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Journal : THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST

Keanekaragaman Genetik Nyamuk Vektor Filariasis Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) di Kota dan Kabupaten Pekalongan Dengan Metode PCR-RAPD Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 2 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1087.745 KB) | DOI: 10.30651/jmlt.v1i2.1496

Abstract

Mosquito Culex quinquefasciatus is a vector of nematode worms, namely Wuchereria bancrofti which is the cause of filariasis in tropical and subtropical countries. Distributed of Cx. quinquefasciatus is widely in Indonesia with differences the geographical, resulting in the adaptation to the environment and may  results in a high variation, both phenotypic (morphology) and genotypic (genetic) variation. This study aims was to determine the genetic diversity of mosquitoes Cx.quinquefasciatus as vector filariasis in Pekalongan City and Regent. Genetic characterization performed by PCR-RAPD using three primers, ie OPA-11, OPA-12, and OPA-15. Data were analyzed by using UPGMA algorithm and Simple Matching Coefficient and presented as dendrogram. The results showed a high genetic diversity with the polymorphisms up to 100%.  Keywords: Culex quinquefasciatus, vector, filariasis, PCR-RAPD 
Gambaran Parasit Cacing Nematoda Usus Dan Cestoda Pada Feses Sapi (Boss sp.) Di Peternakan Sumber Jaya Ternak, Kecamatan Tikung, Kabupaten Lamongan, Jawa Timur Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 3, No 1 (2020): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (489.751 KB) | DOI: 10.30651/jmlt.v3i1.4632

Abstract

Cows are important animals for Indonesian farmers who have high economic value. Cow breeding business has many benefits for human life, especially meat, milk, bones, skin, offal and feces (cow dung). Cow feces can be used as an organic fertilizer manufacture by cattle farmers. But it needs to be considered in making organic fertilizer derived from cows because it contains many diseases and parasites. If the cow's feces are not treated properly, it will cause environmental pollution to the local residents. Cow infected with parasites can experience a decrease in body weight, decreased endurance, impaired growth and death. To find out intestinal helminth parasitic infections, one of them is by identifying worms in cow feces. The majority of the population of Tikung Subdistrict, Lamongan Regency, earn their living as farmers and ranchers. The purpose of this study was to identify intestinal Nematode worms in cow feces in Tikung Subdistrict, Lamongan Regency. The method used in this study was Nacl Saturated to determine the morphology and forms of parasites. The results showed that cattle feces samples taken from the Tikung Subdistrict of Lamongan District contained the Nematoda parasite, which was found the presence of Hookworm eggs in 3 samples with a percentage of 6% and found 1 positive sample (+) containing Taenia saginata eggs, with a percentage of 2% of the total 50 samples in Sumber Jaya Livestock Farm in cattle on Tikung Subdistrict, Lamongan Regency is still relatively low. Keywords: Cow Feces (Bos sp.), Intestinal Nematodes, Cestodes
Efektivitas Minyak Atsiri Serai Wangi (Combypogon nardus) Sebagai Insektisida Alami Untuk Kecoa Amerika (Periplaneta americana) Anindita Riesti Retno Arimurti
THE JOURNAL OF MUHAMMADIYAH MEDICAL LABORATORY TECHNOLOGIST Vol 1, No 1 (2018): The Journal of Muhammadiyah Medical Laboratory Technologist
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (193.116 KB) | DOI: 10.30651/jmlt.v1i1.1008

Abstract

  ABSTRAK Cockroaches are one of the most common types of insects around our neighborhood, and it is one of the most dangerous insects because some species of cockroaches are known to transmit disease in humans such as tuberculosis, typhoid, asthma, cholera, and hepatitis. Eradication of cockroaches can be done physically and chemically, the use of chemical-based insecticides can cause respiratory problems. In an insecticide drug containing natural ingredients contain essential oils, the content of essential oil compounds serve as an anti-insect drug on citronella. This study aimed to determine the effect of essential oils of citronella fragrance to cockroaches. This type of research was experimental. The population in this study were cockroaches (Periplaneta americana) obtained from the houses of villagers of Tanjung, Sampang, Madura. The total sample was 32 adult stage cockroaches. Data analysis was using free T test with error rate of  5% or 0,05.The results showed that cockroaches stopped moving with an average of 15.75. Based on T test results showed the significant results of 0.00 which was smaller than 0.05 then H0 was rejected Ha was accepted. It can be concluded that the essential oils of citronella fragrance have an effect on the death of cockroaches. Keywords: Cockroach, Essential Oil Of Citronella Fragrance (Cymbopogon nardus)