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Christina Salaki
Universitas Sam Ratulangi
Religion Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Library & Information Science Mathematics Medicine & Pharmacology Physics Public Health Social Sciences Other

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Analisis Keanekaragaman Isolat Bacillus thuringiensis yang Patogenik terhadap Serangga Hama Kubis (Crocidolomia binotalis) dengan Pendekatan Sistematika Numerik Salaki, Christina L.; Situmorang, Jesmandt; Sembiring, Langkah; Handayani, Niken
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (540.381 KB) | DOI: 10.24002/biota.v15i3.2605

Abstract

Diversity of B. thuringiensis (Bt.) isolates pathogenic to C. binotalis was determined by using Numerical Systematic Method. Ten isolates were taken to represent 34 pathogenic isolates along with two reference strains namely B. thuringiensis serovar kurstaki and B. thuringiensis serovar israelensis. The test isolates were examined for 89 phenotypic characters by using convensional method for colonial and cell morphology (37 characters) as well as physiological characteristics (3 characters) but biochemical characterization (49 characters) was conducted by using commercial API-50 CHB procedures. All phenotypic characters existed in one of two mutually exclusive states and were either scored plus (1) of minus (0). The binary data were prepared in Programmer’s File Editor (PFE) software. The data then were analysed by using the Multi Variate statistical Package (MVSP) Plus-Version 3.1 using the Simple Matching Coefficient (SSM). Clustering was achieved using the UPGMA algorithm. The results were presented as dendrograms. It was obtained that the test isolates were clearly assigned to two distinct multimembered clusters defined by 79.6 similarity level (S-level) in the SSM, UPGMA analysis. The two distinct clusters represented by each of two widely known different group of Bt. strains, namely serovar israelensis and serovar kurstaki. The first cluster contained reference strain of B. thuringiensis serovar israelensis, and two of the isolates (Slk2.3, and YPPA1) and the second cluster contained another reference strain of B. thuringiensis serovar kurstaki, and 8 of the isolates. Therefore, it strongly suggested that the application of numerical-fenetic analysis could provide a tool to unravel the strain diversity belong to B. thuringiensis.
PENYEBARAN POPULASI HAMA Paraeucosmetus sp. DI KABUPATEN MINAHASA TENGGARA Salaki, Christina; Senewe, Emmy
EUGENIA Vol 18, No 2 (2012)
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35791/eug.18.2.2012.3563

Abstract

ABSTRACT Insects Paraeucosmetus sp. Brazilians is a destructive pest of rice grain content of the rice plant to mature milk. Population and the level of this pest has spread in several locations rice production centers. This study aims to determine the spread and intensity of pest populations Paraeucosmetus sp. in rice plants by altitude in Southeast Minahasa district (District Ratatotok Ratahan and Tombatu). The study used purposive sampling method at altitude 0-300 m asl, 301-600 m above sea level and> 601 m asl. The results showed that the presence of pests Paraeucosmetus sp. spread evenly by altitude in Southeast Minahasa regency with the intensity of attacks categorized as mild attacks. Keywords: Distribution of population, Paraeucosmetus sp.
Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti Makkiah, Makkiah; Salaki, Christina L; Assa, Berty
JURNAL BIOS LOGOS Vol 10, No 1 (2020): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.10.1.2020.27977

Abstract

Efektivitas Ekstrak Serai Wangi (Cimbopogon nardus L.) sebagai Larvasida Nyamuk Aedes aegypti (The Effectiveness of Citronella Extract (Cymbopogon nardus) as Larvaside of Aedes aegypti) Makkiah*), Christina L Salaki **), Berty AssaProgram Studi Entomologi Pascasarjana Universitas Sam RatulangiManado, 95155*Email: khiaimoet@gmail.com,**Email korespondensi: christinasalaki@ymail.com  (Article History: Received 20-10-2019; Revised 27-11-2019; Accepted 30-12-2019) AbstrakPengendalian nyamuk sebagai vektor utama penularan penyakit DBD hanya dapat dilakukan dengan cara memutuskan rantai penularannya melalui pengendalian nyamuk. Pengendalian nyamuk dapat dilakukan pada tahap larva dengan menggunakan biolarvasida yang terbuat dari bahan alami dan salah satunya adalah dengan menggunakan ekstrak kasar dari tanaman serai wangi (Cymbopogon nardus). Penelitian ini bertujuan untuk menguji efektivitas ekstrak serai wangi sebagai larvasida larva Aedes aegypti instar III dan IV. Penelitian ini adalah penelitian eksperimental menggunakan rancangan acak lengkap. Larva yang digunakan adalah larva instar III dan IV, dengan  perlakuan konsentrasi ekstrak serai wangi 20%, 30%, 40%, dan 50% dalam 250 ml air. Pada penelitian ini terdapat kontrol yaitu aquades yang tidak ditambahkan larutan apapun. Pengamatan kematian larva dilakukan pada jam ke 1, 3, 6, 12, 24, dan jam ke 48 dengan replikasi sebanyak 4 kali. Data presentase mortalitas larva digunakan untuk menghitung nilai LC50 dan LT50 dengan menggunakan analisisi Probit. Hasil penelitian menunjukkan ekstrak serai wangi efektif dalam mematikan 50% dari populasi  larva uji  dengan  nilai LC50 pada konsentrasi 36,48%serta waktu yang di butuhkan untuk mematikan  50% populasi larva uji adalah 10,45 jam. Kata kunci : Ekstrak, Cymbopogon nardus, larvasida, Aedes aegypti AbstractControl of mosquitoes as the main vector of transmission of DHF can only be done by breaking the chain of transmission through mosquito control. Mosquito control can be carried out at the larval stage by using biolarvasides made from natural materials, one of which is by using a crude extract from the citronella plant (Cymbopogon nardus). This research is to find out the effectiveness of citronella extract as larvae of Aedes aegypti larvae instar III and IV. This research is a pure experimental study using a complete random design. The larvae used were instar larvae III and IV, with the treatment of citronella extract concentration of 20%, 30%, 40%, and 50% in 250 ml of water. In this study, there was a control i.e. aquades which did not add any solution. Observation of larvae death was done at 1 hour, 2 hours. 4 hours, 12 hours, 24 hours and 28 hours with replication 4 times. Data on larval mortality were used to calculate LC50 and LT 50 values using Probit regression analysis. The results showed that citronella extract was effective in killing 50% of the population of test larvae with LC50 values at a concentration of 36.48% and the time needed to kill 50% of the population of test larvae was 10,450 hours.Keywords:  Extract, Cymbopogon nardus, larvasida, Aedes aegypti
Hubungan Tempat Perindukan dengan Kepadatan Larva Aedes aegypti sebagai Vektor Penyakit Demam Berdarah Dengue di Wilayah Kerja Puskesmas Kalumata Kota Ternate (Relationship of the Breeding Place with the Density of Aedes aegypti Larva as a Dengue Haemorhagic Fever Disease Vector in the Working Area of Kalumata Puskesmas Ternate City) Washliyah, Sitti; Tarore, Dantje; Salaki, Christina
JURNAL BIOS LOGOS Vol 9, No 2 (2019): JURNAL BIOS LOGOS
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jbl.9.2.2019.24174

Abstract

Hubungan Tempat Perindukan dengan Kepadatan Larva Aedes aegypti sebagai Vektor Penyakit Demam Berdarah Dengue di Wilayah Kerja Puskesmas Kalumata Kota Ternate (Relationship of the Breeding Place with the Density of Aedes aegypti Larva as a Dengue Haemorhagic Fever Disease Vector in the Working Area of Kalumata Puskesmas Ternate City) Sitti Washliyah¹*), Dantje Tarore1), Christina Salaki1**)¹)Program Studi Entomologi Pascasarjana Universitas Sam Ratulangi Manado, 95115*Email:lilihoeman@gmail.com**Email korespondensi: christinasalaki@ymail.com Diterima 2 Juli 2019, diterima untuk dipublikasi 5 Agustus 2019 Abstrak Demam berdarah dengue merupakan salah satu penyakit yang disebabkan oleh nyamuk Aedes aegypti dan Aedes albopictus sebagai vektor primer. Pengendalian tempat perindukan nyamuk Ae. aegypti lebih banyak dititikberatkan pada penutupan dan abatisasi bak mandi serta penguburan barang-barang bekas di sekitar rumah penduduk yang berpeluang sebagai penampung air hujan, sementara penampung air lainnya belum mendapat perhatian yang lebih memadai, padahal peluang untuk dijadikan sebagai habitat Ae. aegypti cukup besar. Tujuan penelitian ini yaitu untuk menganalisis hubungan tempat perindukan dengan kepadatan larva dan membandingkan kepadatan populasi larva pada setiap tempat perindukan nyamuk Ae. aegypti. Jenis penelitian kuantitatif dengan pendekatan cross sectional dan analisa uji chi square yang dilaksanakan di wilayah kerja Puskesmas Kalumata Kota Ternate. Hasil penelitian menunjukan bahwa ada hubungan antara tempat perindukan nyamuk dengan kepadatan larva berdasarkan karakteristik jenis, warna, bahan, letak dan kondisi. Kesimpulannya yaitu terdapat hubungan antara tempat perindukan dengan kepadatan larva Ae.aegypti sebagai vektor penyakit demam berdarah dengue.Kata kunci: Identifikasi, tempat penampungan air, survey jentik.  AbstractDengue hemorrhagic fever is a disease caused by mosquitoes species Aedes aegypti and Aedes albopictus as primary vectors. Control of breeding sites for Ae mosquitoes. aegypti is more focused on the closure and abatement of bathtubs and the burial of used goods around people's homes that have the opportunity to collect rainwater, while other water reservoirs have not received more adequate attention, even though the opportunity to be used as Ae habitat. aegypti is quite large. The purpose of this study was to determine the relationship between breeding sites with larval density and compare larval population densities at each breeding site of the Ae mosquito. aegypti. Type of quantitative research method with cross sectional approach and analysis of chi square test conducted in the working area of Kalumata Health Center, Ternate City. The results showed that there was a relationship between mosquito breeding sites and larval density based on the characteristics of the species, color, material, location and condition. The conclusion is that there is a relationship between breeding sites and the density of Ae.aegypti larvae as a vector of dengue hemorrhagic fever.Keywords: Identification, water reservoir, larva survey.
Aplikasi Metode Sidikjari Protein (SDS-PAGE) untuk Identifikasi Isolat Bakteri Endogenik Indonesia (Bacillus thuringiensis Berliner) yang Patogenik terhadap Hama Kubis (Crocidolomia binotalis Zell) Christina L. Salaki; Langkah Sembiring; Jesmandt Situmorang; Niken Satut Nur Handayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 17, No 2 (2012): June 2012
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v17i2.135

Abstract

Isolat bakteri Bacillus thuringiensis endogenik Indonesia yang patogenik terhadap hama kubis (Crocidolomia binotalis Zell.) dikarakterisasi dan diidentifikasi secara kimiawi dengan metode sidikjari protein. Total protein selular terlarut 10 isolat terpilih (SLK2.3, SRNG4.2, TKO1, TK9, YPPA1, UG1A,BLPPN8.2, YWKA1, BAU3.2, LPST1) dan 2 strain acuan (B. thuringiensis serovar kurstaki ATCC 10792 dan B. thuringiensis serovar israelensis H14) diperoleh melalui pemecahan sel dengan sonikasi. Ekstrak sel disentrifugasi dengan kecepatan 13000 rpm selama 5 menit untuk memperoleh supernatant. Protein dipisahkan dengan metode elektroforesis (SDS-PAGE) untuk menghasilkan profil sidikjari protein yang didokumentasikan dalam bentuk file elektronik. Selanjutnya, sidikjari protein dianalisis secara kualitatif maupun secara kuantitatif menggunakan software MVSP (Multivariate Statistical Package) untuk menghasilkan dendrogram. Hasil penelitian menunjukkan bahwa profil protein yang dihasilkan oleh tiap-tiap isolat dan strain acuan memberikan pola yang bermakna sehingga dapat digunakan untuk mengkarakterisasi dan mengidentifikasi isolat secara tegas. Analisis terhadap dendrogram menunjukkan bahwa strain acuan B. thuringiensis serovar kurstaki HD1 dapat dibedakan secara tegas dan jelas dengan strain acuan B. thuringiensis serovar israelensis H14. Dengan demikian, dapat disimpulkan bahwa aplikasi profil sidikjari protein merupakan instrumen yang sangat ampuh untuk menyingkap keanekaragaman strain anggota B. thuringiensis yang disolasi dari habitat alami. Isolat yang diteliti diidentifikasi sebagai strain baru anggota B. thuringiensis serovar kurstaki karena menunjukkan kemiripan yang tinggi dengan strain acuan B. thuringiensis serovar kurstaki ATCC 10792 meskipun ke-10 isolat ini juga menunjukkan keanekaragaman genetik yang cukup tinggi.
Uji Patogenisitas Isolat Bakteri Indigenous (Bacillus thuringiensis) terhadap Serangga Hama Kubis (Crocidolomia binotalis Zell) Christina L. Salaki; Jesmandt Situmorang; Langkah Sembiring; Niken Handayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v14i3.2582

Abstract

Pathogenicity of 34 indigenous B. thuringiensis isolates against C. binotalis were determined. The pathogenicity test was conducted by using leaf dipped method with various spore concentrations. Third instar larvae of C. binotalis were used as insect test. Mortality data of test larvae were used to determine the pathogenicity of the isolates in terms of 72 hours LC50 by using probit analysis. The results of experiments showed YPPA 1. was the most pathogenic isolate, producing 72 hours LC50 = 9.5 x 103 spore.ml-1 with LT50 (1.5 x 107 spore.ml-1) of 24.6 hours while the ACH 2.3 was found to be the least pathogenic isolate with 72 hours LC50 = 2.3 x 106 spore.ml-1 and LT50 (1.5 x 107 spoore.ml-1) of 40.7 hours. The shortest LT50 (1.5 x 107 spore.ml-1 was found to be 18.2 hours produced by TUS.1 with 72 hours LC50 = 3.9 x 105 spore.ml-1 whereas the longest LT50 (1.5 x 107 spore.ml-1) was found tobe 83.2 hours produced by the SLK 4.1 with 72 hours LC50 = 3.1 x 104 spore.ml-1. Therefore, it can be concluded that both YPPA.1 and TUS.1 isolates are potential candidate to be developed for biological control agent.
Analisis Keanekaragaman Isolat Bacillus thuringiensis yang Patogenik terhadap Serangga Hama Kubis (Crocidolomia binotalis) dengan Pendekatan Sistematika Numerik Christina L. Salaki; Jesmandt Situmorang; Langkah Sembiring; Niken Handayani
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v15i3.2605

Abstract

Diversity of B. thuringiensis (Bt.) isolates pathogenic to C. binotalis was determined by using Numerical Systematic Method. Ten isolates were taken to represent 34 pathogenic isolates along with two reference strains namely B. thuringiensis serovar kurstaki and B. thuringiensis serovar israelensis. The test isolates were examined for 89 phenotypic characters by using convensional method for colonial and cell morphology (37 characters) as well as physiological characteristics (3 characters) but biochemical characterization (49 characters) was conducted by using commercial API-50 CHB procedures. All phenotypic characters existed in one of two mutually exclusive states and were either scored plus (1) of minus (0). The binary data were prepared in Programmer’s File Editor (PFE) software. The data then were analysed by using the Multi Variate statistical Package (MVSP) Plus-Version 3.1 using the Simple Matching Coefficient (SSM). Clustering was achieved using the UPGMA algorithm. The results were presented as dendrograms. It was obtained that the test isolates were clearly assigned to two distinct multimembered clusters defined by 79.6 similarity level (S-level) in the SSM, UPGMA analysis. The two distinct clusters represented by each of two widely known different group of Bt. strains, namely serovar israelensis and serovar kurstaki. The first cluster contained reference strain of B. thuringiensis serovar israelensis, and two of the isolates (Slk2.3, and YPPA1) and the second cluster contained another reference strain of B. thuringiensis serovar kurstaki, and 8 of the isolates. Therefore, it strongly suggested that the application of numerical-fenetic analysis could provide a tool to unravel the strain diversity belong to B. thuringiensis.
IbM Pengendalian Hama Terpadu (PHT) pada Tanaman Sayuran di Kota Tomohon Christina L. Salaki; Sherlij Dumalang
Jurnal Pengabdian kepada Masyarakat (Indonesian Journal of Community Engagement) Vol 2, No 2 (2017): Maret
Publisher : Direktorat Pengabdian kepada Masyarakat Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (360.957 KB) | DOI: 10.22146/jpkm.27281

Abstract

An integrated pest control on vegetables crops in the fields,  is one of the control method to suppress the insect pest population , therefore farmer do not depend on the chemical controls that have negative effect both on the environment and human as well as animal.  The science and technology program for communities [IbM] aim to get a package of integrated pest control technology that is enviromentally friendly and it is derived from Indonesian microbial resources to support sustainable agriculture system .  To achieve  the goal, the program  implemented  through counseling, plot demonstration and field work.  These activities provided motivation  to farmers to be able to use biological agents combined with culture control to suppress population of insect pest and vegetables through the extension activities and the plot  demonstrations.  The IbM activity gave benefit to farmers by transfer knowledge through extension methods on how to get and use biopesticides with its theory and it can be directly practiced by farmers in the form of a plot demonstrations.
Aplikasi Pestisida Organik untuk Pengendalian Hama Spodoptera frugiperda pada Tanaman Jagung Christina Leta Salaki; Jackson Watung
Seminar Nasional Lahan Suboptimal 2020: Prosiding Seminar Nasional Lahan Suboptimal ke-8 “Komoditas Sumber Pangan untuk Meningkatkan K
Publisher : Pusat Unggulan Riset Pengembangan Lahan Suboptimal (PUR-PLSO) Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Salaki CL, Watung J. Application of  organic pesticides for pest control spodoptera frugiperda in corn plants. In: Herlinda S et al. (Eds.), Prosiding Seminar Nasional Lahan Suboptimal ke-8 Tahun 2020. Palembang 20 Oktober 2020. pp. xx. Palembang: Penerbit & Percetakan Universitas Sriwijaya (UNSRI).Spodoptera frugiperda is a polyphagous insect pest that can cause significant yield loss if not handled properly. Not many specific control technologies to control HSMS have been found. This study aims to obtain an organic pesticide formulation to control S. frugiperda. This study used 5 organic pesticide treatments (lemon grass, clove leaves and cinnamon) at concentrations of 10%, 25%, 40%, 55% and 70%) and Control. Pesticide application is carried out by three methods, namely: Insect Spray Method, Plant Spray Method and Insect Spray Method on Plants. The parameters observed included Symptoms of Damage, Percentage of Mortality and Time of Death. Larval mortality was observed at 12,24, 48 and 72 hours after application. The difference in the proportion of mortality between levels of concentration and control was tested using the Chi-Square method, while the LT50 was tested using probit analysis. The results showed that for the insect spray method of 5 treatments the concentration of Citronella could cause 13.3-67.7% mortality, 6.7-50.0% plant spray method, insect spray method on plants 23.3-86.7 % at 72 hours after application. Meanwhile, the use of clove leaves, with 36.7-76.7% Insect Spray Method, 16.7-56.7% Plant Spray Method, 50.0-93.3% Insect Spray Method on Plants. Whereas in the use of Cinnamon, the Insect Spray Method 33.3- * 6.7%, the Plant Spray Method 20.0-60.0% and the Insect Spray Method on Plants 46.7-100% at 72 hours after application. Time of death (LT50) at a concentration of 70% treatment with the insect spray method on plants within 12 hours after application and the longest at a concentration of 10% by the plant spray method.
Efektivitas Biolarvasida Serai Wangi (Cymbopogon nardus) Dengan Daun Cengkeh (Syzygium aromaticum L.) Terhadap Larva Aedes aegypti Christina L Salaki; Herlina Wungouw; Henny V Makal
JURNAL ILMIAH SAINS Volume 21 Nomor 2, Oktober 2021
Publisher : Sam Ratulangi University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (495.811 KB) | DOI: 10.35799/jis.v21i2.35616

Abstract

Penelitian ini menguji efektivitas kombinasi biolarvasida minyak atsiri serai wangi (Cymbopogon nardus) dengan daun cengkeh (Syzygium aromaticum l.) Terhadap larva nyamuk Ae. aegypti. Penelitian ini bertujuan untuk: (a) menganalisis pengaruh pemberian kombinasi biolarvasida minyak atsiri serai wangi dan daun cengkeh terhadap perkembangan larva Ae. aegypti, (b) menganalisis uji daya bunuh kombinasi biolarvasida serai wangi (sw) dan daun cengkeh (dc) terhadap larva Ae. aegypti, (c) menganalisis uji patogenisitas dari kombinasi biolarvasida serai wangi dan daun cengkeh terhadap larva Ae. aegypti. Uji kombinasi biolarvasida ini dilakukan terhadap larva nyamuk Ae. aegypti instar III  dengan perlakuan variasi kombinasi konsentrasi “SW 2% (P)”, “ SW 0,75% ” + “DC1,25% (P1)”, “SW 1%” + “DC 1% (P2)”, “SW 1,25 %” + “DC 0,75% (P3)” , “DC 2% (P4)”. dan Kontrol air (P-). Themepos (P+). Parameter yang diamati meliputi gejala, persentase mortalitas dan waktu kematian.  Mortalitas larva diamati pada jam ke-6,12,18,24  setelah aplikasi. Perbedaan proporsi mortalitas antar tingkat konsentrasi dengan control diuji dengan metode chi-kuadrat sedangkan lT50 dengan analisis probit.  Hasil penelitian menunjukkan masing-masing kombinasi bahan uji (P,P1,P2,P3 dan P4) tidak memiliki perbedaan pengaruh yang sginifikan sebagai biolarvasida dengan taraf signifikansi sebesar 0,05.. Kesimpulan hasil penelitian ini bahwa kelima konsentrasi bahan uji tersebut tidak memberikan perbedaan pengaruh yang signifikan sebagai biolarvasida. Hal ini menunjukkan bahwa minyak atsiri serai wangi dan daun cengkeh baik diaplikasi secara tunggal maupun dikombinasi memiliki efektifitas larvasida yang sama dengan temephos dalam membunuh larva nyamuk Ae. aegypti. Waktu kematian (LT50)  ekstrak serai wangi 12,72 jam, daun cengkeh 6,71 jam dan kombinasi 6,38 jam.Kata kunci: Biolarvasida; efektifitas; konsentrasi; patogenisitas The Effectiveness of Biolarvacide Essential Oil of Vitronella (Cymbopogon nardus) with Clove Leaves (Syzygium aromaticum.L.) on Ae. aegypti Mosquito Larvae ABSTRACTThis study tested the effectiveness of the biolarvicide combination of citronella essential oil (cymbopogon nardus) and clove leaf (syzygium aromaticum L.) against the larvae of Ae. aegypti. This study aims to: (a) analyze the effect of giving a combination of biolarvicides of citronella essential oil and clove leaves on the development of Ae. aegypti, (b) analyzing the killing power of the combination of citronella (sw) and clove leaf (dc) biolarvicides against Ae. aegypti, (c) analyzing the pathogenicity test of the combination of citronella and clove leaf biolarvicides against the larvae of Ae. Agypti. This biolarvicide combination test was carried out on the larvae of Ae. aegypti instar iii with treatment variations in the concentration combination “SW 2% (P)”, “SW 0.75%” + “DC1.25% (P1)”, “SW 1%” + “DC 1% (P2)”, “SW 1.25%” + “DC 0.75% (P3)” , “DC 2% (P4)”. and Water control (P-). Themepos (P+). Parameters observed included symptoms of mortality percentage and time of death. Larval mortality was observed at 6, 12, 18, 24 hours after application. The difference in the proportion of mortality between concentration levels and control was tested by the chi-square method while the lt50 method was tested by probit analysis. The results showed that each combination of test materials did not have a significant difference in effect as a biolarvicide with a significance level of 0.05. The conclusion of this study was that the five concentrations of the test material did not give a significant difference in effect as a biolarvicide. This indicates that the essential oils of citronella and clove leaves, either applied singly or in combination, have the same larvicidal effectiveness as temephos in killing the larvae of Ae. aegypti. Time of death (lt50) citronella extract 12.72 hours, clove leaf 6.71 hours and the combination 6.38 hours.Keywords: Biolarvicides; effectiveness; concentration; pathogenicity
Co-Authors Anggreini Kilala Anggreini Kilala, Anggreini Ariyane O.S. Siwu Assa, Berty Bastian P. Wowiling Bastian P. Wowiling, Bastian P. Betsy A.N. Pinaria Carla F. Kairupan Caroulus S. Rante Christian F.A. Rumende Christian L. Kaparang Daisy S. Kandowangko Dantje T Sembel Dantje Tarore Elisabet R.M. Meray Emmy Senewe Erwin G Kristanto Evie Paendong Gladys M. Tumanduk Guntur S.J. Manengkey Hans Huijbregts Henny Makal Henny Makal, Henny Henny V Makal Henny V.G. Makal Herlina I. S. Wungouw Herlina Wungouw Inri Wowor Ir. Caroulus S. Rante, MS Jackson Fraky Watung Jackson Watung Jackson Watung James B. Kaligis Jantje H. Manangka Jantje Pelealu Jantje Pelealu Jaya, Hendra K. Jesmandt Situmorang Jesmandt Situmorang Jimmy Rimbing Juliet E.M. Mamahit Juliet M.E. Mamahit Juliet M.E. Mamahit Krisen, Jeane Krisen Kumolontang, Geyby ., Langkah Sembiring LANGKAH SEMBIRING Makal, Henny V Makal, Henny V. Makkiah, Makkiah Manginstar, Christian O. Max ., Tulung Max Tulung Montong, Vivi B Montong, Vivi B. Moulwy F. Dien Natalia Samba Niken Handayani Niken Handayani Niken Satut Nur Handayani Noni N. Wanta Nurdjannah J. Niode Oddi R Pinontoan Odi R. Pinontoan Rafel S. Saroinsong Redsway Maramis Rivo Manopo Rondonuwu, Frans. B. Sammy S.N. Rori Sherlij Dumalang Threis Umboh Trina E. Tallei, Trina E. Tuldenigsri H. Gigir Tulung, Max ., Vera E. Tangkilisan Washliyah, Sitti Waywind Soewarno Yukiko Susandi Zainudin Limbanadi