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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indonesian Journal of Dental Medicine
Tamara Yuanita
Conservative Dentistry Department, Faculty Of Dentistry, Universitas Airlangga,
Dentistry

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The Difference of Antibacterial Power between Cocoa Peel (Theobroma Cacao L.) Extract 6% compared to Chlorhexidine Digluconate 2% Againts Streptococcus mutans (In vitro) Alissa Amanda; Tamara Yuanita; Galih Sampoerno
Conservative Dentistry Journal Vol. 11 No. 1 (2021): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i1.2021.1-5

Abstract

Background: Before restoration, it is necessary to clean the cavity from the smear layer and residual bacteria such as Sreptococcus mutans using a 'gold standard' cavity cleanser, namely 2% Chlorhexidine digluconate (CHX), however CHX 2% has a disadvantage of having a toxic effect on fibroblasts, osteoblasts, myoblasts, odontoblast-like cells, Chinese hamster ovary cells, and buccal epithelial cells. The shortcomings of the 2% CHX triggered researchers to look for alternative cavity cleansers that are more biocompatible, namely cocoa peel extract because it contains of antibacterial compounds including alkaloids, flavonoids, tannins, saponins, and terponoids with a non-toxic 6% concentration. Purpose: To analyze the difference of antibacterial activity between cocoa peel extract with a concentration of 6% compared to chlorhexidine digluconate 2% against Streptococcus mutans. Methods: This research was an in vitro laboratory experimental study with the posttest only control group design which included two treatment groups, namely 6% cocoa peel extract and 2% CHX. This research was conducted using the inhibition zone diffusion method against S. mutans to see the antibacterial power of each sample. Results: There was a significant difference (p <0.05) in the mean diameter of the inhibition zone between 6% cacao peel extract, namely 11.5406 mm and CHX 2%, namely 13.2156 mm.  Conclusion: Chlorhexidine digluconate 2% has a greater antibacterial power than 6% cocoa peel extract (Theobroma cacao L.) against Streptococcus mutans. 
One visit endodontic on asymtomatic mummificated mandibular molar Sholeh Ardjanggi; Dian Agustin Wahjuningrum; Tamara Yuanita; Eric Priyo Prasetyo
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.80-82

Abstract

Background. The main goal of root canal treatment is the prevention or treatment of apical periodontitis, which leads to the preservation of natural teeth. Traditionally, root canal treatment is carried out in a number of visits, using extra disinfecting agents in addition to irrigant used during cleaning and shaping procedures that primarily aim to reduce or eliminate microorganisms and their byproducts. from the root canal system before it is obtained. Recent advances in Endodontic technology, attracting dental experts and endodontists to perform root canal treatment in one visit. Numerous studies evaluate the effectiveness of single-versus multiple-appointment root canal treatment have been published, which reported no significant differences in effectiveness (healing rates) between these two treatment regimens.Purpose : preservation of the integrity of the molar teeth that have symptomatic complaints by taking proper care with a short visit time. Caset: A 21-year-old woman presents with concern in her lower left tooth, the patient tells that the tooth had been treated before at the dentist when she was a child. On clinical examination there is a composite restoration in occlusal tooth # 36, non vital teeth. On periapical radiology there are radiopaque on pulp chamber and a diffuse radiolucent area in the apical area of tooth # 36, then the diagnosis then determined Previously initiated therapy with asymptomatic apical periodontitis. Case Management : Tooth # 36 treated with one visit root canal treatment, Treatment is continued with fiber post installation and crown, patients no longer complain of pain after treatment. Teeth function normally again. Conclusion: One visit root canal treatment can successfully eliminate patient complaints and restore tooth function.
Differences in Effectiveness of Antibacterial Power Between Cocoa Peel Extract (Theobroma cocoa L.) and Benzalkonium Chloride 0.1% Against Staphylococcus aureus (In Vitro) Tamara Yuanita; Mohammed Alaqsha Brysoul Ceson; Agus Subiyanto
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.56-61

Abstract

Background: Staphylococcus aureus is a gram-positive bacteria play a role in the formation of dental biofilm which iscausing dental caries. During tooth preparation, to stop the growth of bacteria, a cavity cleaning agent is given using achemical, namely Benzalkonium Chloride (BAC) 0.1%, but BAC has disadvantages including allergic reactions, tolerantmicrobes, and resistance. Therefore, it is hoped that there will be herbal ingredients that can be used as an alternative.Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which haveantibacterial concentration 6% according to safe concentrations. Purpose: To explain the difference in the effectivenessof the antibacterial power of 6% cocoa peel extract and 0.1% BAC against Staphylococcus aureus (in vitro). Methods:This study was a laboratory experimental in vitro with the posttest only control group design. Using the diffusion methodfor Staphylococcus aureus that divided into two parts, 6% cocoa peel extract and 0.1% BAC. Each petri dish was givendisc paper dripped with 0.01 ml of each test material, then incubated for two days and observed the diameter of theinhibition zone. Results: The average diameter of the inhibition zone formed in the 6% cacao peel extract was 11.5288mm and BAC 0.1% was 18.2925 mm against Staphylococcus aureus. Conclusion: There was a significant difference inthe effectiveness of antibacterial power (p <0.05) between 6% cacao peel extract (Theobroma cacao L.) and 0.1% BACagainst Staphylococcus aureus (In Vitro).
The Difference of Antibacterial Power Between Cocoa Peel (Theobroma cacao) Extract 6% Compared to NaOCL 5% Againts Lactobacillus acidophilus Tamara Yuanita; Dian Agustin Wahjuningrum; Mega Selvia
Conservative Dentistry Journal Vol. 11 No. 2 (2021): July - December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v11i2.2021.67-71

Abstract

Background: Lactobacillus acidophilus is a gram-positive rod-shaped bacteria which mostly causes chronic caries lesions. In the process of cavity preparation it is reported that it is not possible to remove all microorganisms and even bacteria can survive even after removal of the necrotic tissue. Efforts to stop the growth of these bacteria are by chemical cavity cleanser using a 5% NaOCl, but this material have disadvantages including irritating periradicular tissue, unpleasant odor, and toxicity. Due to its disadvantages, it is hoped that natural ingredients can be used as alternatives. Cocoa peel extract has active compounds of tannins, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial ability with a concentration of 6% according to the non-toxic minimum kill concentration of Lactobacillus acidophilus. Purpose: To analyze the difference in antibacterial power between the extract of cocoa bark with a concentration of 6% compared to 5% NaOCl against Lactobacillus acidophilus. Methods: This study was a laboratory experimental in vitro with a post test-only control group design. Using diffusion method against Lactobacillus acidophilus grown in tubes containing BHIB, then cultured in a petridish which contains a nutrient agar and is divided into 2 parts extract cocoa peel extract 6% and NaOCl 5%, then each petridish given a paper disc that has been poured 10μl by each material, then incubated inthe incubator for 2x24 hours at 37oC and observe the diameter of the inhibition zone formed using a caliper. Results: The average diameter of the inhibition zone formed in the 6% cocoa peel extract was 11.8375 mm and NaOCl 5% was 26.0344 mm against Lactobacillus acidophilus. Conclusion: There is a difference in antibacterial power between 6% cocoa peel extract and 5% NaOCl against Lactobacillus acidophilus.
Complex Aesthetic Treatment of Patients with Multiple Caries Devi Eka Juniarti; Tamara Yuanita; Nirawati Pribadi; Tarsardo Marbun; Nadien Sekar Pramesti
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.38-44

Abstract

Background: The need for dental esthetics is very important in recent years. Fulfilling aesthetic demands with both non-invasive and minimally invasive techniques can maintain tissue continuity. A 38 year old female patient came to the clinic with an esthetic complaint of maxillary anterior teeth. The following case report describes a step by step complex aesthetic treatment of a patient with multiple caries. Appealing aesthetic results will restore the patient's smile through restorative treatment in dentistry. Purpose: The purpose of this case report is to explain the management of complex aesthetic treatment of patients with multiple caries Case: A 38-year-old female patient complained her upper front teeth is turning black. The patient complained that her teeth had hurt ± 3 months ago and sometimes felt sore. The patient wants her teeth to be treated because she feels less confident when she smiles and wants to fix these teeth. The history of treatment on the tooth in question have had root canal treatment and been filled since 8 years ago. The tooth has had braces (orthodontic) treatment and was removed ± 7 years ago. The patient stated that she had no systemic health disorder. Case management : The treatment of this complex case were done using root canal treatment, endodontic retreatment, direct and indirect veneer, dowel, and crown. Conclusion: complex aesthetic treatment of patients with multiple were done aiming for good aesthetic result
Management of Root Perforation : A Case Report Sylvia Paulina Panggono; Teuku Chairil Hafiz; Tamara Yuanita
Conservative Dentistry Journal Vol. 12 No. 1 (2022): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i1.2022.53-56

Abstract

Background : Root perforation results in the communication between root canal walls and periodontal space (external tooth surface). It is commonly caused by an operative procedural accident or pathological alteration (such as extensive dental caries, and external or internal inflammatory root resorption). The diagnosis of dental pulp and/or periapical tissue previous to root perforation is an important predictor of prognosis. Case : A female patient came to Dental Conservative University of Airlangga with complaints of perforated lower left molars. On the objective examination obtained negative vitality test, positive on bite test. On radiographic examination, a radiolucent on bifurcation was performed Case management : The tooth was treated by hemisection. Root canal treatment was carried out in the distal canals. Afterwards, the tooth was split mesio-distally. The mesial root was extracted, and the distal root was retained and restored with PFM Crown. Discussion : Hemisection is a useful alternative treatment to extraction to save the multi-rooted teeth by endodontic approach, which includes the root canal treatment of the remaining roots and restoring them with suitable restorative material to splinting it with the adjacent tooth to decrease the risk of displacement followed by a fixed prosthodontic prosthesis to maintain the occlusal balance. Conclusion : Hemisection with subsequent prosthetic rehabilitation can be a viable alternative to retain severely decayed mandibular molar.
Rehabilitation of Anterior Teeth Trough Endorestoration Yuanita, Tamara; Chairil Hafiz, Teuku; Amalia Iqony, Rosa
Conservative Dentistry Journal Vol. 12 No. 2 (2022): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v12i2.2022.90-94

Abstract

Background: Nowadays, people has considered the importance of dental health, both in terms of functional and aesthetic improvements. Complex caries lesion of anterior teeth are a major problems that require aesthetic management due to patient's confidence. Purpose : The aim is to present a case of anterior maxillary teeth with complex caries treated with endodontic and restoration treatment to recover its optimal function aesthetically. Case A 18-year-old female patient with multiple caries on anterior maxillary teeth. She felt unconfidence about her performance. 13 class IV black caries lesion, 12 radicular tooth, 11 and 21 previously endodontic treated with metal post. 22 class V Black carieus lesion, 23 class III Black caries lesion. Case management:. 13, 12 and 22 were managed with root canal treatment and followed by fiber post insertion. 11 and 21 were previously endodontic treated and retreated with prefabricated fiber post. 23 with moderate caries was managed with crown. All anterior maxillary teeth had crown lengthening before. And all crown materials were made with Lithium Disilicate. Conslussion : Anterior maxillary teeth with severed multiple caries, successfully managed with optimal result. Restoring its function, form aesthetically and improve her quality of life.
Inhibitory effect of nano Stolephorus insularis and calcium hydroxide on glucosyltransferase (GTF) activity of Lactobacillus aciophilus Yuanita, Tamara; Juniarti, Devi Eka; Marbun, Tarsardo
Conservative Dentistry Journal Vol. 13 No. 1 (2023): January-June
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i1.2023.29-32

Abstract

Background: Dental caries is the most common multifactorial infectious disease worldwide which referes to the process of destruction tooth hard tissue caused by bacterial by-products in the form of acids through carbohydrate fermentation. Lactobacillus acidophilus is one of the main cariogenic bacteria that causes caries by forming biofilms with the help ofglucosyltransferase (GTF) enzymes. Calcium hydroxide has limited antibacterial effects. Nano brown anchovy contains fluor as the active compound which has the potential as an antibacterial alternative agent. Purpose: To explain the inhibition of the combination of nano Stolephorus insularis and calcium hydroxide on the activity of the glucosyltransferase enzyme of Lactobacillus acidophilus bacteria. Methods: This study used a combination of nano Stolephorus insularis 3.125% with calcium hydroxide as a treatment and aquades as a control. The GTF enzyme was obtained from the supernatant centrifuged by Lactobacillus acidophilus in BHIB. The activity of the GTF enzyme was considered by calculating the fructose levels using High Performance Liquid Chromatography (HPLC) in a certain formula.Results: Decreased levels of fructose was obtained in the treatment group. From the results of Mann- Whitney data analysis, there were significant difference in the study groups. Conclusion: the combination of nano Stolephorus insularis with calcium hydroxide is effective to inhibit the glucosyltransferase enzyme activity of Lactobacillus acidophilus bacteria.
Inhibition of Streptococcus mutans biofilm formation using combination of nano brown anchovy (Stolephorus insularis) and calcium hydroxide Yuanita, Tamara; Sukaton; Paramitha, Shafa Marwa Moza; Ulfadi, Bakhiitah Thufallah Putri; Salma, , Adinda Fazzahra; Setyabudi
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.69-73

Abstract

Background: One of the important virulence factors of S. mutans is the biofilm formation. Some bacteria in biofilms show resistance to antimicrobials. Therefore, the addition of nano brown anchovy (S. insularis) as antibiofilm agents can potentiate the caries and infection prevention efficacy of endodontic treatment. Purpose: To describe the effect of the combination of nano brown anchovy (S. insularis) 12% and Ca(OH)2 with a ratio of 1:1 on inhibition of S. mutans biofilm formation. Methods: Laboratory experimental research on S. mutans bacteria was carried out in vitro using the crystal violet assay method. Brown anchovy was made into nanoparticles, dissolved to a concentration of 12%, and combined with Ca(OH)2. Biofilm samples were given treatment and observed for inhibition of biofilm growth. Biofilm growth inhibition was seen from the Optical Density (OD)540nm absorbance value measured using a spectrophotometer. Results: In the group that was tested with Ca(OH)2, the average percentage in S. mutans biofilm reduction was 55%, while in the group that was tested with a combination of nano brown anchovy (S. insularis) and Ca(OH)2, the average percentage in S. mutans biofilm reduction was 61%. Conclusion: The combination of nano brown anchovy (S. insularis) 12% and Ca(OH)2 with a ratio of 1:1 can inhibit the formation of S. mutans biofilm.
Effect of combination of nano brown anchovy (Stolephorus insularis) nanoparticles and calcium hydroxide on inhibition Streptococcus sanguinis biofilm formation Yuanita, Tamara; Juniarti, Devi Eka; Nabilla, Tasya; Wiratno, Amelia Rahma; Tarigan, Shindyloken Juni Artha; Afifah, Anisa Nur; Setyabudi
Conservative Dentistry Journal Vol. 13 No. 2 (2023): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v13i2.2023.74-80

Abstract

Background: Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans are gram bacteria that can cause various problems in the oral cavity so that materials that have antibacterial potency are needed. Calcium hydroxide, chlorhexidine, and sodium hypochlorite as antibacterial ingredients have disadvantages such as forming tunnel defects, causing dysgeusia, and damaging periapical tissue, so it is hoped that there will be natural materials as alternatives. The nano brown anchovy has fluor as the active compound which has the potential as an antibacterial agent. Purpose: To analyze the effect of antibacterial potency of nano brown anchovy (Stolephorus insularis) on Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans. Methods: This research is a laboratory experimental in vitro with the post-test only control group design. Brown anchovy is made into nano and diluted by dilution method into several concentrations. The direct contact method was used between some concentrations and the two bacteria. The values of MIC (Minimum Inhibitory Concentration) and MBC (Minimum Kill Concentration) were obtained by counting the number of bacterial colonies on Mueller Hinton Agar media. Bacterial colony growth was calculated manually in Colony Forming Units (CFU). Results: Antibacterial potency of nano brown anchovy on Lactobacillus acidophilus showed MIC results at 1.56% concentration and MBC at 3.125% concentration. Antibacterial potency of nano brown anchovy on Aggregatibacter actinomycetemcomitans showed MIC results at 3.125% concentration and MBC at 6.25% concentration. Conclusion: Nano brown anchovy has antibacterial activity on Lactobacillus acidophilus and Aggregatibacter actinomycetemcomitans.
Co-Authors Adioro Soetojo Aditya Rama Devara Afifah, Anisa Nur Agus Subiwahjudi Agus Subiwahjudi Agus Subiyanto Alissa Amanda Amalia Iqony, Rosa Anandita, Tiara Ari Subiyanto Ari Subiyanto, Ari Arindah Hadi Bin Sharizal, Shafy Shariz Cecilia G.J Lunardhi Cecilia lunardhi Chairil Hafiz, Teuku Charles, William Christabel, Phebe Fedora Deavita Dinari Devi Eka Juniarti Dian Agustin Wahjuningrum Dina Ristyawati Edhie Arief Prasetyo Edhie Arif Pasetyo Edhie Arif Prasetyo Eric Priyo Prasetyo Fajar Agus Muttaqin Fajariana Fitriani Firmansyah, Awang Bagus Galih Sampoerno Hadriany Hotmaria Ika Tangdan Jesica Ceren Karlina Samadi Ketut Suardita, Ketut Kuntaman Kuntaman Latief Mooduto, Latief Liong, Michelle M. Mudjiono M. Roelianto Mandojo Rukmo Marbun, Tarsardo Marpaung, Grace Julieta Mega Selvia Mifta Izha A R Mohammad Roelianto Mohammed Alaqsha Brysoul Ceson Nabilla, Tasya Nadien Sekar Pramesti Nanik Zubaidah Nila Murni Wulandari Nirawati Pribadi Paidal, Nurfahira Paramitha, Shafa Marwa Moza Prasetyaningtias, Silvia Arlita Hayu Prasita, Shafa Qintan Sekar Adjani R. Roulianto Randy Carlos Sietho Rega Maurischa Arantika Putri Reinold Christian Lina Revina Ester Iriani Marpaung Richard Fritzgerald Rifatul Jannah Ruslan Effendy Ruslan Effendy Salma, , Adinda Fazzahra Sepdhyo Wahyu Nugroho Setyabudi Setyabudi Setyabudi Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Sylvia Paulina Panggono Tania, Cindy Grace Tantri Wismayaning Radito Tarigan, Shindyloken Juni Artha Tarsardo Marbun Teuku Chairil Hafiz Ulfadi , Bakhiitah Thufailah Putri Ulfadi, Bakhiitah Thufallah Putri Uli Sasi Andari Wiratno, Amelia Rahma Yuliana Dwiwahyu Suryandari Yulianti Kartini Sunur