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Journal : IPTEK The Journal for Technology and Science

Fiber Optic Adaptive Probe as a Cuvette Substitution for UV-Vis Spectrophotometer (Genesis 10S) Kurniawan, Fredy; Suharmanto, Edi
IPTEK The Journal for Technology and Science Vol 25, No 2 (2014)
Publisher : IPTEK, LPPM, Institut Teknologi Sepuluh Nopember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.12962/j20882033.v25i2.536

Abstract

Fiber optic adaptive probe UV-Vis for Genesis 10S has been fabricated to substitute cuvette for real time analysis. Most of standard spectrophotometers UV-Vis (the common type) use cuvette to place sample which is being analyzed. The light pass through the sample in the cuvette to detector then the intensity of the absorption of the solution is measured. Most of the cuvette system requires to transferring sample solution to cuvette from its original vessel, so the real time analysis cannot be conducted. The probe with special design using fiber optic cable FT-Au tonics 420-10 has been made to substitute the cuvette system. The light from the source moved inside fiber optic cable, which has been cut in the middle of the cable (the sample can passes through at this gap), to the detector. The end (entrance and exit) of the fiber optic was designed so that it fit to the compartment of the Genesis 10S spectrophotometer without any modification from the standard instrument condition. The spectrum results from the probe in varies gap, in the range 0.2 – 1 cm, was observed. The gap in the middle of the fiber optic was equal to the length of the pathway in the cuvette system. Rhoda mine B was used as the sample with variation concentration 4.175 × 10-5 M, 3.34 × 10-5 M, 2.5 × 10-5 M, and 1.67 × 10-5 M. The spectrum result of adaptive fiber optic probe was compared to the spectrum of standard cuvette system. The optimum result was provided using 0.2 cm gap. The maximum wavelength absorbance of adaptive fiber optic probe was at 533 nm which similar to the cuvette system
Co-Authors ., Syukrianto ., Umarudin Abdullah Aman Damai Adi Prawito Adi Soeprijanto Alvin Juniawan Anang Maryanto Anggriawan, Wahyu Ardhelia Maura Felisia Ari Nugroho Arif Rachmat, Arif Arynta Dharmayanti, Arynta Atik Rohmana Maftuhatul Fuad Baginda Zulkarnain Bagus, Muhammad Bahrul Ulum Dennis Farina Nury Diah Ayu Restuti Wulandari Diah Susanti Dipta Nusbangga, Dipta Djarot Sugiarso Endang Tri Wahyuni Endang Tri Wahyuni Erna Rochana Estu Yoga Elmi Gesa Febri Hadi Fitriyana Fitriyana Fransisca Bangun, Yulia Frizka Vietanti Fuji Ridha, Fuji Galuh Tiara Dewi HANGGARA, ANGGY YOGA Hariyati Purwaningsih Harmami Harmami Harmami Harmami Harus Laksana Guntur Hatta, Agus Muhamad Hendro Juwono Hendro Juwono Hendro Juwono Hendro Sutowijoyo Ika Puspita, Ika Ikhsan Setiawan Imam Abadi Isa, Illyas Md Ismail Azizi Ita Ulfin Ita Ulfin Julistyana Tistogondo Karlinda Karlinda Kartika A. Madurani Kaskoyo, Hari Koespiadi Kolo, Maria Magdalena Lia Marisca Permatasari Lourentia Candle Lutfi Andre Yahya Madurani, Kartika Anoraga Mahendra Wardhana, Mahendra Mega Vania Misbah Misbah Mochammad Zaki Nasrulloh Mohammad Teguh Hermanto Muhammad Alwi Syahara, Muhammad Alwi Muhammad Rivai Ni'mah, Yatim NUR EFENDI Nyoman Agus Sandika Purnaman Palupi Irene Desilvia Pemal Kabogi Praditya Bagaskara Qonitah Fardiyah R. Djarot Rahadian Zainul Rahmi Rahmi Rajwa Abidah Sudjarwo Ramadhana Sangyang Ady Regina Tutik Padmaningrum Rika Wijiyanti, Rika Romadhona, Syahrur Rooslan Edy Santosa Safira Aulia Sayyida Sayyida Sekartedjo Koentjoro, Sekartedjo Selvi Marcellia Setyadi Laksono Bahar Silvya Anggraini Sri Wiwoho Mudjanarko, Sri Wiwoho Suharmanto, Edi Sunarto Sunarto Suprapto Suprapto Suprapto Suprapto Suprapto, Suprapto Sutarno Sutarno Sutarno Sutarno Suyanta Suyanta Syahputra, Muhammad Yudha Syukrianto, Syukrianto Taslim Ersam Tri Paus Hasiholan Hutapea, Tri Paus Hasiholan Tri Widjaja Triyono Triyono Triyono Triyono Umarudin, Umarudin Vietanti, Frizka Wahyu Anggriawan Yunia Anisa Putri Yunita Triana Yunita Triana Zeni Rahmawati Zulkarnain Zulkarnain