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The HEMOLYSIS ACTIVITY OF BACTERIA ISOLATS FROM PELANGI FOREST OF IJEN GEOPARK Arimurti, Sattya; Utarti, esti; sutoyo, sutoyo; siswanto, siswanto; Ayuningtyas, Tantri Raras
Life Science and Biotechnology Vol. 1 No. 2 (2023): November 2023
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v1i2.44097

Ijen geopark is one of Indonesia's geoparks, which is located in East Java. A total of 153 bacteria have been isolated from Pelangi Forest, which were then given the isolate code IHP. These bacterial isolates can be utilized in industries, including organic matter decomposer agents, plant biocontrol agents, and probiotics. To ensure these bacteria are safe to use in various fields, they must be non-disease-causing (non-pathogenic). Safety evaluations based on the hemolysis reactions offer simple tests to ease the analysis of potential pathogenic bacteria. The study aimed to evaluate the safety of bacterial isolates from Pelangi Forest for their hemolysis reactions. The hemolysis test was conducted using blood agar media, from which isolates with a negative (Î») reaction. Based on the results of hemolysis tests, 30 out of 153 bacterial isolates (19.60%) were found to be negative reactions. These bacteria are safe to proceed with for potential analysis.

Does the chick of domestik chicken (Gallus gallus domesticus) in early development would able to witstand the injection of crude LPS? lelono, Asmoro; Laensugi, Agatha Dei Gratia; Arimurti, Sattya
Life Science and Biotechnology Vol. 2 No. 2 (2024): November 2024
Publisher : Department of Biology, Faculty Mahematics and Natural Sciences, University of Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/lsb.v2i2.53503

Preventing bacterial infections in poultry farms is a key aspect of effective disease management strategies. Enhancing innate immunity serves as a vital preventive measure in chicken farming. Lipopolysaccharide (LPS), a fundamental component of the bacterial cell wall, has been successfully utilized to boost the innate immune response in susceptible poultry hosts. This study focused on examining the impact of administering crude LPS from Salmonella typhimurium to a domestic chicken (Gallus gallus domesticus) strain during its early developmental stages up to the pre-maturation phase. Immune responses in the chicks were evaluated by observing changes in body temperature and leukocyte differentiation. The chickens were divided into two groups: control (injected with 0.85% NaCl) and treatment (injected with the LPS). The injections were applied in three different ages 7 days, 24 days, and 41 days. The body temperature and leukocyte differential data were collected following the LPS injection. Results showed a significant difference in body temperature and heterophil at the ages of 24 and 41, indicating an immune response characterized by inflammation and elevated heterophil levels. There was a significant increase in basal temperature during early development in line with chicks' development to maintain their homeostatic system. This study concludes that the chicks demonstrated resilience to the presence of LPS by exhibiting heightened responses. The increasing of heterophils concentration provided strong evidence of their immune capability to overcome bacterial infections

A Growth Pattern, and Caffeine Degradation of Acinetobacter gerneri KAFS 47 on Glucose Media with Addition of Caffeine Arimurti, Sattya; Wicaksono, Bagus Aji; Siswanto, Siswanto
Journal of Coffee and Sustainability Vol. 1 No. 2 (2024)
Publisher : Directorate of Research and Community Services

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jcs.2024.01.02.04

Caffeine-degrading bacteria are organisms capable of utilizing caffeine as a carbon and nitrogen source. Acinetobacter gerneri KAFS 47 is known to possess the ability to degrade caffeine. The type of carbon source present in the growth medium influences the growth pattern and the caffeine degradation capabilities of caffeine-degrading bacteria. The objective of this study was to ascertain the growth pattern of A. gerneri KAFS 47 and to analyze the pattern of caffeine degradation by A. gerneriKAFS 47 in growth media following the addition of caffeine during the logarithmic phase. The research methodology encompasses the following steps: 1.) Constructing a growth curve for A. gerneri KAFS 47 using M9 supplemented with 2.5 g/L glucose, M9 supplemented with 2.5 g/L caffeine, M9 supplemented with 2.5 g/L glucose with an additional 2.5 g/L caffeine introduced midway through the logarithmic phase (12 hours), and M9 supplemented with 2.5 g/L glucose with an added 5 g/L caffeine at the midpoint of the logarithmic phase (12 hours); and 2.) developing a caffeine degradation curve for A. gerneri KAFS 47. The addition of caffeine during the logarithmic phase at concentrations of 2.5 g/L and 5 g/L enhanced the logarithmic growth phase of A. gerneriKAFS 47 in media with a glucose carbon source. Notably, the degradation of caffeine by A. gerneri KAFS 47 was observed to be slower in media containing glucose with added caffeine (2.5 g/L and 5 g/L) compared to media where caffeine served as the sole carbon source.

Kapang amilolitik asal Biosite Hutan Pelangi, Ijen Geopark, Indonesia Sutoyo Sutoyo; Safira Isti’nafil Islam; Esti Utarti; Sattya Arimurti; Siswanto Siswanto
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p10

Vegetasi Hutan Pelangi, Ijen Geopark sudah terkonservasi sejak lama, sehingga kemungkinan di dalamnya terdapat diversitas mikrobiota yang potensial sebagai agen hayati pendegradasi berbagai substrat organik. Keberadaan dan pertumbuhan mikroba dalam lingkungan Hutan Pelangi berperan penting dalam membentuk proses antara lain siklus karbon, sehingga keberadaan mikroorganisme tersebut menarik untuk dikaji secara mikrobiologi. Penelitian ini bertujuan untuk mendapatkan isolat yang mampu mendegradasi substrat amilum secara semikuantitatif dan identifikasi secara morfologi sampai tingkat genus terhadap isolat terpilih. Penelitian diawali dengan isolasi, skrining kapang amilolitik serta identifikasinya secara fenotip terhadap isolat yang potensial aktivitas degradasinya tinggi. Hasil penelitian diperoleh 34 isolat kapang yang menunjukkan keragaman berbeda secara morfologi koloninya dan aktivitasnya dalam menghidrolisis amilum. Sejumlah 22 isolat (64,7%) menunjukkan aktivitas amilolitik dan 12 isolat (35,29%) merupakan kapang non-amilolitik. Hasil ANOVA rata-rata indeks aktivitas amilolitik isolat kapang dan uji lanjut Duncan menunjukkan bahwa isolat kapang IHP30, IHP10, dan IHP9 merupakan tiga isolat kapang amilolitik dengan indeks aktivitas amilolitik tertinggi. Indeks aktivitas masing-masing yaitu isolat kapang IHP30 sebesar 1,65 ± 0,14; IHP10 sebesar 1.47 ± 0,31; dan IHP9 sebesar 1.17 ± 0,06. Berdasarkan karakteristiknya, masing-masing teridentifikasi sebagai Penicillium sp. IHP30, Penicillium sp. IHP10, dan Aspergillus sp. IHP9

Kapang amilolitik asal Biosite Hutan Pelangi, Ijen Geopark, Indonesia Sutoyo Sutoyo; Safira Isti’nafil Islam; Esti Utarti; Sattya Arimurti; Siswanto Siswanto
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p10

Vegetasi Hutan Pelangi, Ijen Geopark sudah terkonservasi sejak lama, sehingga kemungkinan di dalamnya terdapat diversitas mikrobiota yang potensial sebagai agen hayati pendegradasi berbagai substrat organik. Keberadaan dan pertumbuhan mikroba dalam lingkungan Hutan Pelangi berperan penting dalam membentuk proses antara lain siklus karbon, sehingga keberadaan mikroorganisme tersebut menarik untuk dikaji secara mikrobiologi. Penelitian ini bertujuan untuk mendapatkan isolat yang mampu mendegradasi substrat amilum secara semikuantitatif dan identifikasi secara morfologi sampai tingkat genus terhadap isolat terpilih. Penelitian diawali dengan isolasi, skrining kapang amilolitik serta identifikasinya secara fenotip terhadap isolat yang potensial aktivitas degradasinya tinggi. Hasil penelitian diperoleh 34 isolat kapang yang menunjukkan keragaman berbeda secara morfologi koloninya dan aktivitasnya dalam menghidrolisis amilum. Sejumlah 22 isolat (64,7%) menunjukkan aktivitas amilolitik dan 12 isolat (35,29%) merupakan kapang non-amilolitik. Hasil ANOVA rata-rata indeks aktivitas amilolitik isolat kapang dan uji lanjut Duncan menunjukkan bahwa isolat kapang IHP30, IHP10, dan IHP9 merupakan tiga isolat kapang amilolitik dengan indeks aktivitas amilolitik tertinggi. Indeks aktivitas masing-masing yaitu isolat kapang IHP30 sebesar 1,65 ± 0,14; IHP10 sebesar 1.47 ± 0,31; dan IHP9 sebesar 1.17 ± 0,06. Berdasarkan karakteristiknya, masing-masing teridentifikasi sebagai Penicillium sp. IHP30, Penicillium sp. IHP10, dan Aspergillus sp. IHP9

Thrombolytic Activity and Protein Characterization of Extract from White Oyster Mushroom (Pleurotus ostreatus) Alishlah, Tanfidz; Umayah Ulfa, Evi; Arimurti, Sattya; Eka Putri, Khilwiyah
Journal of Food and Pharmaceutical Sciences Vol 13, No 4 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25015

Venous thromboembolism (VTE) is a condition resulting from the presence of thrombi within blood vessels. Globally, these illnesses are the leading causes of death. In 2022, the WHO estimates that 19.8 million fatalities or 32% of worldwide deaths were a result of cardiovascular disease, which is initiated by thromboembolism. The use of thrombolytic medicines is one treatment option for thromboembolic illness. The exploration revealed that numerous mushrooms contain protease enzymes with a thrombolytic effect. A common edible mushroom in Indonesia is the white oyster mushroom (Pleurotus ostreatus). By assessing thrombolytic activity using the Blood Clot Lysis Assay method and characterizing proteins using SDS-PAGE electrophoresis, this study aims to determine the thrombolytic activity of the extract and crude protein from the white oyster mushroom. The findings demonstrated the thrombolytic activity of the extract and crude protein of oyster mushroom at 29.89% and 37.89%, respectively. The extracts with volumes of 500 µl and the crude protein with a concentration of 0.05 g/mL exhibited the highest thrombolytic activity. The thrombolytic activity results of the extract (500 µl) and crude proteins (0.02 g/mL and 0.05 g/mL) of the white oyster mushroom were significantly higher than the positive control (p<0.05). According to protein characterization, the white oyster mushroom extract and crude protein had protein molecular weights of 45.50 kDa, 32.51 kDa, 21.09 kDa, and 15.1 kDa, respectively. White oyster mushroom extracts and crude protein have the potential to be developed as thrombolytic agents, serving as a safe, natural, and cost-effective alternative for thromboembolism disorders. While the results offer an important foundation, further studies are necessary to confirm and extend these findings.

Thrombolytic Activity and Protein Characterization of Extract from White Oyster Mushroom (Pleurotus ostreatus) Alishlah, Tanfidz; Umayah Ulfa, Evi; Arimurti, Sattya; Eka Putri, Khilwiyah
Journal of Food and Pharmaceutical Sciences Vol 13, No 4 (2025): J.Food.Pharm.Sci
Publisher : Integrated Research and Testing Laboratory (LPPT) Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jfps.25015

Venous thromboembolism (VTE) is a condition resulting from the presence of thrombi within blood vessels. Globally, these illnesses are the leading causes of death. In 2022, the WHO estimates that 19.8 million fatalities or 32% of worldwide deaths were a result of cardiovascular disease, which is initiated by thromboembolism. The use of thrombolytic medicines is one treatment option for thromboembolic illness. The exploration revealed that numerous mushrooms contain protease enzymes with a thrombolytic effect. A common edible mushroom in Indonesia is the white oyster mushroom (Pleurotus ostreatus). By assessing thrombolytic activity using the Blood Clot Lysis Assay method and characterizing proteins using SDS-PAGE electrophoresis, this study aims to determine the thrombolytic activity of the extract and crude protein from the white oyster mushroom. The findings demonstrated the thrombolytic activity of the extract and crude protein of oyster mushroom at 29.89% and 37.89%, respectively. The extracts with volumes of 500 µl and the crude protein with a concentration of 0.05 g/mL exhibited the highest thrombolytic activity. The thrombolytic activity results of the extract (500 µl) and crude proteins (0.02 g/mL and 0.05 g/mL) of the white oyster mushroom were significantly higher than the positive control (p<0.05). According to protein characterization, the white oyster mushroom extract and crude protein had protein molecular weights of 45.50 kDa, 32.51 kDa, 21.09 kDa, and 15.1 kDa, respectively. White oyster mushroom extracts and crude protein have the potential to be developed as thrombolytic agents, serving as a safe, natural, and cost-effective alternative for thromboembolism disorders. While the results offer an important foundation, further studies are necessary to confirm and extend these findings.

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