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Journal : Medicra (Journal of Medical Laboratory Science/Technology)

The Effect of Storage Time for Pooled Sera on Freezers on the Quality of Clinical Chemical Examination: Pengaruh Lama Penyimpanan Pooled Sera pada Freezer Terhadap Mutu Pemeriksaan Kimia Klinik Maulidiyanti, Ellies Tunjung Sari; Purwaningsih, Nur Vita; Widiyastuti, Rahma; Samsudin, Rinza Rahmawati; Arimurti, Anindita Riesti Retno
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 4 No. 2 (2021): December
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v4i2.1613

Abstract

A common problem in clinical laboratories is maintaining the stability of serum analytes during sample storage. Samples are usually stored in the door (4–8°C) of the refrigerator for a short period of time or in the freezer (-20°C) for a longer period of time (pre-analytical stage) and subsequently, during post-analysis sample handling (storage time and temperature). This study aims to analyze the stability in this case is the storage time of pooled sera in the freezer at temperatures up to -18º C and in the refrigerator at temperatures up to 4°C for 3 months with the inspection parameters, namely cholesterol and triglycerides. This type of research is experimental, with a research design that is time series control group design. The sample in this study was a control serum in the form of pooled sera stored in the freezer and refrigerator which was checked for cholesterol and triglyceride levels every 2 weeks for 3 months or for 12 weeks. The results showed that the average calculation of cholesterol levels in pooled sera stored in the freezer was 142.07 mg/dL and the average cholesterol level examination stored in the refrigerator was 161.89 mg/dL then the average the examination of triglyceride levels in pooled sera stored in the freezer is 112.89 mg/dL and the average examination of triglyceride levels stored in the refrigerator is 140.46 mg/dL. Based on the analysis of the research results, it can be concluded that, there is an effect of storage time in the freezer on the stability of cholesterol levels and triglyceride levels.
Prevalence Of Helmint Infection In Cat’s Feces In Surabaya City: Prevalensi Infeksi Helmin Pada Feses Kucing Di Kota Surabaya Rohmayani, Vella; Arimurti, Anindita Riesti Retno; Romadhon, Nurhidayatullah; Lihabi, Lihabi
Medicra (Journal of Medical Laboratory Science/Technology) Vol. 6 No. 1 (2023): July
Publisher : Universitas Muhammadiyah Sidoarjo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21070/medicra.v6i1.1685

Abstract

Cats are one of the most popular animals because of their cute and tame appearance. Just like other organism, cats can become infected worms if they are not kept clean. Cats infected with worms will initially lose their appetite, over time they will lose weight. Cats infected with worms, if kept and not treated continuously, can transmit to cat owners (humans). This study aims to determine the prevalence of parasitic worm infection in cat feces in the city of Surabaya, and to compare the prevalence of parasitic worm infection in domesticated cats with cats living wild in the city of Surabaya. The samples used in this study were 100 faecal samples, which were taken from 50 cats that live in the wild and from 50 live cats that are kept. Examination of faecal samples was carried out directly and indirectly using the saturated NaCl debris method, the data obtained will be presented descriptively and analyzed using the Chi-square test. Based on the results of the research conducted, the prevalence of worm infection in feral cat feces was 60%, while in domestic cats the prevalence was 10%. The types of worms that were found were Toxocara spp., Ancylostoma spp., Diphyllobothrium spp., and Trichuris spp. Based on the Chi-square test, it can be concluded that there is a significant relationship (P<0.05) between the prevalence of parasitic worm infection in domesticated cats and cats that live in the wild.