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All Journal Agrotrop : Journal on Agriculture Science AGRITROP International Journal of Biosciences and Biotechnology Buletin Udayana Mengabdi E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Journal of Comprehensive Science Nandur
NI WAYAN SUNITI
Program Studi Agroekoteknologi Fakultas Pertanian Universitas Udayana, Bali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Languange, Linguistic, Communication & Media Library & Information Science

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Pengaruh Aplikasi Pseudomonas fluorescens dan Trichoderma spp. terhadap Penyakit Akar Gada serta Pertumbuhan Tanaman Kubis (Brassica oleracea L.) di Desa Candikuning, Tabanan, Bali I KADEK NGESTIKA PRADNYANA; NI WAYAN SUNITI; I KETUT SUADA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.7, No.4, Oktober 2018
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

The effect of Pseudomonas fluorescens and Trichoderma spp. Applications against Clubroot Disease and Growth of Cabbage (Brassica oleracea L.) in Candikuning Village, Tabanan, Bali The research was aimed to find the effectiveness of P. fluorescens and Trichoderma spp. in suppressing clubroot disease caused by P. brassicae Wor. and increasing the growth of cabbage plants as well. The microbes treatments were set on randomized block design, consisting of 36 treatments and 3 replications. The variables observed were percentage of attack, number of clubroot, microbial density, plant height, leaf area, chlorophyll contents, and plant weight,. The results showed that the combination of P. fluorescens and Trichoderma spp. was able to suppress the percentage of pathogen attack and increase the growth of cabbage plants as well. The most effective treatment was combination between P. fluorescens-1 and Trichoderma-4, followed by combination treatment of P. fluorescens-1 and Trichoderma-3 and then P. fluorescens-1 and Trichoderma-5.
Keragaman dan Daya Hambat Spora Tular Udara yang Mengkontaminasi Media Baglog Jamur Tiram (Pleurotus ostreatus (Jacq. ex Rr) Kummer) I MADE SUDARMA; NI MADE PUSPAWATI; NI NENGAH DARMIATI; KETUT AYU YULIADHI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS; I NYOMAN WIJAYA; DWI WIDANINGSIH
Agrotrop : Journal on Agriculture Science Vol 5 No 2 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Diveristy and Inhibition of Air-Borne Spores That Contaminate Substrat of OysterMushroom (Pleurotus ostreatus (Jacq. Ex Fr) Kummer). The aim of research to know thediversity, prevalence and inhibition of air-borne spores that could potentially contaminatesubtsrat baglog of oyster mushrooms. In this study using the method: (1) the arrest of sporescarried out during the hours of 7 am to 13 pm by placing three petri dish that already containsmedia PDA 3 pieces, and repeated 3 times, (2) inhibition ability of air-borne spores againstoyster mushroom. The place and time of the study conducted at Jl. Siulan Gang Zella No. 7Denpasar, and implemented in October 2014 to February 2015. The results showed that 13genera, including Aspergillus spp, as many as 10 with a prevalence of 13.51%, Aspergillusniger 7 (9.45%), Brachysporium sp., 1 (1.35%) , Cunninghamella sp. 1 (1.35 %), Fusariumspp. as many as 19 (25.6 %), Giotrichum sp. 1 (1.35%), Mucor spp. , a total of 18 (24.32%),Neurospora spp., as many as 8 (10.81%), Penicillium spp. 2 (2.70%), Phytophthora spp. 4(5.40%), Stachybotrys sp. 1 (1.35%), Trichoderma sp. 1 (1.35%) and Umbelopsis sp. 1 (1.35%). The highest prevalence achieved by Fusarium spp . amounting to 25.67 %, followed byMucor spp. 24.32%. Diversity index (H') was obtained from each replicate of 0.6438, 0.7048,0.5611 with a 0.6366 average. Dominance index (C) obtained by 0.6331. 0.8646, 0.6146, andaverage 0.7041. Diversity including < 1, marked by low diversity and dominance index closeto 1, meaning there is dominance. The dominance held by Fusarium spp . and Mucor spp.Inhibitory effects of air -borne fungi on the growth Oyster mushroom in vitro; The highestachieved by Fusarium spp, with inhibition of 94.00 ± 1.2%, followed by Aspergillus spp.amounting to 92.15 ± 1.5%, and the lowest was achieved by Penicillium spp . amounting to70.37 ± 2.5%.
Status Penyakit Layu pada Tanaman Cabai Rawit (Capsicum Frutescens L.) di Banjarangkan, Klungkung I MADE SUDARMA; NI MADE PUSPAWATI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS
Agrotrop : Journal on Agriculture Science Vol 4 No 2 (2014)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Status of wilt disease in pepper (Capsicum frutescens L.) at Banjarangkan, Klungkung. Wilt disease in pepper , has led to a total yield loss in Banjarangkan, Klungkung regency. Until now the disease was still a threat to farmers in the area pepper. The purpose of research to study the disease includes symptoms, causes disease, the percentage of the disease and the rate of infection (r). Research using existing observations of the disease in three plots of local farmers. Each plot the observed number of diseased plants and entire plants. Samples of diseased plants put in a plastic bag, then placed in an ice box , to be observed macroscopically in the laboratory. The study was conducted in two places, namely surveys Banjarangkan disease in Klungkung , and isolation of the pathogen as well as pathogenicity test carried out in the Laboratory of Plant Pathology, Faculty of Agriculture, Udayana University, Jl. PB Sudirman Denpasar-Bali. The research was conducted from June to November 2013. The results showed that the pathogen that causes wilt disease in pepper at Banjarangkan, Klungkung regency was the fungus Fusarium oxysporum f.sp. capsici, with the disease incidence was 76.67%. Fusarium wilt disease epidemic in pepper indicated by the rate of infection, first gained 0.44 per unit per day, then decreases with time, 0.23, 0.12 and 0.11 per unit per day respectively. The rate of infection ranged from 0.11 to 0.44 per unit per day, this means that the moderate criteria.
Uji Daya Hambat Jamur Endofit dan Eksofit dalam Menekan Pertumbuhan Fusarium oxysporum f.sp. vanillae Penyebab Busuk Batang Panili Secara In Vitro NI WAYAN SUNITI; I MADE SUDARMA
Agrotrop : Journal on Agriculture Science Vol 6 No 2 (2016)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

Study on In Vitro Inhibitory Ability of Endophytic and Exophytic Fungusin Suppressing the Growth of Fusarium oxysporum f.sp. vanillae thatCauses Stem Rot of Vanilla. Vanilla stem rot disease caused by Fusarium oxysporumf.sp. vanillae , is still a very dangerous disease and feared by vanilla farmers. Disease until thepresent time there does not yet appear adequate control strategies for the disease. On the basisof these problems interested studied the use of endophytic fungi and exophytic existing on theleaves and stems of healthy plants , which have potential as a biological agent to controlpathogens . The study was conducted in three stages: (1 ) isolation of the pathogen and fungalendophyte and exophytic, (2) identification of pathogenic microscopic morphology, fungalendophyte and exophytic, and (3) test the inhibition of fungal endophyte and exophyticagainst pathogens. Fungi are found as the leaves are Aspergillus niger and Rhizopus spp., Onexophytic on the trunk is Trichoderma sp . and Fusarium spp., as a leaf endophytic found A.niger and Neurospora spp. and as endophytic rod is Neurospora spp . The highest prevalenceachieved by Neurospora spp . which is equal to 100 % on endophyte stem, while Rhizopusspp., and Fusarium spp. by 90 % respectively, exophytic on the leaves and stems. The testresults found that the inhibition of leaf eksofit Rhisopus spp . give up inhibition ranged from70.37±3.2% - 100% , while A. niger amounted to 72.22% . In exophytic on rod found onlyTrichoderma sp. inhibit pathogens by 73.70±3.57%. In endophytic on leaf found A. niger at70.37±3.2% , while Neorospora spp. ranging from 79.11±3.21% - 88.50±2.10%. In theendophytic on trunk was found Neurosporas spp. amounting to 70.74±3.57% - 79.26±1.28%.
Pengendalian Getah Kuning Manggis Menggunakan Ekstrak Rumput Laut dan Pemupukan Kalium I KETUT SUADA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 5 No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The Control of Mangosteen Yellow Latex Using Seaweed Extract and Potassium Fertilizer. The yellow latex disease decreases fruit quality of mangosteen because of exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste, then it does not deserve to be consumed. The causal agent of the disease was Verticillium alboatrum, Fusarium oxysporum, and Pestalotia macrotricha. Control using synthetic chemical for the fungus is severely decreasing the quality of the environment, therefore the use of organic agent such as botanical fungicide and the use of potassium fertilizer is recommended. The purpose of this research is to find the dose of potassium fertilizer and sea weed extract to decrease yellow latex disease of mangosteen fruit. The research was done in-vivo in the field with indicator of the decrease of yellow latex incident. The research showed that potassium fertilizer of 1.5 kg/plant combine with 1% extract was the best treatment with the lowest yellow latex incident of 9.4%. The treatment effective decreasing the disease of 56.3% compared to control and 56.9% when it is compared to 0,2% Antracol fungicide.
Uji In-Vitro Daya Hambat Ekstrak Beberapa Jenis Tanaman terhadap Pertumbuhan Jamur Patogen Getah Kuning Buah Manggis I KETUT SUADA; NI WAYAN SUNITI; NI PUTU LINDA SUNARIASIH
Agrotrop : Journal on Agriculture Science Vol 3 No 2 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

In-vitro Testing of Inhibiting Ability of Some Plants Extractto the Growth of MangosteenYellow Latex Pathogens. The yellow latex disease decreases fruit quality of mangosteen because ofthe exogenic latex causing dirty the fruit, and the endogenic latex makes the bitter fruit taste, thereforethe fruit does not deserve to be consumed. The causal agent of the disease are fungus i.e.Verticilliumalbo-atrum, Fusarium oxysporum,andPestalotia macrotricha, therefore the research was aimedto find the plant extract that could act as antifungal agent. Screening on many kinds of plant extractthrough well difusion method were found that crude water extract of 10% concentration of kayusugih, kayu tulak, sereh dapur, beluntas, puring, sirih hutan, temen, and dapdap were only effectiveto suppress Verticillium albo-atrum.Methanolic plant extract of 10% of endongplant was only effectiveagainst Fusarium oxysporum, while the extract that effective against Pestalotia macrotricha wasnot found yet.
Kultur In-vitro Anggur Laut (Caulerpa lentilifera) dan Identifikasi Jenis Mikroba yang Berasosiasi NI WAYAN SUNITI; I KETUT SUADA
Agrotrop : Journal on Agriculture Science Vol 2 No 1 (2012)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

This research aims to find the best condition for the cultivation of fragment of Caulerpa lentilifera, to determine the factors that influence its growth in-vitro, and to identify the types of microbes associated. The study was conducted at the Marine Tech Laboratory and Marine Biology Laboratory of Udayana University. The study begins with the in-vitro culture of C. lentilifera seaweed at the incubator and the tank. The observations of the morphology and the seaweed growth to determine the suitable conditions, the influens of growth factors and microscopic observation to determine the types of microbes were associated. The best results in in-vitro culture in incubator was achieved by provision of 2 ml of PES in 500 ml of sea water, 5000 lux of light intensity, 12 hours irradiation and 100% medium replacement once a week. Best results in-vitro culture in the using net method was achieved by provision of 3500 lux light intensity, 12 hours irradiation and 70% culture replacement once a week. The microbes associated with the C. lentilifera came from the protozoa phylum, flagellate, and cilliata classes.
Komparasi Laju Pertumbuhan Miselium Jamur Tiram Putih (Pleurotus ostreatus (Jacq. ex Fr) Kummer) pada Komposisi Media Bibit (F3) dan Baglog yang Berbeda I MADE SUDARMA; GEDE WIJANA; NI MADE PUSPAWATI; NI WAYAN SUNITI; I GUSTI NGURAH BAGUS
Agrotrop : Journal on Agriculture Science Vol 3 No 2 (2013)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The growth rate comparison of white oyster mushroom (Pleurotus ostreatus (Jacq. ex Fr)Kummer) mycelium in the composition of different seed (F3) and baglog media . Cultivation ofoyster mushroom (Pleurotus ostreatus (Jacq. ex Fr) Kummer) has grown rapidly along with the increasein income and health awareness. Oyster mushrooms growing need for media with a particular compositionin order to grow optimally. Oyster mushroom production is determined by the quality of the seeds (F3)is used, which is sourced from the media with good quality and composition. The research aimed todetermine the rate of growth of white oyster mushroom mycelium in the different composition of seedmedium (F3) (sawdust: fine bran: corn flour: CaCO3 ). The experiments was conducted at nurseriesand oyster mushroom development, Jl. Siulan Gang Zella No. 7 Denpasar, from June to August 2013.Each treatment contained 50 bottles, and 10 bottles only used as a sample, in environmental conditionswith temperature and humidity ranges, 20-29oC and 59-86% respectively . T-test was used todifferentiate the growth rate of white oyster mushroom mycelium with different compositions. Theresults showed that seeds (F3) derived from the growing media composition, sawdust (1 week old):fine bran: corn flour: CaCO3 (10:4:2:0,5) significantly different and better than the composition sawdust(age 1 month ): fine bran: corn flour (20:2:1:0.5), with a growth rate of mycelium in a mean 6.14±0.56cm/week and 1,81±0,82 cm/week, respectively. Spawn running in baglog with media composition10:4:2:0.5 was 2.77±1.22cm/week, but with composition media 20:2:1:0.5 mycelium could not grow.Effect of temperature and humidity on the growth rate of white oyster mushroom mycelium in seedmedia (F3) is not significantly.
Potensi Bakteri Endofit dari Batang Panili Sehat sebagai Agen Pengendali Hayati Fusarium oxusporum f. sp. vanillae Penyebab Busuk Batang Panili NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 5 No 1 (2015)
Publisher : Fakultas Pertanian Universitas Udayana

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Abstract

The potential of Endophytic Bacteria from Shoot of healthy Vanilla for Biological Control Agenst of Fuxarium oxusporum f. sp. vanilla. The aim of the research was to determine in-vitro inhibitory of endophytic bacteria from healthy stem of vanilla against stem rot dideases caused by Fusarium oxysporum f.sp. vanillae The research was conducted in Phythopatology Laboratory and green house Fakulty of Agiculure from January to August 2014. The results showed that 50 isolates of endophytic bacteria can be isolated from Tabanan and Badung regency. Antagonistic test results from 50 isolate of endophytic bacteria found the six isolates of endophytic bacteria has potential as antagonists against Fusarium oxysporum f.sp. vanillae namely Endophytic Tabanan 1 (T1), T7, T13, Endophytic Badung 28(B28), B30 and B41. In-vitro test showed endophytic bacteria isolate T1 has the highestinhibition to the F. oxysporum f.sp. vanillae growth by 87%. Results of the research in Greenhouse, endophytic bacteria isolate T1 can stimulate the growth of shoots and capable of protecting plants from vanilla stem rot diseases with the lowest attack percentage of 32%.
ISOLASI DAN IDENTIFIKASI PATOGEN GETAH KUNING MANGGIS MELALUI PENDEKATAN POSTULAT KOCH DAN ANALISIS SECARA MOLEKULER I Ketut Suada; Ni Wayan Suniti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

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Abstract

Isolation and identification of mangosteen yellow latex pathogen through Koch’s Postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease caused phusilogical disorder so that cells break due to unstability of cell turgor, the drift irrigation treatment was able to decrease the disease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogens with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species.  The result of the research proved that there were three fungus species as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity ( 74.87%), followed by Fusarium oxysporum ( 70.15%), and Pestalotia macrotricha (20.32%).
Co-Authors Abd. Rasyid Syamsuri ALPIN JUAN HAGATA PINEM ATPENIA BR SEMBIRING BERTA BR GINTING Dewa Ngurah Suprapta DWI SUGIARTA DWI WIDANINGSIH Eaggeliony, Jenifer Christy FERDIANSYAH DWI MAULANA GEDE WIJANA GUSTI AYU DWITA ANDRAWINA Gusti Ngurah Alit Susanta Wirya I DEWA PUTU SINGARSA I DEWA PUTU SINGARSA, I DEWA PUTU I GEDE WIGUNA ARDINATA I Gusti Made Wira Kesuma Jaya I Gusti Ngurah Bagus I KADEK NGESTIKA PRADNYANA I Ketut Suada I KETUT SUMIARTHA I KOMANG VIDIA DHARMA TARO PUTRA I Made Adhika I MADE SUDANA I Made Sudana I MADE SUDANA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUDARMA I MADE SUPARTHA UTAMA I MADE TEDI MAHADI PUTRA I NYOMAN WIJAYA I Putu Sudiarta I PUTU WIRYA SUPUTRA I WAYAN GENTA ARIAWAN I WAYAN RUSMAN IDA AYU GEDE DIANGGI ADIATHY IDA AYU ISTRI MAYADIANTI KETUT AYU YULIADHI Khamdan Khalimi KRISNA SANUBARI PURBA Lombu, Benvan Eliezer Lombu MARIA ULFA ELLA ELLA MARTINA E SITEPU Ni Luh Gede Astariyani NI LUH WAHYU SUTARINI, NI LUH WAHYU Ni Made Puspawati NI NENGAH DARMIATI Ni Nengah Soniari NI PUTU LINDA SUNARIASIH NI PUTU LINDA SUNARIASIH NI WAYAN EKA SUDI ARTI Ni Wayan Siti NOVA SULIYANTI SIANTURI NYOMAN SEMADI ANTARA PANDE WAYAN OKTARDITYA SUDARMA TRISNA AGUNG PHABIOLA Unedo, Dion Yundari, Yundari