Claim Missing Document
Check
Articles

Pengaruh Konsentrasi Kolkisin Terhadap Fenotipe Tanaman Air Mata Pengantin (Antigonon leptopus) Muhammad Akhlishil Ishlah; Muhammad Akhlish; Prameswari Permata Insani; Florentina Kusmiyati
JAGROS : Jurnal Agroteknologi dan Sains (Journal of Agrotechnology Science) Vol 7, No 1 (2022): JAGROS : Jurnal Agroteknologi dan Sains (Journal of Agrotechnology Science)
Publisher : Universitas Garut

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52434/jagros.v7i1.2113

Abstract

Antigonon leptopus is one of the flower plants that honey bees like because they contain nectar and pollen. The purpose of this study was to examine the effect of colchicine concentration on the phenotype of Antigonon leptopus. This study used a monofactor completely randomized design (CRD) with 7 replications. The treatment factors were 5 levels of colchicine concentration consisting of K0: 0 % (control), K1: 0,1 %, K2: 0,2 %, K3: 0,3 %, and K4: 0,4 %. The research data analysis used the F test and if it had a significant effect, it was continued with the DMRT test with level of α = 5 %. The results showed that soaking the seeds for 12 hours with colchicine was able to increase the diversity of phenotypes of Antigonon leptopus. The 0,1 % colchicine treatment had the best diversity in the parameters of tendril length, number of leaves, leaf area, number of flowers, flower diameter, flowering age and pollen weight, namely 76,8 cm, 34,6 cm, 61,5 cm2, 61,6 flowers, 3,0 cm, 44,4 days after planting, and 0,045 g. The 0,1 % colchicine treatment also showed a more concentrated flower color than the control. Colchicine treatment had no significant effect on the anther number parameter.
Respon Bibit Tanaman Pisang Raja Bulu (Musa paradisiaca L.) Tahap Aklimatisasi terhadap Berbagai Level Lama Penyinaran dan Penambahan Kombinasi Zat Pengatur Tumbuh Hakim Adil; Florentina Kusmiyati; Syaiful Anwar
Jurnal AGROHITA: Jurnal Agroteknologi Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan Vol 8, No 4 (2023): JURNAL AGROHITA
Publisher : Fakultas Pertanian Universitas Muhammadiyah Tapanuli Selatan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31604/jap.v8i4.14014

Abstract

Pisang Raja Bulu (Musa paradisiaca L.) merupakan salah satu tumbuhan tropis komoditas hortikultura buah dari family Musaceae. Perbanyakan tanaman pisang dilakukan secara vegetatif menggunakan tunas. Metode tersebut dinilai kurang efektif karena jumlah tunas yang dihasilkan terbatas. Perbanyakan melalui kultur in vitro merupakan salah satu upaya untuk memproduksi bibit berkualitas dalam jumlah yang besar dan dalam jangka waktu yang relatif lebih singkat. Kekurangan dari kultur in vitro yaitu memiliki persentase keberhasilan yang kurang pada tahap aklimatisasi. Pengaturan lama penyinaran dan penambahan zat pengatur tumbuh giberelin diharapkan dapat menunjang pertumbuhan bibit dan meningkatkan persentase keberhasilan aklimatisasi. Penelitian ini telah dilaksanakan pada 2 Mei 2022 – 11 Juli 2022 di sepetak lahan di Kecamatan Boja, Kabupaten Kendal, Jawa Tengah. Penelitian ini menggunakan rancangan split plot 4 x 3 dengan dasar Rancangan Acak Kelompok (RAK) diulang 5 kali. Petak utama adalah lama penyinaran dengan 4 taraf yaitu lama penyinaran 10 jam, 12 jam, 14 jam, dan 16 jam. Anak petak adalah penambahan hormon giberelin dengan 3 taraf konsentrasi yaitu penambahan giberelin 0 ppm, 50 ppm, dan 100 ppm. Parameter yang diamati yaitu tinggi tanaman pisang, diameter batang semu, jumlah daun, panjang daun, lebar daun, dan persentase keberhasilan aklimatisasi. Data tinggi tanaman, diameter batang semu, jumlah daun, panjang daun, dan lebar daun dianalisis kuantitatif dengan Analysis of Variance (ANOVA), selanjutnya perlakuan yang berpengaruh diuji lanjut dengan Duncan’s Multiple Range Test (DMRT) dan Polinomial Ortogonal (PO) pada taraf 5% untuk menentukan hasil perlakuan terbaik diantara semua perlakuan. Berdasarkan penelitian yang telah dilakukan, dapat disimpulkan bahwa lama penyinaran 16 jam dan penambahan giberelin 100 ppm efisien meningkatkan tinggi tanaman, diameter batang semu, jumlah daun, panjang daun, dan lebar daun. Lama penyinaran 10 jam dengan penambahan giberelin 50 ppm sudah dapat meningkatkan nilai panjang daun tanaman. Konsentrasi giberelin yang ditambahkan masih dapat ditingkatkan untuk mendapatkan lebar daun yang optimal. Persentasi keberhasilan aklimatisasi yang didapatkan yaitu 100%. 
Pengaruh Aplikasi Cendawan Mikoriza Arbuskular dan Pemupukan NPK Majemuk Dalam Pertumbuhan dan Produksi Tanaman Tomat Dwitomo, Antonius Bagas; Kristanto, Budi Adi; Kusmiyati, Florentina
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3573

Abstract

Kebutuhan buah tomat di Indonesia selalu meningkat sehingga perlu adanya tindakan peningkatan produksi dengan pemanfaatan mikroorganisme tanah dan pemupukan. Cendawan mikoriza arbuskular merupakan salah satu mikroorganisme tanah yang mampu meningkatkan penyerapan unsur hara dalam tanah sehingga memenuhi kebutuhan tanaman. Penelitian ini bertujuan untuk mengevaluasi dosis aplikasi inokulum cendawan mikoriza arbuskular (CMA) dan pemupukan yang terbaik untuk pertumbuhan dan produksi tanaman tomat. Penelitian dilaksanakan pada bulan Maret 2022 sampai dengan bulan Juni 2022 di Green House, Laboratorium Fisiologi dan Pemuliaan Tanaman, Laboratorium Ekologi dan Produksi Tanaman, Fakultas Peternakan dan Pertanian, Universitas Diponegoro, Semarang. Penelitian menggunakan Rancangan Acak lengkap (RAL) Faktorial 3 x 5 dengan 3 kali ulangan. Faktor pertama adalah perbedaan dosis CMA, yaitu J0: 0 g/tanaman, J1: 10 g/tanaman, J2: 20 g/tanaman. Faktor kedua adalah perbedaan dosis pemupukan yaitu, P1 = 60 kg/ha N, P2O5, dan K2O (400 kg/ha), P2 = 90 kg/ha N, P2O5, dan K2O (600 kg/ha), P3 = 120 kg/ha N, P2O5, dan K2O (800 kg/ha), P4 = 150 kg/ha N, P2O5, dan K2O (1000 kg/ha), P5 = 180 kg/ha N, P2O5, dan K2O (1200 kg/ha). Data dianalisis menggunakan ANOVA (Analysis of Variance) dan uji lanjut dengan Duncan Multiple Range Test (DMRT) pada taraf lima persen (5%). Hasil penelitian menunjukkan bahwa perlakuan dosis inokulum cendawan mikoriza arbuskular memberikan pengaruh nyata pada parameter persentase kolonisasi cendawan mikoriza dan jumlah daun tanaman tomat.
Pengaruh Pemberian Pupuk Kalium dan Giberelin terhadap Pertumbuhan dan Produksi Tanaman Cabai (Capsicum annum L.) Deviyanti, Vania Mulya; Kristanto, Budi Adi; Kusmiyati, Florentina
JURNAL AGROPLASMA Vol 10, No 1 (2023): JURNAL AGROPLASMA VOLUME 10 NO 1 TAHUN 2023
Publisher : UNIVERSITAS LABUHANBATU

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/agroplasma.v10i1.3571

Abstract

The need for chili in Indonesia is always increasing every year, so it is necessary to take action to increase production by fertilization and using plant regulatory substances. This study aims to examine the effect of giving the best dose of potassium fertilizer and gibberellin concentration for the growth and production of chili plants. The research was conducted from May 2022 to August 2022 at the Screen House, Laboratory of Physiology and Plant Breeding, Laboratory of Ecology and Plant Production, Faculty of Animal Husbandry and Agriculture, Diponegoro University, Semarang. The research design used a 4 x 4 factorial design on the basis of Completely Randomized Design (CRD) with 3 replications. The first factor was the difference dose of K2O, K1: control (without K2O), K2: 60 kg/ha K3: 120 kg/ha dan K4: 180 kg/ha. The second factor was the difference concentration of gibberellin, G1: control (0 ppm), G2: 100 ppm G3: 125 ppm dan G4: 150 ppm. The data was analyzed using ANOVA (Analysis of Variance) and further test with Duncan Multiple Range Test (DMRT) at the level of five percent (5%). The results showed that the treatment of K2O fertilizer had a significant effect on the parameters of the plant height, the number of leaves and potassium up take of chili plant. Keywords: chili,gibberellin, K2O
Pengelolaan DAS Mikro: Best Practice Penanganan Potensi Konflik Akibat Semburan Gas di Lahan Pertanian Desa Nglobo, Kabupaten Blora, Jawa Tengah Sriyana, Ignatius; Sangkawati, Sri; Roselyn, Melfina; Kusmiyati, Florentina; Ramadan, Bimastyaji Surya
MEDIA KOMUNIKASI TEKNIK SIPIL Volume 30, Nomor 1 (2024)
Publisher : Department of Civil Engineering, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/mkts.v30i1.60034

Abstract

Semburan gas di lahan pertanian Desa Nglobo, Kabupaten Blora, Indonesia, menyebabkan kontamisasi lahan, sehingga kesehatan Daerah Aliran Sungai (DAS) mengalami penurunan. Penurunan kesehatan DAS terlihat pada air dan tanah yang melebihi baku mutu Hal ini menyebabkan terjadinya konflik di masyarakat karena produktivitas tanaman padi dan jagung mengalami gagal panen. Penelitian ini bertujuan mengembalikan kesehatan daerah aliran sungai yang terkontaminasi baik lahan pertanian dan kualitas air. Metode yang digunakan adalah dengan pendekatan pengelolaan DAS mikro yang meliputi kegiatan berdiskusi dengan masyarakat dan para pihak lainnya,  usaha remediasi, sekolah lapangan dan monitoring evaluasi. Hasil kajian menunjukkan penambahan pupuk organik untuk mengembalikan kesuburan tanah terutama meningkatkan kandungan C-Organik sangat diperlukan yaitu dengan memberikan 29,58 ton/ha – 30,14 ton/ha pupuk pada lahan tersebut. Kualitas air setelah remediasi menunjukkan nilai di bawah ambang batas. Hasil evaluasi uji tanam menunjukkan padi dan jagung dalam kondisi baik, dibuktikan dengan tidak terjadinya kegagalan panen, kondisi kesuburan  lahan pertanian dapat pulih kembali. Pendekatan pengelolaan  DAS mikro, yang terukur, dengan aksi nyata  bersama masyarakat  terkena dampak dan para pihka yang terkait, dapat menyelesaikan konflik sosial yang terjadi akibat pencemaran pada lahan pertanian masyarakat.
Evaluasi Keragaman Mutan Generasi MV3 Aster Cina (Callistephus chinensis L.) Hasil Mutasi Induksi Sinar Gamma Chelsea, Fiorentina; Anwar, Syaiful; Sudarman, Adriani Darmawati; Kusmiyati, Florentina
Berkala Bioteknologi Vol. 3 No. 2 November 2020
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

ABSTRAK Aster cina (CallistephuschinensisL.) merupakansalahsatujenistanamanhias yang sering digunakan dalam industry bunga potong. Penelitian bertujuan untuk mengkaji keragaman genetik dan heritabilitas pada tanaman mutan aster cina MV3hasil mutasi radiasi sinar gamma. Iradiasi sinar gamma telah diaplikasikan pada anakan aster cina MV0dengan variasi dosis iradiasi Y0= 0 Gy, Y1= 5 Gy, Y2= 10 Gy, Y3= 15 Gydan Y4= 20 Gy. Bahan tanam MV3diambil dari generasi MV2 yang memiliki karakter berbunga cepat (46 anakan), jumlah bunga terbanyak (56 anakan) dan tangkai bunga terpanjang (59 anakan). Rancangan percobaan yang digunakan yaitu Rancangan Acak Kelompok non faktorial dengan 9 genotipe uji dan 1 genotipe kontrol sebagai pembanding. Hasil analisis ragam menunjukkan bahwa karakter umur muncul bakal bunga dan umur binga mekar sempurna tidak berbeda nyata terhadap pengaruh genotipe maupun pengaruh lingkungan. Karakter jumlah daun, jumlah anakan dan jumlah tangkai bunga memiliki heritabilitas sedang serta karakter jumlah bunga memiliki heritabilitas yang tinggi. Berdasarkan hasil penelitian dapat disimpulkan bahwa terdapat keragaman antara tanaman control dengan genotipe, sehingga dapat dilakukan evaluasi keragaman untuk generasi selanjutnya. Kata kunci:Callistephus chinensis L., genetik, keragaman, MV3,sinar gamma
Lilium longiflorum Plant Growth with a combination of Naphthylacetic Acid (NAA) and 6Benzylaminopurine (BAP) In Vitro Fauziah, Rosita Husnun; Kusmiyati, Florentina; Anwar, Syaiful
Journal of Tropical Crop Science and Technology Vol. 1 No. 2 (2019): VOLUME 1, NO. 2, OCTOBER 2019
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v1i2.10387

Abstract

This research was aim to identify the effect of Naphthylacetic Acid (NAA) and 6-Benzylaminopurin (BAP) on growth of Lilium longiflorum planlet. The research method was Completely Randomized Design Factorial 4 x 4 with 4 replication. First factor was concentration of NAA, consist of A0: 0 mg/l, A1: 0,75 mg/l, A2: 1,5 mg/l, and A3: 2,25 mg/l. Second factor was concentration of BAP, consist of : B0: 0 mg/l, B1: 0,3 mg/l, B2: 0,6 mg/l, and B3: 0,9 mg/l. The observed parameters were number of shoots, plant height, number of leaves, number of roots, and percentage of contamination. Data were process by Analysis of Variance and continued by Least Significance Different (LSD). The result showed that combination of NAA and BAP did not significantly affect the growth parameter of Lilium longiflorum. BAP treatment significantly affected the number of shoots and number of leaves. The number of roots only observed in NAA treatment; 0,3 mg/l BAP + 0,75 mg/l NAA; 0,3 mg/l BAP + 1,5 mg/l  NAA; and 0,6 mg/l BAP + 0,75 mg/l NAA. Percentage of fungal contamination was 7,5%. The conclusion of the result were the treatment 0f 0,3 mg/l BAP + 0,75 mg/l NAA was the best treatment for growth of Lilium longiflorum planlets with no contamination.
The Effect of Naftalene Acetic Acid and Benzyl Amino Purine on Growth of Potato Planlets In Vitro Nurmufiidah, Rahma; Kusmiyati, Florentina; Lukiwati, Dwi Retno
Journal of Tropical Crop Science and Technology Vol. 2 No. 1 (2020): VOLUME 2, NO. 1, MARET 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v2i1.10418

Abstract

The purpose of this research was to analyze the effect of Naftalene Acetic Acid (NAA) and Benzyl Amino Purine (BAP) on growth of potato plantlet (Solanum tuberosum L.). This research used factorial Completely Randomized Design (CRD). The first factor was the concentration levels of NAA (A1 = 0,5 ppm A2 = 1.5 ppm and A3 = 2.5 ppm). The second factor was the concentration levels of BAP (B1 = 1 ppm B2 = 2 ppm and B3 = 3 ppm). Parameter observed were number of nodes, number of branches, number of roots, percentage of life explants, and percentage of damaged explants. Data were analyzed with analysis of variance and continued by Least Significance Different (LSD). The results showed that NAA 0.5 ppm increased the number of nodes, branches, and  roots compared to other treatments. The concentration of BAP 1 ppm increased the number of roots compared to other treatments. The concentration of NAA 0.5 ppm and BAP 1 ppm showed the best percentage of life explants (100 %) and showed the minimum percentage of damaged explants (0 %).
Biological Agent’s Effectiveness for Control of Diamondback Moth Larvae (Plutella xylostella Linn) with Different Population Levels on Lettuce Mumtahanah, Zulfa Naila; Kristanto, Budi; Kusmiyati, Florentina
Journal of Tropical Crop Science and Technology Vol. 2 No. 2 (2020): VOLUME 2, NO. 2, OCTOBER 2020
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v2i2.10526

Abstract

Tritip caterpillars (Plutella xylostella) are pests that cause damage and reduce lettuce production. Control of P. xylostella pests can be done using biological agents or synthetic insecticides. Synthetic chemical pesticides in the long term can be used for resistance and resurgence of pests. Biological agents of B. bassiana, Metarhizium sp., and Trichoderma sp. can be used as an alternative solution to controlling P. xylostella. Research to examine the use of biological agents using B. bassiana, Metarhizium sp. and Trichoderma sp. to control P. xylostella attacks at different levels of pest participation. The study was conducted from 19 September to 9 November 2018 in Sidomukti, Bandungan, Semarang. The study used a completely randomized 5x3 split-plot pattern design with 3 replications. The main plot is the type of biological agents: A1: B. bassiana, A2: Metarhizium sp., A3: Trichoderma sp., A4: Insecticide and A5: control. The subplot is the pest population level: B1 = 1 larvae/plant, B2 = 2 larvae/plant, and B3 = 3 larvae/plant. Data were analyzed using ANOVA and continued with Duncan's Multiple Range Test. The results showed that biological agents and insecticides increased pest mortality, the percentage of healthy leaves, decreased the percentage of damaged leaves, the level of pest defense, and reduced the yield of lettuce.
Evaluation of Genetic Diversity and Heritability on Soybeans Agronomic Characters in M4 by Gamma Irradiation Sari, Yunita Mahda; Kusmiyati, Florentina; Anwar, Syaiful
Journal of Tropical Crop Science and Technology Vol. 3 No. 1 (2021): VOLUME 3, NO. 1, MARCH 2021
Publisher : Universitas Muhammadiyah Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22219/jtcst.v3i1.10538

Abstract

The production of soybeans can be increase by breeding for tolerance soybeans at saline soil. Mutation by gamma rays is one of a breeding technique that can be modifiy plant's genetic. The soybeans that used was induced by gamma rays with dosis 160 Gy, 208 Gy, 256 Gy, 352 Gy, and 400 Gy. The research objective was to evaluate the genetic variability and heritability for some characters of M4 soybean mutations resulting from gamma irradiation mutations.The material used consisted of 18 genotypes of M4. This research was conducted at Agrotechopark, Faculty of Animal and Agricultural Science, Diponegoro University from July to November 2019. The experimental was arranged in a completely randomized design (CDR) monofactor with 18 genotypes of black soybeans and Detam-3 Prida as controls. The results showed that genetic diversity observed in the overall character of plants except flowering age, number of empty pods, weight of one seed, and weight of 100 grains. The result of heritability showed a high heritability on the parameters of plant height, age of harvest, total number of seeds, number of filled pods, total number of pods, total pod weight, and total seed weight. The value of moderate heritability is found on the parameters of the number of leaves, and then the low heritability is on the parameters of flowering age, number of empty pods, weight of 1 seed, and weight of 100 grains.
Co-Authors A'yuni Fatkhi Fajriyati Afni Harfina Afni Harfina Akbar, Sifron Alya Hasna Irbah Septiani Amalia Wulannanda Andariza, Isna Panca Anggih Noor Alamsyah Anto Budiharjo Awang Ghosypea Azka, Millati B. Herwibawa Bagus Herwibawa Bakhtiar, Ikrima Farishani Budi Kristanto Chelsea, Fiorentina Civic Julian Pradewa Deviyanti, Vania Mulya Dharmasika, Intan Dwi Retno Lukiwati Dwi Sulastri Dwitomo, Antonius Bagas Elfira, Yolanda Eny Fuskhah Fauziah, Rosita Husnun Febryan Taufiq Fiona Fajarditta Firda Lailatus Sa’adah Hakim Adil Herwibawa, B. Holyness Nurdin Singadimedja Intan Dharmasika Ishlah, Muhammad Akhlishil K. Karno Karno Karno Khodijah Wafia Kristanto, Budi Adi Kristanto, Budi Adi Lika Alfariatna Lulu Fatikhatul Maryamah Maulana Azhar Adipraja Muhammad Akhlish Muhammad Akhlishil Ishlah Muhimmatul Ifadah Muhimmatul Ifadah Mumtahanah, Zulfa Naila Nabilatus Sunayya Nabilatus Sunayya Nanik Nurhana Natalia Indah Widyasmara Noor Fitriya Mirta Liana Nurhana, Nanik Nurmila Karimah Nurmufiidah, Rahma Nurul Anisa Nurul Fajriyah Nurul Shintawati Oktaviana Limbong pamungkas, wahyu widi kusuma Prameswari Permata Insani Purbajanti, Endang Purwoko, Agus Putra, Fajrin Pramana R. Djoko Soetrisno Rafaella C. Megananda Rafaella Chandraseta Megananda Rahmatika, Wardah Naila Ramadan, Bimastyaji Surya Riska Devita Aprianti Rizal Try Nofiyanto Roessali, Wiludjeng Rokhimun Tolib Roselyn, Melfina Rulia Ervina Dewi Sari Noor Hidayah Sari, Yunita Mahda Sembiring Depari, Muhammad Mukhlis Sifron Akbar Solichah, Urip Jamiati Sri Sangkawati Sachro Sriyana, Ignatius Sudarman, Adriani Darmawati Suharyani Suharyani Sumarsono Sumarsono Susilo Budiyanto suswati suswati Sutarno Sutarno Syaiful Anwar Velly Dontor Nahampun Widiyarso, Salsabila Mumtaz Widyati Slamet Yafizham, Yafizham Yanuar Rizqiani Zulfaa, Latifah