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Keragaman Genetik Aksesi Tanaman Kunyit (Curcuma longa L.) Berdasarkan Penanda Molekuler Simple Sequence Repeat (SSR) Muthia Hanin Afidani; Noor Farid; Eka Oktaviani
Vegetalika Vol 14, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.99927

A plant breeding program for turmeric (Curcuma longa L.) development is required to fulfil the pharmaceutical industry's demand. Understanding genetic diversity is the initial step in selecting the parents in plant breeding. Selection assisted by molecular markers, specifically Simple Sequence Repeat (SSR), can be used to obtain genetic information. However, genetic information on turmeric based on SSR markers in Indonesia is still limited. This study aimed to determine the genetic diversity and relationship of six accessions of turmeric using 4 (four) SSR primers. This study was conducted from October 2023 to January 2024 at the Laboratory of Plant Breeding and Biotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman. The result showed that the CuMiSat-19, CuMiSat-20, and CuMiSat-29 primers were informative based on the electrophoresis band polymorphism. The CuMiSat-19 primer generated the highest PIC value (0,88), followed by CuMiSat-20 (0,80) and CuMiSat-29 (0,74). While CuMiSat-23 did not produce polymorphism, the PIC value was 0. The dendrogram classified six turmeric accessions into the two clusters with a Jaccard correlation coefficient range of 0.11-0.33. Cluster I consisted of accession A (Ponorogo), accession C (Gresik), accession D (Indramayu), and accession B (Semarang). Cluster II grouped accession E (Banyumas) and accession F (Bogor). Based on the polymorphism and similarity coefficient, turmeric accessions have a wide range of genetic diversity. Turmeric in different clusters is a potential germplasm that can be utilized to broaden genetic diversity in plant breeding programs.

PARAMETER GENETIK AKSESI JAHE MERAH (ZINGIBER OFFICINALE VAR. RUBRUM) YANG DIKOLEKSI DARI PULAU JAWA, INDONESIA Oktaviani, Eka; Agleri Rante; Agus Suroto
Jurnal Agrotek Tropika Vol. 13 No. 4 (2025): JURNAL AGROTEK TROPIKA VOL 13, NOVEMBER 2025
Publisher : Departement of Agrotechnology, Agriculture Faculty, Universitas Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jat.v13i4.8344

Jahe merah merupakan jahe yang lebih pedas dibandingkan dengan jenis jahe lainnya, yang dapat dimanfaatkan dalam industri obat-obatan. Keterbatasan bibit menjadi permasalahan utama dalam budidaya jahe merah. Informasi tentang keragaman genetik ddan fenotipik jahe merah diperlukan untuk menentukan metode terbaik dalam menghasilkan varietas unggul baru melalui program pemuliaan tanaman. Penelitian ini bertujuan untuk mengetahui keragaman genotipe, fenotipe, dan heritabilitas 7 (tujuh) aksesi jahe merah yang dikoleksi dari 7 (tujuh) daerah di Pulau Jawa. Hasil penelitian menunjukkan bahwa 7 (tujuh) aksesi jahe merah yang diteliti, berdasarkan 4 (empat) karakter agronomi kuantitatif yang diamati, mempunyai ragam genetik yang sempit dan ragam fenotipe yang luas. Berdasarkan nilai duga heritabilitas, diketahui bahwa tinggi tanaman dipengaruhi oleh faktor genetik, sedangkan luas daun, jumlah daun, dan diameter batang dipengaruhi oleh faktor genetik dan lingkungan.

Increasing Interest and Knowledge about Verticulture Cultivation among Adolescents at the Gelora Indah Mosque Orphanage, North Purwokerto: Peningkatan Minat dan Pengetahuan tentang Budidaya Vertikultur pada Remaja Panti Asuhan Masjid Gelora Indah Purwokerto Utara Agus Suroto; Tarjoko; Eka Oktaviani
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol. 8 No. 1 (2024): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : DPD Jatim Perkumpulan Dosen Indonesia Semesta

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Orphanages play an important role in replacing the role of parents in nurturing, looking after, and providing guidance to children so that they become adults who are valuable and responsible for themselves and society in the future. The goal of the community service was to increase the interest and knowledge of teenagers at the Gelora Indah Mosque Orphanage, North Purwokerto, regarding vertical-culture (verti-culture) cultivation techniques. Verticulture technique was chosen because of the potential for narrow yard land, which can be used as land for agricultural crop production, especially plants that are suitable for vertical cultivation. This activity can be used as a provision for achieving food self-sufficiency at the household level and as a basis for producing agricultural products that can be used as input for entrepreneurship from an early age. The community service was conducted from March to May 2023 with all the teenagers of Gelora Indah Mosque Orphanage. The methods were a pre-test, a transfer of knowledge about verti-culture cultivation techniques, cultivation practices, harvesting, and finally, post-tests for the last evaluation. This activity can increase participants' interest and knowledge about the concept of verti-culture by 83.34%, about the needs for verti-culture installation by 66.67%, and about the benefits of verti-culture by 100%. Activities in the form of transfer of knowledge about post-harvest handling and processing, as well as marketing techniques for processed and fresh agricultural products, are needed to support the long-term benefits of this activity.

Increasing of Knowledge and Interest in Vertiminaponic Cultivation for Teenagers in Gelora Indah Mosque Orphanages, Purwokerto Lor: Peningkatan Pengetahuan dan Minat tentang Budidaya Vertiminaponik Remaja Panti Asuhan Masjid Gelora Indah Purwokerto Lor Eka Oktaviani; Agus Suroto; Imastini Dinuriah
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol. 9 No. 1 (2025): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : DPD Jatim Perkumpulan Dosen Indonesia Semesta

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An orphanage is a place of residence and care, both for orphans and for abandoned children. The Gelora Indah Mosque Orphanage in Purwokerto is inhabited by thirteen teenagers. The potential of the second floor attic of this orphanage has not been maximized. With the condition of the yard land that is not spacious and to equip the teenagers with soft skills, one technology that can be applied is the vertiminaponic technique. This technique combines plant cultivation (hydroponics) and fish (aquaculture) in one place/land that is arranged vertically. Community service activities are carried out using the lecture method followed by cultivation assistance. The results of community service show an increase in knowledge and concepts about vertiminaponic cultivation of the orphanage teenagers by 83.34% and 49.58%. Meanwhile, when viewed from the aspect of knowledge about equipment and stages in vertiminaponic cultivation, there is an increase of 65.26% and 70.32%. This activity is an initiation in order to increase food independence for the orphanage teenagers through diversification of processed products from cultivation.

Improving the Knowledge of Women Farmers Group (KWT) on Drip Irrigation System: Innovation in Water Use Efficiency for Sustainable Agriculture in Bandingan Village, Rakit, Banjarnegara: Peningkatan Pengetahuan Kelompok Wanita Tani (KWT) terhadap Sistem Irigasi Tetes : Inovasi Hemat Air untuk Pertanian Berkelanjutan Di Desa Bandingan, Rakit, Banjarnegara Eka Oktaviani; Isnaendi Agung Abadi; Fifi Nafisah; Ravi Izza Alfani; Galuh Muthia Dewi; Anisa Nur Afni Rahmadhani; Nadya Riezqi Rahman
JATI EMAS (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat) Vol. 10 No. 1 (2026): Jati Emas (Jurnal Aplikasi Teknik dan Pengabdian Masyarakat)
Publisher : DPD Jatim Perkumpulan Dosen Indonesia Semesta

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Bandingan Village, Rakit District, Banjarnegara Regency, Central Java Province, has dry lands, resulting in relatively low agricultural productivity. Given these conditions, the drip irrigation system is an alternative solution for efficient water use. The community service activity on the socialization of the drip irrigation system was aimed to increase the knowledge of the women farmers group (KWT) regarding the application of the drip irrigation system. This activity was part of the Community Service Program of Universitas Jenderal Soedirman for the period July - August 2025, which have been carried out through initial observation, assesment (pre-test), the transfer of knowledge about the drip irrigation system, discussions, practices, then final assessment (post-test), and as well as analysis data of the evaluation. Eighteen members of the women farmers group actively participated in this program. Data analysis was carried out using a paired T-test in Microsoft Excel. Overall, the data analysis showed a significant increase in knowledge after the socialization, particularly in understanding (definition), advantages (benefits), tools used for installation, and how drip irrigation works. The increase in knowledge of drip irrigation was 95.83%, the benefits aspect reached 90.28%, the knowledge aspect regarding installation tools was 100%, and the aspect regarding how it works was 88.33%. Thus, this activity increases public knowledge about the concept, benefits, and application of the technology. This knowledge can be applied to improve water use efficiency in the agricultural land that tends to be dry in this village.

Improving Phosphate-Solubilizing Rhizobacteria Strains via Mutation to Enhance Phosphorus Availability in Acidic Soils Nugroho, Adin Heriyan; Putri, Faizzah Kahfi; Sya'diyah, Hamda Hamidatu; Fatmawati, Aprilia; Ghiffar, Hafidho Muntazul; Oktaviani, Eka
Jurnal Ilmu Pertanian Vol 11, No 1 (2026): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.111550

Acid-dry land is a suboptimal land area widely distributed in Indonesia, which has the potential to be developed as cropland to increase crop productivity. However, the low phosphorus availability in this land type often constrains plant growth, necessitating the use of microbial phosphate-solubilizing agents. To address this, the capability of the isolated rhizobacteria strain in phosphate solubilization was improved through a mutagenesis by Ethyl Methane Sulphonate (EMS). Three isolates of indigenous wild-type rhizobacteria of acid-dry land were isolated from cardamom, lemon grass, and bamboo rhizosphere. Mutagenesis of these wild-types yielded three mutant isolates, including KPG_m, SRH_m, and BMB_m, which exhibited higher Phosphate-Solubilization Indices (PSI) compared to their parental strains. The PSI values for the mutants were 2.2, 3.4, and 2.0, respectively, showing a marked improvement over the wild-type values of 2.0, 1.75, and 1.25. These results indicate an enhancement in phosphate-solubilizing ability driven by EMS mutagenesis. All the isolates were then characterized and showed similar traits to those of the Genus of Bacillus. According to the hypersensitive reaction assay, the six isolates showed a negative reaction, indicating they are non-pathogenic. Based on these findings, the mutants have the potential to be developed as biofertilizer agents due to they are non-pathogenic for plants. In addition, the mutants have a moderate PSI value (2<PSI<4) and effective solubilization rate that may improve phosphate solubilization process in acid-dry land.

Keragaman Genetik Aksesi Tanaman Kunyit (Curcuma longa L.) Berdasarkan Penanda Molekuler Simple Sequence Repeat (SSR) Muthia Hanin Afidani; Noor Farid; Eka Oktaviani
Vegetalika Vol 14, No 2 (2025)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/veg.99927

A plant breeding program for turmeric (Curcuma longa L.) development is required to fulfil the pharmaceutical industry's demand. Understanding genetic diversity is the initial step in selecting the parents in plant breeding. Selection assisted by molecular markers, specifically Simple Sequence Repeat (SSR), can be used to obtain genetic information. However, genetic information on turmeric based on SSR markers in Indonesia is still limited. This study aimed to determine the genetic diversity and relationship of six accessions of turmeric using 4 (four) SSR primers. This study was conducted from October 2023 to January 2024 at the Laboratory of Plant Breeding and Biotechnology, Faculty of Agriculture, Universitas Jenderal Soedirman. The result showed that the CuMiSat-19, CuMiSat-20, and CuMiSat-29 primers were informative based on the electrophoresis band polymorphism. The CuMiSat-19 primer generated the highest PIC value (0,88), followed by CuMiSat-20 (0,80) and CuMiSat-29 (0,74). While CuMiSat-23 did not produce polymorphism, the PIC value was 0. The dendrogram classified six turmeric accessions into the two clusters with a Jaccard correlation coefficient range of 0.11-0.33. Cluster I consisted of accession A (Ponorogo), accession C (Gresik), accession D (Indramayu), and accession B (Semarang). Cluster II grouped accession E (Banyumas) and accession F (Bogor). Based on the polymorphism and similarity coefficient, turmeric accessions have a wide range of genetic diversity. Turmeric in different clusters is a potential germplasm that can be utilized to broaden genetic diversity in plant breeding programs.

Seed Bio-Priming to Enhance Seed Germination and Seed Vigor of Rice Using Rhizobacteria from The Northern Coast of Pemalang, Central Java, Indonesia Purwanto Purwanto; Eka Oktaviani; Ni Wayan Anik Leana
PLANTA TROPIKA Vol. 10 No. 2 (2022)
Publisher : Department of Agrotechnology, Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/pt.v10i2.13722

The growth and yield of plants are strongly influenced by the early growth ability of the plants. Similar germination and good seed vigor will greatly support plant growth and increase production. Increasing the germination and vigor of seeds can be done through biopriming. The application of biopriming using rhizobacteria is developing environmentally friendly agricultural technology. This study aimed to determine the effect of inoculation of rhizobacteria from the north coast of Pemalang on rice plants' germination and vigor index. The study was arranged in a Randomized Block Design, consisting of 10 treatments with three replications. Ten rhizobacteria isolates were isolated from the North Coast of Pemalang, Central Java, consisting of Ju1, Jn3, Jn1, J, J12, J5, Kn1, A3, Jn, and K3. The biopriming with rhizobacteria isolated from the rice rhizosphere of the Northern Coast of Pemalang increased the seed germination rate, seed vigor index, and early vegetative growth of rice seedlings. Inoculation with isolate J12 produced the highest vigor index of 8280.01. The results of this study imply that the application of rhizobacteria from saline soil has the potential to increase the vigor of rice seedlings to impact better seedling growth in saline conditions.

Improving Phosphate-Solubilizing Rhizobacteria Strains via Mutation to Enhance Phosphorus Availability in Acidic Soils Adin Heriyan Nugroho; Faizzah Kahfi Putri; Hamda Hamidatu Sya'diyah; Aprilia Fatmawati; Hafidho Muntazul Ghiffar; Eka Oktaviani
Jurnal Ilmu Pertanian Vol 11, No 1 (2026): April
Publisher : Faculty of Agriculture, Universitas Gadjah Mada jointly with PISPI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ipas.111550

Acid-dry land is a suboptimal land area widely distributed in Indonesia, which has the potential to be developed as cropland to increase crop productivity. However, the low phosphorus availability in this land type often constrains plant growth, necessitating the use of microbial phosphate-solubilizing agents. To address this, the capability of the isolated rhizobacteria strain in phosphate solubilization was improved through a mutagenesis by Ethyl Methane Sulphonate (EMS). Three isolates of indigenous wild-type rhizobacteria of acid-dry land were isolated from cardamom, lemon grass, and bamboo rhizosphere. Mutagenesis of these wild-types yielded three mutant isolates, including KPG_m, SRH_m, and BMB_m, which exhibited higher Phosphate-Solubilization Indices (PSI) compared to their parental strains. The PSI values for the mutants were 2.2, 3.4, and 2.0, respectively, showing a marked improvement over the wild-type values of 2.0, 1.75, and 1.25. These results indicate an enhancement in phosphate-solubilizing ability driven by EMS mutagenesis. All the isolates were then characterized and showed similar traits to those of the Genus of Bacillus. According to the hypersensitive reaction assay, the six isolates showed a negative reaction, indicating they are non-pathogenic. Based on these findings, the mutants have the potential to be developed as biofertilizer agents due to they are non-pathogenic for plants. In addition, the mutants have a moderate PSI value (2<PSI<4) and effective solubilization rate that may improve phosphate solubilization process in acid-dry land.

Marker validation for salt tolerance in Indica rice SUPRAYOGI SUPRAYOGI; PRITA SARI DEWI; EKA OKTAVIANI; ALWA WIDI AISYA
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230918

Abstract. Suprayogi, Dewi PS, Oktaviani, E, Aisya AW. 2022. Marker validation for salt tolerance in Indica rice. Biodiversitas 23: 4517-4523. The development of salt-tolerantrice variety is of importance with concern to the use of the coastal area for rice production and, consequently, the development ofarapid and accurate method of variety selection. Molecular markers associated with Quantitative Trait Loci (QTL) for salt tolerance in rice have been reported elsewhere. This research aimed to identify molecular markers associated with salt tolerance in rice. The research was carried out from September 2020 to February 2021 at Plant Breeding and Biotechnology Laboratory, Faculty of Agriculture, Jenderal Soedirman University, Indonesia. Nine Simple Sequence Repeat (SSR) markers and one Sequence Tagged Site (STS) marker were evaluated for their association with salt tolerance. The rice varieties consisted of three salt-tolerant varieties, one salt-sensitive variety, and six commercial varieties. Nona Bokra PCR amplicon was used as a positive control for DNA band scoring. Binary data of the amplicon banding pattern were analyzed using Principal Component Analysis (PCA) to determine the grouping of the evaluated varieties about their tolerance to assigned markers. The result demonstrated that the evaluated rice varieties fell into four groups of marker resemblances. Among the evaluated SSR markers, RM 3412, RM 342, RM 8094, and Wn 11463 significantly contributed to the grouping. The present study confirmed that Wn 11463 is a strong marker for salt tolerance in rice. However, validation needs to be made on a broader range of genotypes to see the significance of RM 8094 and RM 3412 as salt tolerant markers.

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