Claim Missing Document
Check
Articles

Found 2 Documents
Search
Journal : Sainstek

ISOLASI DAN KARAKTERISASI SENYAWA ALKALOID DARI DAUN ALPUKAT (PERSEA AMERICANA MILL) Tengo, Nilda Apriyati; Bialangi, Nurhayati; Suleman, Nita
Sainstek VOL 07, NO 01, 2013
Publisher : Sainstek

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (265.762 KB)

Abstract

The aims of this study is to isolate and find the alkaloid compounds contained in the leaves of avocado (Persea americana Mill). The method used is the isolation of pure compounds to obtain isolates. 400 g of avocado leaves macerated with methanol solvent yield 53.41 gr of methanol extract. The results of column chromatography I yield 17 fractions, the fraction N12 is re-elected for column chromatography II. The results of chromatography N12 obtained isolate pure in fractions 7. This isolate is tested purity by using thin layer chromatography with eluent variations produce a single stain pattern, so it can proceed with the test isolate phytochemicals. The results of IR spectrophotometer data interpretation, isolate pure fractions 7 indicates the presence of alkaloid compounds having functional groups N-H (3311.55 cm-1), C-H aliphatic (2921.96 cm-1, 2850.59 cm-1), C=O (1735.81 cm-1, 1641.31 cm-1), C-N (1240.14 cm-1, 1130.21 cm-1), C-H Aromatic (910.34 cm-1, 846 cm-1), N-C=O (580.53 cm-1). The results of UV-Vis spectrophotometer from fractions 7 gives absorption wavelength of 238.5 nm indicating the occurrence of electron transition n * and n * which indicates the existence of C=O and N-H groups.
FITOREMEDIASI PADA MEDIA TANAH YANG MENGANDUNG Cu DENGAN TANAMAN KANGKUNG DARAT Haruna, Elvira T; Isa, Ishak; Suleman, Nita
Sainstek VOL 06, NO 06, 2012
Publisher : Jurnal Sainstek

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (118.337 KB)

Abstract

One of the restoration of contaminated environmental quality method is using phytoremediation techniques. That is recovery contaminated environment using plants. This research studied the ability of kangkung darat (Ipome reptans) to absorb metallic copper and to find out which part of the Kangkung Darat that absorb most of the metal Cu, also to know the difference between absorption and kangkung darat are harvested at 2, 4 and 6 weeks. The research activities are carried out by planting kangkung darat in a contaminated soil media and in the flush of copper metal with a solution of Cu mg / L two times a day. Samples of kangkung darat have been taken from accordance with plant age (2, 4 and 6 weeks), and plant parts (roots, stems and leaves). Used in Atomic Absorption Spectroscopy (AAS) Simatzu type AA to measure the content of Cu in the sample. Results obtained from this study that Cu accumulation in plants is mainly found in the roots. On the kangkung darat were 2 weeks of Cu contained as much as 5.3403 ppm in the roots, stems and leaves of 2.6637 5.1117. At the age of 4 weeks in a row at the root, stem leaves are 10.6998, 5.8281 and 2.0666 ppm. Mean, at the age of 6 weeks at the root of 10.4605, the leaves on the stem 7.7839 and 9.1863 ppm. From the research results can be concluded that kangkung darat could be used as fitoremediator Cu metal contaminated soil.