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All Journal Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) AGRIVITA, Journal of Agricultural Science BERITA BIOLOGI JURNAL BIOLOGI INDONESIA Jurnal Biodjati ANNALES BOGORIENSES Biosaintifika: Journal of Biology & Biology Education Journal of Tropical Crop Science AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Biofarm : Jurnal Ilmiah Pertanian Annales Bogorienses
Enung Sri Mulyaningsih
Research Center for Biotechnology, Lembaga Ilmu Pengetahuan Indonesia, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Economics, Econometrics & Finance Environmental Science Immunology & microbiology Medicine & Pharmacology

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Journal : jurnal biologi indonesia

 1 
Keragaman Morfologi dan Genetik Padi Gogo Lokal Asal Banten Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (398.926 KB) | DOI: 10.14203/jbi.v10i1.337

Abstract

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype
Eksplorasi Marka SSR Terpaut Sifat Toleransi Padi Gogo terhadap Alumunium Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3099

Abstract

ABSTRACTThe objective of this research was to study molecular markers were linked to upland rice tolerance of Situ Patenggang x B11930F-TB2 against Al using SSR method. The plant materials were 36 lines of Situ Patenggang x B11930F-TB2 with categories tolerant, moderate, and susceptible to acid soil based on selection in East Lampung. IR6008032 line and ITA variety used as comparative control of tolerant and susceptible. The results showed that 40 primer pairs were applied to 36 lines and allegedly 3 primer pairs that are: RM205, RM257 and RM247 linked to Al tolerance. The markers location analysis on rice chromosome listed as follows: RM205 and RM257 were located on chromosome 9 and RM247 located on chromosome 12 with genetic distance 93,12 cM, 772.62 cM and 13,05 cM, respectively.Keywords: SSR marker, upland rice, aluminium tolerance, ultisols
KERAGAMAN MORFOLOGI DAN GENETIK PADI GOGO LOKAL ASAL BANTEN Mulyaningsih, Enung Sri; Indrayani, Sri
JURNAL BIOLOGI INDONESIA Vol 10, No 1 (2014): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v10i1.337

Abstract

Superior upland rice cultivar can be assembled through crossbreeding using germplasm diversity which serves as a sourceof gene. Germplasm may constitute of landrace and local cultivars. Banten is one of the provinces that has various localupland rice varieties. Baduy tribe, in the province has played a major role in preserving local upland rice varieties anduphold the wisdom.Genetic diversity information and the benefits of local cultivars can be useful to maximize the selectionprocess of parental candidate for creating new cultivars. This study aims to determine the genetic diversity of local uplandrice from Banten. Methods of analysis diversity performed by phenotypic and genotypic. Observations phenotypic throughobservation of agronomic characters and yield of each cultivar. Whereas genotypic observations conducted with RAPD(random amplified polymorphism DNA). In this study we used 17 local upland rice cultivars from Banten with 14 randomprimers for RAPD. The result showed a mean plant height local upland rice cultivar of Banten was higher than nationalupland rice varieties however productive tillers and rice productivity were lower than national varieties. Nonetheless, localcultivars persisted in this environment because it has the advantage that the community needed. Based on the time ofharvest, local cultivar belonged to early and medium maturing group. Qualitative trait observation showed that there werediversity of stem, leaves, grain and rice among the cultivars. Genetic analysis with RAPD showed nine primers out of 14were successful in amplifying loci from DNA template. The primers having high amplification product were OPJ 05, OPJ07 and OPD07 but the highest polymorphism presentation was OPJ 01. Based on phylogenetic tree, there were two mainclusters. Padi Koneng and Carogol were similar. Three glutinous rice cultivars (Mayang, Jalupang, and Hideung) were inone cluster except Kentan Simpai.Keywords: upland rice, Banten, RAPD, Phenotipe, genotype
EKSPLORASI MARKA SSR TERPAUT SIFAT TOLERANSI PADI GOGO TERHADAP ALUMUNIUM Anggraheni, Yuliana Galih Dyan; Mulyaningsih, Enung Sri
JURNAL BIOLOGI INDONESIA Vol 13, No 1 (2017): JURNAL BIOLOGI INDONESIA
Publisher : Perhimpunan Biologi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/jbi.v13i1.3099

Abstract

ABSTRACTThe objective of this research was to study molecular markers were linked to upland rice tolerance of Situ Patenggang x B11930F-TB2 against Al using SSR method. The plant materials were 36 lines of Situ Patenggang x B11930F-TB2 with categories tolerant, moderate, and susceptible to acid soil based on selection in East Lampung. IR6008032 line and ITA variety used as comparative control of tolerant and susceptible. The results showed that 40 primer pairs were applied to 36 lines and allegedly 3 primer pairs that are: RM205, RM257 and RM247 linked to Al tolerance. The markers location analysis on rice chromosome listed as follows: RM205 and RM257 were located on chromosome 9 and RM247 located on chromosome 12 with genetic distance 93,12 cM, 772.62 cM and 13,05 cM, respectively.Keywords: SSR marker, upland rice, aluminium tolerance, ultisols
Co-Authors Adi, Eko Binnaryo Mei Agus Arfani, Agus Ambar Yuswi Perdani, Ambar Yuswi Anggraheni, Yuliana Galih Dyan Anggraheni, Yuliana Galih Dyan Anjarsari, Nurul Aeni Anky Zannati Apriadi Situmorang Burhana, Nana Dedi Supriadi Didy Sopandie Dody Priadi Dwi Astuti Dwi Widyajayantie Febriana Dwi Wahyuni Fiqolbi Nuro Pohan, Fiqolbi Nuro Fiqolbi Nuro, Fiqolbi HAJRIAL ASWIDINNOOR Hani Fitriani, Hani Heru Wibowo Inez Hortense Slamet Loedin IWAN SASKIAWAN Kurniawan Rudi Trijatmiko, Kurniawan Rudi N. Sri Hartati, N. Sri Ningsih, Irnawati OuwerkerV, Pieter BF Pantouw, Carla F. Pantouw, Carla Frieda Paradisa, Yashanti Berlinda Pieter B.F. Ouwerkerk Prita Sari Dewi Puspita Deswina, Puspita Rachmawati, Syamsidah Rahman, Nurhamidar Satya Nugroho Sintho Wahyuning Ardie Slamet Loedin, Inez Hortense Sri Indrayani, Sri Sri Rianawati Sudarsono Syamsidah Rahmawati, Syamsidah TRI JOKO SANTOSO Ulfa, Umi Tahniah Vincentia Esti Windiastri, Vincentia Esti Widyajayanti, Dwi Yuli Sulistyowati