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Journal : E-Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology)

Deteksi Potyvirus pada Gulma Euphorbia heterophylla Bergejala Mosaik di Areal Pertanaman Melon di Kota Denpasar DEBBIE OKTAVIANI DEPARI; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.3, Juli 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Detection of Potyvirus in Mosaic Symptomatic Euphorbia heterophylla in Melon Planting Area in the city of Denpasar Melon (Cucumis melo L.) is a horticultural plant which is included in the Cucurbitaceae group and is highly favored by the community. The development of melon cultivation has faced some obstacles that cause the level of consumption of melon can not be fulfilled. Viruses in plants have a wide range of hosts including several weeds that can be alternative hosts and as a source of inoculums. Potyvirus group is the largest group of plant viruses and plays an important role in causing harm. This research was conducted to determine whether weeds can potentially as an alternative host of Potyvirus so that they can be used as reference materials for academics and farmers so that they take strategies to control weeds in melon plants. Variations in symptoms found in the field include mosaics, vein banding, vein clearing, and chlorosis. The method used in this study was the Polymerase Chain Reaction (PCR). The research activities include (1) sampling and (2) detection and identification of potyviruses in weeds. The results of this study found that Euphorbia heterophylla weeds could become alternative hosts for Potyvirus. The RT-PCR technique succeeded in amplifying target DNA fragments measuring 683 bp according to the CIFOR / CIREV universal primers used in samples from Denpasar.
Identifikasi Virus Penyebab Penyakit Mosaik pada Tanaman Melon (Cucumis melo L.) di Bali RIZWAN FEBIANTA BRAHMANA; TRISNA AGUNG PHABIOLA; I GEDE RAI MAYA TEMAJA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.9, No.1, Januari 2020
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Viruses that Causes Mosaic Diseases in Melons(Cucumis melo L.) in Bali The aims of this research was to identify of some virus that infects melons in Bali. The first step of this research was to take samples in Jembrana Regency, TabananRegency, Gianyar Regency, Denpasar City, and Badung Regency. The next step wasdoing virus detection at Laboratory of Plant Diseases, Faculty of Agriculture, UdayanaUniversity. Variations of symptoms found in the field were mosaic, yellow leaves,malformations, and burning spots on the leaves. The percentage of virus attacks inGianyar and Badung was the highest percentage (60.00%), Jembrana (57.14%),Denpasar (42.85%), and Tabanan (37.50%). To know type of virus that causes thisdisease, molecular identification was conducted. Extraction was the first step to gettotal RNAs from symptomatic leaves. Total RNAs was transcribed intoComplementary (c) DNAs using reverse transctiption-polymerase chain reaction (RTPCR)technique. Polymerase chain reaction (PCR) was conducted using cDNA and three primer pairs of CABYV-489-F/Pol-G-R, MABYV-262-F/Pol-G-R andCIFOR/CIREV. Primers would be use to amplified a part of coat protein. CABYV489-F/Pol-G-Rand MABYV-262-F/Pol-G-R is specific primer, and CIFOR/CIREV would be use to amplified gene of Potyvirus. Based on the results of molecular with PCR it was found that melons in Bali were positively infected with viruses from thePotyvirus group with a size of around 683 bp in samples from Gianyar and Denpasar.
Penentuan Fase Kritis Tanaman Kacang Panjang (Vigna sinensis L.) terhadap Infeksi Bean Common Mosaic Virus (BCMV) NI LUH OCTAVIANI; I MADE SUDANA; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.6, No.1, Januari 2017
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Determine of the Critical Phase of Long Bean Plants (Vigna sinensis L.) against Infection of Bean Common Mosaic Virus (BCMV)This study aims to search the critical phase of long bean plants (Vigna sinensis L.) against infection of Bean Common Mosaic Virus (BCMV). The method used is Indirect ELISA (Indirect enzyme-linked immunosorbent assay). This research activity includes variations of symptoms on the long bean plants samples, the incidence of the disease, calculation of yields, the determination of the critical times of the plant, Indirect ELISA serology testing, and data analysis. Design used was a randomized block design (RBD) with seven treatments and four replications.The results showed that the variation of symptoms long bean plants infected BCMV that mosaic, vein banding, malformation of the leaves, stunted and chlorosis. Higher incidence of disease inoculation treatment 2 weeks after planting (WAP) is 91%. Long bean plants are attacked by BCMV the highest yield losses amounted to 93.54% at 2 WAP, so the critical phase of the plant is on the treatment of long bean 2 WAP. Based on these studies need to be done at the beginning of growth and, if already infected plants in early growth, eradication needs to be done so as not to be a source of inoculum.
Tingkat Kerentanan Berbagai Umur Tanaman Melon (Cucumis melo L.) terhadap Infeksi Potyvirus I KADEK ARYARTHA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Susceptibility Levels of Various Ages of Melon Plants (Cucumis melo L.) Against Potyvirus Infection Domestic melon production has not been able to fulfill the community. Pest disruption is one of the main causes of declining melon production so that the community's demand for melons is not fulfilled. Potyvirus is a virus that is often found in melon plants in Bali, but information about the development of this disease is very limited. This study aims to determine the effect of time on the rate of Potyvirus infection in melon (Cucumis melo L.) plants. The steps taken in this study were preparing plants, inoculating Potyvirus in plants, observing disease progression and conducting research in the laboratory using the RT-PCR (Reverse Transcription Polymerase Chain Reaction) test. The results showed that the younger the melon plants infected by Potyvirus, the faster the rate of infection and the more severe the symptoms caused. The rate of infection in plants treated with inoculation at the age of 2, 3, 4, 5, 6, 7 weeks after planting and without inoculation treatment was 0.96 respectively; 0.60; 0.53; 0.41; 0.30; 0.18 and 0. Melon plants with inoculation treatment of 2, 3 and 4 weeks after planting were very susceptible to Potyvirus infection, which showed signs of severe mosaicism and severe malformations. Melon plants with inoculation treatment 5, 6 and 7 weeks after planting were more resistant to Potyvirus infection, namely showing moderate mosaic symptoms and mild mosaicism, while plants that were not given inoculation treatment showed no symptoms. Detection of Potyvirus using the RT-PCR method using primers (CI-FOR / CI-REV) successfully amplified Potyvirus fragments in the presence of base bands measuring ±683 bp in samples A, B, C, D, E, and F.
Identifikasi Penyebab Penyakit Bercak Merah pada Tanaman Stroberi (Fragaria sp.) di Bali dan Potensi Pengendaliannya dengan Jamur Antagonis Secara In Vitro I GUSTI AYU ARI SANTIKADEWI; GUSTI NGURAH ALIT SUSANTA WIRYA; TRISNA AGUNG PHABIOLA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Identification of Pathogenic Fungi causes Red Leaf Spot on Strawberry Plants (Fragaria sp.) in Bali and Control Potential with Antagonist Fungi Strawberry (Fragaria sp.) is a subtropical fruit plant that was first discovered in Chile, America and has high economic value. Strawberry plants growing in Bali show symptoms of pathogenic fungi attack. Symptoms include red spots on the leaf surface. The purpose of this study was to identify pathogenic fungi that cause disease in strawberry plants in the center of strawberry cultivation in Bali. The activities carried out in this study were (1) sampling, (2) isolation of pathogenic fungi from symptomatic strawberry plant parts, (3) pathogenicity test, (4) morphological identification of pathogenic fungi, and (5) potential inhibition test of antagonistic fungi. against pathogenic fungi. This research was conducted in Pancasari Village, Buleleng Regency and Candi Kuning and Kembang Mertha Villages, Tabanan Regency and continued at the Plant Disease Laboratory, Faculty of Agriculture, Udayana University, Denpasar. The results of this study succeeded in identifying Pestalotiopsis sp. as a pathogen causingred spot disease on strawberry leaves in Bali and the potential for biological control using antagonistic fungi in vitro showed that Gliocladium sp. and Trichoderma asperellum can suppress the growth of pathogenic fungi on strawberry plants by > 80%, Trichoderma viride up to > 90% and Trichoderma koningii and Trichoderma harzianum up to > 50%.
Metode Isolasi Jamur Patogen Serangga (Aschersonia placenta) Menggunakan Media Water Agar dan Potato Sucrose Agar NI MADE SAVITA RASJMAN; I PUTU SUDIARTA; TRISNA AGUNG PHABIOLA; GUSTI NGURAH ALIT SUSANTA WIRYA; I PUTU WIRYA SUPUTRA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.2, April 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Isolation Method of Insect Pathogenic Fungi (Aschersonia placenta) using Water Agar Media and Potato Sucrose Agar Bali Province is one of the provinces that is famous for its superior agricultural commodities. One of the leading agricultural commodities is citrus. The production of citrus fruits in Bali Province in 2020 is 4,903,341 quintals based on data from the Central Statistics Agency for Bali Province 2021. However, in some citrus-producing areas, citrus fruit production has decreased compared to the previous year. The decline in citrus fruit production is caused by several factors, one of which is the attack of whitefly pests. Whitefly pests can be controlled by utilizing the insect pathogenic fungus Aschersonia placenta. This study aims to determine the isolation method of insect pathogenic fungi Aschersonia placenta using water agar media and potato sucrose agar. The study began with sampling, isolated the fungus and identifying the fungus by morphological characteristics. The results of this study showed that isolation method of A. placenta using water agar media and potato sucrose agar effective for multiply the fungus.
Uji Antagonistik Bakteri Penghasil Indole Acetic Acid Terhadap Jamur Helminthosporium maydis Secara In Vitro HELENA TAMPUBOLON; KHAMDAN KHALIMI; TRISNA AGUNG PHABIOLA
Jurnal Agroekoteknologi Tropika (Journal of Tropical Agroecotechnology) Vol.11, No.4, Oktober 2022
Publisher : Program Studi Agroekoteknologi, Fakultas Pertanian, Universitas Udayana

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Antagonistic Test of Indole Acetic Acid Producing Bacteria Against Helminthosporium maydis In Vitro Helminthosporium maydis is the cause of important diseases in maize, including leaf blight. Leaf blight is the most dominant disease because it causes large losses. The use of biological agents is one way to treat leaf blight that is environmentally friendly. One of these biological agents is IAA-producing bacteria. This research was conducted in vitro. The results showed that there were several IAA-producing bacteria that were able to inhibit the growth of H. maydis in vitro with the highest inhibition in the treatment of B. thuringiensis GR12 isolates of 96.44% when compared to controls. IAA-producing bacteria were able to inhibit the growth of H. maydis biomass with the percentage of inhibition ranging from 73.97% - 80.82%. The treatment of IAA-producing bacterial filtrate at a concentration of 20%-90% was able to inhibit the growth of H. maydis colonies in vitro. The results of this study indicate that IAA compounds produced by bacteria are antifungal.