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ISOLASI DAN IDENTIFIKASI PATOGEN GETAH KUNING MANGGIS MELALUI PENDEKATAN POSTULAT KOCH DAN ANALISIS SECARA MOLEKULER I Ketut Suada; Ni Wayan Suniti
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 14 No. 2 (2014): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.188 KB) | DOI: 10.23960/j.hptt.214142-151

Abstract

Isolation and identification of mangosteen yellow latex pathogen through Koch’s Postulate application and molecular analysis. The yellow latex disease decreases fruit quality of mangosteen because exogenic latex causes dirty fruit and the endogenic latex makes the bitter fruit taste and does not deserve to be consumed. The causal agent of the disease caused phusilogical disorder so that cells break due to unstability of cell turgor, the drift irrigation treatment was able to decrease the disease of 35.22%, therefore the causal agent could be microbe that promote the yellow latex. This research was aimed to find the yellow latex pathogens with its characteristics. The Koch Postulate was apllied to isolate and proved the microbes associated to the yellow latex and molecular analysis was constructed subsequently to find the microbe species.  The result of the research proved that there were three fungus species as the biotic agent responsible to the disease. The fungus were Verticillium albo-atrum with the highest pathogenecity ( 74.87%), followed by Fusarium oxysporum ( 70.15%), and Pestalotia macrotricha (20.32%).
Uji Efektivitas Ekstrak Buah Andaliman (Zanthoxylum acanthopodium. D.C) Terhadap Phytophthora palmivora Penyebab Penyakit Busuk Buah Kakao (Theobroma cacao L.) BERTA BR GINTING; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 12 No 1 (2022)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2022.v12.i01.p08

Abstract

Effectiveness Test of Andaliman Fruit Extract (Zanthoxylum acanthopodium. D.C) Against Phytophthora palmivora the Cause of Cocoa (Theobroma cacao L.) pod rot disease. Fruit rot disease caused by the fungus Phytophthora palmivora causes the loss of crop yields up to 90%. Therefore, an effort is needed to control Phytophthora palmivora that causes blight in cocoa plantations. Alternative that can be done is the use of botanical fungicides. One of the plants that can be used as a botanical fungicide is the fruit of Andaliman (Zanthoxylum acanthopodium D.C). This study aims to test the effectiveness of andaliman fruit extract against P. palmivora which causes pod rot disease. Colony test and cocoa pod damage test in vivo using extract concentrations, namely 50%; 25%; 16.7%; and 0% (control). The results showed that andaliman fruit extract could inhibit the growth of P. palmivora which can be used as botanical fungicide. The concentration of andaliman fruit extract 50% and 25% can reduce cocoa pod damage caused by P. palmivora by 58% and 45.7%, respectively.
MENATA AGROWISATA “KAMPUNG WISATA GULA AREN” DESA BELIMBING KECAMATAN PUPUAN TABANAN DI MASA PANDEMI Ni Nengah Soniari; I Made Adhika; Ni Wayan Suniti; Ni Wayan Siti; Ni Luh Gede Astariyani
Buletin Udayana Mengabdi Vol 20 No 2 (2021): Buletin Udayana Mengabdi
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.24 KB) | DOI: 10.24843/BUM.2021.v20.i02.p13

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Desa Belimbing merupakan sebuah desa di Kecamatan Pupuan Kabupaten Tabanan kaya akan potensi wisata alam dan tradisi masyarakat namun belum dikelola secara optimal. Untuk memacu perkembangan pariwisata desa ini, pemerintah desa dan Pemda Tabanan mengembangkan wisata dengan brand “Kampung Wisata Gula Aren”. Tematik ini diambil dari produk unggulan desa berupa gula aren dan tradisi pengolahannya yang sangat menarik sebagai atraksi wisata minat khusus. Wisatawan yang berminat belajar cara membuat olahan dari nira aren dapat berkunjung ke desa Belimbing sambil menikmati keindahan alam perdesaan yang masih alami. Tim dari Program Pengembangan Desa Mitra (PPDM) secara bertahap melakukan penataan lokasi dan spot atraksi serta menyiapkan sarana pendukung lainnya untuk menyambut wisatawan di era New Normal . Tahapan kegiatan yang dilakukan adalah pendampingan kelompok tani gula aren dalam meningkatkan kualitas dan kuantitas produksinya ; menentukan spot atraksi pengolahan gula aren; edukasi pokdarwis dan kelompok petani gula aren dan masyarakat lainnya untuk menerapkan protokol kesehatan di masa pandemi serta melakukan pendampingan pembuatan PERDES mengenai agrowisata di desa Belimbing. Tujuan akhirnya adalah masyarakat berdaya dari segi ekonomi dan tetap eksis menghadapi kondisi perubahan tatanan kehidupan baru.
Eksplorasi Agens Hayati Rizoplan Sebagai Pengendali Jamur Fusarium oxysporum f.sp. cubense I Gusti Made Wira Kesuma Jaya; Khamdan Khalimi; Ni Wayan Suniti
Nandur Vol 2 No 1 (2022)
Publisher : Fakultas Pertanian, Universitas Udayana

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Abstract

Fusarium wild or wilt disease that attacks banana plants is caused by the pathogenic fungus Fusarium oxysporum f.sp. cubense This disease is difficult to control because it lasts a long time in the soil by forming chlamydospores. Currently, the control of this disease is still using synthetic fungicides, but this method can cause damage to the ecosystem. Biopesticide is one of the environmentally friendly control of plant pathogens because biopesticides use biological agents. The purpose of this study was to export rhizoplan bacteria from banana plant roots which have antifungal activity against F. oxysporum f.sp. cubense. The results showed that rhizoplan bacteria were able to inhibit the growth of Fusarium oxysporum f.sp. cubense fungal colonies on potato dextrose agar (PDA) media with an inhibitory percentage of 90.90% when compared to the control. The results of the bacterial filtrate test of rhizoplan at a concentration of 50% were able to inhibit the growth of the fungus Fusarium oxysporum f.sp. cubense with an inhibitory percentage of 92.72%.
Identifikasi Penyebab Penyakit Bercak Daun Mycosphaerella pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis secara In Vitro GUSTI AYU DWITA ANDRAWINA; GUSTI NGURAH ALIT SUSANTA WIRYA; NI WAYAN SUNITI; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Identification of Pathogenic Fungi causes Mycosphaerella Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a fruit cultivation plant that develops in Indonesia and has attractiveness and high economic value. Strawberry plants that grow at the center of strawberry production in Bali show symptoms of pathogenic fungus attack. Symptoms caused by the presence of brownish purple spots with a white center on the leaves. The purpose of this study was to identify pathogenic fungi that caused Mycosphaerella leaf spot disease on strawberry plants located at the center of strawberry production in Bali. The activities carried out in this study were (1) sampling and calculating the percentage of Mycosphaerella leaf spot disease on strawberry plants, (2) isolation of pathogenic fungi from symptomatic leaves of strawberry plants, (3) pathogenicity testing, (4) identification of pathogenic fungi, and (5) test the potential inhibition of antagonistic fungi against pathogenic fungi. This research was conducted in Candi Kuning and Br Kembang Merta Village, Tabanan Regency and Pancasari Village, Buleleng Regency, and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University. The results of this study were successful in identifying Mycosphaerella fragariae as a pathogen causing leaf spot disease on strawberry plants in Bali and the potential for biological control by utilizing antagonistic fungi in vitro showed that Trichoderma viride, Trichoderma asperellum, Gliocladium sp. can suppress the growth of pathogenic fungi in strawberry plants of > 90%, Trichoderma harzianum and Trichoderma koningii up to >80%.
Uji Efektivitas Ekstrak Daun Awar-Awar (Ficus Septica Burm F) untuk Menghambat Pertumbuhan Jamur Phytophthora infestans Penyebab Penyakit Hawar Daun Tomat NOVA SULIYANTI SIANTURI; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 13 No 1 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i01.p05

Abstract

Effectiveness of Awar-Awar (Ficus septica Burm F) Leaf Extract to Inhibit the Growth of the Fungus Phytophthora infestans that Causes Tomato Late Blight. Tomato late blight caused by the fungus Phytophthora infestans is one of the main diseases affecting tomato production in the world. Therefore, it needs to be controlled in an environmentally friendly way, one of which is by using vegetable pesticides. Awar-awar leaves are one of the plants whose extracts can be used as vegetable fungicides. This study aimed to examine the potency of awar-awar leaf extract against P. infestans which causes late blight of tomatoes. MIC test and colony test used 10 extract concentrations, namely 0.1%; 0.2%; 0.3%; 0.4%; 0.5%; 0.6%; 0.7%: 0.8%; 0.9%; 1.0% and control. The in vivo test used 8 extract concentrations, namely 0.3%; 0.4%; 0.5%; 0.6%; 0.7%: 0.8%; 0.9%; 1.0% and control. The results showed that the Minimum Inhibitory Concentration (MIC) of awar-awar leaf extract to suppress the growth of P. infestans was 0.3%, which means that it is suitable for use as a vegetable pesticide. Colony test results showed that awar-awar leaf extract was able to inhibit the growth of P. infestans fungal colonies. In vivo awar-awar leaf extract inhibits the growth of fungal colonies and the occurrence of infection, and can be fungicidal to suppress the growth of P. infestans. Extract concentration of 1% can suppress tomato leaf damage by P. infestans by 75.90%.
Uji Efektivitas Ekstrak Daun Trembesi (Samanea saman (Jacq.) Merr) Untuk Menghambat Pertubuhan Jamur Colletotrichum sp. Penyebab Penyakit Antraknosa Pada Pepaya (Carica Papaya) ALPIN JUAN HAGATA PINEM; DEWA NGURAH SUPRAPTA; NI WAYAN SUNITI
Agrotrop : Journal on Agriculture Science Vol 13 No 2 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i02.p05

Abstract

Effectiveness of Rain Tree Leaf Extract (Samanea Saman (Jacq.) Merr) to Inhibit the Growth of the Fungus Colletotrichum sp. the Cause of Anthracnose Disease on Papaya (Carica papaya). Due to its high nutritional value and high economic value, papaya is a local fruit that is in great demand in the community. The production of papaya fruit fluctuates due to a number of factors, one of which is the fungus Colletotrichum sp., which causes anthracnose disease in papaya fruit. In most cases, synthetic fungicides are utilized to combat anthracnose. Reduce the use of synthetic fungicides and replace them with more environmentally friendly plant-based fungicides, such as those derived from plant extracts. This study aimed to determine the efficacy of rain tree (Samanea saman) leaf extract in inhibiting and controlling the growth of the anthracnose-causing fungus Colletotrichum sp. in papaya fruit. In vitro and in vivo evaluation of the efficacy of trembesi leaf extract against the fungus Colletotrichum sp. The minimum inhibitory concentration (MIC) of trembesi leaf extract to inhibit the growth of Colletotrichum sp. was determined to be 0,1%. The results of the colony test demonstrated that the trembesi leaf extract inhibited the growth of Colletotrichum sp. colonies. In vivo inhibition tests revealed that trembesi leaf extract could inhibit the growth of fungal colonies, prevent infection, and suppress the growth of Colletotrichum sp. The 2,5% extract concentration can prevent 66,22% of the damage caused by Colletotrichum sp. to papaya fruit.
Pengendalian Penyakit Kudis (Diaporthe phaseolorum C&E.) Buah Jeruk Siam (Citrus nobilis L.) Secara Hayati, Nabati dan Mekanik PANDE WAYAN OKTARDITYA SUDARMA; I MADE SUDANA; NI WAYAN SUNITI
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.12, No.1, Januari 2023
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Abstract

Utilization of Biologic, Biopesticide and Mechanic to Control Scabies Disease Pathogen (Diaporthe phaseolorum C&E.) Growth on Siam Orange Fruit (Citrus nobilis L.) Siam orange is the main commodity from Bali, more precisely located in Gianyar, Bali. The disease that most attacks the Siam orange is scab caused by Diaporthe phaseolorum. Scab attacks the skin of orange fruits shows symptoms that look like spots, brown to gray, slightly protruding like a cork, and rough texture. Treatment by spraying antagonist agents once a week. The type of antagonist used was 3 antagonist microbes, Balinese spice solution, and mechanical treatment using plastic. Aspergillus aculeatus treatment, namely the use of the fungus Aspergillus aculeatus, was the most effective in suppressing the growth of scabs on Siam orange plants. AL treatment succeeded in suppressing the scab pathogen well. The mean of Aspergillus aculeatus treatment is the smallest in the percentage of disease on tree, which is 60%, and the mean intensity of AL disease is the smallest, which is 28.14%. Control using natural enemies is very influential in suppressing the growth of scab pathogens in siam orange fruit caused by D. phaseolorum. The most effective treatment in suppressing the intensity of pathogenic disease-causing scabs in Siam oranges is AL treatment using the antagonist fungus Aspergillus aculeatus which can suppress the deseases of scab up to 28.14%.
Efektivitas Rizobakteri untuk Menghambat Pertumbuhan Jamur Collectotrichum acutatum Penyebab Penyakit Antraknosa pada Tanaman Cabai Merah Besar (Capsicum annuum L.) Unedo, Dion; Suprapta, Dewa Ngurah; Suniti, Ni Wayan
Agrotrop : Journal on Agriculture Science Vol 14 No 2 (2024)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2024.v14.i02.p11

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The Effectiveness of Rhizobacteria to Inhibit the Growth of the Fungus Colletotrichum acutatum that Causes Anthracnose Disease on chili pepper (Capsicum annum L.). Anthracnose in chili pepper caused by the fungus Colletotrichum acutatum is one of the main diseases that affect the production of chili pepper. The use of rhizobacteria as biological agents is considered effective to suppress the spread of anthracnose disease in chili pepper. This study tested the rhizobacteria isolated from the rhizosphere of Graminae and citrus plants to inhibit the growth of the fungus C. acutatum in vitro on PDA media and in vivo on chili peppers. The purpose of this study was to obtain rhizobacteria that could inhibit the growth of fungus C. acutatum, both on PDA media and on chili fruit. The test results showed that the rhizobacteria isolate Yj (isolated from the rhizosphere of citrus plants) could inhibit the growth of the fungus C. acutatum on PDA media by 87.16% compared to the control. In vivo test on chili fruit showed that rhizobacteria isolate Yj could inhibit the growth of C.acutatum fungal colonies by 64.17% when compared to control at 6 days after inoculation.
Uji Efektifitas Ekstrak Tanaman sebagai Nematisida Nabati terhadap Tingkat Fekunditas Nematoda Puru Akar (Meloidogyne Spp.) pada Tanaman Seledri (Apium graveolens L.) di Rumah Kaca Eaggeliony, Jenifer Christy; Singarsa, I Dewa Putu; Suniti, Ni Wayan
Agrotrop : Journal on Agriculture Science Vol 13 No 3 (2023)
Publisher : Fakultas Pertanian Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/AJoAS.2023.v13.i03.p11

Abstract

The Effective Test of Plant Extracts as A Nematicide for Fecundity of Root Knot Nematodes (Meloidogyne spp.) in Celery Plant (Apium graveolens L.) at Greenhouse. Root knot nematodes Meloidogyne spp. is among the harmful organism in plant cultivation, one of which is in the celery plant. Nematodes caused drop in/reduced plant productivity and plant quality, it can even lead to death and crop failure. Base on the adverse effects of nematodes attack, it must be anticipated. Biopesticides is one of the alternatives that can be used to suppress the growth of nematodes and more environmentally friendly. The purpose of this study is to know the effectiveness of the plant extracts that suppress the number of egg mass, the number of eggs per egg mass and the number of adult females in 1 g of roots. This study using a completely randomized design (CRD), with 5 types of treatments and each treatments received 5 repetitions. The treatments are by giving each plant extracts of 100 cc per pot. The result showed that the extract of marigold plants (Tagetes spp.) has the highest effectiveness, with the percentage of emphasis on the number of eggmass in 1 g roots is 99%, the emphasis on the number of eggs per eggmass in 1 g roots is 84,6% and the emphasis on the number of adult females in 1 g roots is 99,4%. While extract of sour-plant showed that has the lowest effectiveness, with the percentage of emphasis on the number of eggmass in 1 g roots is 96,68%, the emphasis on the number of eggs per eggmass in 1 g roots is 60,72% and the emphasis on the number of adult females in 1 g roots is 98,44%.