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All Journal HAYATI Journal of Biosciences METAMORFOSA Journal of Biological Sciences Jurnal Ilmu Dasar BERKALA SAINSTEK The Journal of Pure and Applied Chemistry Research Global Medical and Health Communication Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI AL-HAYAT: Journal of Biology and Applied Biology Life Science and Biotechnology Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember Journal of Coffee and Sustainability
Sattya Arimurti
Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Jember, Jln. Kalimantan 37, Jember 68121
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Arimurti, Sattya; Utarti, esti; sutoyo, sutoyo; siswanto, siswanto; Ayuningtyas, Tantri Raras
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Abstract

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (λ) reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.
Does the chick of domestik chicken (Gallus gallus domesticus) in early development would able to witstand the injection of crude LPS? lelono, Asmoro; Laensugi, Agatha Dei Gratia; Arimurti, Sattya
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53503

Abstract

Preventing bacterial infections in poultry farms is a key aspect of effective disease management strategies. Enhancing innate immunity serves as a vital preventive measure in chicken farming. Lipopolysaccharide (LPS), a fundamental component of the bacterial cell wall, has been successfully utilized to boost the innate immune response in susceptible poultry hosts. This study focused on examining the impact of administering crude LPS from Salmonella typhimurium to a domestic chicken (Gallus gallus domesticus) strain during its early developmental stages up to the pre-maturation phase. Immune responses in the chicks were evaluated by observing changes in body temperature and leukocyte differentiation. The chickens were divided into two groups: control (injected with 0.85% NaCl) and treatment (injected with the LPS). The injections were applied in three different ages 7 days, 24 days, and 41 days. The body temperature and leukocyte differential data were collected following the LPS injection. Results showed a significant difference in body temperature and heterophil at the ages of 24 and 41, indicating an immune response characterized by inflammation and elevated heterophil levels. There was a significant increase in basal temperature during early development in line with chicks' development to maintain their homeostatic system. This study concludes that the chicks demonstrated resilience to the presence of LPS by exhibiting heightened responses. The increasing of heterophils concentration provided strong evidence of their immune capability to overcome bacterial infections
A Growth Pattern, and Caffeine Degradation of Acinetobacter gerneri KAFS 47 on Glucose Media with Addition of Caffeine Arimurti, Sattya; Wicaksono, Bagus Aji; Siswanto, Siswanto
Journal of Coffee and Sustainability Vol. 1 No. 2 (2024)
Publisher : Directorate of Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jcs.2024.01.02.04

Abstract

Caffeine-degrading bacteria are organisms capable of utilizing caffeine as a carbon and nitrogen source. Acinetobacter gerneri KAFS 47 is known to possess the ability to degrade caffeine. The type of carbon source present in the growth medium influences the growth pattern and the caffeine degradation capabilities of caffeine-degrading bacteria. The objective of this study was to ascertain the growth pattern of A. gerneri KAFS 47 and to analyze the pattern of caffeine degradation by A. gerneriKAFS 47 in growth media following the addition of caffeine during the logarithmic phase.  The research methodology encompasses the following steps: 1.) Constructing a growth curve for A. gerneri KAFS 47 using M9 supplemented with 2.5 g/L glucose, M9 supplemented with 2.5 g/L caffeine, M9 supplemented with 2.5 g/L glucose with an additional 2.5 g/L caffeine introduced midway through the logarithmic phase (12 hours), and M9 supplemented with 2.5 g/L glucose with an added 5 g/L caffeine at the midpoint of the logarithmic phase (12 hours); and  2.) developing a caffeine degradation curve for A. gerneri KAFS 47. The addition of caffeine during the logarithmic phase at concentrations of 2.5 g/L and 5 g/L enhanced the logarithmic growth phase of  A. gerneriKAFS 47 in media with a glucose carbon source. Notably, the degradation of caffeine by A. gerneri KAFS 47 was observed to be slower in media containing glucose with added caffeine (2.5 g/L and 5 g/L) compared to media where caffeine served as the sole carbon source.
Co-Authors AA Sudharmawan, AA Adi Sucipto, Adi Agung Nugroho Puspito Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Arif Mohammad Siddiq Arif Setiawan Ayuningtyas, Tantri Raras Azhari, Fitri Dina Amalia Syahidah DINA FITRIYAH Dwi Setyati Esti Utarti Evi Umayah Ulfa Farrennina, Tasya Preira Fuad Bahrul Ulum Hari Sulistyowati Hasanah, Lailiyah Maulidatul Ika Oktavianawati Izzay Afkarina Kahar Muzakhar Kartika Senjarini Kholiq, Nur Laensugi, Agatha Dei Gratia Lailiyah Maulidatul Hasanah Lelono, Asmoro Lina Nurita Luthfiah Luthfiah Mukhamad Su'udi Puspito, Agung Nugroho Putri, Amelia Fahreza S Siswanto Su'udi, Mukhamad Suharjono Suharjono Suksma, Nadhea Ayu Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Tabah Wibisono, Hariyo Wicaksono, Bagus Aji Wimbaningrum, Retno Winarsa, Rudju