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All Journal Jurnal Ilmu dan Kesehatan Hewan Indonesia Medicus Veterinus Jurnal Ilmu Ternak Veteriner Sricommerce: Journal of Sriwijaya Community Services Buletin Veteriner Udayana
Hapsari Mahatmi
Laboratorium Bakteriologi Dan Mikologi Veteriner, Fakultas Kedokteran Hewan, Universitas Udayana, Jl. PB. Sudirman, Denpasar, Bali, Indonesia, 80234
Arts Humanities Economics, Econometrics & Finance Education Health Professions Immunology & microbiology Medicine & Pharmacology Public Health Social Sciences Veterinary

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Kontaminasi Salmonella spp pada Daging Ayam Broiler yang dijual di beberapa Pasar Tradisional di Makassar Darmawan, Alpian; Muslimin, Lucia; Arifah, Sitti; Mahatmi, Hapsari
Indonesia Medicus Veterinus Vol 9 (2) 2020
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (238.504 KB) | DOI: 10.19087/imv.2020.9.2.168

Abstract

Daging ayam merupakan bahan komoditi asal hewan yang pada beberapa tempat masih diperdagangkan dengan sistem konvensional, sesuai dengan kebiasaan masyarakat setempat. Kandungan protein dan kandungan air yang tinggi pada daging ayam merupakan media pertumbuhan bagi berbagai bakteri yang bersifat non patogen maupun yang patogen. Peraturan pemerintah menyatakan bahwa Salmonella spp merupakan salah satu bakteri patogen yang tidak boleh ada dalam daging ayam ataupun produk hewan lainnya. Penelitian ini bertujuan untuk mengetahui kemungkinan adanya kontaminasi Salmonella spp pada daging ayam yang dipasarkan di enam pasar tradisional di wilayah Makassar. Sampel yang diperiksa sebanyak 24 sampel berupa daging ayam bagian dada yang diambil dari enam lokasi pasar yang berbeda yaitu Pasar Daya (Kecamatan Biringkanaya), Pasar Antang (Kecamatan Manggala), Pasar Terong (Kecamatan Bontoala), Pasar Pabbaengbaeng (Kecamatan Tamalate), Pasar Sambung Jawa (Kecamatan Mariso), dan Pasar Bacan (Kecamatan Wajo) Kota Makassar. Isolasi dan identifikasi dilakukan dengan pemeriksaan mikroskopis dengan pewarnaan Gram dan dilanjutkan dengan isolasi pada media Bistmut Sulfit Agar (BSA), Salmonella Shigella Aga (SSA), uji TSIA (Triple Sugar Iron Agar), IMViC (Indole, Methyl Red, Voges Proskauer, Citrat) dan uji urease. Hasil penelitian ditemukan bahwa 3 (12,5%) isolat dari 24 sampel yang diisolasi dan diidentifikasi, positif bakteri Salmonella spp. Kondisi ini memerlukan perhatian serius untuk segera mendapatkan tindak lanjut oleh pemegang kebijakan terkait sebagai upaya pencegahan penyebaran penyakit zoonosis.
Bakteri Escherichia coli Teridentifikasi pada Rektum Lumba-Lumba Hidung Botol Indo-Pasifik di Umah Lumba Rehabilitation Center, Taman Nasional Bali Barat Kristiawan, Vicky; Mahatmi, Hapsari; Sudipa, Putu Henrywaesa; Rahmadani, Deny
Indonesia Medicus Veterinus Vol 11 (2) 2022
Publisher : Faculty of Veterinary Medicine, Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19087/imv.2022.11.2.234

Abstract

Saluran cerna merupakan salah satu tempat pertumbuhan berbagai genus serta spesies bakteri termasuk bakteri family Enterobacteriaceae. Salah satu dari anggotanya adalah Eschericia coli yang merupakan flora normal yang sering ditemukan pada saluran cerna. Saat ini masih sangat langka penelitian tentang keberadaan E. coli pada saluran cerna lumba-lumba hidung botol Indo-Pasifik (Tursiops aduncus). Tujuan penelitian ini adalah untuk mendapatkan data awal mengenai bakteri Enterobateriaceae pada lumba-lumba tersebut yang berada di lingkungan rehabilitasi. Sampel lumba-lumba yang digunakann merupakan lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di jaring apung laut atau seapen Umah Lumba Rehabititation Center, sebanyak tiga ekor. Pengambilan sampel dilakukan dengan menggunakan rubber catheter sterile berukuran 2,7 mm yang dimasukkan ke dalam rektum lumba-lumba. Jumlah total sampel yang di isolasi sebanyak 12 sampel dari tiga ekor lumba-lumba. Isolasi sampel dilakukan dengan menggunakan media Sheep Blood Agar, media Mac Conkey Agar dan Eosine Methylen Blue Agar. Selanjutnya di uji dengan pewarnaan Gram, uji katalase, uji oksidase, Triple Sugar Iron Agar, Sulfide Indole Motility, Methyl Red Voges Proskauer, Simmon Citrate Agar, dan uji urease. Hasil dari penelitian ini dapat disimpulkan pada tiga ekor lumba-lumba hidung botol Indo-Pasifik (T. aduncus) yang berada di seapen Umah Lumba Rehabilitation Center, ditemukan jenis bakteri famili Enterobacteriaceae spesies E. Coli.
Seroprevalence of Q fever in sheep and goat in West Java area Agus Setiyono; Ekowati Handharyani; Hapsari Mahatmi
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 1 (2008): MARCH 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (171.895 KB) | DOI: 10.14334/jitv.v13i1.596

Abstract

Q fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Test
Resistensi Bakteri Aeromonas sp. Isolat Ikan Piranha (Pygosentrus nattereri) Bali Safari And Marine Park terhadap Antibiotik Ni Made Herawati; Hapsari Mahatmi; I Nengah Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (187.629 KB)

Abstract

Piranha (Pygosentrus nattereri) is a fish that has been conserved at Conservation Center of Bali Safari and Marine Park. In May 2012 there was a case that twelve Piranhas suffered from illness and two of them died without any specific clinical signs. The dead Piranhas were necropsied and the tissues or parts of organs were collected for microbial examination interests. Spesies specific bacterial examination was also included using Microgen GNA TM + B-ID system test The bacterial examination found that the fishes were infected by Aeromonas hydropila and Aeromonas veronii. Further examination about antibiotic resistance tests showed that those Aeromonas spp were resistant to chloramphenicol and a combination of trimethoprim and sulphamethoxazole. It can be suggested that, next, the usage of other antibiotics is strongly recommended for the case of Piranha infected by Aeromonas spp at Bali Safari and Marine Park.
Pola Resistensi Salmonella typhi yang Diisolasi dari Ikan Serigala (Hoplias malabaricus) terhadap Antibiotik Waode Santa Monica; Hapsari Mahatmi; Kerta Besung
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.126 KB)

Abstract

The purpose of this study was to know the resistant pattern of Salmonella typhi (S. typhi) isolated from wolf fish (Hoplias malabaricus) to antibiotics in Bali Safari and Marine Park. S. typhi was isolated from fecal swab collected from the illness fish. The S. typhi was confirmed with MicrogenTM GN A ID test’s method. The antibiotic sensitivity of the S. typhi was tested using streptomycin, gentamicin, penicillin, vancomycin, and chloramphenicol according to the Kirby bouer method. The results showed that the isolate of S. typhi was resistant to penicillin and vancomycin, but sensitive to streptomycin, gentamicin, and chloramphenicol.
Detection of Mycobacterium bovis and Klebsiella pneumoniae at Bali Cattle Slaughterhouse by culture analysis and PCR I Gede Oka Darsana; I Nyoman Dibia; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 3 No 2 (2015)
Publisher : Udayana University

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Abstract

A study to determine the presence and prevalence of bovine tuberculosis and Klebsiella pneumoniae atcattle slaughterhouse in Bali was carried out in Pesanggaran and Mambal abattoirs from January to March2015.The Lungs and lymph nodes were inspected for lesions and then examined through DNA analysis forMycobacterium bovis and culture for isolation of Klebsiella pneumoniae. Of the samples examined, no onehad lesions suggestive of tuberculosis in two abattoirs.However, Klebsiella pneumoniae was observed1/4513 sample (0.02%) only in Mambal abattoir. This study is the first report of the presence of Klebsiellapneumoniae in Bali. Therefore, a aware of this zoonotic Klebsiellosis and its control managementformulation are need.
Deteksi Antibodi Mycobacterium tuberculosa bovis pada Sapi di Wilayah Kabupaten Buleleng, Bangli, dan Karangasem Provinsi Bali Putu Gede Widiarsa Putra; Nengah Kerta Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol. 1 No. 1 2013
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (162.55 KB)

Abstract

Bovine tuberculosis (BTB) is one the most widespread zoonotic diseases and has recently reemerged as a major health concern. The disease is caused by the Mycobacterium tuberculosis complex (MTBC) that primarily affects humans as its main host, and that is able to attack more hosts such as domestic animals, pet animals, and wild animals. This Cross-Sectional Study was carried out to determine the seroprevalence of BTB in Districts of Buleleng, Bangli, and Karangasem Province of Bali. One hundred and eighty cow blood samples were collected, and the serological status of BTB was detected using Elisa method. The results showed that the seroprevalence of BTB in District of Bangli was 2.22%, while, those was negative in Districts of Buleleng and Karangasem. The positive seroprevalence of BTB in Bangli indicates that the same research must be conducted throughout the districts in the Province of Bali.
Kepekaan Eschericia coli Patogen yang Diisolasi dari Babi Penderita Kolibasilosis terhadap Antibiotik di Kecamatan Kerambitan dan Tebanan Kabupaten Tabanan, Bali Aditya Nugraha; NK. Besung; Hapsari Mahatmi
Veterinary Science and Medicine Journal Vol 1 No 2 (2013)
Publisher : Udayana University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.275 KB)

Abstract

This study aims to determine the sensitivity pattern of E. coli isolated from pigs with colibacillosis to antibiotics. The total of 21 fecal samples was collected from pigs with white scour at the District of Kerambitan and Tabanan, the Regency of Tabanan, Bali. The isolation and identification of pathogenic E coli used Sheep Blood Agar medium, and serotype grouping of those bacteria was designated through agglutination test using serum’s antibody Polyvalent 1-5 and 6-11. The pathogenic E. coli found was then examined it`s own resistance to streptomycin, sulfamethoxazole, gentamicin, cefotaxime, and chloramphenicol according to Kirby Bouer method. The result showed that the isolates of E. coli polyvalent 1-5 were resistant to streptomycin and chloramphenicol, whereas that of polyvalent 6-11 was resistant to sulfamethoxazole, gentamicin, streptomycin and chloramphenicol.
Transformasi UMKM Perikanan di Kabupaten Badung Melalui Pelatihan Pemasaran Digital dengan Aplikasi www.tokoikan.id Kartika, Gde Raka Angga; Dewi, Ayu Putu Wiweka Krisna; Ernawati, Ni Made; Mahatmi, Hapsari; Taradhipa, I Gusti Agung Dwikhy Oka; Astriani, Ni Luh Ayu Gita
Sricommerce: Journal of Sriwijaya Community Services Vol. 5 No. 2 (2024): Sricommerce: Journal of Sriwijaya Community Services
Publisher : Faculty of Economics, Universitas Sriwijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29259/jscs.v5i2.194

Abstract

Kabupaten Badung sebagai daerah yang memiliki jumlah UMKM Perikanan yang cukup banyak menghadapi tantangan dalam pengembangannya, salah satu diantaranya adalah proses pemasaran produk yang banyak memiliki kendala. Hal ini dikarenakan sebagian besar UMKM perikanan di Kabupaten Badung masih menggunakan sistem konvensional dalam proses penjualan produknya, sehingga kesulitan untuk memasarkan produknya dalam skala lingkup yang lebih luas. Perkembangan teknologi yang cukup pesat telah memberikan peluang bagi pelaku industri dan UMKM perikanan untuk mengembangkan pasarnya melalui penjualan online serta adanya implementasi dari kebijakan pemerintah tentang revolusi industri 4.0. Oleh karena itu dalam mencapai hal tersebut dilakukan kegiatan pengabdian ini, yang memiliki tujuan memberikan pemahaman kepada UMKM perikanan di Kabupaten Badung mengenai pentingnya proses pemasaran digital saat ini dengan cara memberikan pelatihan tentang cara dan kiat untuk memasarkan produk perikanan yang dihasilkan oleh UMKM melalui salah satu pasar digital yaitu www.tokoikan.id. Aplikasi pasar digital www.tokoikan.id merupakan salah satu hasil produk hilirasi dari kegiatan pengabdian di Fakultas Kelautan dan Perikanan Universitas Udayana, yang bertujuan untuk memberikan ruang kepada UMKM perikanan untuk dapat melakukan penjualan digital. Sasaran kegiatan pengabdian masyarakat ini adalah UMKM sektor perikanan di kabupaten Badung, dan pelaksanaan Kegiatan pengabdian ini berkolaborasi dengan Dinas Perikanan Kabupaten Badung. Metode kegiatan pengabdian masyarakat dilakukan dengan pelatihan dan pendampingan kepada UMKM perikanan Kabupaten Badung untuk melakukan proses pemasaran digital melalui aplikasi www.tokoikan.id. Hasil kegiatan pengabdian menunjukkan bahwa UMKM perikanan di Kabupaten Badung mulai memahami pentingnya perubahan pola pemasaran dari cara konvensional menuju ke metode pemasaran digital, hal ini di kuatkan dengan adanya beberapa UMKM yang langsung memulai proses pemasaran digital dengan membuat akun penjual di aplikasi tokoikan.id
CHARACTERISTICS OF PH AND NUMBER OF MICROBES IN THE RETICULUM, OMASUM AND ABOMASUM OF BALI CATTLE I Gusti Ketut Suarjana; I Ketut Tono Pasek Gelgel; I Nengah Kerta Besung; Hapsari Mahatmi; Putu Henrywaesa Sudipa
Buletin Veteriner Udayana Bul. Vet. Udayana. February 2024 Vol. 16 No. 1
Publisher : Fakultas Kedokteran Hewan Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/bvu.v16i1.111

Abstract

Bali cattle are native Indonesian cattle germplasm which must be preserved and cultivated. The research aims to determine the pH characteristics and number of microbes in the reticulum, omasum and abomasum of Bali cattle. The research sample was the stomach contents of the reticulum, omasum and abomasum of clinically healthy Bali cattle slaughtered at the slaughterhouse, Pesanggaran, Denpasar. Samples were taken aseptically from 30 cows. This research is observational with a cross-sectional study design. The number of bacterial and fungal colony populations was calculated using the pour plate method on nutrient agar plates and Sabouraud dextrose agar plates, respectively. The population of Enterobacteriaceae bacterial colonies was counted using the scatter method on Eosin Methylen Blue agar plates. pH measurements in cow stomach contents were carried out using pH indicator strips. The research data were analyzed descriptively. The results showed that the average pH of the reticulum, omasum and abomasum were 7,66; 6,50 and 5,26. The average total bacteria or ALTB in the reticulum, omasum and abomasum were 18,53 x 106 CFU/gr, 21,43 x 106 CFU/gr and 26,73 x 106 CFU/gr; the fungal population was 7,10 x 105 CFU/gr, 4,56 x 105 CFU/gr and 2,83 x 105 CFU/gr; Coliform counts were 60,03 x 103 CFU/gr, 57,70 x 103 CFU/gr and 61,60 x 103 CFU/gr; the number of non-Coliforms was 49,00 x 103 CFU/gr, 45,53 x 103 CFU/gr and 41,46 x 103 CFU/gr, the number of E.coli respectively 20,70 x 103 CFU/gr, 22, 83 x 103 CFU/gr and 22,86 x 103 CFU/gr.
Co-Authors Aditya Nugraha Agus Setiyono Aida Lousie Tenden Rompis Anak Agung Sagung Rani Manika Putri Arifah, Sitti Astriani, Ni Luh Ayu Gita Ayu Putu Wiweka Krisna Dewi, Ayu Putu Wiweka Krisna BOI DARMA Darmawan, Alpian Deny Rahmadani, Deny Ekowati Handharyani EMILIUS MELIANO UMBU RAZA Fachriyan Hasmi Pasaribu Fitri Indah Permatasari Gde Raka Angga Kartika GUSTI AGUNG AYU ANGGRENI PERMATASARI I Gede Hendra Prasetya Wicaksana I GEDE OKA DARSANA I Gede Oka Darsana I Gusti Ketut Suarjana I Ketut Suada I Ketut Tono Pasek Gelgel I Made Arthawan I Nengah Kerta Besung I Nengah Kerta Besung I Nyoman Dibia I Wayan Sudira K. Tono P.G Kadek Apriyan Widiarta KETUT SUADA Ketut Tono Pasek Gelgel Kristiawan, Vicky MADE SUMITHA KAMESWARI MADE SUMITHA KAMESWARI MADE YENDHI SAWITTI MARIA PRISTI ANRIS YUNIKAWATI Muh. Muazdzamzam Lil Abrori Muslimin, Lucia N. Suarsana Ni Made Ernawati, Ni Made Ni Made Herawati Putu Gede Widiarsa Putra Putu Henrywaesa Sudipa Putu Henrywaesa Sudipa Retno Damayanti Soejoedono REZKI ABADI BONTONG Syafiana Fairizca Taradhipa, I Gusti Agung Dwikhy Oka Vera Paulina Sitanggang Vera Paulina Sitanggang WAODE SANTA MONICA