudin Baharudin Daud Samsudewa E. Kurnianto Edjeng Suprijatna Edy Kurnianto Enny Tantini Setiatin Kamalludin, Mamat Hamidi Kamila, Firda Tasya Latifa, Alfa Putri Mustofa, Fatmawati Nabilah, Zata Philco, Syaddad Verahry Saiful Bahri Setiaji, Asep Sutopo Sutopo Sutopo Sutopo Umi Hijriyah Warsono Sarengat Y. S. Ondho Yon Soepri Ondho Yousaf, Muhammad Rizwan YUSNITA, ERNI