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All Journal Indonesian Journal of Tropical and Infectious Disease Journal Of Vocational Health Studies Qanun Medika - Medical Journal Faculty of Medicine Muhammadiyah Surabaya J-Dinamika: Jurnal Pengabdian Kepada Masyarakat JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Wahyunitisari, Manik Retno
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Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Public Health

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Bacterial Profile and Antibiotic Resistance Pattern among Children with Urinary Tract Infections in Dr. Soetomo Hospital, Surabaya, Indonesia Adhima, Fauziah; Wahyunitisari, Manik Retno; Prasetyo, Risky Vitria; Setiabudi, Rebekah Juniati
Indonesian Journal of Tropical and Infectious Disease Vol. 10 No. 2 (2022)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijtid.v10i2.32908

Abstract

Urinary tract infections (UTIs) are the most common infections in pediatric patients characterized by the growth of bacteria in the urine in significant numbers. Antibiotics remain the primary treatment of UTI in children. However, there has been an increase in antibiotic resistance to uropathogens worldwide due to their inappropriate and extensive uses. There is considerable geographical variation in the distribution of bacteria and antibiotic resistance pattern. Thus, to prevent further resistance and provide empirical antibiotic options, this study aims to determine the profile of bacteria and antibiotics resistance pattern among UTI pediatric patients in Dr. Soetomo Hospital. This study was performed by collecting data from the urine culture logbook at the Clinical Microbiology Laboratory of Dr. Soetomo Hospital in July-October 2019. The sample was UTI patients aged one day – 18 years due to bacterial infection with a colony count of   ≥100,000 CFU/ml. In this study, 131 patients showed significant bacterial growth dominated by males and ages one month – 2 years. UTI were caused by gram-negative bacteria (74%) and gram-positive bacteria (26%), with the most bacteria found in each group were Escherichia coli and Enterococcus faecalis. E. coli showed ≥70% resistance to ampicillin, cefazoline, piperacillin, tetracycline, and trimethoprim-sulfamethoxazole. Comorbidities were dominated by hydronephrosis (10.98%), chronic kidney disease (9.79%) and hydrocephalus (8.09%). In conclusion, gram-negative bacteria were the leading cause of UTI in children with E. coli as the most common uropathogen, highly resistant to ampicillin and cefazolin. Gram-positive bacteria were less frequent with varied resistance patterns. Most common comorbidity was hydronephrosis.
Cavendish Banana Peel Extract's Antibacterial Activities Potential as Disinfectant Ramadhan, Erlangga Lazuardi; Retnowati, Wiwin; Dewanti, Linda; Wahyunitisari, Manik Retno
JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Vol. 14 No. 2 (2023): Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/juxta.V14I22023.100-104

Abstract

Highlights:1. The COVID-19 pandemic makes personal hygiene more important than ever, and antibacterial substances such as disinfectants are crucial in maintaining said hygiene.2. The MBC of cavendish banana peel extract against the growth of bacteria Escherichia coli and Staphylococcus aureus was 25%, with no activity against Bacillus subtilis.3. Musa acuminata peel extract has the potential to be used as a disinfectant. AbstractIntroduction: The COVID-19 pandemic makes personal hygiene more important than ever, and antibacterial substances such as disinfectants are crucial in maintaining said hygiene. This study aimed to determine the minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) of cavendish banana peel extract (Musa acuminata) against Staphylococcus aureus, Escherichia coli, and Bacillus subtilis.    Methods: The design of this study was a laboratory experiment that used the broth dilution method with test tubes using methanol as the extract's solvent. Sterile aquadest was used as the solvent, and Mueller-Hinton broth was used as the growth medium in tubes. All samples of the bacteria (Staphylococcus aureus, Escherichia coli, and Bacillus subtilis) were provided by the Laboratory of Microbiology Faculty of Medicine, Universitas Airlangga.Results: MBC was the only parameter found due to the color and particulates, which hindered the turbidity assessment of MIC. From the dilution test, the MBC of cavendish banana peel extract against the growth of bacteria Escherichia coli and Staphylococcus aureus was 25%, with no activity against Bacillus subtilis.Conclusion: There were antibacterial activities of Musa acuminata peel extract against Staphylococcus aureus and Escherichia coli. Therefore, it has the potential to be used as a disinfectant.
INHIBITORY ACTIVITY OF ZINGIBER OFFICINALE VAR RUBRUM EXTRACT AGAINST STAPHYLOCOCCUS AUREUS Dewi, Firnanda Iptita; Wahyunitisari, Manik retno
Journal of Vocational Health Studies Vol. 1 No. 3 (2018): March 2018 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V1.I3.2018.113-116

Abstract

Background: gram-negative bacteria-salmonella typhi and gram-positive bacteria-staphylococcus aureus are types of bacteria that cause infectious disease among several areas in Indonesia. Staphylococcus aureus often found in skin lesions injury. Red ginger extract (Zingiber officinale var rubrum) has been known for its medicinal value especially as an antibacterial agent. Purpose: This study aims to test the ability of Zingiber officinale var rubrum on inhibiting the growth of Staphylococcus aureus. Methods: the activity of the antibacterial agent was calculated based on Minimum Inhibitory Concentration (MIC) using dilution method. Minimum Inhibitory Concentration of extract was determined by incubating bacteria with the extract overnight at 37 0C. Changes in turbidity and inhibition on bacterial growth were then observed. Result: The results show that minimum concentration for inhibitory effect was 1000 μg/mL. Conclusion: extract of Zingiber officinale var rubrum have an inhibitory effect on the growth of Staphylococcus aureus but no activity of killing bacterial was observed on the concentration given.
MICROBIAL PATTERNS OF HOSPITALIZED HIV POSITIVE PATIENTS ADMITTED IN DR. SOETOMO GENERAL HOSPITAL, SURABAYA INDONESIA Tan, Feriawan; Triyono, Erwin Astha; Wahyunitisari, Manik Retno
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.1-11

Abstract

Background: HIV patients with a weak immune system are very vulnerable to opportunistic infections, can trigger systemic endothelial activation and end up as a condition of sepsis. In Indonesia currently, there is no bacterial epidemiological mapping of the etiology of opportunistic infections in HIV patients. Purpose: To determine the pattern of bacteria that cause opportunistic infections and their antibiotic sensitivity in HIV patients. Method: Prospective observational study design. Data were obtained from medical records of hospitalized patients at RSUD Dr. Soetomo Surabaya from August 2019 - February 2020. Result: Out of 64 patients, 83 specimens were found with the most types of gram-negative bacteria 44.6%, while gram-positive bacteria were 15.7% and a mix of 2.4% and 37.3% negative culture. The highest prevalence of gram-negative bacteria was Klebsiella pneumoniae (35.15), followed by Escherichia coli (10.8%), Pseudomonas aeruginosa (8.1%), and Acinetobacter baumanii (8.1%). The highest prevalence of gram-positive bacteria was Streptococcus mitis / oralis (30.7%), followed by Staphylococcus aureus (23.1%) and Staphylococcus epidermidis (15.4%). Among gram-negative bacteria antibiotic, Cefoperazone-sulbactam showed the greatest sensitivity, following by Amikacin, Gentamycin and Piperacillin-tazobactam; while among gram-positive bacteria are Chloramphenicol, Linezolid, and Vancomycin. Almost all isolates showed resistance to Ampicillin. Conclusion: Bacteria pattern that caused opportunistic infection in RSUD Dr. Soetomo is K. pneumonia as most common gram-negative bacteria followed by E. coli, P. aeruginosa and A. baumanii; while the most gram-positive bacteria found are S. mitis/oralis and S. aureus. Among antibiotic used, Ampicillin showed the lowest sensitivity to almost all bacteria isolates.
MRSA COLONITATION DETECTION IN OBJECT NEAR PATIENTS IN BURN UNIT RSUD DR. SOETOMO - INDONESIA Wahyunitisari, Manik Retno; Mustikasari, Melisa Indah; Hariani, Lynda
Journal of Vocational Health Studies Vol. 5 No. 1 (2021): July 2021 | JOURNAL OF VOCATIONAL HEALTH STUDIES
Publisher : Faculty of Vocational Studies, Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jvhs.V5.I1.2021.22-25

Abstract

Background: Burn unit is a unit where patients with a burn wound in which the patient's skin is not intact are taken care of. Methicillin-resistant Staphylococcus aureus (MRSA) as the prototype of a nosocomial pathogen is usually transmitted through contact from the surfaces of the object to the non-intact skin. Therefore, the possibility of MRSA infection increase in the burn unit. Purpose: The purpose of this research is to find out whether there is any colonization of MRSA on the objects around burn unit patients that can contribute to the spreading of MRSA in the RSUD Dr.Soetomo Indonesia. Method: The sampling period was started from October 2019 until March 2020. Samples were taken randomly from 28 patients in the Burn unit RSUD Dr.Soetomo -ndonesia and then tested for identification in the microbiology laboratory of the medical faculty, Universitas Airlangga. Result: Nineteen bacterial growths were obtained from twenty-eight samples taken, nine of them were confirmed as Stahylococcus aureus and one from nine of them was confirmed as MRSA. Conclusion: MRSA contamination was found on an object around the patient burn unit Dr. Soetomo-Indonesia which comes from the stethoscope membrane.
Pemberdayaan Guru Yayasan Pendidikan Al Khairaat Yogyakarta pada Penanggulangan Antimicrobial Resistance: dari UNAIR untuk Indonesia Wahyunitisari, Manik Retno; Koendhori, Eko Budi; Endraswari, Pepy Dwi; Purwono, Priyo Budi; Juniastuti, Juniastuti; Wiqoyah, Nurul; Sardjono, Lindawati Alim; Vermasari, Naritha; Muna, Nafdzu Makhmudatul; Setiawan, Firman; Lindarto, Wira Widjaya; Widyatama, Fikri Sasongko
J-Dinamika : Jurnal Pengabdian Masyarakat Vol 9 No 3 (2024): Desember
Publisher : Politeknik Negeri Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25047/j-dinamika.v9i3.5481

Abstract

Antibiotics are compounds created by microorganisms to live by destroying their competitors. Antibiotics are commonly used without a prescription to treat symptoms caused by non-bacterial infections. Antibiotic resistance is a global problem. Continuous education is needed by agents of change to overcome this problem. Community service collaboration between the Department of Microbiology-Parasitology, FK Unair, and the Department of Microbiology, Faculty of Medicine, Public Health, and Nursing, Gadjah Mada University with partnerships for the goals teachers from the Al Khairaat Yogyakarta educational foundation, aim to provide an understanding of the proper use of antibiotics. The initial target of transfer of knowledge was successfully measured by increasing participants' post-test results.
Analysis of Neutrophil, Leukocyte, Lymphocyte, and NLR Cell Profiles in Patients Diagnosed with Chronic Gastritis Affected by Helicobacter pylori and Non-Helicobacter pylori Infection at Dr. Soetomo Hospital in Surabaya, Indonesia Aziz, Nahdah Aulia; Wahyunitisari, Manik Retno; Susilo, Imam
Qanun Medika - Jurnal Kedokteran FK UMSurabaya Vol 9 No 01 (2025): Qanun Medika Vol 09 No 01 January 2025
Publisher : Universitas Muhammadiyah Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30651/jqm.v9i01.21260

Abstract

Helicobacter pylori (HP) is a prevalent cause of bacterial infections, particularly chronic gastritis in the human body. The diagnosis of gastritis relied on gastric biopsy examination, where inflammation is characterized by an increase in various inflammatory cells, including macrophage, neutrophil, lymphocyte, or plasma cells. Therefore, this research aimed to determine the profiles of neutrophil, leukocyte, lymphocyte, and neutrophil lymphocyte ratio (NLR) in chronic gastritis patients, distinguishing between those with Helicobacter pylori and non-Helicobacter pylori infection at Dr. Soetomo Hospital in Surabaya. This research adopted a retrospective method, using medical records for chronic gastritis patients at Dr. Soetomo Hospital between 2018 and 2022. The variables analyzed included Helicobacter pylori infections and values of neutrophil, leukocyte, lymphocyte, and NLR cells. The obtained data were subjected to analysis using descriptive statistics. The results showed that the majority of participants were females (61.9%), senior high school graduates (52.38%), and aged 45-59 (pre-elderly) (32.14%). In non-Helicobacter pylori group, the average values of neutrophil, leukocyte, lymphocyte, and NLR cells were 62.29 (SD = 12.17), 8.155 (SD = 4.02), 29.165 (SD = 12.31), and 3.68 (SD=4.865), respectively. In conclusion, females showed a propensity for chronic gastritis with Helicobacter pylori. It was observed that non-Helicobacter pylori chronic gastritis tended to have elevated neutrophil and leukocyte values. Meanwhile, Helicobacter pylori-related chronic gastritis tended to show increased lymphocyte and NLR values due to systemic inflammation. Helicobacter pylori was associated with conditions such as iron deficiency anemia, cardiovascular disease, diabetes, thyroid disease, and malnutrition.
Co-Authors Adhima, Fauziah Aziz, Nahdah Aulia Dewi, Firnanda Iptita Eko Budi Koendhori, Eko Budi Erwin Astha Triyono Fikri Sasongko Widyatama Hariani, Lynda Imam Susilo Juniastuti Juniastuti Linda Dewanti Lindarto, Wira Widjaya Muna, Nafdzu Makhmudatul Mustikasari, Melisa Indah Nurul Wiqoyah, Nurul Pepy Dwi Endraswari, Pepy Dwi Priyo Budi Purwono Ramadhan, Erlangga Lazuardi Rebekah Setiabudi, Rebekah Rehana, Anindita Risky Vitria Prasetyo Sardjono, Lindawati Alim Setiawan, Firman Sulistiawati Sulistiawati Tan, Feriawan Vermasari, Naritha Wiwin Retnowati