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All Journal Pharmaciana: Jurnal Kefarmasian Jurnal Kedokteran Indonesia Omega: Jurnal Fisika dan Pendidikan Fisika JURNAL FARMASI DAN ILMU KEFARMASIAN INDONESIA Panrita Abdi - Jurnal Pengabdian pada Masyarakat Jurnal Farmasi Sains dan Komunitas Health Notions Warta Pengabdian JFIOnline Pharma Xplore : Jurnal Sains dan Ilmu Farmasi Jurnal Abdi: Media Pengabdian Kepada Masyarakat Jurnal Layanan Masyarakat (Journal of Public Service) Berkala Ilmiah Kimia Farmasi Berkala Penelitian Hayati Journal of Tropical Pharmacy and Chemistry
Isnaeni
Departemen Ilmu Kefarmasian Fakultas Farmasi Universitas Airlangga, Indonesia
Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Chemistry Decision Sciences, Operations Research & Management Dentistry Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Journal : Berkala Penelitian Hayati

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Antimicrobial activity of Streptomyces spp. isolates from vegetable plantation soil Isnaeni Isnaeni; Idha Kusumawati; Mega Ferdina Suwito; Asri Darmawati; Ni Made Mertaniasih
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 21 No 2 (2016): June 2016
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (639.33 KB) | DOI: 10.23869/78

Abstract

Fifteen Streptomyces isolates were isolated from soil in some different location on vegetable plantation at agriculture standard condition. The isolates were assessed for their antibacterial activity against Mycobacterium tuberculosis (MTB) ATCC H37RV and mycobacterial which isolated from Dr. Soetomo Hospital patients in Surabaya. The Interna-tional Streptomyces Project 4 (ISP4) and Middlebrook 7H9 (MB7H9) wwere used as growth or fermentation medium. The screening of inhibition activity was performed using turbidimetry and spot-test on agar medium. Results shown that 33.3% of the isolates (5 isolates) have anti-mycobacterial activities. The first line anti tuberculosis drug rifampicin, (RIF), ethambutol (EMB), isoniazid (INH), and pyrazinamide (PZA) were used as standards or positive controls with concentration 20 ppm. Optical density of crude fermentation broth concentrated from five isolates relatively lower than five anti-tuberculosis drug activity standard, although their activities against some microbial were similar to the stand-ard at spot-test. The most efficient isolate shown anti-mycobacterial activity was Streptomyces B10 which identified as Streptomyces violaceousniger. In addition, fatty acid methyl ester (FAME) profile of gas chromatography-mass spec-trometry chromatogram of each isolates were studied and compared to Streptomyces spp.
KAJIAN PENDAHULUAN UJI TOKSISITAS EKSTRAK AIR MISELIA DAN TUBUH BUAH JAMUR SHIITAKE (Lentinus edodes) DENGAN METODE BRINE SHRIMP LETHALITY TEST (BST) Noor Erma NS; Tri Sundari; Arie Ika Susanty; Dwi Riani Oktavia Palupi; Isnaeni; Sukardiman
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 10 No 1 (2004): December 2004
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/372

Abstract

Shiitake mushroom (Lentinus edodes) is one of the wood mushroom types that can be consumed as a food as well as for a medical purpose. Lentinan, a polysaccharide contained in shiitake, is well known for its use on cancer medication. Mycelium of Shiitake mushroom contains lentinan the same as other part of the mushroom like fruity body. Toxicity of the lentinan in mycelium compare to the fruity body has been first conducted by using Brine Shrimp Lethality Test (BST). Using Potato Dextrose Broth media with the growth rate of 3.88% did mycelium multiplications. Probit analysis showed that the toxicity of the mushroom’s cap, stem, and mycelium of Shiitake mushrooms is LC50 = 648.76507 mg/ml LC50 = 489.39444 mg/ml, and LC50 = 481.16941 mg/ml respectively.
Inhibitory activity of fermentation filtrate of red passion fruit pulp (Passiflora edulis sims.) against Escherichia coli extended-spectrum beta-lactamase (ESBL) and methicillin resistant Staphylococcus aureus (MRSA) Iif Hanifa Nurrosyidah; Ni Made Mertaniasih; Isnaeni
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 26 No 1 (2020): December 2020
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23869/bphjbr.26.1.20204

Abstract

This study aims to determine the minimum inhibitory concentration (MIC) and bactericidal concentration (MBC) of the fermentation filtrate (FF) on red passion fruit pulp (Passiflora edulis Sims.) against Escherichia coli Extended Spectrum Beta Lactamase (ESBL) and Methicillin Resistant Staphylococcus aureus (MRSA). The method used was the fermentation of red passion fruit pulp for 24 h using De Man Rogosa Sharpe Broth (MRS-broth) media, and the result showed that the FF of red passion fruit pulp made a dilution series concentration of 50%, 25%, 12.5%, 6.25%. Considering this result, the MIC FF of red passion fruit cells against ESBL and MRSA has a 25% dilution concentration, while MBC has a 50% dilution concentration. This shows that red passion fruit has the potential to be developed as antibacterial material, especially to fight bacteria that are already resistant.
Co-Authors A'yun, Arini Qurrata Achmad Toto Poernomo Aditya Fridayanti Aditya Fridayanti Aditya Fridayanti Alief Putriana Rahman Alimsardjono, Lindawati Ambarini Indah Andri Astuti Andyanita Hanif Hermawati Anggita Mirzautika Aprelita Nurelli Dwiana Arie Ika Susanty Aryati Aryati Asri Darmawati Astrid Aulia Hamida Dahlang Tahir Diyah, Nuzul Wahyuning Djoko Agus Purwanto Dwi Riani Oktavia Palupi Esti Handayani Esti Hendradi Febri Annuryanti Fridayanti, Aditya Hadi Poerwono Hana Sofiana Maghfira Hanifah Ridha Rabbani Hepriyadi, Selvy Uftovia I Gede Edy Sagitha Idha Kusumawati Ifah Yulistyani Izza Rahmi Hidayah Juni Ekowati Kholis Amalia Nofianti Laili Irfanah Lisa Nathalie Luky Hayuning Les Mahfudz Mahfudz Marcellino Rudyanto Mega Ferdina Suwito Meliana Susanti Muhammad Agus Syamsur Rijal Muhammad Muslich Muhammad Mu’amar Fathoni Muhammad Yuwono Muhammad Yuwono Muhammad Yuwono Muhammad yuwono Nailatul Hidayah Ni Made Mertaniasih Nindya Pramesti Wardani Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Noor Erma Nasution Sugijanto Noor Erma NS Nuril Fikriyah Nurrosyidah, Iif Hanifa Primadi Avianto Pujianti, Efrin Pujianti, Efrin Putri, Radha Hartina Ramlan, Nurul Mutmainnah Riesta Primaharinastiti Rifaatul Mahmudah Siswandono, Siswandono Sonia Khoirun Nisa Sri Poedjiarti Sudjarwo Sudjarwo Sugijanto Sugijanto Sugijanto Randiman Sugiyartono, . Sugiyartono, . Suharjono Suharjono Suharjono, Suharjono Sukardiman Suko Hardjono Susanti Susanti Suzana Syahrani, Achmad Syamsul Arifin Tri Sundari Utari Ardiningdyah Widji Soeratri Yulistiani Yulistiani Zahratus Silmi Aliyah