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All Journal HAYATI Journal of Biosciences Journal of Food and Pharmaceutical Science Jurnal Ilmu Dasar BERKALA SAINSTEK Pelita Perkebunan The Journal of Pure and Applied Chemistry Research Global Medical and Health Communication Jurnal Bioteknologi & Biosains Indonesia (JBBI) BIOEDUKASI AL-HAYAT: Journal of Biology and Applied Biology Journal of Food and Pharmaceutical Sciences Life Science and Biotechnology Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember Journal of Coffee and Sustainability Metamorfosa: Journal of Biological Science Open Access DRIVERset
Sattya Arimurti
Department Of Biology, Faculty Of Mathematics And Natural Science, University Of Jember, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Control & Systems Engineering Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Characterization and Identification of Caffeine-Degrading Bacteria KAJ 36 Sattya Arimurti; Reza Billa Afifuddin; Siswanto Siswanto; Kahar Muzakhar
Pelita Perkebunan (a Coffee and Cocoa Research Journal) Vol 36 No 2 (2020)
Publisher : Indonesian Coffee and Cocoa Research Institute

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/iccri.jur.pelitaperkebunan.v36i2.439

Abstract

Caffeine could be utilized by caffeine-degrading bacteria as a source of carbon and nitrogen. These bacteria have the potential as an agent of decaffeinating coffee. The objective of this research was to characterize and identify the caffeinedegrading bacteria KAJ 36 that was isolated from the pulp waste of Coffea arabica. The isolate-characterization was performed based on its growth and caffeine degradation activity.in a medium M9 added with 10 g/L caffeine for 7 days. The isolate identification was conducted using 16S rRNA sequence analysis and biochemical analysis. The result indicated that caffein-degrading bacteria were able to grow and degrade the caffein up to 86% activity on M9 medium added 10 g/L caffeine for 7 days incubation. Based on 16S rRNA, the isolated KAJ 36 had sequences of 99% homology with Pseudomonas monteilii. Biochemical analysis showed that this isolation on the specific medium was a gram-negative, oxidative, positive enzymatic activity (oxidase, catalase, urease) and reduce nitrate. Our results showed that the isolated KAJ 36 was capable of producing fluorescent pigment. This bacterium was unable to produce indole or utilize sucrose and lactose. Based on the cultural characteristics, we found that the isolated KAJ 36 could be grown in a medium with pH 5, 7, and 9, and in the environment with up to 37oC. This evidence suggested that P. monteilii KAJ 36 can be used as a potential degradation agent of caffeine and recommend for future research in the bacterial based on the degradation of caffeine-contained coffee.
Growth Pattern and Degradation Activity of Caffeine-degrading Bacteria Consortium Suksma, Nadhea Ayu; Utarti, Esti; Arimurti, Sattya
Jurnal ILMU DASAR Vol 25 No 1 (2024)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v25i1.32609

Abstract

Caffeine-degrading bacteria can be used as agents to degrade caffeine, thereby reducing the concentration of caffeine in organic waste. The decomposition process is carried out by a single bacterium or a consortium of bacteria. Caffeine-degrading bacteria from Sempol, Bondowoso, namely Acinetobacter gerneri KAFS 47, Paracoccus denitrificans KAFS 16 and Pseudomonas plecoglossicida KAFS 34, could be used as a bacterial consortium to promote caffeine degradation. The aim of this study was to analyze associations between caffeine-degrading bacteria isolates, bacterial resistance to antibiotics, growth patterns, and caffeine degradation of a consortium of caffeine-degrading bacteria, and the correlation of bacterial growth with caffeine degradation. The research method used is an analysis of the association between isolates, the development of bacterial consortium growth patterns, and their analysis based on antibiotic resistance, patterning of caffeine degradation, and correlation test (Pearson) of bacterial growth with caffeine degradation. The result of the association test between bacteria showed that the three bacteria had the potential to be used as a consortium of caffeine-degrading bacteria. A. Gerneri, P. denitrificans, and P. plecoglossicida were resistant to the antibiotic cefixime (100 ppm), erythromycin (50 ppm), lincomycin (50 ppm), metronidazole (50 ppm), and sanprima (50 ppm). The growth of the bacterial consortium (54.779 CFU/mL) was higher than that of P. plecoglossicida (49.277 CFU/mL) and lower than that of A. gerneri (93.481 CFU/mL) and P. denitrificans (84.940 CFU/mL) in incubation time of 4 days. However, the consortium of bacteria and P. plecoglossicida were able to degrade caffeine 24 hours faster (3 days) than the other two single isolates (4 days) to degrade 2.5 g/L caffeine in media to 0%. Bacterial growth due to caffeine degradation has a perfect correlation value (>0. 950) and is negative.
SKRINING BAKTERI SELULOLITIK ASAL VERMICOMPOSTING TANDAN KOSONG KELAPA SAWIT Azizah, Siti Nur; Muzakhar, Kahar; Arimurti, Sattya
BERKALA SAINSTEK Vol 2 No 1 (2014)
Publisher : Universitas Jember

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Abstract

Biomassa Tandan Kosong Kelapa Sawit (TKKS) dihasilkan dalam jumlah melimpah selama pemanenan, sehingga harus didekomposisi dalam waktu singkat. Melalui vermicomposting, TKKS dikonversi menjadi kompos yang berlangsung selama 2-3 bulan, sehingga untuk mempercepat proses dekomposisi, penelitian ini perlu dilakukan. Lima puluh satu isolat bakteri selulolitik berhasil diisolasi dari vermicomposting limbah TKKS. Hasil uji pada media CMC (Carboxymethyl Cellulose) plate, empat isolat memiliki aktivitas selulolitik tertinggi, yaitu isolat 20, 40a, 40b dan 49 dengan indeks aktivitas sebesar 11,90; 10,97; 11,29, dan 11,24. Selama hidrolisis menggunakan substrat CMC dan TKKS, isolat 20 mampu memproduksi gula reduksi tertinggi yaitu sebesar 12,27 μg/mL dan 49,31 μg/mL, sedangkan isolat 40a, 40b, dan 49 sebesar 3,48 μg/mL, 6,28 μg/mL dan 3,10 μg/mL di substrat CMC dan sebesar 24,83 μg/mL, 11,21 μg/mL dan 8,25 μg/mL di substrat TKKS. Keempat isolat bakteri termasuk bakteri Gram negatif dengan bentuk sel batang. Kata Kunci: bakteri selulolitik, gula reduksi, vermicomposting TKKS.
Antibacterial Activity of Liverworts of Dumortiera hirsute (Sw.) Nees Ethyl Acetate Extract Against Pathogenic Bacteria Luthfiah, Luthfiah; Setyati, Dwi; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.22645

Abstract

Dumortiera hirsuta is one of the liverworts that can be used as a medicinal to prevent infection by pathogenic bacteria. The content of secondary metabolites of D. hirsuta has potential as antibacterial properties includes flavonoids, alkaloids and steroids. This research is to analyze the antibacterial activity of moss D. hirsuta against pathogenic bacteria that will be beneficial to humans. Liverworts of D. hirsuta were extracted using ethyl acetate solvent and tested against three types of pathogenic bacteria using the agar well-diffusion method. The results of this study indicated that the ethyl acetate extract of D. hirsuta at 100% concentration could inhibit the growth of Escherichia coli, Staphylococcus aureus, and Salmonella typhi bacteria. The range of antibacterial activity categories of the ethyl acetate extract of D. hirsuta to E. coli, S. aureus, and S. typhi between weak to moderate.
Growth of Lactobacillus casei FNCC0900 in Media Based Umbi Porang Plant (Amorphophallus muelleri BI.) Azhari, Fitri; Winarsa, Rudju; Siswanto, Siswanto; Muzakhar, Kahar; Utarti, Esti; Sutoyo, Sutoyo; Arimurti, Sattya
BERKALA SAINSTEK Vol 9 No 2 (2021)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v9i2.19034

Abstract

Porang tuber (Amorphophallus muellerii BI.) Is a type of tuber that has a high enough glucomannan content of 67%. Glucomannan is very difficult to digest by humans directly so it takes the role of probiotics. L. casei bacteria FNCC0900 as a probiotic agent capable of utilizing glucomannan as a carbon source for growth. The purpose of this study was to determine the growth pattern and changes in environmental factors, namely the pH value of the probiotic bacteria L. casei FNCC0900 growth medium. The parameters in this study consisted of the highest cell density, generation time and pH value changes in Glucose Yeast Peptone Liquid Media, Porang Boiled Water Media and Porang Flour Liquid Media using the drop plate method which had 4 repeated calculations. Porang Boiled Water Liquid Media has a faster log phase period with a higher cell density than Porang Flour Liquid Media, but the shortest generation time is found in Porang Flour Liquid Media with the highest number of generations. L. casei FNCC0900 bacteria are more able to reduce the pH of Glucose Yeast Peptone Liquid Media compared to porang tuber-based media, so in this case L. casei FNCC0900 can be stated to be able to grow on porang tuber-based media with growth patterns, generation time, cell density and pH value. which varies.
AKTIVITAS PROTEOLITIK DAN FIBRINOLITIK ISOLAT BAKTERI DARI PERAIRAN PANTAI PAPUMA KABUPATEN JEMBER Setiawan, Arif; Arimurti, Sattya; Senjarini, Kartika; Sutoyo, Sutoyo
BERKALA SAINSTEK Vol 4 No 1 (2016)
Publisher : Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bst.v4i1.4457

Abstract

Berbagai jenis bakteri dari perairan pantai dapat dieksplorasi sebagai sumber penghasil senyawa penting. Sejauh ini, bakteri dari Perairan Pantai Papuma Kabupaten Jember yang memiliki aktivitas proteolitik dan fibrinolitik belum dilaporkan. Tujuan penelitian ini adalah menguji aktivitas proteolitik dan fibrinolitik pada sejumlah isolat bakteri. Metode penelitian meliputi uji aktivitas proteolitik menggunakan Media Skim Milk Agar (SMA) terhadap 23 isolat bakteri dan uji aktivitas fibrinolitik menggunakan Media Fibrin pada isolat yang diketahui memiliki aktivitas proteolitik. Metode uji aktivitas dilakukan secara semikuantitatif. Sebanyak 11 isolat bakteri memiliki aktivitas proteolitik. Isolat bakteri WU 021012* memiliki indeks proteolitik tertinggi yaitu 4,3. Isolat bakteri yang fibrinolitik diperoleh sebanyak 3 isolat. Isolat bakteri WU 021055* memiliki indeks tertinggi sebesar 11.
PENDAMPINGAN PRAKTIKUM PENGENALAN MIKROSKOP SERTA BENTUK DAN STRUKTUR SEL PADA MAHASISWA PRODI ILMU KEPERAWATAN STIKES DR. SOEBANDI JEMBER SECARA ONLINE Fuad bahrul Ulum; Sutoyo; Sattya Arimurti
Jurnal Hasil Pengabdian kepada Masyarakat Universitas Jember Vol 1 No 1 (2022): Jurnal Hasil Pengabdian Kepada Masyarakat Universitas Jember
Publisher : LP2M Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jpmunej.v1i1.55

Abstract

Pembelajaran dengan pendekatan pengembangan ketrampilan merupakan strategi yang sering dipakai dalam pembentukan sikap ilmiah. Prakatikum merupakan metode pembelajaran yang diselenggarakan terhadapa mahasiswa STIKES dr. Soebandi Jember dalam pengenalan dan penggunaan Mikroskop, serta pendalaman materi pembelajaran bentuk dan struktur sel. Pelaksanaan praktikum selama pandemi Covid-19 di Jurusan biologi Universitas Jember dilakukan secara online. Tujuan penulisan artikel ini adalah untuk mengetahui efektifitas pembelajaran online terhadap capaian pembelajaran yang direkam dari kegiatan praktikum. Penelitian dilakukan dengan analisis data capaian pembelajaran dan menguji signifikansi skor rerata nilai laporan praktikum dan membandingkannya dengan tes akhir. Hasil penelitian diketahui bahwa capaian pembelajaran terendah adalah tes akhir yang dipengaruhi oleh beban materi pembelajaran yang diujikan. Pembelajaran berbasis aktivitas dan dilengkapi dengan kecukupan pengetahuan yang dimiliki oleh mahasiswa memberikan hasil optimal dalam penilaian capaian pembelajaran
Identifikasi WIN1 (Wax Inducer1) Pada Tanaman Ubi Kayu (Manihot esculenta Crantz.) Su'udi, Mukhamad; Hasanah, Lailiyah Maulidatul; Puspito, Agung Nugroho; Arimurti, Sattya
Al-Hayat: Journal of Biology and Applied Biology Vol. 4 No. 2 (2021)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v4i2.8558

Abstract

Tanaman pangan di Indonesia sangat beragam termasuk diantaranya padi, jagung dan ubi kayu. Namun lahan pertanian di Indonesia semakin sempit seiring dengan laju pertumbuhan penduduk yang semakin tinggi. Lahan kering (sub optimal) di Indonesia masih banyak yang belum dimanfaatkan, yang sebenarnya bisa dioptimalkan sebagai lahan pertanian. Tanaman pangan yang memiliki tingkat ketahanan lebih tinggi terhadap lahan kering adalah tanaman ubi kayu, sehingga berpotensi untuk ditanam pada lahan kering. Tahap pertama pemuliaan tanaman ubi kayu adalah mengetahui karakterisasi gen pada tanaman tersebut salah satunya yaitu WIN1. Metode pertama yang dilakukan adalah isolasi DNA daun ubi kayu varietas Adira 1 dan Malang 6, kemudian amplifikasi dengan PCR, dan analisis sekuensing. Hasil menunjukkan tanaman ubi kayu Adira 1 memiliki asam amino yang sama dengan kultivar am560-2 yang terdapat pada GenBank, sedangkan pada varietas Malang 6 terdapat satu asam amino yang berbeda. Hal tersebut dapat dipengaruhi oleh adanya mutasi gen pada varietas ubi kayu serta perbedaan kandungan HCN pada ubi kayu pangan varietas Adira 1, dan ubi kayu industri varietas Malang 6.
Assessing The Javan Leopard Presence and Prey Abundance: Insights from Camera Trap Surveys in Meru Betiri National Park East Java Indonesia Kholiq, Nur; Sulistyowati, Hari; Tabah Wibisono, Hariyo; Asmoro Lelono; Retno Wimbaningrum; Sattya Arimurti; Arif Mohammad Siddiq; Adi Sucipto
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 2 (2025)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i2.53715

Abstract

The use of incomplete or imbalanced data in ecological modelling and conservation planning can lead to inaccurate predictions due to limitations in detection methods. Therefore, obtaining comprehensive and precise empirical data on species presence and availability, particularly prey species is essential. This study aims to: (1) identify the presence of Javan leopards and their potential prey species, and (2) estimate the relative abundance indices (RAI) of each prey species. Using camera trap data from the Meru Betiri National Park (MBNP) survey conducted in 2024, we recorded 24 species, including the Javan leopard, and identified seventh potential prey species. We estimated relative abundance of each potential prey species was determined by dividing the number of individuals recorded in independent photos or videos by the total number of trap days and multiplied by 100. The result of RAI analysis indicated that the most abundant prey species in the study area was the long-tailed macaque, with an RAI value of 44.16 individuals per 100 trap-days, banteng was the least frequently detected species, appearing in only three independent images, with an RAI value of 0.23. The presence of Javan leopards, a charismatic flagship species, highlights the ecological significance of this area and provides valuable data for biodiversity management, serving as a foundation for future research and conservation efforts.
Production and Characterization od Xylanase from Actinomyces ATG 70 Using CornCob Xylan Substrate Farrennina, Tasya Preira; Putri, Amelia Fahreza; Arimurti, Sattya; Winarsa, Rudju; Utarti, Esti
Jurnal ILMU DASAR Vol. 26 No. 2 (2025)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/jid.v26i2.53702

Abstract

Xilan is a polysaccharide that can be used as a substrate for producing xylanase. Xylan can be obtained from processing xylan-rich agricultural waste such as corn cobs, which contain 12.4% xylan. Xilan can be hydrolyzed into xylose and xylooligosaccharide (XOS) using the enzyme Xilanase. Actinomycetes have dominant xylanolytic species, Actinomyces ATG 70 has semi-qualitative xylanolytic ability with an enzyme activity index of 3.21±0.55 in xylan media. This study was conducted by extracting xylan from corn cobs, rejuvenating Actinomyces ATG 70, preparing the inoculum, creating a standard curve, producing xylanase with pH optimization of the medium, testing xylanase activity, characterizing the pH and temperature of crude xylanase, and identifying the morphology and biochemical characteristics of Actinomyces ATG 70. Optimization of the pH of the xylanase production medium was achieved at pH 8, with xylanase activity of 10.07±0.13 U/mL. The crude xylanase was characterized for pH and temperature effects, and the optimal pH for crude xylanase was found to be pH 6 with xylanase activity of 7.64±0.66 U/mL, and the optimal temperature was 50°C with xylanase activity of 11.17±0.33 U/mL. The identification results showed that Actinomyces ATG 70 belongs to Gram-positive bacteria, with a positive catalase test, white colonies, cream-colored aerial mycelium, and cream-colored pigmentation. The spore structure consists of long chains of conidia, streptococcus-shaped cells, and can form aerial mycelium, thus the isolate belongs to the genus Streptomyces.
Co-Authors AA Sudharmawan, AA Adi Sucipto, Adi Agung Nugroho Puspito Ajeng Maharani Sri Pananjung Ajeng Maharani Sri Pananjung, Ajeng Maharani Arif Mohammad Siddiq Arif Setiawan Ayuningtyas, Tantri Raras Azhari, Fitri Dina Amalia Syahidah DINA FITRIYAH Dwi Setyati Eka Putri, Khilwiyah Esti Utarti Evi Umayah Ulfa Farrennina, Tasya Preira Fuad Bahrul Ulum Hari Sulistyowati Hasanah, Lailiyah Maulidatul Ika Oktavianawati Izzay Afkarina Kahar Muzakhar Kartika Senjarini Kholiq, Nur Laensugi, Agatha Dei Gratia Lailiyah Maulidatul Hasanah Lelono, Asmoro Lina Nurita Luthfiah Luthfiah Mukhamad Su'udi Puspito, Agung Nugroho Putri, Amelia Fahreza Reza Billa Afifuddin S Siswanto Safira Isti’nafil Islam Siswanto Siswanto Siswanto Siswanto Su'udi, Mukhamad Suharjono Suharjono Suksma, Nadhea Ayu Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Sutoyo Tabah Wibisono, Hariyo Tanfidz Alishlah, Tanfidz Umayah Ulfa, Evi Wicaksono, Bagus Aji Wimbaningrum, Retno Winarsa, Rudju