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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indonesian Journal of Dental Medicine
Tamara Yuanita
Conservative Dentistry Department, Faculty Of Dentistry, Universitas Airlangga,
Dentistry

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Apexification treatment on tooth with a history of trauma: A case report Juniarti, Devi Eka; Anandita, Tiara; Tania, Cindy Grace; Prasetyo, Eric Priyo; Yuanita, Tamara
Conservative Dentistry Journal Vol. 14 No. 1 (2024): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i1.2024.15-18

Abstract

Background: Traumatic dental injuries to the teeth may result in pulpal and periapical disease. Most dental traumas occur in the 7 -10-year-old age group with incomplete apical root development. Apexification is the procedure to close the apex using bioactive material to create an apical plug. MTA can be used as a material of choice to treat open apex. Purpose: To perform an apexification of open apex with MTA apical plug. Case: A 30-year-old female patient with a chief complaint to fix her missing anterior filling and blackened tooth. The tooth had a history of trauma when the patient was 7 years old. One year ago, the tooth had intermittent pain so the tooth was treated and filled by another dentist. Case Management: Access opening is performed and apical gauging is determined. Then, working length was established. Debridement was done with H-file #80. Irrigation sequence with NaOCl 1,5% - Aquadest – EDTA 17% -aquadest. Ca(OH)2 dressing was given and temporary restoration was placed. On the second visit, temporary restoration was removed, followed by a root canal filled with MTA 3 mm from the apex using MAP carrier then confirmed with a radiograph. Conclusion: Trauma that occurs during tooth formation may cause an open apex that is treatable using an MTA plug.
Comparison of ratios combination calcium hydroxide Ca(OH)2 with red pine (Pinus densiflora) of viscosity Wahjuningrum, Dian Agustin; Mooduto, Latief; Subiyanto, Ari; Prasita, Shafa; Yuanita, Tamara
Indonesian Journal of Dental Medicine Vol. 4 No. 1 (2021): Indonesian Journal of Dental Medicine
Publisher : Faculty of Dental Medicine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ijdm.v4i1.2021.26-31

Abstract

Background: Calcium hydroxide is a gold standard for root canal sealing material. However, there are many defects in calcium hydroxide that have prompted many researchers to look for alternative materials, including natural origin ingredients. Red pine (Pinus densiflora) is often used in dentistry for its antioxidant and antibacterial properties. The mixture of calcium hydroxide and red pine has never been achieved before. One of the criteria for material sealing is that the channel should be fully sealed with high flow ability and low viscosity. Therefore, this research is performed to analyze the viscosity value of the mixture of calcium hydroxide and red pine (Pinus densiflora). Purpose: Knowing the difference in viscosity of the combination of calcium hydroxide and red pine with a ratio of 1:1, 1:1.5, and 1:2. Methods: This study used four treatment groups, with each group consisting of 8 replications. Group 1 was a combination of calcium hydroxide and red pine with a 1: 1, group 2 the ratio was 1: 1.5, group 3 had the ratio 1: 2, and the positive control group used calcium hydroxide and a sterile aquadest. Calcium hydroxide in powder form and an extract of red pine in liquid form are mixed according to the ratio. The viscosity value is measured using a viscosity tester, namely the Brookfield Viscometer. Results: The control group had a lower viscosity than group 3, group 3, and group 2 than group1. Conclusion: Combination of calcium hydroxide and red pine with a ratio of 1:2, the lowest viscosity was obtained compared to the ratio of 1: 1 and 1: 1.5.
A review of sodium diclofenac as a potential medicament to eliminate Enterococcus faecalis in regenerative endodontic treatment Prasetyo, Eric Priyo; Yuanita, Tamara; Setyabudi; Bin Sharizal, Shafy Shariz; Sukandar, Wilson; Paidal, Nurfahira; Charles, William; Christabel, Phebe Fedora; Liong, Michelle; Prasetyaningtias, Silvia Arlita Hayu
Conservative Dentistry Journal Vol. 14 No. 2 (2024): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v14i2.2024.71-74

Abstract

Background: Traumatic dental injuries often occur in the community reaching 85% of patients and in preschool children the proportion reaches 17%. Among the injuries are tooth fracture, tooth luxation, tooth avulsion and open apex which causes pulp death or necrosis, causing root growth to stop. Regenerative endodontic treatment (RET) with a biological approach to treat necrotic immature permanent teeth can promote pulpal growth, increase the thickness of the dentin wall and form apical foramens. Enterococcus faecalis bacteria can be resistant to some drugs and root canal irrigation substances and may persist in the root canal system. Recently, sodium diclofenac has been reported to retain a new potential to eliminate Enterococcus faecalis biofilm. This shows that sodium diclofenac is potential as an anti-biofilm material. Purpose: To evaluate the potential use of sodium diclofenac in RET. Review: This review was investigated in PubMed and Google Scholar containing keywords: Sodium Diclofenac, Enterococcus faecalis and Regenerative Endodontic Treatment. Sodium diclofenac is a potential medicament to eliminate Enterococcus faecalis biofilm therefore has a potency to be used in Regenerative Endodontic Treatment. Conclusion: The success rate of RET depends on the elimination of microorganisms in the root canal systems. The use of sodium diclofenac as a medicament to eliminate Enterococcus faecalis biofilm is potential to be used in RET. Further researches are encouraged to explore the benefits of sodium diclofenac as a medicament in RET.
Effect of Butterfly Pea Flower (Clitoria ternatea L.) Kombucha Against Streptococcus viridans Yuanita, Tamara; Firmansyah, Awang Bagus; Ulfadi , Bakhiitah Thufailah Putri; Prasetyo, Eric Priyo; Wahjuningrum, Dian Agustin
Conservative Dentistry Journal Vol. 15 No. 1 (2025): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i1.2025.14-17

Abstract

Background: Dental caries is a multifactorial disease driven by the formation of bacterial biofilms, particularly Streptococcus viridans, which contribute to root canal infections if not appropriately managed. Sodium hypochlorite (NaOCl) is commonly used as an irrigant in root canal therapy, but its application is limited due to its toxicity and corrosiveness. Consequently, there is a pressing need for safer and more effective natural alternatives. Kombucha derived from butterfly pea flower (Clitoria ternatea L.) has been identified as a promising candidate with antibacterial and antibiofilm properties due to its bioactive secondary metabolites. The fermentation process involving a symbiotic culture of bacteria and yeast (SCOBY) may further enhance the efficacy of these bioactive compounds. Purpose: This study conducted to analyze the effect of kombucha from butterfly pea flower (Clitoria ternatea L.) on the biofilm of Streptococcus viridans in vitro, utilizing a spectrophotometric method to assess the impact across various concentrations. Methods: An experimental laboratory study was conducted in vitro employing a post-test-only control group design. Kombucha prepared from butterfly pea flower was fermented for periods ranging from 12 to 154 days, followed by dilution into several concentrations (100%, 50%, 25%, 12.5%, 6.25%, 3.12%, and 1.56%) using the dilution method. Direct contact between the kombucha and Streptococcus viridans was established, and the resulting biofilm inhibition was assessed by measuring the Optical Density (OD) using a spectrophotometer at a wavelength of 650 nm. Results: The inhibitory percentage of butterfly pea flower kombucha against Streptococcus viridans biofilm decreased progressively with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%. The highest inhibitory percentage was observed at a concentration of 100%. Conclusion: Kombucha of butterfly pea flower (Clitoria ternatea L.) demonstrated potential in inhibiting Streptococcus viridans biofilm formation, with a concentration of 25% determined as the MBIC50. However, an MBEC90 value could not be established, as no biofilm inhibition percentage exceeding 90% was observed in the test results.
Effect of kombucha butterfly pea (Clitoria ternatea) solution on Enterococcus faecalis biofilm Yuanita, Tamara; Marpaung, Grace Julieta; Wiratno, Amelia Rahma; Wahjuningrum, Dian Agustin; Juniarti, Devi Eka
Conservative Dentistry Journal Vol. 15 No. 2 (2025): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v15i2.2025.62-67

Abstract

Background: Enterococcus faecalis is a facultative anaerobic bacterium that is often found in the root canals of teeth with pulp necrosis and is often the cause of endodontic treatment failure. On the other hand, the fermented beverage kombucha is made using a fermentation process by bacteria and yeast, known as SCOBY (Symbiotic Culture of Bacterial and Yeast). The fermentation process in kombucha causes a decrease in the pH of the medium, where the bacteria in the SCOBY convert sugar into ethanol and acetic acid. The butterfly pea flower (Clitoria ternatea) has single petals of purple, blue, pink, or white color and is proven to have antibacterial as well as antibiofilm properties. Purpose: To determine the effect of kombucha butterfly pea (Clitoria ternatea) solution on Enterococcus faecalis biofilm. Methods: Kombucha butterfly pea with concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%, as well as NaOCl 2,5% was given Enterococcus faecalis biofilm. To determine biofilm formation, test tubes were washed, adherent cells were stained with 0.1% crystal violet, and light absorbance was measured with a spectrophotometer at a wavelength of 540nm. Results: The percentage of inhibitory power of kombucha butterfly pea against E. faecalis biofilm decreased gradually from concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, and 1.56%. The highest percentage of inhibition was in kombucha with butterfly pea with a concentration of 100% which is equivalent to 2,5% NaOCl as an antibiotic for root canal irigation. Conclusion: Kombucha of butterfly pea flower (Clitoria ternatea) can reduce the decrease in Enterococcus faecalis biofilm formation with a concentration of 12.5% designated as MBIC50 and a concentration of 100% designated as MBEC90.
Co-Authors Adioro Soetojo Aditya Rama Devara Afifah, Anisa Nur Agus Subiwahjudi Agus Subiwahjudi Agus Subiyanto Alissa Amanda Amalia Iqony, Rosa Anandita, Tiara Ari Subiyanto Ari Subiyanto, Ari Arindah Hadi Bin Sharizal, Shafy Shariz Cecilia G.J Lunardhi Cecilia lunardhi Chairil Hafiz, Teuku Charles, William Christabel, Phebe Fedora Deavita Dinari Devi Eka Juniarti Dian Agustin Wahjuningrum Dina Ristyawati Edhie Arief Prasetyo Edhie Arif Pasetyo Edhie Arif Prasetyo Eric Priyo Prasetyo Fajar Agus Muttaqin Fajariana Fitriani Firmansyah, Awang Bagus Galih Sampoerno Hadriany Hotmaria Ika Tangdan Jesica Ceren Karlina Samadi Ketut Suardita, Ketut Kuntaman Kuntaman Latief Mooduto, Latief Liong, Michelle M. Mudjiono M. Roelianto Mandojo Rukmo Marbun, Tarsardo Marpaung, Grace Julieta Mega Selvia Mifta Izha A R Mohammad Roelianto Mohammed Alaqsha Brysoul Ceson Nabilla, Tasya Nadien Sekar Pramesti Nanik Zubaidah Nila Murni Wulandari Nirawati Pribadi Paidal, Nurfahira Paramitha, Shafa Marwa Moza Prasetyaningtias, Silvia Arlita Hayu Prasita, Shafa Qintan Sekar Adjani R. Roulianto Randy Carlos Sietho Rega Maurischa Arantika Putri Reinold Christian Lina Revina Ester Iriani Marpaung Richard Fritzgerald Rifatul Jannah Ruslan Effendy Ruslan Effendy Salma, , Adinda Fazzahra Sepdhyo Wahyu Nugroho Setyabudi Setyabudi Setyabudi Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Sylvia Paulina Panggono Tania, Cindy Grace Tantri Wismayaning Radito Tarigan, Shindyloken Juni Artha Tarsardo Marbun Teuku Chairil Hafiz Ulfadi , Bakhiitah Thufailah Putri Ulfadi, Bakhiitah Thufallah Putri Uli Sasi Andari Wiratno, Amelia Rahma Yuliana Dwiwahyu Suryandari Yulianti Kartini Sunur