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Journal : unej e-proceeding

Optimisation of Extractant and Extraction Time on Portable Extractor Potentiometric Method for Determining Phosphate in Soil Anggia Rose Sukaton; Siswoyo Siswoyo; Bambang Piluharto
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Phosphorus is one of the nutrients which is needed by plants in large amounts. Fertilisation is a popular solution for supplying a sufficient amount of phosphorus in the soil. However, phosphorus can pollute water bodies and lead to eutrophication if fertilisation is carried out without considering prior information about the soil condition. Thus, analysis of phosphorus needs to be done before fertilisation. This paper is reporting a result in development an alternative strategy for analysing the phopsphorus in soil agriculture using potentiometric method. The aims of this research were to find the optimum condition of extractant and extraction time for determining phosphate, one form of phosphorus, in soil and evaluate a portable extractor potentiometric (PEP) method for determining phosphate in soil. Measurement of phosphate was undertaken by using cobalt working electrode, silver-silver chloride reference electrode and 0,025 M potassium hydrogen phtalate at pH 4 as ionic strength adjuster. Evaluation of PEP method was carried out by comparing this method with both the conventional potentiometric (CP) and the standard spectrometric (SS) methods. It was found that Kelowna was the optimum extractant for measuring phosphate potentiometrically in soil by CP method. Extraction time of 10 minutes was the optimum time for extracting phosphate in soil using Kelowna extractand solution. The result also shown that correlation between PEP and CP method was 0.883 whereas correlation between PEP and SS method was 0.924. Linear response characterisation of both PEP and CP method has obtained slope of (-28.47) and (-23.67) mV per decade respectively, in other hand the PEP and CP has resulted the detection limit 0.72 ppm and 0.83 ppm respectively. Repeatability for both PEP and CP method were less than 5%.
High Performance Liquid Chromatography of Amino Acids Using Potentiometric Detector With A Tungsten Oxide Electrode Yeni Maulidah Muflihah; Zulfikar Zulfikar; Siswoyo Siswoyo; Asnawati Asnawati; Qurrota Ayun
UNEJ e-Proceeding 2016: Proceeding The 1st International Basic Science Conference
Publisher : UPT Penerbitan Universitas Jember

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Abstract

High Performance Liquid Chromatography (HPLC) method combine with potentiometric detector was applied as a method for analysis of amino acids (aspartic acid, glutamic acid and arginine). The separation was carried out on a C18 column with isocratic elution of  a mixture of acetonitrile and phosphat buffer. Potentiometric detector using a tungsten oxide electrode as working electrode and Ag/AgCl as refference electrode. The detection method was based on the presence of H+ ions from amino acids. This application was a development of previous studies that was succesfully applied to detect the presence of amino acids in batch and flow systems. Amino acid determination was characterized by its retention time. Retention time of aspartic acid, glutamic acid and arginine were as follow: 8,46; 13,0; and 15,21 minutes. The optimized separation conditions obtained at a flow rate of 1,2 mL/min with 15% acetonitrile concentration, buffer pH 6,5 with phosphat concentration of 5x10-4 M. Detector performance tested by the recovery test of samples, and the results obtained for glutamic acid, aspartic acid and arginine respectively: 89,1%, 94,9%, and 110%. Linear range obtained at 10-3 M to 10-7 M.  Detection limit were 1,58x10-7 M for glutamic acid, 6,58x10-8 M for aspartic acid and 6,51x10-8 M for arginine.
KNOWLEDGE OF CLEAN AND HEALTHY LIFE BEHAVIOR WITH TRACOMA PREVENTION MEASURES IN THE ELDERLY Siswoyo Siswoyo; Kushariyadi Kushariyadi; Setyo Bagus Hariyanto
UNEJ e-Proceeding 2023: 5th International Agronursing Conference (5th IANC) In Conjunction with 1st International Post
Publisher : UPT Penerbitan Universitas Jember

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Abstract

Background: The problem of a person's lack of knowledge about eye diseases can cause a lack of understanding in the community. Elderly who live with their families do not know how to live a clean and healthy life. Implementation of clean and healthy living behavior in the household order by the community is still below standard. The aims to analyze knowledge of clean and healthy living behavior with trachoma disease prevention measures in the elderly. Methods: The research design used cross-sectional, namely measuring knowledge of clean and healthy living behavior towards prevention of trachoma in the elderly. Sample research, namely elderly who are still healthy who are in the working area of ​​the Patrang Public Health Center as many as 52 respondents. Through a purposive sampling technique with inclusion criteria including: 1) elderly aged 50-85 years and carried out screening using the MMSE questionnaire; 2) can communicate well and smoothly; 3) cooperative elderly; 4) willing to be a respondent. Researchs data obtained from the results of filling out questionnaires by respondents. Then the data is tested for analysis Rank Spearman’s correlation using the SPSS statistic with a p value <0.05. Results: The research results show the knowledge of clean and healthy living behavior is mostly in the sufficient category as many as 26 respondents (50%). Trachoma disease prevention measures in the elderly are mostly in the good category as many as 33respondents (63%). There is a relationship between knowledge of clean and healthy living behavior and prevention of trachoma in the elderly, with a p = value 0.001, and value r = 0.543 means that it has a positive or unidirectional relationship with moderate correlation strength. Conclusions: Knowledges of clean and healthy living behavior can shape a person's actions or behavior for the better. Knowledge of clean and healthy living behavior can be learned and or can be obtained through information from various sources and methods (print or electronic form). So that can take preventive measures against trachoma in the elderly.