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All Journal Jurnal Penelitian Saintek Jurnal Biologi Udayana AL KAUNIYAH Jurnal Sains Dasar AGRIVITA, Journal of Agricultural Science Journal of Tropical Biodiversity and Biotechnology Scientiae Educatia: Jurnal Pendidikan Sains JURNAL BIOLOGI INDONESIA Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Jurnal Pengabdian Masyarakat MIPA dan Pendidikan MIPA AL-HAYAT: Journal of Biology and Applied Biology Jurnal Mangifera Edu Jurnal Pedagogi dan Pembelajaran Bioscientist : Jurnal Ilmiah Biologi Jurnal Pendidikan Sains Indonesia (Indonesian Journal of Science Education) Kingdom (The Journal of Biological Studies) IJOBI Biosaintifika: Journal of Biology & Biology Education
Paramita Cahyaningrum Kuswandi
Jurusan Pendidikan Biologi, Fakultas Matematika Dan Ilmu Pengetahuan Alam, Universitas Negeri Yogyakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Decision Sciences, Operations Research & Management Education Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Neuroscience Physics Public Health Social Sciences Other

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In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

Abstract

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA
Trend of genetics learning media research: A bibliometric analysis (1968-2024) Isnaeni, Bekti; Paidi, Paidi; Mualimin, Mualimin; Kuswandi, Paramita Cahyaningrum
Jurnal Mangifera Edu Vol 10 No 1 (2025): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v10i1.217

Abstract

The use of learning media difficulty in comprehending complex material, the inability to effectively visualize microscopic objects that require specialized teaching aids or interactive media. This descriptive qualitative research, employing a bibliometric approach, aims to analyze research trends on learning media in genetics from 1964 to 2024. The analysis focuses on the volume and distribution of publications, the contributing countries, and the institutional affiliations, as well as key journals in the field. The research subject is learning media in genetics courses. Data collected through the Scopus website, using the keywords “genetic,” “learning,” and “media,” yielded 1,135 results, which were analyzed using R and Biblioshiny software. The trend in research on genetics learning media has increased year by year, with a sharp increase from 2017 to 2023. This result indicating that the research trend reached its peak in that year. The USA and China are the countries that contribute the most to publishing articles, suggesting potential for collaboration with various countries. The most active affiliation, with a total of 72 articles, is the University of California. The journal that contributes the most to genetics learning media is the Journal of Behavior Genetics. This research can serve as a reference for future researchers interested in studying genetics learning media, with the recommendation to combine multiple applications, such as Biblioshiny with Vosviewer, to ensure more precise and more valid findings. Additionally, using more specific keywords, such as e-modules, virtual reality (VR), augmented reality (AR), or game-based learning, is advised.
Parental Diet Exposure and High-Sugar-Fat Intake Effect on Glucose, Triglyceride and Cholesterol Hemolymph Level of Drosophila melanogaster across Five Generations Pertiwi, Kartika Ratna; Kuswandi, Paramita Cahyaningrum; Anazifa, Rizqa Devi; Zain, Haniza Hanim Mohd
Biosaintifika: Journal of Biology & Biology Education Vol. 17 No. 2 (2025): August 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v17i2.23709

Abstract

Metabolic syndrome (MetS) is influenced by parental traits and diet. Drosophila melanogaster is a potential disease model organism, sharing physiology and genetic similarities with humans. Previous research had focused on Drosophila melanogaster as a model organism for obesity and diabetes, but not for MetS. This research aimed to determine the effect of both parental diet and high sugar fat (HSF) intake on glucose, cholesterol, and triglyceride hemolymph levels of Drosophila melanogaster. Wild flies were purified in either control (standard) or MetS media (extra 3% sucrose and palm oil). Seventy-five pairs were divided into 5 groups, according to parental origin and feeding media, and maintained in five generations (F1-F5). Glucose, triglyceride, and cholesterol levels were measured using a colorimetric assay in three replications of each generation per group. Glucose, triglyceride, and cholesterol levels were significantly different in all treatment than control groups, and across generations in each group (p<0.05). Higher glucose and triglyceride levels appeared in the youngest generation (F5) of all groups, and in the flies reared on HSF diets. Maternal HSF-exposed groups demonstrated a more pronounced impact of parental metabolic-state on the glucose and triglyceride levels of the earlier generation. These findings highlight that parental exposure to HSF and prolonged HSF intake independently and synergistically lead to persistent and amplified metabolic dysregulation across generations. Drosophila melanogaster, modeled in this study, represents a novel experimental organism that is suitable for studying the epigenetics of MetS, gaining more consideration for the metabolic health consequences of long-term dietary habits and parental metabolic-state.
Analisis Representasi Visual Buku Teks Genetika Tentang DNA Isnaeni, Bekti; Kuswandi, Paramita Cahyaningrum; Subiantoro, Agung Wijaya
Bioscientist : Jurnal Ilmiah Biologi Vol. 13 No. 3 (2025): September
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v13i3.16398

Abstract

This study aims to analyze the visual representation aspects and level of representation in genetics textbooks related to DNA material. This study is descriptive qualitative with two genetics textbooks as the research objects. The analysis technique used is visual representation function analysis and level of representation. The results of the analysis show that visual representation is dominated by illustrative functions (70-75%), followed by complementary functions (23-25%), while explanatory functions only appear 5%, and decorative functions are not found. The level of DNA topic representation falls under the molecular level of representation. This finding indicates that the visual representations in both genetics textbooks are predominantly illustrative, followed by complementary functions, while explanatory functions appear only to a limited extent, and decorative functions are absent. This analysis provides valuable insight into the quality of the textbooks and their relevance to potential student misconceptions. The implication of this study is that it can serve as a reference for authors of genetics textbooks, particularly in the topic of DNA, to pay closer attention to the balance of visual representation functions, ensuring that no single function dominates and that the visuals more effectively support students’ conceptual understanding.
EFFECTIVENESS OF MAJAPAHIT LEAF EXTRACT (Crescentia cujete L.) AS A NATURAL PESTICIDE FOR BANANA LEAF ROLLER CATERPILLAR (Erionota thrax L). Pratomo, Ibnu; Aminatun, Tien; Kuswandi, Paramita Cahyaningrum
Indonesian Journal of Bioscience (IJOBI) Vol. 1 No. 1 (2023): Indonesian Journal of Bioscience (IJOBI)
Publisher : Universitas Negeri Yogyakarta in collaboration with Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/ijobi.v1i1.106

Abstract

This study aims to determine the effectiveness of Majapahit leaf extract (Crescentia cujete L.) on the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.), and also the optimal concentration that affects the feeding activity and mortality of the banana leaf roller caterpillar (Erionota thrax L.). This research is a completely randomized design (CRD) experiment with 4 treatments and 3 replicates. In thos study, 60 third instar larvae of Erionota thrax L. were taken using the transect method from several villages in Purworejo district, Central Java. The pesticide from Majapahit leaves was made through a simple extraction method with 4 concentrations: 0%, 25%, 30% and 35%. The results showed that Majapahit leaf extract had an effect on feeding activity and mortality of the banana leaf rolling caterpillar. The optimal concentration of Majapahit leaf extract that affects feeding activity and pest mortality was 35%.
A Transgenerational Effects of Oil Supplemented Diets on Fruit Flies (Drosophila melanogaster) Progenies Kuswandi, Paramita Cahyaningrum; Pertiwi, Kartika Ratna; Aji, Ibnu Kholdun Sukma
Al-Kauniyah: Jurnal Biologi Vol. 18 No. 1 (2025): AL-KAUNIYAH JURNAL BIOLOGI
Publisher : Department of Biology, Faculty of Science and Technology, Syarif Hidayatullah State Islami

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/kauniyah.v1i1.35362

Abstract

AbstractThe causes of obesity are multifactorial, one of which is the parental diet. Fruit fly (Drosophila melanogaster) has homologous genes with humans and is a potential model organism for research on human diseases. This study aims to examine the transgenerational effects of a modified high-fat diet on the number of progenies, sex ratio, and body weight in eight generations of fruit flies. Four types of diets consisted of different fat compositions were used banana, cornmeal, cornmeal + 3% virgin coconut oil (VCO), and cornmeal + 3% palm oil. Data were analyzed using SPSS with ANOVA and DMRT post hoc analysis. Results showed significant differences (P <0.05) in the number of progenies between the diet without oil and with added oil. A higher number of progenies were found in the diet without oil with an average of 85 flies (banana diet) and 85.04 flies (cornmeal diet) compared to the results in the oil diet with 19 flies (cornmeal + 3% VCO) and 76.67 flies (cornmeal + 3% palm oil)The sex ratio and the body weight were not significantly different between each diet, suggesting that there are other factors involved. There were significant differences between the early and late generations for number of progenies and body weight, but not for sex ratio. The VCO was difficult to mix in the media so palm oil has the potential to be used for further investigations to find a better formula for high-fat diet food sources in an obesity study using fruit flies.AbstrakPenyebab terjadinya obesitas bersifat multifaktorial, salah satunya adalah pola makan orang tua. Lalat buah (Drosophila melanogaster) memiliki gen yang homolog dengan manusia dan merupakan organisme model yang potensial untuk penelitian penyakit manusia. Penelitian ini bertujuan untuk menguji pengaruh transgenerasi pola makan tinggi lemak yang dimodifikasi terhadap jumlah keturunan, rasio jenis kelamin, dan berat badan pada delapan generasi lalat buah. Empat jenis pakan dengan komposisi lemak berbeda digunakan yaitu pisang, tepung jagung, tepung jagung + 3% Virgin coconut oil(VCO) dan tepung jagung + 3% minyak sawit. Data dianalisis menggunakan SPSS dengan analisis post hoc ANOVA dan DMRT. Hasil penelitian menunjukkan perbedaan yang signifikan (P <0,05) pada jumlah keturunan, khususnya antara diet tanpa minyak dengan diet dengan minyak. Jumlah progeni yang lebih banyak terdapat pada diet tanpa minyak dengan rata-rata 85 lalat (diet pisang) dan 85,04 lalat (diet tepung jagung) dibandingkan dengan hasil pada diet minyak dengan 19 lalat (tepung jagung + 3% VCO) dan 76,67 lalat (tepung jagung + 3% minyak sawit). Rasio jenis kelamin dan berat badan tidak berbeda secara signifikan antara masing-masing pola makan, hal ini menunjukkan bahwa ada faktor lain yang terlibat. Terdapat perbedaan yang signifikan antara generasi awal dan akhir dalam hal jumlah keturunan dan berat badan, namun tidak untuk rasio jenis kelamin. VCO sulit untuk dicampurkan dalam media sehingga minyak sawit berpotensi digunakan untuk penelitian lebih lanjut untuk menemukan formula sumber makanan diet tinggi lemak yang lebih baik dalam studi obesitas menggunakan lalat buah.
Efektivitas Fungisida dan Cuka untuk Sterilisasi Eksplan Sirih Hitam (Piper betle L. var Nigra) Kuswandi, Paramita Cahyaningrum; Prasetya, Fajar
Bioscientist : Jurnal Ilmiah Biologi Vol. 12 No. 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9901

Abstract

Black betel (Piper betle L. var. Nigra) can be found in Indonesia and has been proven to have antimicrobial activity so it has the potential to be used as a medicinal plant. Tissue culture can be used to produce seeds that are the same as the parent plant in large, uniform quantities and to produce secondary metabolites from cell suspension cultures. The sterilization method is a very important initial stage for in vitro plant growth, so optimizing sterilization is the aim of this research. The planting materials used are black betel leaves and nodes. Optimization of the sterilization method was carried out using variations in materials and sterilization time duration. The ingredients used were dish soap, bactericide, fungicide, sodium hypochlorite solution, vinegar, alcohol, and Plant Preservative Mixture (PPM). The medium for leaf explants is MS with 2,4-D and for nodes was MS with BAP. Data on the number of contaminated explants were analyzed using the SPSS program. The results of analysis of variance (ANOVA) showed that the optimal sterilization of black betel leaves was using a fungicide solution for 30 minutes, 5% sodium hypochlorite solution for 10 and 15 minutes, vinegar for 7 minutes, 70% alcohol for 10 minutes and PPM for 1 minute with a 22.1% of explants contaminated. Sterilization by rubbing the nodes under running water and the use of vinegar to sterilize black betel nodia resulted in a significantly lower percentage of contamination (73.3%, P<0.05) compared to the method where the leaves were not cleaned by rubbing under running water and no vinegar.
Agrowisata belimbing Karangsari, potensi lokal Kota Blitar sebagai sumber belajar biologi untuk meningkatkan literasi sains peserta didik Sahnaz, Shafila; Kuswandi, Paramita Cahyaningrum
Jurnal Penelitian Pendidikan IPA Vol 9 No 8 (2023): August
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i8.3888

Abstract

The utilization of local potential as a biology learning source is still not maximized. This research focuses on integrating the local potential of Blitar, namely Karangsari’s Starfruit Agrotourism which was developed into a student worksheet app to increase students' science literacy. Karangsari’s Starfruit Agrotourism can be used as a learning resource on biodiversity material by discussing the diversity of starfruit (Averrhoa carambola) varieties, the use of starfruit, and efforts to preserve starfruit. Utilizing local potential and implementing the Sustainable Development Goals (SDG) in biology learning aligns with the Merdeka Belajar curriculum. This study used the ADDIE model which consists of analysis, design, development, implementation, and evaluation stages. The final product of this development research is a student worksheet app implemented to enhance science literacy in class X of students at SMAN 1 Blitar. Data was collected with needs questionnaires, teacher interviews, assessment questionnaires for material experts, media experts, biology teachers, student response questionnaires, and science literacy skills tests. The data obtained were statistically analyzed tests between subjects. Research results concluded as Karangsari’s starfruit agrotourism applied as the materials of the student worksheet app was declared feasible by experts, there are differences in science literacy between students who had taken part in learning while using the worksheet and students who did not, and the effect/contribution given by student worksheet apps to science literacy is 58%.
PteridophytaE-Encyclopedia of Kelud Mountain to Study of the Diversity of Fern Species in the Environment on the RICOSRE Model to Improve Student’s Information Literacy Ayuningtyas, Dwi; Kuswandi, Paramita Cahyaningrum
Jurnal Penelitian Pendidikan IPA Vol 10 No 1 (2024): January
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v10i1.5917

Abstract

The low level of information literacy and the lack of utilization of local potential is one of the reasons to develop the learning innovations. One of innovation is utilize local potential as a learning resource in the form of an e-encyclopedia and integrate it with the RICOSRE learning model. This research aims to determine the feasibility, practicality, and effectiveness from the pteridophyta e-encyclopedia of Kelud Mount for studying the diversity of fern species on RICOSRE model to increase information literacy. This research uses the development research with the ADDIE design and implemented in class X of students at SMA Negeri 1 Talun. The instruments research were questionnaires and information literacy rubrics. The data result was analyzed using the percentage equation, while information literacy data was analyzed with N-Gain equation and Independent Sample T-Test. The research results show that the pteridophyta e-encyclopedia are feasible by lecturer and stated to be practical by teachers and students. The conclusion is the information literacy of students who use e-encyclopaedia’s in RICOSRE learning is proven to be higher than students who do not use it.
Development of Google Sites Problem-Based Learning Differentiated on Biodiversity Materials to Improve Students Interest and Concept Understanding Arsyada, Tasya; Kuswandi, Paramita Cahyaningrum; Nisa, Khoirun
Jurnal Pendidikan Sains Indonesia Vol 13, No 4 (2025): OCTOBER 2025
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v13i4.49164

Abstract

Learning biodiversity in biology education often presents challenges because the concepts are abstact and difficult to connect with real-life contexts. Coventional learning media generally lack interactivity and fail to accommodate diverse student needs, resulting in low engagement and limited conceptual understanding. Therefore, this study aims to develop, validate, and evaluate the effectiveness of google sites-based learning media integrated with a differentiated problem-based learning (PBL) model for biodiversity material. The research employed the RD method, utilizing the ADDIE model, with 72 students from SMA Negeri 1 Sewon, divided into experimental (X-9) and control (X-10) classes. Data were collected through interviews, needs analysis, validation by experts, teacher and student assessments, concept understanding tests, and learning interest questionnaires. Data analysis employed descriptive techniques and Manova. The results indicated that the developed google sites-based PBL media is highly feasible, practical, and effective. Expert validation confirmed that the content aligns with competencies and learning objectives, presented through structured materials and user friendly design. Teachers and students reported positive responses toward its practicality and engagement. The integration of differentiated instruction within the PBL framework significantly enhanced students learning interest and conceptual understanding. Therefore, google sites-based PBL media can serve as an innovative and inclusive digital learning tool that supports meaningful and active biology learning experiences
Co-Authors ,, Wirdateti ,, Wirdateti Agung Wijaya Subiantoro Aji, Ibnu Kholdun Sukma Aminatun, Tien Anafiza, Rizqa Devi Arief Priyadi Arsyada, Tasya Asih, Ni Putu Sri Ayuningtyas, Dwi Damayanti, Alfiya Evy Yulianti Fajar Prasetya isnaeni, bekti Ixora Sartika Mercuriani Kartika Ratna Pertiwi Khan, Farwa Hafeez Khoirun Nisa Lailani, Zhafira Istiqla Lili Sugiyarto Md Hoirul Azri Ponari Mohd Syafik Mohamad Hamdan Mohd Syahmi Salleh Mualimin Mualimin Muhamad Fahmi Yunus Paidi Paidi Prasetya, Fajar Pratama, Anggi Tias Pratiwi, Risha Amilia Pratomo, Ibnu Rizka Anisa Rennytasari Rizka Apriyani Putri Sahnaz, Shafila Selma Nafilatul Husna Suratsih Suratsih Tutiek Rahayu Wiharyanti, Risma Wiharyanti, Risma Wulandari*, Novia Dian Kartika Wulandari, Sri Wijayanti Wulandari, Sri Wijayanti Zain, Haniza Hanim Mohd