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All Journal e-Journal Pustaka Kesehatan Dentika Dental Journal Jurnal Ilmu Kesehatan Masyarakat STOMATOGNATIC- Jurnal Kedokteran Gigi Majalah Kedokteran Gigi Indonesia Jurnal Kesehatan Gigi UNEJ e-Proceeding Dental Journal (Majalah Kedokteran Gigi) Jurnal Kedokteran Gigi Universitas Padjadjaran Padjadjaran Journal of Dentistry Health Notions Meditory : The Journal of Medical Laboratory Jurnal Pengabdian Nasional (JPN) Indonesia
Zahara Meilawaty
Department Of Biomedical, Faculty Of Dentistry, Universitas Jember, Jember, East Java
Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Library & Information Science Medicine & Pharmacology Nursing Public Health Social Sciences Other

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BIOVIABILITY OF BIDURI LEAF EXTRACT (Calotropis Gigantea L.) ON FIBROBLAST CELL CULTURE Sari Setyaningsih; Pudji Astuti; Zahara Meilawaty; Agustin Wulan Suci Dharmayanti
Meditory : The Journal of Medical Laboratory Vol 13, No 1 (2025): Meditory, Volume 13 No. 1 Tahun 2025
Publisher : Jurusan Teknologi Laboratorium Medis, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33992/meditory.v13i1.4049

Abstract

Background: Biduri is recognized for its medicinal properties, particularly in wound healing. The active compounds influence the number of fibroblast cells, which play a role in wound healing. Research on the bioviability of biduri leaf extract has never been conducted, so in vitro testing is necessary to determine the ability of biduri leaf extract to influence fibroblast cells.Objective:  To assess the effect of Biduri leaf extract on the viability of BHK-21 fibroblast cells in vitro. Methods: This experiment used a posttest-only group Design with an MTT assay to assess the viability of BHK-21 fibroblast cells after exposure to different concentrations of Biduri leaf extract. The study employed four treatment groups with varying extract concentrations and used one-way ANOVA followed by an LSD test to analyze the proportion of living cells statistically.Results:  The viability percentages of fibroblast cells varied with the concentration of Biduri leaf extract. The highest viability was observed at 5% concentration (80.82%), while the lowest was at 20% concentration (60.25%). A one-way ANOVA test was used to analyze the data, and differences were considered statistically significant at p 0.05..Conclusions: The highest viability was observed at 5%, while the lowest was at 20% concentration. All treatment groups had cell viability above 60%, indicating that the Biduri extract is generally biocompatible with fibroblast cell culture.
Uji aktivitas antibakteri dan cemaran mikroba daun biduri (Calotropis gigantea l.) sesuai standar mutu bahan baku obat herbal: studi eksperimental laboratoris Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci; Ratna Dewanti, I Dewa Ayu; Yunianti, Ervisya Nandya
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.58528

Abstract

Pendahuluan. Daun biduri (Calotropis gigantea) digunakan oleh masyarakat sebagai obat tradisional khususnya mengobati sakit gigi. Ekstrak daun biduri diketahui memiliki kandungan senyawa flavonoid, triterpene glycosides, abrin, dan alkaloids. Salah satu syarat untuk menjadikan tanaman obat sebagai sediaan farmasi diperlukan standarisasi cemaran mikroba dan uji antibakteri. Tujuan penelitian ini untuk menganalisis aktivitas antibakteri dan cemaran mikroba daun biduri. Metode: Uji cemaran mikroba menggunakan metode angka lempeng total bakteri dan kapang. Sedangkan uji aktivitas antibakteri menggunakan metode difusi cakram dengan konsentrasi ekstrak daun biduri 15%, 20%, 25% dan 30% terhadap bakteri Streptococcus mutans dan Porphyromonas gingivalis. Data  untuk uji aktivitas antibakteri dianalisis menggunakan Anova One Way sedangkan untuk uji cemaran mikroba menggunakan metode deskriptif kuantitatif dibandingkan dengan standar cemaran mikroba berdasarkan Peraturan Kepala Bidang Pengawas Obat dan Makanan (BPOM). Hasil: Uji antibakteri terhadap S. mutans menunjukkan hasil zona hambat kategori sedang, dengan rata-rata diameter zona hambat dari kontrol negatif (0 mm), konsentrasi 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), dan kontrol positif (22,43 mm). Sedangkan pada P. gingivalis tidak ada zona hambat. Hasil uji statistik didapatkan nilai signifikansi p=0,00 (p<0,05). Hasil uji cemaran mikroba menunjukkan hasil perhitungan jumlah bakteri 13.300 cfu/gram dan tidak ditemukan pertumbuhan kapang. Simpulan: Ekstrak daun biduri memiliki daya hambat terhadap Streptococcus mutans, tetapi tidak memiliki daya hambat terhadap Porphyromonas gingivalis. Cemaran ekstrak daun biduri masih memenuhi syarat batas cemaran sesuai standar bahan baku obat herbal.Antibacterial Activity Test and Microbial Contamination of Biduri Leaves (Calotropis gigantea L.) according to Quality Standards for Herbal Medicine: Experimental Laboratory StudiIntroduction. Biduri leaves (Calotropis gigantea) are used by the community as traditional medicine, especially to treat toothache. Biduri leaf extract is known to contain flavonoid, triterpene glycosides, abrin, and alkaloids. One of the requirements for making medicinal plants as pharmaceutical preparations requires standardization of microbial contamination and antibacterial test. Objective: to analyze the antibacterial activity and microbial contamination of biduri leaves. Method: The microbial contamination test uses the total bacterial and fungal plate count method. While the antibacterial activity test uses the disc diffusion method on 15%, 20%, 25% and 30% biduri leaf extract against Streptococcus mutans and Porphyromonas gingivalis. Data for antibacterial activity test were analyzed using Anova One Way while for microbial contamination test using quantitative descriptive method and compared with microbial contamination in accordance with Regulation of Head of Drug and Food Supervisory Division (BPOM). Results: Antibacterial test against S. mutans showed moderate inhibition zone result, with average diameter of inhibition zone from negative control (0 mm), concentration 15% (8,94 mm), 20% (9,05 mm), 25% (9,65 mm), 30% (9,79 mm), and positive control (22,43 mm). Meanwhile, in P. gingivalis there is no inhibition zone. Statistical test result obtained significance value p=0.00 (p<0.05). Microbial contamination test result showed bacterial count result 13,300 cfu/gram and no mold growth was found. Conclusion: Biduri leaf extract has inhibition against S. mutans, but has no inhibition against P. gingivalis. The contamination of Biduri leaf extract still meets the contamination limit according to the standard of herbal medicinal raw materials.
Uji efektivitas antibakteri daun Biduri (Calotropis gigantea) terhadap pertumbuhan Streptococcus mutans: studi eksperimental laboratoris Widodo, Alaya Dwi Salvahira; Setyaningsih, Sari; Astuti, Pudji; Meilawaty, Zahara; Suci Dharmayanti, Agustin Wulan
Jurnal Kedokteran Gigi Universitas Padjadjaran Vol 36, No 3 (2024): Desember 2024
Publisher : Fakultas Kedokteran Gigi Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/jkg.v36i3.56148

Abstract

Pendahuluan: Karies merupakan penyakit jaringan keras pada gigi yang disebabkan oleh asam hasil bakteri plak yang menumpuk pada permukaan gigi. Salah satu bakteri plak adalah Streptococcus mutans. Penggunaan obat kumur Chlorhexidine gluconate 0,2% terbukti mampu menghambat S.mutans. Namun penggunaan Chlorhexidine gluconate 0,2% dalam jangka panjang dapat menimbulkan efek samping. Oleh karena itu, diperlukan bahan alami seperti tanaman biduri yang memiliki efek antibakteri untuk menghindari efek samping tersebut. Penelitian ini bertujuan menganalisis ekstrak daun biduri (Calotropis gigantea) dapat menghambat pertumbuhan S.mutans. Metode: Jenis penelitian eksperimental laboratorium. Penelitian ini menggunakan 6 kelompok perlakuan yaitu kelompok perlakuan ekstrak daun biduri dengan konsentrasi 15%, 20%, 25%, 30%, K(-) akuades, dan K(+)Chlorhexidine gluconate 0,2%. Kelompok perlakuan dan kelompok kontrol dipaparkan S.mutans untuk melihat efek antibakterinya dan menggunakan metode spektrofotometri UV-Vis dan hitung koloni bakteri (total plate count). Hasil: Hasil penelitian menunjukkan, masing-masing konsentrasi menunjukkan hasil laju penghambatan sebesar 0 pada kelompok K(-), 45,78% pada kelompok 15%, 34,93% pada kelompok 20%, 76,5% pada kelompok 25%, 64,45% pada kelompok 30%, dan 89,45% pada kelompok K(+). Hasil  hitung koloni bakteri penghambatan bakteri terbesar yaitu pada konsentrasi 25%. Hasil yang diperoleh dilakukan analisis data. Data sebelum dan sesudah inkubasi dilakukan uji Paired T Test dan menunjukkan perbedaan yang signifikan. Analisis data laju penghambatan dilakukan dengan uji Kruskal Wallis dilanjutkan uji Mann Whitney Ekstrak daun biduri konsentrasi 15% dibandingkan dengan konsentrasi 20% dan 30% dibandingkan dengan 25% memiliki nilai p>0,05 sehingga tidak memiliki perbedaan yang bermakna. Simpulan: Daun biduri efektif menghambat pertumbuhan S.mutans.Antibacterial effectiveness test of biduri leaf (Calotropis gigantea) against Streptococcus mutans: experimental laboratory studyIntroduction: Caries is a disease that affects the hard tissue of the teeth, caused by acid deposits produced by plaque bacteria that accumulate on the tooth surface. One of the plaque bacteria is Streptococcus mutans. Chlorhexidine gluconate 0.2% mouthwash has been shown to inhibit S. mutans. However, prolonged use of Chlorhexidine gluconate 0.2% mouthwash can cause side effects. To avoid these side effects, natural ingredients like biduri plant that have antibacterial effects are needed. This research aims to analyze the inhibitory effects of biduri (Calotropis gigantea) leaf extract on the growth of S. mutans. Methods: This research is experimental laboratory study. Six treatment groups were used, four groups treated with biduri leaf extract at concentrations of 15%, 20%, 25%, 30%; the K(-) using distilled water,; and K(+) using Chlorhexidine gluconate 0.2%. The treatment and control group were exposed to S. mutans to evaluate its antibacterial effect and assessed using the UV-Vis spectrophotometric and total plate count method. Results: In the research conducted, each concentration showed varying inhibition rates. The inhibition rates observed were as follows: 0% for the K(-), 45,78% for the 15% group, 34,93% for the 20% group, 76,5% for the 25% group, 64,45% for the 30% group, and 89,45% for the K(+). In bacterial colony count, the greatest bacterial inhibition was observed at concentration of 25%. Statistical analysis was performed on the obtained data. A Paired T-Test demonstrated a significant difference between the data before and after incubation. Further analysis using the Kruskal Wallis and Mann Whitney tests revealed no significant difference in inhibition rates between 15% and 25% concentrations, or between 25% and 30%. Conclusion: Biduri leaf extract effectively inhibits the growth of S. mutans. 
Examining the Impact of Porphyromonas gingivalis Infection on Ovarian Health: A Reduction in Primordial Follicles in a Female Periodontitis Model Meilawaty, Zahara; Galau, Argha Sirna; Permatasari, Sabania Dinar; Setyaningsih, Sari; Astuti, Pudji; Setiawatie, Ernie Maduratna; Hendarto, Hendy; Suci Dharmayanti, Agustin Wulan
Jurnal Kesehatan Gigi Vol 12, No 1 (2025): Juni 2025
Publisher : Jurusan Kesehatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v12i1.12547

Abstract

Periodontitis is a chronic inflammation of the periodontal tissues caused by periodontal pathogens, such as Porphyromonas gingivalis (P. gingivalis). P. gingivalis possesses several virulence factors that enable it to invade and spread through the bloodstream to other organs, including the ovaries. It triggers inflammation, which may impact the number of primordial follicles. The aim of this study was to assess the effects of P. gingivalis infection on the number of primordial follicles in the ovaries. This study was a laboratory experiment with a post-test control group design. The rats were divided into control and periodontitis groups. The periodontitis group consisted of rats induced with P. gingivalis in their sulcus gingiva of the first mandible molar every three days for 19 days. Therefore, control groups were without P. gingivalis induction. Seven days after the last induction of P. gingivalis, the rats were euthanized, and their ovarian tissues were collected for histological preparation. The primordial follicles were counted under 400x magnification. The results indicated that the treatment group had a significantly lower number of primordial follicles (2.2 ± 2.61) compared to the control group (12.4 ± 2.41). The study concludes that P. gingivalis infection led to a reduction in the number of ovarian primordial follicles in periodontitis models.
The effect of cassava (Manihot esculenta) leaf extract on COX-2 expression in the neutrophil cell culture exposed to the lipopolysaccharide of Escherichia coli (in-vitro study) Meilawaty, Zahara; Dharmayanti, Agustin Wulan Suci; Prafitasari, Dinar
Padjadjaran Journal of Dentistry Vol 31, No 1 (2019): March 2019
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (271.311 KB) | DOI: 10.24198/pjd.vol31no1.16950

Abstract

Introduction: Neutrophils are cells which played an initial role in the inflammation area and can be induced by lipopolysaccharide (LPS). Activated neutrophils will secrete the COX-2 enzyme which is involved in arachidonic acid synthesis to produce inflammatory mediators. However, excessive inflammation can cause tissue destruction; thus, the body needs anti-inflammation agents. Cassava leaf contains biochemical component which has an anti-inflammation effect. This study was aimed to determine the expression of COX-2 in neutrophils administered with cassava leaf extract and the LPS of E. coli. Methods: This study was experimental laboratories (in vitro study) using human neutrophil cells culture. The samples of this study were divided into 4 groups, administered with 12.5% cassava leaf extract (T1); 25% cassava leaf extract (T2); control group which contained only neutrophils cells (C1); and control group contained neutrophils and LPS (C2). Human neutrophil cells culture was incubated in the 12.5% and 25% cassava leaf extract before exposed with the LPS of E. coli. COX-2 expression was observed with immunochemistry methods. Results: The COX-2 expression in the 12.5% cassava leaf extract group was significantly higher than the 25% cassava leaf extract group (p < 0.05). Conclusion: Cassava leaf extract can inhibit the COX-2 expression and can be used as an alternative anti-inflammation agent.Keywords: Anti-inflammation, cassava leaf extract, COX-2 expression, neutrophils.
The potential of cassava leaves extract in gingival fibroblasts of periodontitis of rats with ovarian dysfunction Shita, Amandia Dewi Permana; Meilawaty, Zahara; Rothschildi, Dhesyarmani Putri; Dharmayanti, Agustin Wulan Suci; Hamzah, Zahreni
Padjadjaran Journal of Dentistry Vol 33, No 3 (2021): November
Publisher : Faculty of Dentistry Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/pjd.vol33no3.29611

Abstract

Introduction: The prevalence of periodontitis has reached 42.8% in Indonesia. One contributing factor is Porphyromonas gingivalis. The ovarian dysfunction caused by ovariectomy procedures may also cause periodontitis. Post ovariectomy conditions resemble menopause, lowering the production of estrogen and progesterone hormones, leaving patients vulnerable to periodontitis. To prevent the side effects of the treatment, herbal ingredients are commonly used. The extract of cassava leaves is known to have pharmacological effects as an antioxidant and, thus, can be applied as a medicine for periodontitis.      Methods: A total of 21 rats were divided into three groups, (1) control: healthy rats; (2) Group P.1: P. gingivalis-induced rats; (3) Group P.2: ovariectomized rats. Groups P.1 and P.2 were further divided into three subgroups that were administered cassava leaves extract at a dosage of 179.2 mg/ kg BW, aquades and vitamin C. Rats were euthanized at day eight after the initial treatment to collect left mandibular first molar. The mandibular sections were immunohistochemically stained to quantify SOD expression using light microscope while the Image J software was used. Fibroblast cells expressing SOD are characterized by brown coloration in the cytoplasm and darker nucleus. Results: In periodontitis conditions, either due to P.gingivalis induction or ovarian dysfunction, fibroblast cells in rats given cassava leaves extract expressed higher SOD than rats given aquades, but still lower than rats given vitamin C. The result of the one way ANOVA test showed p value of 0,00, which means that there is a significant difference in all groups. Conclusion: Cassava leaves extract demonstrated the potential to increase the antioxidant expression in P. gingivalis-induced and ovarian dysfunction rats.
Co-Authors Agustin Wulan Suci Dharmayanti Alisah, Putri Alisah Amandia Dewi Permana Shita Amandia Dewi Permana Shita Amelia Nur Ilahi Ari Tri Wanodyo Handayani Banun Kusumawardani Budi Prabowo Budi Prabowo Desi Sandra Sari Desi Sandrasari Dessy Rachmawati Dhea Ayu Dewanti Dyah Indartin Dyah Indartin Setyowati Ernie Maduratna Setiawatie Galau, Argha Sirna Galdhisia Devita Caresya Happy Harmono Hendy Hendarto Hestieyonini Hadnyanawati Kholidiya, Wulan Ratna Nur Kiswaluyo . Lutfi Meiga Sari M. Nurul Amin Muhammad Nurul Amin Nabilla, Purry Roro Nulend, Jenneke Klein Octaviana Putri Puspita Sari Oktavia Nur Vitasari Paramudibta Lungit Kuncaraningtyas Permatasari, Sabania Dinar Pradnyani, Ni Luh Kadek Ardhia Swari Prafitasari, Dinar Pudji Astuti PUDJI ASTUTI Ratna Dewanti, I Dewa Ayu Rendra Chriestedy Prasetya Rido Tri Andika Firdyansyach Ristya Widi Endah Yani Rothschildi, Dhesyarmani Putri Sari Setyaningsih Tantin Ermawati Tecky Indriana Widodo, Alaya Dwi Salvahira Yunianti, Ervisya Nandya Zahreni Hamzah Ziaulhaq, Mochamad Najib