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Identifikasi Jamur Penyebab Penyakit Kudis pada Buah Jeruk Siam Kintamani (Citrus nobilis L.) dan Pengendaliannya Secara Hayati ATPENIA BR SEMBIRING; I MADE SUDANA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.1, Januari 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Fungi Causing Scabies Disease on Kintamani Siam Orange Fruit (Citrus nobilis L.) and Biological Control Siam orange is one of the leading fruit commodities in the Province of Bali, Kintamani District is one of the areas that produce siam orange in Bali. Orange fruits in Kintamani District show many symptoms of attacks on the skin of orange fruits such as scabies. Symptoms of the attack include brownish to gray spots, slightly protruding like a cork, forming a pattern on the skin of the fruit like a splash of water and rough to the touch like scabies, the cause of this disease is unknown. Orange scab disease get less attention by farmers because it is considered to have no effect on the quantity or taste of orange fruits, but orange scab disease has seriously damaged the appearance of orange fruits. The purpose of this study was to determine the pathogens that causing scurvy in orange fruits and to determine which fungi are antagonistic and able to suppress the growth of pathogens that cause scurvy in Kintamani District. The results of the isolation and molecular identification of the scurvy pathogenic fungi showed that the fungi was Diaporthe phaseolorum and the antagonistic fungi that could control the pathogenic fungi in vitro were Aspergillus niger isolated from healthy citrus fruits in the same location and the Trichoderma fungi group, namely T. asperellum, T. harzianum, T. koningii, T. viride.
Uji Efektivitas Konsentrasi Minyak Atsiri Sereh Dapur (Cymbopogon Citratus (DC.) Stapf) terhadap Pertumbuhan Jamur Aspergillus Sp. secara In Vitro MARIA ULFA ELLA ELLA; KETUT SUMIARTHA SUMIARTHA; NI WAYAN SUNITI; I PUTU SUDIARTA; NYOMAN SEMADI ANTARA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.2, No.1, Januari 2013
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT In Vitro Study of the effectiveness of Essential Oil Extracted from Lemongrass (Cymbopogon citratus) to Aspergillus Sp. Study on determining of the effectivenes of essential oil extracted from lemongrass (Cymbopogon citratus) in order to suppressing the growth of fruit rot fungi (Asoergillus sp.) and also to determining the minimum concentration that able to inhibit colony growth and spore formation of the Aspergillus sp. in vitro, was conducted in Laboratory of Plant Diseases from June into November 2012. The result showed that the fungi were isolated from rot fruit mango has similarities with the fungi Aspergillus sp. The characteristic of Aspergillus has cepted hyphae, growth appear colonies, conidiophores uncepted and stand up, the tip of conidiophore is swollen and produce vesicle form, the surface of vesicle is covered by fialid that usually simple and colored or colorless, fialid produced conidia which were form green, brown or black chain. Essential oil of lemongrass have an antifungal activity and it was found effective to inhibit growth of the fruit rot fungi Aspergillus sp. on PDA. The inhibition zone of the lemongrass essential oil against Aspergillus sp. were 85 mm which is include a very strong category. Effective concentration of essential oil of lemongrass to inhibit the growth of Aspergillus sp. are 0,6% - 1%. The persentase of inhibition in this concentration reached to 100%. On the other hand, the control treatment, 0,2%, and 0,4% concentration were 0%, 0%, and 28,53%, respectively. Minimum concentration which is capable to inhibit the formation of spores and growth of fungi colonies were 0,2% and 0,3% concentration. The compounds suspected as antifungal against Aspergillus sp. were ?-citral (geraniol) and ?-citral (neral). Keywords: Lemongrass (Cymbopogon citratus), essential oil, antifungi, Aspergillus sp.
Pemanfaatan Aeromonas hydrophila Untuk Mengendalikan Penyakit Layu Fusarium Pada Beberapa Varietas Sorgum Manis (Sorghum bicolor (L.) Moench I WAYAN GENTA ARIAWAN; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.4, No.2, April 2015
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The Use of Aeromonas hydrophila to Control Fusarium Wilt Disease on Several Varieties of Sweet Sorghum (Sorghum bicolor (L.) Moench) Objective of this study was to test the effectiveness of A. hydrophila to control wilt disease caused by Fusarium sp. on several varieties of sweet sorghum. Results of this study showed that application of rhizobacteria affected the growth and the yield of sweet sorghum. Under in vitro condition, treatments with A. hydrophila isolate KtBlt2 could suppress the growth of the Fusarium sp. with inhibitory activity 76.06% when compared to control. Under field condition treatment P6 (treatment wilt A. hydrophila on variety FS-501) could suppress Fusarium wilt disease with the lowest disease incidence by 3.24%. It is necessary to test the stability of A. hydrophila as biocontrol agent against Fusarium sp. Keywords : A. hydrophila, Fusarium sp., sweet sorghum
Identifikasi Penyakit Antraknosa Tanaman Jeruk Nipis [Citrus aurantifolia (Christm.) Swingle] di Desa Kertalangu Kecamatan Denpasar Timur I GEDE WIGUNA ARDINATA; I MADE SUDARMA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Leaf Spot Disease of Lime Plants [Citrus aurantifolia (Christm.) Swingle]in Kertalangu Village,District of East Denpasar.In Kertalangu Village, District East of Denpasar there are some plants lime getting anthracnose disease. Disease status is unknown and the plants are not too noticed by their owners. The purpose of this research was to identify the disease that attack lime plants. This research outlines the causes of disease, the extent of damage and the rate of infection of pathogens that attack lime plants. The research was conducted over three months since April until June 2015.This research has shown that the pathogen causing anthracnose disease in lime plants is Colletotrichum gloeosporioides. The disease incidence ranges from 26.46% - 35.87%, pathogen infection rate ranges from 0.0171 to 0.0157 per leaf per day (? 0.11), which means that 1000 leaves, infected 171-157 per day with the criteria of slow infection rates and the extent of damage or the disease severity ranges from 17.31% - 24.47% (> 10% - ? 25%), classified as mild criteria.
Uji Efektivitas Ekstrak Beberapa Jenis Rimpang Jahe (Zingiber officinale Rosc.) Terhadap Patogen Phytophthora palmivora Butl. Penyebab Busuk Buah Kakao MARTINA E SITEPU; NI WAYAN SUNITI; I DEWA PUTU SINGARSA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.8, No.3, Juli 2019
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Effectiveness of Some Ginger Extracts (Zingiber officinale Rosc.) against the Pathogen of Phytophthora palmivora Butl. Causes of Cocoa Fruit Rot The purpose of this study was to determine the effectiveness of some ginger (Zingiber officinale Rosc.) extracts to prevent pathogen of Phytophthora palmivora Butl. causes of cocoa fruit rot. In vitro, the concentrations of 0,1%, 0,2%, 0.25%, 0.5%, 1%, 1.5%, 2%, and 2.5% were tested to determine the Minimum Inhibitory Concentration (MIC) while the extract concentration of 0.25%, 0.5%, 1%, 1.5%, 2%, 2.5% were tested to determine the percentage of inhibitability of Phytophtora palmivora Butl. The concentrations of 0.25%, 0.5%, 1%, 1.5%, 2%, and 2.5% were tested in vivo at the Laboratory to determine the effectiveness of extracting ginger rhizomes in controlling fruit rot in cocoa plants. The results of the in vitro test showed that the MIC of Z. officinale var. officinale rhizome extract was 0,25%, while the MIC of Z. officinale var. rubrum rhizome and Z. officinale var. amarum were 0.1%. The concentration of inhibition of Z.officinale var officinale extract, Z.officinale var rubrum extract and Z.officinale var amarum extract are most effective against the growth of colonies of Phytopthora palmivora Butl. was 2.5% with the inhibition were 48,74%, 72,78%, 79,03%. The result of the in vivo test showed that the Z.officinale var amarum extract effectively suppressing the attack of cocoa rot fruit pathogens marked by the decrease of percentage by 80%.
Uji Daya Hambat Jamur Antagonis terhadap Jamur Penyebab Penyakit Utama pada Tanaman Ubi Jalar Ungu (Ipomoea batatas (L.)) Secara In Vitro NI WAYAN EKA SUDI ARTI; NI WAYAN SUNITI; I DEWA PUTU SINGARSA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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In Vitro InhibitionTest of Antagonistic Fungi on Pathogenic Fungi of the Main Diseases in Purple Sweet Potatoes (Ipomoea batatas (L.) Purple sweet potato is a plant commodity that is in great demand by people today. Peringsari Village, Selat Sub-district, Karangasem Regency is one of the sweet potato-producing areas in Bali. The purple sweet potato plant developed in Peringsari village shows many symptoms of the disease on the stems and leaves of the plant. The cause of the main disease of this plant is not yet known, but the symptoms shown are such as attack by pathogenic fungi. This purple sweet potato disease does not get much attention from farmers because it is considered to have no effect in terms of the quantity and quality of sweet potatoes. The purpose of this study was to determine the main pathogens that cause disease in purple sweet potato plants and to determine which antagonistic fungi are effective in suppressing the growth of pathogens in purple sweet potato plants in Peringsari Village, Selat District, Karangasem Regency. This research was conducted in Peringsari Village, Selat Subdistrict, Karangasem Regency for sampling to be continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. Field observations, isolation, and morphological identification showed that the main disease-causing pathogen in purple sweet potato was the fungus Fusarium sp. and the antagonistic fungi that can control pathogenic fungi in vitro are Trichoderma asperilum, Trichoderma harzianum, Trichoderma koningii, and Trichoderma viride.
Potensi Jamur Asal Rizosfer Tanaman Cabai Rawit (Capsicum frutescens l.) Sehat dari Desa Bumbungan Kecamatan Banjarangkan Kabupaten Klungkung dalam Upaya Mengendalikan Penyakit Layu Fusarium secara In Vitro FERDIANSYAH DWI MAULANA; I MADE SUDARMA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.5, No.2, April 2016
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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The potential of rhizosphere fungi from health cayenne pepper plant (Capsicum frutescens L.) from Bumbungan Village to control the wilt disease Fusarium in vitroThe objectives of the research were to find out the potential and level domination (prevalance) of rhizosphere fungi from health cayenne pepper plant (Capsicum frutescens L.) to control the wilt disease Fusarium. It was done by using the in vitro inhibition test of rhizophere fungi againts the Fusarium oxysporum f.sp. capsici with dual culture technique. The study included 1) rhizosphere soil sampling of health cayenne pepper plants; 2) isolation and identification of soil fungi; 3) isolation of the pathogen Fusarium oxysporum f.sp capsici; 4) purification of pathogenic isolates of Fusarium oxysporum f.sp capsici; 5) prevalence of fungal isolates; 6) testing the inhibition of rhizosphere fungi on the growth of the fungus Fusarium oxysporum f.sp. capsici. The result showed that the identified rhizosphere fungi were the genus of Aspergillus spp., Penicillium spp., Trichoderma spp. The prevalence of fungi in the rhizosphere that dominate the healthy cayenne pepper plant is the genus of Penicillium spp. and Aspergillus spp. which have the same prevalence rate of each, 42.1 %. while the fungus Trichoderma spp. has prevalence rate 15,8 % . The results showed that all the rhizosphere fungi have potential to control wilt disease Fusarium oxysporum f.sp. capsici with different levels of inhibition. Based on the results of this study, further research needs to be done in order to apply directly the identified antagonist fungi to plant that already affected with wilt diseas
Identifikasi Jamur Patogen Penyebab Penyakit Layu Pucuk pada Tanaman Adenium spp. di Kota Denpasar dan Potensi Pengendaliannya dengan Jamur Antagonis DWI SUGIARTA; I PUTU SUDIARTA; NI WAYAN SUNITI; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.3, Juli 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Pathogenic Fungi that cause Wilting Diseases on Adenium spp. Shoot in Denpasar City and the Potential Control with Antagonistic Fungi Ornamental plants of Adenium, known as kamboja Jepang have various types of flowers and stem shapes and have become unique ornamental plants in Indonesia. Adenium plants that grow in Denpasar City showed the symptoms of pathogenic fungi like wilting on the shoots. This study aims to identify the pathogenic fungi that cause major diseases in Adenium spp. in the area of ??Denpasar City. The activities carried out in this study were (1) sampling, (2) isolation of the pathogenic fungi from symptomatic Adenium plant, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) inhibition test of the antagonistic fungi against the pathogenic fungi. This research was conducted in Denpasar City and the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. The results of this study were successful in identifying Fusarium sp. as a pathogen that causes wilting of the shoots or dieback. The potential for biological control by utilizing antagonistic fungi showed that Gliocladium sp. can suppress the growth of pathogenic fungi Fusarium sp. up to > 87% and Trichoderma sp. up to > 88 %.
Identification of Endophytic Fungi from Rice Grain and It’s Inhibiting Ability by In Vitro Against Pyricularia oryzae Cav. NI PUTU LINDA SUNARIASIH; I KETUT SUADA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.3, No.2, April 2014
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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ABSTRACT Identification of Endophytic Fungi from Rice Grain and It’s Inhibiting Ability by In Vitro Against Pyricularia oryzae Cav. Fungal endophyte is a fungi that lives in the tissues of plants without causing disease symptoms on host plants. During symbiotic, many endophyte could produce antibiotic compounds, those can make endophyte microbes become biological control agents against pests and plant diseases. The aims of this study is to determine the types and the number of endophytic fungi spesies that exist in rice seeds stadia, also growth inhibitory ability against Pyricularia oryzae Cav. in vitro. Based on microscopic identification and DNA analysis were found 14 species of endophytic fungi. Endophytic fungi were able to inhibit the develop of Pyricularia oryzae Cav. amount. The greatest inhibitor was Unidentified-2 isolates of 65.6% followed by fungi Phaeosphaeriopsis musae, Sarocladium oryzae, and Sordariomycetes sp., with inhibition of 63.3%, 61.1%, and 58.9% respectively. Key words: endophytic fungi, inhibition, Pyricularia oryzae, rice,  In vitro.
Identifikasi Morfologi Jamur Kontaminan pada Naskah Lontar I KOMANG VIDIA DHARMA TARO PUTRA; I PUTU SUDIARTA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.10, No.4, Oktober 2021
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Morphological Identification of Fungal Contaminant in Lontar Manuscripts The lontar manuscript is a cultural heritage of Balinese ancestors that contains knowledge that includes mantras, traditional medicine, songs and religious history. The physical condition of the lontar manuscript is very susceptible to being damaged by fungi. The fungus that grows on lontar manuscripts is thought to be of the species: Fusarium, Penicillium, and Aspergillus flavus. The purpose of this study was to determine the type of fungus that attacks lontar palm. The study began with sterilization of the instrument, manufacture of PDA media, isolation of pathogenic fungi, Koch's postulate test on palm lontar manuscripts, identification of morphology. The results showed that the fungus contained in the lontar manuscript is a type of fungus Penicillium sp. it can be seen from the identification results that the fungus has the following characteristics: white and brownish orange colonies, has insulated hyphae, erect and insulated conidiophores, conidia are chain-shaped and congregate on top of phialids.