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All Journal Dental Journal (Majalah Kedokteran Gigi) Conservative Dentistry Journal Indonesian Journal of Dental Medicine

Tamara Yuanita

Conservative Dentistry Department, Faculty Of Dentistry, Universitas Airlangga,

Author-ID : 3197033

Dentistry

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EKSPRESI TGF-B DAN MMP-1 PADA PERIODONTITIS APIKALIS KRONIS AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS TIKUS WISTAR Tamara Yuanita; Hadriany Hotmaria; Ruslan Effendy; Ketut Suardita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of TGF-ß to enhance the proliferation collagen and MMP-1 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of of TGF-ß and MMP-1 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 27 male rats were randomly divided into 3 main groups. Group A (negative control) : every tooth was’nt induced by anything. Group B ( positive control): every tooth was induced only by sterile BHIb and closed by GIC Fuji II as the final restoration. Group C (: every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), and closed by GIC Fuji II as the final restoration. The animals were sacrificed after 21 days and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-1 increased significantly in group C when E.faecalis was induced. When expression of TGF-ß decreaced significantly in group C rather than group B. Conclusion. From this study we know that the expression of TGF-ß and MMP-1 are make opposite pathway due to chronic apical periodontitis that induced by E.faecalis.

EKSPRESI MATRIKS METALLOPROTEINASE-8 DAN INTERLEUKIN-8 PADA KERUSAKAN JARINGAN PERIAPIKAL AKIBAT INDUKSI BAKTERI ENTEROCOCCUS FAECALIS Tamara Yuanita; Tantri Wismayaning Radito; Dian Agustin wahjuningrum; R. Roulianto
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation and destruction. It occurs due to the capability of IL-8 to enhance the inflamation reaction and MMP-8 to stop the collagen formation. The ability of enterococcus faecalis in enhancing inflamation process cause host can not reach the homeostasis phase and performing an even bigger tissue damage. Purpose. The aim of this study is to know about the expression of MMP-8 and IL-8 during the periapical tissue damage due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of tissue damage, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of MMP-8 and IL-8 increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of MMP-8 and IL-8 are increasing during the periapical tissue damage that induced by E.faecalis.

EKSPRESI Nuclear Factor of Activated T cells c-1 (NFATc-1) DAN OSTEOKALSIN PADA KERUSAKAN TULANG PERIAPIKAL AKIBAT INDUKSI BAKTERI Enterococcus faecalis Arindah Hadi; M. Roelianto; Ari Subiyanto; Tamara Yuanita
Conservative Dentistry Journal Vol. 7 No. 2 (2017): July - December
Publisher : Universitas Airlangga

Background. The main etiology of endodontic treatment failure is caused by bacteries that stay in the root canal. E.faecalis is a bactery that is found as an etiology of endodontic treatment failure. Cell wall of this bacteria is containing Lipoteichoic acid (LTA). LTA can penetrate into the periradicular tissue, act as endotoxin in host and cause periradicular inflammation then lead to bone destruction. Bone destruction occurs due to the inflammation process that is mediated by immune system. The important cell in the process of bone destruction is osteoclast. Bone destruction is marked by the form of osteoclast that is called osteoclastogenesis. NFATc-1 and osteocalcin play important things in osteoclastogenesis. Purpose. The aim of this study is to know about the expression of NFATc-1 and osteocalcin during the periapical bone destruction due to induction of E.faecalis. Method. This study used laboratory experimental with the post test only control group design. A total of 54 male rats were randomly divided into 2 main groups, which each main group had 3 subgroups. Group A (control) : every tooth was induced only by sterile BHIb. Group A had 3 subgroups (A Control day 3, 10, and 21), group B : every tooth was induced by 10 μl BHI-b E.faecalis ATCC212(106 CFU), it was contained 3 sub groups (B day 3,10, and 21). The animals were sacrificed based on their days scheduled group and prepared for histological examination of periapical bone, then we did the immunohistochemistry followed by calculation on the light microscope. Result. The analysis revealed that the expression of NFATc-1 and osteoclast increased significantly in group B when E.faecalis was induced. Conclusion. From this study we know that the expression of NFATc-1 and osteocalcin are increasing during the periapical bone destruction that induced by E.faecalis.

Perbedaan Daya AntibakteriEkstrak Kulit Kokoa (Theobroma cacao)dan NaOCl 2,5% terhadapPorphyromonas gingivalis Tamara Yuanita; Rifatul Jannah; Edhie Arif Pasetyo; Setyabudi Setyabudi
Conservative Dentistry Journal Vol. 8 No. 1 (2018): January - June
Publisher : Universitas Airlangga

Background: Since pulp infection plays an important role in the development of periradicular lesions, endodontic treatment should be directed to eliminate bacterial and theirproducts. However, currently 20% of cases of apical periodontitis are not resolved after root canal treatment and therefore required for new root canal disinfection. The most commonly used irrigation material today is NaOCl 2.5%. However, NaOCl has negative effects, including being toxic when the material is injected into the periradicular tissue causing extensive pain, bleeding and swelling. Until now, many drugs come from plants that are still produced from plant extracts. One of the plants that can be utilized is cocoa (Theobroma cacao). Cocoa contains active compounds, such as saponins, tannins, alkaloids, flanonoids, aromatic terpenoids, theobromins and other metabolites. Cocoa husk has been studied to have an antibacterial effect on Porphyromonas gingivalis which is the main bacterial cause of apical periodontal. However, the difference in antibacterial activity between cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis has not been studied. Porpuse:The aim of this study is to compare antibacterial activity of cocoa husk extract and NaOCl 2.5% againstPorphyromonas gingivalis.Method: This research was a laboratory experimental study. Porphyromonas gingivalis were swabbed to nutrient agar medium. Consequently, cocoa husk extract 25% and NaOCl 2.5% were placed in wells of 5mm diameter and nutrient agar medium. The diameter of the zone of inhibition around the test materials was measured after 24 hours.Result:Cocoa husk extract has lower mean inhibitory zone diameter (14.22) than NaOCl 2.5% (16.06). Conclusion:Cocoa husk extract has lower antibacterial activity against Porphyromonas gingivalis compared to NaOCl 2.5%.

Antibakteri Ekstrak Kulit Buah Kakao (Theobroma cacao) 6,25% dan NaOCl 2,5% Terhadap Bakteri Streptococcus sanguinis Sepdhyo Wahyu Nugroho; Mandojo Rukmo; Edhie Arief Prasetyo; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococcus sanguinis is a gram-positive bacterium that infects and penetrates into dentinal tubules from a depth of 150 μm up to 792 μm. Chemicals, namely 2.5% NaOCl, are used to mitigate the growth of these bacteria via irrigation of the root canals, but 2.5% NaOCl still has disadvantages including irritating periradicular tissue, having an unpleasant odor, and being toxic. Due to these shortcomings, natural materials are expected to be used as alternatives. Cocoa peel extract has active tannin compounds, flavonoids, alkaloids, terpenoids, and saponins which have antibacterial attributes; a concentration of 6.25% is counted as the minimum bactericidal concentration (MBC) of Streptococcus sanguinis. Aim: To compare the difference of antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) and 2.5% NaOCl against Streptococcus sanguinis. Method: This research is an in vitro experimental laboratory with post-test only control group design. The diffusion method was used on Streptococcus sanguinis planted in tubes containing BHIB, then cultured on petri dishes containing nutrient agar and divided into 3 parts, namely 6.25% cocoa peel extract, 2.5% NaOCl, and negative controls, then each Petri dishes were given a paper disc that had been dripped by 0.01 ml of each ingredient, then incubated in an incubator for 2x24 hours at 37°C; the diameter of the inhibitory zone formed was then observed using a caliper. Results: The average diameter of the inhibition zone formed on 6.25% cocoa peel extract was 19.2000 mm and 2.5% NaOCl was 17.2813 mm against Streptococcus sanguinis. Conclusion: The antibacterial power of 6.25% cocoa peel extract (Theobroma cacao) is higher than 2.5% NaOCl against Streptococcus sanguinis.

The Difference Of Antibacterial Power Between Cocoa Peel (Theobroma cacao L.) Extract 6,25% and Chlorhexidine 0,2% Againts Streptococcus sanguinis Nila Murni Wulandari; Edhie Arif Prasetyo; Agus Subiwahjudi; Tamara Yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Background: Streptococcus sanguinis is a bacterium that can cause failures in root canal treatments due its ability to penetrate the dentinal tubules to a depth of 400 μm in just two weeks. Irrigation material is needed to stop the growth of this bacteria so that no bacteria can pass through by using chemicals, irrigation materials that are widely used such as Chlorhexidine 0,2% but still lack because it cannot be used as a single irrigation solution because its effectiveness will be reduced if it is related to protein and organic dentine matrix and low Ph saliva.Therefore, research is needed to find natural ingredient that can be an alternative such as Cocoa peel extract was chosen because it contains active compounds, in the form of saponins, tanins, alkaloids, flavonoids, and terpenoids that have been known to have antibacterial properties a concentration of 6,25% is used in accordance with the MKC of Streptococcus sanguinis. Aim: To compare the antibacterial power between cocoa peel extract (Theobroma cacao L.) 6,25% and Chlorhexidine 0,2% against Streptococcus sanguinis Method: This research is an experimental laboratory with a post-test only control group design. The diffusion method was used to determine the susceptibility of bacteria isolated from the material by planting the culture of Streptococcus sanguinis on the agar medium by swabbing the nutrient media which has been divided into 3 parts consisting of negative control, cocoa peel extract and Chlorhexidine, then each nurient media so that it is given a paper disk and 0.01ml liquid on each section. The diameter of the inhibition zone was observed after 2x24 hours using the calipers. Results: The average inhibitory zone that was formed using cocoa peel extract was 20,40 mm against Streptococcus sanguinis and Chlorhexidine was 18,36 against Streptococcus sanguinis. Conclusion: Cocoa peel extract (Theobroma cacao L.) 6,25% had higher anti-bacterial power compared to 0,2% Chlorhexidine against the growth of Streptococcus sanguinis.

Sitotoksisitas Ekstrak Kulit Kakao (Theobroma cacao) terhadap Kultur Sel Fibroblas BHK-21 Fajariana Fitriani; Adioro Soetojo; Agus Subiwahjudi; tamara yuanita
Conservative Dentistry Journal Vol. 9 No. 1 (2019): January - June
Publisher : Universitas Airlangga

Latar Belakang: Irigasi saluran akar merupakan salah satu tahapan penting untuk menunjang keberhasilan perawatan. Sodium hipoklorit (NaOCl) merupakan larutan irigasi utama yang sering digunakan namun memiliki sejumlah kekurangan yakni bersifat toksik jika diirigasi sampai ke jaringan periradikular. Kakao (Theobroma cacao) merupakan tumbuhan suku Sterculiaeae yang kulit buahnya mengandung senyawa alkaloid, flavonoid, tanin, saponin dan terpenoid. Senyawa-senyawa tersebut terbukti dapat menghambat pembentukan biofilm pada bakteri Enterococcus faecalis yang merupakan bakteri paling resisten pada saluran akar. Ekstrak kulit kakao diharapkan dapat menjadi bahan alternatif irigasi saluran akar yang ideal, sehingga perlu dilakukan penelitian mengenai efek sitotoksisitasnya terhadap jaringan. Tujuan: Menentukan konsentrasi dari ekstrak kulit kakao yang memberikan efek sitotoksik pada sel fibroblas BHK-21. Metode: Jenis penelitian yang dilakukan adalah eksperimental laboratoris dengan menggunakan kultur sel fibroblas BHK-21. Ekstrak kulit kakao diperoleh melalui maserasi menggunakan etanol 70% dan dilakukan pengenceran dengan konsentrasi 1,56%, 3,125%, 6,125%, 12,5%, 25%, 50%, dan 100%. Sel BHK-21 dalam microplate 96 well dipaparkan dengan ekstrak kulit kakao. Uji sitotoksisitas menggunakan MTT assay dan absorbansi warna dibaca menggunakan Elisa reader. Nilai absorbansi dihitung dengan rumus sehingga didapatkan hasil akhir berupa persentase kematian sel. Hasil: Peningkatan konsentrasi ekstrak kulit kakao berbanding lurus dengan kenaikan persentase sel fibroblas BHK-21 yang mati. Kesimpulan: Konsentrasi minimum ekstrak kulit kakao yang dapat memberikan efek sitotoksik pada sel fibroblas BHK-21 adalah 6,25%.

The effect of theobromine and NaF 2% exposure to enamel surface hardness after immersing in orange juice beverage Tamara Yuanita; Setyabudi Setyabudi; Qintan Sekar Adjani
Conservative Dentistry Journal Vol. 9 No. 2 (2019): July - December
Publisher : Universitas Airlangga

Background: Consumption of orange juice which had acidic quality will cause a demineralization on enamel. The most effective way to prevent the demineralization process was by involving remineralization agents. Fluoride was effective to improve remineralization but has toxicity effect and caused fluorosis at certain dose. Another alternative for remineralization agent without providing side effects is theobromine contained in cocoa peel extract (Theobroma cacao). Objective: To explain the effect of theobromine and NaF 2% exposure on enamel surface hardness after immersion in commercial orange juice. Method: Twenty-seven cattle incisors were cut into a square shape 1x1 cm and then planted in a round resin mold then divided into three groups. All three groups were immersed in orange juice beverage for 60 minutes. The control group was immersed in distilled water, group I was given theobromine 200 mg / L, and group II was given 2% NaF after exposure for 96 minutes. Surface hardness measurements were carried out using the Wolpert Micro Vickers Testers tool. Results: There was a significant difference (p <0.05) between surface hardness of tooth enamel from the control group and theobromine group and there was also a significant difference between the control group and the NaF group. However, there was no significant difference (p> 0.05) in the NaF group with theobromine group. Conclusion: The effect of exposure to theobromine and NaF 2% on surface enamel hardness after immersion in orange juice beverage has the same result.

Enamel Hardness Differences After Topical Application Of Theobromine Gel And Casein Phosphopeptide-Amorphous Calcium Phosphate Tamara Yuanita; Nanik Zubaidah; Mifta Izha A R
Conservative Dentistry Journal Vol. 10 No. 1 (2020): January-June
Publisher : Universitas Airlangga

Background: Most soft drinks contain citric acid / phosphoric acid with pH <3.5. Strong acids in the oral cavity cause tooth erosion. Tooth erosion leads to demineralization enamel. Remineralization can prevent demineralization process. One of the commercial remineralization agents is Casein Phosphopeptide-amorphous Calcium Phosphate(CPP-ACP) but it has low solubility and less affordable price. Therefore, a natural ingredients such as theobromine from cocoa extract need to be developed as remineralization agent. Objective: To determine enamel hardness differences due to topical application of theobromine gel and CPP-ACP. Method: incisive crowns were cut off (1x1) cm then planted in cylindrical acrylic resin. All samples were immersed with citric acid for 90 minutes then divided into 3 groups. Group I (control) was immersed with aquades (96 min), group II was applied with theobromin gel 200 mg/l (96 min), group III was applied with CPP-ACP (96 min). By using vickers hardness tester, enamel hardness be measured (15 sec) at 3 points. Results: There were significant differences between the control group, CPP-ACP group, and theobromine gel group. Conclusion: Enamel hardnesse with theobromine gel application is higher than CPP-ACP application..

DIFFERENCES IN SURFACE HARDNESS OF BOVINE ENAMEL AFTER EXPOSURE OF THEOBROMINE, ALKALOID COCOA POD EXTRACT AND ACIDULATED PHOSPHATE FLUORIDE Tamara Yuanita; Nanik Zubaidah; Jesica Ceren
Conservative Dentistry Journal Vol. 10 No. 2 (2020): July-December
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/cdj.v10i2.2020.71-74

Background: Acid diet could process demineralization on enamel surface. Process remineralization needed to prevent caries occurrence. The excessive fluorine usage could induce adverse side effects, therefore the development of natural resources such as theobromine and alkaloid cocoa extract as a remineralization is needed. Purpose: To discover the differences of bovine teeth enamel surface hardness after exposure to theobromine, alkaloid cocoa pod extract and acidulated phosphate fluoride. Method: Twenty-eight tooth crowns of bovine insisivus cut into the shape of the square then planted on the resin mold round shape and divided into four groups. The control group without treatment, the group I was exposed APF, the group II was exposed 1000 mg/L alkaloid cocoa pod extract, group III was exposed 200 mg/L Theobromine 200 mg/L. Surface hardness measured using Wolpert Wilson Vickers Microhardness Tool. Results: The group of theobromine produces the highest enamel surface hardness. The enamel surface hardness in alkaloid group of cocoa pod extract was higher compared to the APF group and the control group. Conclusion: The hardness surface of bovine tooth enamel after exposure to theobromine and alkaloid cocoa pod extract is harder than exposure to acidulated phosphate fluoride.

Co-Authors Adioro Soetojo Aditya Rama Devara Afifah, Anisa Nur Agus Subiwahjudi Agus Subiwahjudi Agus Subiyanto Alissa Amanda Amalia Iqony, Rosa Anandita, Tiara Ari Subiyanto Ari Subiyanto, Ari Arindah Hadi Bin Sharizal, Shafy Shariz Cecilia G.J Lunardhi Cecilia lunardhi Chairil Hafiz, Teuku Charles, William Christabel, Phebe Fedora Deavita Dinari Devi Eka Juniarti Dian Agustin Wahjuningrum Dina Ristyawati Edhie Arief Prasetyo Edhie Arif Pasetyo Edhie Arif Prasetyo Eric Priyo Prasetyo Fajar Agus Muttaqin Fajariana Fitriani Firmansyah, Awang Bagus Galih Sampoerno Hadriany Hotmaria Ika Tangdan Jesica Ceren Karlina Samadi Ketut Suardita, Ketut Kuntaman Kuntaman Latief Mooduto, Latief Liong, Michelle M. Mudjiono M. Roelianto Mandojo Rukmo Marbun, Tarsardo Marpaung, Grace Julieta Mega Selvia Mifta Izha A R Mohammad Roelianto Mohammed Alaqsha Brysoul Ceson Nabilla, Tasya Nadien Sekar Pramesti Nanik Zubaidah Nila Murni Wulandari Nirawati Pribadi Paidal, Nurfahira Paramitha, Shafa Marwa Moza Prasetyaningtias, Silvia Arlita Hayu Prasita, Shafa Qintan Sekar Adjani R. Roulianto Randy Carlos Sietho Rega Maurischa Arantika Putri Reinold Christian Lina Revina Ester Iriani Marpaung Richard Fritzgerald Rifatul Jannah Ruslan Effendy Ruslan Effendy Salma, , Adinda Fazzahra Sepdhyo Wahyu Nugroho Setyabudi Setyabudi Setyabudi Sholeh Ardjanggi Sukandar, Wilson Sukaton, Sukaton Sylvia Paulina Panggono Tania, Cindy Grace Tantri Wismayaning Radito Tarigan, Shindyloken Juni Artha Tarsardo Marbun Teuku Chairil Hafiz Ulfadi , Bakhiitah Thufailah Putri Ulfadi, Bakhiitah Thufallah Putri Uli Sasi Andari Wiratno, Amelia Rahma Yuliana Dwiwahyu Suryandari Yulianti Kartini Sunur

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