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All Journal ACTA VETERINARIA INDONESIANA Jurnal Veteriner Jurnal Peternakan Indonesia ARSHI Veterinary Letters Journal of Parasite Science
Agus Wijaya
Divisi Penyakit Dalam Dan Patologi Klinik, Sekolah Kedokteran Hewan Dan Biomedis, Institut Pertanian Bogor
Agriculture, Biological Sciences & Forestry Immunology & microbiology Veterinary

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Journal : Jurnal Veteriner

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Perbandingan Deteksi Titer Antibodi Pascavaksinasi Rabies Berbasis Kolorimetri Menggunakan ELISA Reader dan Kamera Telepon Genggam Koekoeh Santoso; Ulfatin Khoiriyah Herowati; Dordia Anindita Rotinsulu; Sri Murtini; Muhammad Yusuf Ridwan; Denny Widya Hikman; Abdul Zahid; Ardilasunu Wicaksono; Arifin Budiman Nugraha; Usamah Afiff; Agus Wijaya; Ridi Arif; Ronald Tarigan; Edi Sukmawinata
Jurnal Veteriner Vol 22 No 1 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (250.315 KB) | DOI: 10.19087/jveteriner.2021.22.1.79

Abstract

Rabies is an infectious disease, zoonotic, caused by virus from the genus Lyssa virus and generally transmitted by the bite of rabid animal, especially rabies infected dog. Rabies is preventable but is always fatal to humans if the central nervous system (CNS) is infected. Vaccination has been used as one of rabies prevention programmed. A total of 83 samples were tested using an Indirect ELISA (Enzyme Linked Immunosorbent Assay) to identify post-vaccination rabies antibody titer. Antibody titres correlated with absorbance values and standard solutions concentrations. Absorbance value can be determined using ELISA reader and mobile phone camera. Absorbance were read at 450 nm and 620 nm as reference using ELISA reader and image from mobile phone camera using image processing software (ImageJ). The aim of this study is to compared between ELISA reader as gold standard and mobile phone camera through validity testing such sensitivity, specificity, and accuracy. There is no significant difference between gold standard and alternative test equipment. The mobile phone camera has sensitivity 98,6%, specificity 88.8 % and accuracy 97,5%. The image processing method using ELISA reader is relatively expensive and difficult to hold in laboratory with minimum funds. Image processing method using a mobile phone camera with ImageJ application is expected to be an alternative tool to read the result of ELISA.
Keragaman Endoparasit pada Macaca fascicularis dan Potensi Zoonotiknya dengan Cuaca Berbeda di Kota Kupang (ENDOPARASITIC DIVERSITY IN MACACA FASCICULARIS AND ITS ZOONOTIC POTENTIAL ON DIFFERENT WEATHER IN KUPANG CITY) Jayusman Arsiyanti Joesoef; Dondin Sajuthi; Agus Wijaya; Maxs Urias Ebenhaizar Sanam
Jurnal Veteriner Vol 19 No 4 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (135.564 KB) | DOI: 10.19087/jveteriner.2018.19.4.451

Abstract

Long-tailed macaque (Macaca fascicularis) belongs to least concern categories of animals, and often found in various regions of Indonesia, including Kupang, East Nusa Tenggara. Their habitat are one of the tourist destinations, causing interaction between them and humans. The interaction facilitates the transmission of disease agents, for example endoparasit. This study was conducted to find out the diversity and prevalence of endoparasites in long-tailed macaque, weather effects, and zoonotic potential in Kupang City. Fifty fecal samples were collected and analyzed using formalin ethyl acetate concentration technique (FECT). A total of six endoparasites were recovered including four helmints (hookworm, Strongyloides, Toxocara, and Acantocephala) and two protozoans (Balantidium coli and Entamoeba sp.). The highest prevalence of infection occurred in February (rainfall 302.4 mm) and consisted of hookworm (86%) and B. coli (66%). If rainfall increases, prevalence will also increase. The high prevalence of parasites is not always associated with the appearance of clinical symptoms. An individu diagnosed with parasites if the eggs or cysts have been identified from the faeces. The high prevalence caused by the absence of deworming program. Among the endoparasites that have been found, some of which are potentially zoonotic, such as: Strongyloides sp., Toxocara sp., Acanthocephala, B. coli, and Entamoeba sp.
Co-Authors A. Suprayogi A. Wijaya A.S. Satyaningtijas Abdul Zahid Afiff , Usamah Annisa Madyanti Geminastiti Parampasi Ardilasunu Wicaksono Asah Hilaliah Bambang Purnomo Sunardi Budiarto Budiarto Citra Ayu Lestari D. R. Ekastuti Denny Widaya Lukman Denny Widya Hikman Dondin Sajuthi Edi Sukmawinata Edi Sukmawinata Egga Johar Praptanto Etih Sudarnika F. B. Harlimawan Faisal Amri Satrio Hadi Putra Rihansyah HERA MAHESHWARI Intan Maria Paramita Isdoni Bustaman Jayusman Arsiyanti Joesoef koekoeh santoso Maxs Urias Ebenheizer Sanam Muhammad Elmanaviean Nugraha, Arifin Budiman P. Achmadi R. Tarigan Ridi Arif Risqika Akla Velayati Ronald Tarigan Rotinsulu, Dordia Anindita sri murtini . Ulfatin Khoiriyah Herowati W. Manalu Wahyu Sri Wulandari Yohan Naim Nurul Fatonah Yusuf Ridwan