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All Journal Jurnal Penelitian Saintek Jurnal Biologi Udayana Jurnal Sains Dasar AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains JURNAL BIOLOGI INDONESIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA AL-HAYAT: Journal of Biology and Applied Biology Jurnal Mangifera Edu Bioscientist : Jurnal Ilmiah Biologi Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Kingdom (The Journal of Biological Studies) IJOBI Biosaintifika: Journal of Biology & Biology Education
Paramita Cahyaningrum Kuswandi
Jurusan Pendidikan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Neuroscience Physics Public Health Social Sciences

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In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
Trend of genetics learning media research: A bibliometric analysis (1968-2024) Isnaeni, Bekti; Paidi, Paidi; Mualimin, Mualimin; Kuswandi, Paramita Cahyaningrum
Jurnal Mangifera Edu Vol 10 No 1 (2025): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v10i1.217

Abstract

The use of learning media difficulty in comprehending complex material, the inability to effectively visualize microscopic objects that require specialized teaching aids or interactive media. This descriptive qualitative research, employing a bibliometric approach, aims to analyze research trends on learning media in genetics from 1964 to 2024. The analysis focuses on the volume and distribution of publications, the contributing countries, and the institutional affiliations, as well as key journals in the field. The research subject is learning media in genetics courses. Data collected through the Scopus website, using the keywords “genetic,” “learning,” and “media,” yielded 1,135 results, which were analyzed using R and Biblioshiny software. The trend in research on genetics learning media has increased year by year, with a sharp increase from 2017 to 2023. This result indicating that the research trend reached its peak in that year. The USA and China are the countries that contribute the most to publishing articles, suggesting potential for collaboration with various countries. The most active affiliation, with a total of 72 articles, is the University of California. The journal that contributes the most to genetics learning media is the Journal of Behavior Genetics. This research can serve as a reference for future researchers interested in studying genetics learning media, with the recommendation to combine multiple applications, such as Biblioshiny with Vosviewer, to ensure more precise and more valid findings. Additionally, using more specific keywords, such as e-modules, virtual reality (VR), augmented reality (AR), or game-based learning, is advised.
Parental Diet Exposure and High-Sugar-Fat Intake Effect on Glucose, Triglyceride and Cholesterol Hemolymph Level of Drosophila melanogaster across Five Generations Pertiwi, Kartika Ratna; Kuswandi, Paramita Cahyaningrum; Anazifa, Rizqa Devi; Zain, Haniza Hanim Mohd
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.23709

Abstract

Metabolic syndrome (MetS) is influenced by parental traits and diet. Drosophila melanogaster is a potential disease model organism, sharing physiology and genetic similarities with humans. Previous research had focused on Drosophila melanogaster as a model organism for obesity and diabetes, but not for MetS. This research aimed to determine the effect of both parental diet and high sugar fat (HSF) intake on glucose, cholesterol, and triglyceride hemolymph levels of Drosophila melanogaster. Wild flies were purified in either control (standard) or MetS media (extra 3% sucrose and palm oil). Seventy-five pairs were divided into 5 groups, according to parental origin and feeding media, and maintained in five generations (F1-F5). Glucose, triglyceride, and cholesterol levels were measured using a colorimetric assay in three replications of each generation per group. Glucose, triglyceride, and cholesterol levels were significantly different in all treatment than control groups, and across generations in each group (p<0.05). Higher glucose and triglyceride levels appeared in the youngest generation (F5) of all groups, and in the flies reared on HSF diets. Maternal HSF-exposed groups demonstrated a more pronounced impact of parental metabolic-state on the glucose and triglyceride levels of the earlier generation. These findings highlight that parental exposure to HSF and prolonged HSF intake independently and synergistically lead to persistent and amplified metabolic dysregulation across generations. Drosophila melanogaster, modeled in this study, represents a novel experimental organism that is suitable for studying the epigenetics of MetS, gaining more consideration for the metabolic health consequences of long-term dietary habits and parental metabolic-state.
Analisis Representasi Visual Buku Teks Genetika Tentang DNA Isnaeni, Bekti; Kuswandi, Paramita Cahyaningrum; Subiantoro, Agung Wijaya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16398

Abstract

This study aims to analyze the visual representation aspects and level of representation in genetics textbooks related to DNA material. This study is descriptive qualitative with two genetics textbooks as the research objects. The analysis technique used is visual representation function analysis and level of representation. The results of the analysis show that visual representation is dominated by illustrative functions (70-75%), followed by complementary functions (23-25%), while explanatory functions only appear 5%, and decorative functions are not found. The level of DNA topic representation falls under the molecular level of representation. This finding indicates that the visual representations in both genetics textbooks are predominantly illustrative, followed by complementary functions, while explanatory functions appear only to a limited extent, and decorative functions are absent. This analysis provides valuable insight into the quality of the textbooks and their relevance to potential student misconceptions. The implication of this study is that it can serve as a reference for authors of genetics textbooks, particularly in the topic of DNA, to pay closer attention to the balance of visual representation functions, ensuring that no single function dominates and that the visuals more effectively support students’ conceptual understanding.
Co-Authors ,, Wirdateti ,, Wirdateti Agung Wijaya Subiantoro Anazifa, Rizqa Devi Anazifa, Rizqa Devi Anggi Tias Pratama Arief Priyadi Asih, Ni Putu Sri Dwi Ayuningtyas Evy Yulianti Fajar Prasetya Ibnu Pratomo isnaeni, bekti Ixora Sartika Mercuriani Kartika Ratna Pertiwi Khan, Farwa Hafeez Lailani, Zhafira Istiqla Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Lili Sugiyarto Md Hoirul Azri Ponari Mohd Syafik Mohamad Hamdan Mohd Syahmi Salleh Mualimin Mualimin Muhamad Fahmi Yunus Paidi Paidi Pratiwi, Risha Amilia Rizka Anisa Rennytasari Rizka Apriyani Putri Selma Nafilatul Husna Shafila Sahnaz Suratsih Suratsih Tien Aminatun Tutiek Rahayu Wiharyanti, Risma Wiharyanti, Risma Wulandari*, Novia Dian Kartika Wulandari, Sri Wijayanti Wulandari, Sri Wijayanti Zain, Haniza Hanim Mohd