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THE EFFECT OF SEVERAL CONCENTRATIONS OF GLYCINE AMINO ACID ADDITIONS IN MS MEDIUM TOWARS CALLUS GROWTH OF KONJAC (Amorphophallus muelleri) BY in vitro CULTURE Rizka Anisa Rennytasari; Paramita Cahyaningrum Kuswandi
Kingdom (The Journal of Biological Studies) Vol 8, No 2 (2022): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v8i2.18153

Konjac (Amorphophallus muelleri) is a plant that can be a source of carbohydrates and has high economic value. To accelerate the fulfillment of porang seeds in order to support maximum economic potential, it is necessary to develop alternative propagation techniques,which is in vitro culture. This study aimed to determine the effect of adding the glycine amino acid to MS medium on the growth of konjac callus and to determine the best concentration for callus growth in konjac plant tissue culture. This research was using frog bulb (bulbil) for the explant. In this study used a completely randomized design (CRD) with glycine amino acid concentrations from 0 mg/L, 1 mg/L, 2 mg/L, 3 mg/L, 4 mg/L, dan 5 mg/L with 3 replications. Data were analyzed using SPSS software with one way ANAVA and further test using DMRT 5%.The results showed that the addition of the glycine amino acid to MS medium affected the callus growth of konjac plant tissue culture based on the analysis of variance (ANAVA) test. The best concentration used based on the Duncan Multiple Range Test (DMRT) 5%, was 4 mg/L with the average callus appearance time of 3.06 wac and the average bending time of 2.1 wac.

Pengaruh waktu induksi dan ZPT terhadap pertumbuhan kalus kotiledon kacang panjang (Vigna unguiculata subsp. sesquipedalis) Paramita Cahyaningrum Kuswandi; Selma Nafilatul Husna
Jurnal Biologi Udayana Vol 27 No 2 (2023): JURNAL BIOLOGI UDAYANA
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/JBIOUNUD.2023.v27.i02.p07

Tanaman kacang panjang adalah komoditas pertanian yang sangat berpotensi untuk dikembangkan karena mudah dibudidayakan dan pangsa pasar cukup tinggi, namun masih ada permasalahan dalam memenuhi kebutuhan benih dan masih rentan terhadap hama dan penyakit. Oleh karena itu perlu dilakukan upaya peningkatan produksi dan induksi variasi kacang panjang. Salah satu metode yang dapat digunakan adalah kultur jaringan untuk induksi variasi somaklonal dan/atau pembentukan tanaman transgenik. Penelitian ini bertujuan untuk mengetahui pengaruh variasi waktu inisiasi kalus dan pengaruh pemberian ZPT terhadap pertumbuhan kalus eksplan kotiledon kacang panjang. Sterilisasi biji dilakukan dengan menggunakan detergen, natrium hipoklorit 10%, alkohol 70% dan akuades steril. Hasil penelitian menunjukkan bahwa variasi waktu inisiasi kalus pada media MS dengan penambahan ZPT 2,4-D serta penggunaan beberapa variasi ZPT pada subkultur mampu menginisiasi kalus dan mempengaruhi ukuran kalus, warna kalus, serta persentase browning eksplan kotiledon kacang panjang. Waktu inisiasi kalus 4 minggu dengan kombinasi konsentrasi BAP 2 ppm dan IAA 0,1 ppm menghasilkan ukuran kalus paling besar dan warna kalus putih kecoklatan. Penambahan ZPT saat subkultur memiliki persentase browning yang lebih kecil dari perlakuan tanpa ZPT.

Nutrient Requirement and Fertilizer Management for Agarwood Plantations: A Review Muhamad Fahmi Yunus; Mohd Syahmi Salleh; Md Hoirul Azri Ponari; Paramita Cahyaningrum Kuswandi; Mohd Syafik Mohamad Hamdan
AGRIVITA Journal of Agricultural Science Vol 46, No 2 (2024)
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v46i2.4437

Agarwood is a highly valued fragrant wood and has been traded for its economic and medicinal value. The resin in agarwood is used to produce high value downstream products such as fragrances, oils, medicines, teas, cosmetic carvings, and luxury goods where the demand for these products remains consistent. Most of the agarwood-producing trees come from the genera of Aquilaria and Gyrinops, which belong to the Thymelaeaceae (Malvales) family. In addition, Aquilaria malaccensis and Gyrinops versteegii are the most cultivated agarwood producing trees in Malaysia and Indonesia, respectively. Nevertheless, the survival of the species is threatened by the overharvesting of the forest, which leads to strict regulation by the exporting nations for international trade. Interestingly, agarwood plantation is a viable option which able to generate income for local population without causing any harm to the ecosystems. However, a lack of research on seedling preparation for large-scale plantations, and the optimum nutrient management practices have slowed down the agarwood industry. Hence, this review article will highlight the most effective strategy for enhancing agarwood cultivation by focusing on proper nutrient management to boost agarwood production.

PENGARUH PENAMBAHAN BAP TERHADAP INDUKSI KALUS TANAMAN PORANG SECARA IN VITRO Lailani, Zhafira Istiqla; Kuswandi, Paramita Cahyaningrum
Kingdom: The Journal of Biological Studies Vol 9, No 1 (2023): Kingdom: The Journal of Biological Studies
Publisher : Universitas Negeri Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21831/kingdom.v9i1.18481

Abstrak. Porang merupakan sumber karbohidrat yang bernilai ekonomi tinggi. Untuk memenuhi permintaan porang sebagai produk diversifikasi pangan maupun produk ekspor, perlu dukungan ketersediaan benih dan budidaya yang memadai. Perbanyakan secara in vitro menjadi alternatif dalam pemenuhan bibit porang yaitu melalui metode kultur kalus dengan menggunakan zat pengatur tumbuh BAP pada media MS. Penelitian ini bertujuan untuk mengetahui pengaruh penambahan BAP terhadap induksi kalus tanaman porang (Amorphophallus muelleri). Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) dengan beberapa konsentrasi BAP yang terdiri dari 6 level (0, 1, 2, 3, 4, 5) mg/liter sebanyak 6 ulangan. Data yang diperoleh dianalisis dengan uji one way ANOVA dan uji lanjut menggunakan DMRT 5%. Hasil penelitian menunjukkan bahwa penambahan BAP pada media MS berpengaruh pada pertumbuhan kalus kultur jaringan tanaman porang berdasarkan uji one way ANOVA. Konsentrasi 2 mg/L BAP merupakan konsentrasi yang paling cepat dalam menginduksi pertumbuhan kalus dengan rata-rata waktu pelengkungan kalus 1,450 mst dan rata-rata waktu munculnya kalus 2,633 mst.Kata Kunci: induksi kalus; BAP; Amorphophallus muelleri.

The Complete Chloroplast Genome of Medinilla tapete-magicum (Melastomataceae) from Sulawesi, Indonesia Priyadi, Arief; Kuswandi, Paramita Cahyaningrum; Yulianti, Evy; Pratiwi, Risha Amilia; Asih, Ni Putu Sri
Journal of Tropical Biodiversity and Biotechnology Vol 9, No 2 (2024): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jtbb.87932

In this study, the genome of an endemic Sulawesi’s plant, Medinilla tapete-magicum was sequenced using Illumina NextSeq 500 and assembled the whole chloroplast genome. Results showed that the cpGenome is 155,602 bp in size with typical quadripartite structure of a large single copy (LSC) region (85,409 bp), a short single copy (SSC) region (16,629 bp), and a pair of inverted repeats (IRs) regions (26,782 bp). The cpGenome is composed of 132 genes, which consists of 87 protein coding genes, 37 tRNAs, and 8 rRNAs. The sliding window analyses showed that psbB-psbH and ndhF-rpl32 can potentially be used as markers. Microsatellite motifs of mononucleotide A and T dominated in the cpGenome. The phylogenetic trees from the concatenated 76 shared protein coding gene sequences showed the Medinilla clade was monophyletic and M. tapete-magicum is a sister species in the SE Asian clade which contain M. magnifica and M. speciosa.

Efektivitas Fungisida dan Cuka untuk Sterilisasi Eksplan Sirih Hitam (Piper betle L. var Nigra) Paramita Cahyaningrum Kuswandi; Fajar Prasetya
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.9901

Black betel (Piper betle L. var. Nigra) can be found in Indonesia and has been proven to have antimicrobial activity so it has the potential to be used as a medicinal plant. Tissue culture can be used to produce seeds that are the same as the parent plant in large, uniform quantities and to produce secondary metabolites from cell suspension cultures. The sterilization method is a very important initial stage for in vitro plant growth, so optimizing sterilization is the aim of this research. The planting materials used are black betel leaves and nodes. Optimization of the sterilization method was carried out using variations in materials and sterilization time duration. The ingredients used were dish soap, bactericide, fungicide, sodium hypochlorite solution, vinegar, alcohol, and Plant Preservative Mixture (PPM). The medium for leaf explants is MS with 2,4-D and for nodes was MS with BAP. Data on the number of contaminated explants were analyzed using the SPSS program. The results of analysis of variance (ANOVA) showed that the optimal sterilization of black betel leaves was using a fungicide solution for 30 minutes, 5% sodium hypochlorite solution for 10 and 15 minutes, vinegar for 7 minutes, 70% alcohol for 10 minutes and PPM for 1 minute with a 22.1% of explants contaminated. Sterilization by rubbing the nodes under running water and the use of vinegar to sterilize black betel nodia resulted in a significantly lower percentage of contamination (73.3%, P<0.05) compared to the method where the leaves were not cleaned by rubbing under running water and no vinegar.

Analysis of Students Critical Thinking Skills in Nervous System Learning Based on the Level of School Favoritism in Sleman Regency Wulandari*, Novia Dian Kartika; Kuswandi, Paramita Cahyaningrum; Khan, Farwa Hafeez
Jurnal Pendidikan Sains Indonesia Vol 12, No 4 (2024): OCTOBER 2024
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24815/jpsi.v12i4.39205

Critical thinking skills (CTS) are importante cognitive ability to be improved by students in solving problems in the future. This ability needs to be trained in learning at school to equip students in the 21st century. Problems with students' CTS can lead to a decrease in the quality of education and the quality of students. The purpose of this study was to analyze the profile of CTS of high school students at Sleman Regency in biology learning, especially on the topic of the nervous system. This type of research is descriptive research. The research was conducted in six high schools throughout Sleman Regency. The population in this study is grade XI students in six high schools consisting of two most favorite category schools, two favorite category schools, and two less favorite category schools in Sleman Regency. Sampling using random purposive sampling method from six high schools in Sleman Regency totaling 216 students. The data collection technique using the question test method is using test instrument sheet. The data analysis technique used quantitative descriptive. The results showed that the CTS of high school students at Sleman Regency in biology learning reached an average of 46.6% in the medium category. Schools with most favorite categories have the highest CTS for all aspects tested compared to favorite and less favorite category schools. Meanwhile, the lowest CTS is the less favorite category of schools. This shows that schools with most favorite categories have better CTS for all aspects

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

In Silico Approach of DNA Barcoding in Cattleya Orchid using nrDNA and matK Markers Wiharyanti, Risma; Mercuriani, Ixora Sartika; Yulianti, Evy; Kuswandi, Paramita Cahyaningrum
Al-Hayat: Journal of Biology and Applied Biology Vol. 8 No. 1 (2025)
Publisher : Fakultas Sains dan Teknologi, UIN Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/ah.v8i1.25671

The Cattleya genus is one of the most commercially valuable orchids. Identifying these orchids based on morphological and phenotypic traits has been challenging. Accurate identification is crucial for distinguishing between native and hybrid species and determining the protected status of certain Cattleya orchids. This research explored potential markers using nrDNA and matK locus for DNA barcoding of the Cattleya genus through an in silico approach. All sequences were collected from the NCBI and analyzed using ClustalX2 for alignment, BioEdit for conversion, and MEGA11 for phylogenetic tree construction. The results revealed that nrDNA showed higher genetic variation compared to matK. However, neither phylogenetic tree could discriminate species precisely, as some Cattleya species were positioned closer to the outgroup. These findings can be used as a reference to support identification of Cattleya using molecular marker. Keywords: Cattleya, matK, molecular barcode, nrDNA

Trend of genetics learning media research: A bibliometric analysis (1968-2024) Isnaeni, Bekti; Paidi, Paidi; Mualimin, Mualimin; Kuswandi, Paramita Cahyaningrum
Jurnal Mangifera Edu Vol 10 No 1 (2025): Jurnal Mangifera Edu
Publisher : Universitas Wiralodra

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31943/mangiferaedu.v10i1.217

The use of learning media difficulty in comprehending complex material, the inability to effectively visualize microscopic objects that require specialized teaching aids or interactive media. This descriptive qualitative research, employing a bibliometric approach, aims to analyze research trends on learning media in genetics from 1964 to 2024. The analysis focuses on the volume and distribution of publications, the contributing countries, and the institutional affiliations, as well as key journals in the field. The research subject is learning media in genetics courses. Data collected through the Scopus website, using the keywords “genetic,” “learning,” and “media,” yielded 1,135 results, which were analyzed using R and Biblioshiny software. The trend in research on genetics learning media has increased year by year, with a sharp increase from 2017 to 2023. This result indicating that the research trend reached its peak in that year. The USA and China are the countries that contribute the most to publishing articles, suggesting potential for collaboration with various countries. The most active affiliation, with a total of 72 articles, is the University of California. The journal that contributes the most to genetics learning media is the Journal of Behavior Genetics. This research can serve as a reference for future researchers interested in studying genetics learning media, with the recommendation to combine multiple applications, such as Biblioshiny with Vosviewer, to ensure more precise and more valid findings. Additionally, using more specific keywords, such as e-modules, virtual reality (VR), augmented reality (AR), or game-based learning, is advised.

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