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All Journal Jurnal Akuakultur Indonesia Jurnal Mina Sains Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Jurnal Perikanan
Muhammad Arif Mulya
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Economics, Econometrics & Finance Social Sciences Veterinary

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PEMBENIHAN IKAN KOI CYPRINUS RUBROFUSCUS Cyprinus rubrofuscus (Lacepede, 1803) DI MINA KARYA KOI, KABUPATEN SLEMAN, DAERAH ISTIMEWA YOGYAKARTA: Hatchery and Intermediate Rearing of Koi Carp Cyprinus Rubrofuscus (Lacepede, 1803) at Mina Karya Koi, Sleman Regency, Special Region of Yogyakarta Muhammad Arif Mulya; Giri Maruto Darmawangsa; Ratu Mutiara Wali; Santoso Santoso
Jurnal Sains Terapan : Wahana Informasi dan Alih Teknologi Pertanian Vol. 11 No. 2 (2021): Jurnal Sains Terapan, Volume 11 Nomor 2, Tahun 2021
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (291.912 KB) | DOI: 10.29244/jstsv.11.2.86-101

Abstract

ABSTRACTThe introduced freshwater ornamental fish commodity which is a favorite in the international market with relatively stable prices in the market is the koi fish Cyprinus rubrofuscus. The average increase in koi fish production is 11,6% every year. The koi fish observed were showa. The broodstock of koi fish are fed in the form of Shoori Superior Koi Food Spirulina variant with the ad satiation method. The average eggs produced in spawning were 288,300 eggs with FR 78,3%, and HR 87,98%. Koi fish hatchery activities produce 2-3 cm larvae in 14 days and produce 5-10 cm in 45 days. Mina Karya Koi is able to produce quality larvae and seeds as many as 147,000 tail of fish for a year with an SR (Survival Rate) produced for 45 days of 85,6%. This hatchery activity has an R/C ratio of 2,5 with a selling price of larvae of Rp150 000 per kg and a selling price of seeds of Rp3 000 per tail of fish.Key words : Koi Fish, Cyprinus rubrofuscus, Hatchery ABSTRAKKomoditas ikan hias air tawar introduksi yang menjadi favorit di pasar internasional dengan harga relatif stabil di pasar adalah ikan koi Cyprinus rubrofuscus. Kenaikan rata-rata produksi ikan koi sebesar 11,6% per tahun. Ikan koi yang diamati adalah ikan koi jenis showa. Induk ikan koi diberikan pakan berupa Shoori Superior Koi Food varian Spirulina dengan metode ad satiation atau sekenyangnya. Rata-rata telur yang dihasilkan dalam pemijahan yaitu sekitar 288.300 butir telur dengan FR 78,3%, dan HR 87,98%. Kegiatan pembenihan ikan koi menghasilkan larva ukuran 2-3 cm dalam waktu 14 hari dan menghasilkan benih ukuran 5-10 cm dalam waktu 45 hari. Mina Karya Koi mampu menghasilkan larva dan benih yang berkualitas sebanyak 147.000 ekor per tahun dengan SR (Survival Rate) yang dihasilkan selama 45 hari sebesar 85,6%. Kegiatan pembenihan ini memiliki R/C ratio sebesar 2,5 dengan harga jual larva sebesar Rp150 000 per kg dan harga jual benih sebesar Rp3 000 per ekor.Kata kunci : Ikan Koi, Cyprinus rubrofuscus, Pembenihan
APLIKASI HIDROGEN PEROKSIDA DI SEDIMEN TERHADAP KUALITAS AIR DAN PERTUMBUHAN UDANG VANAME Litopenaeus vannamei Wiyoto Wiyoto; Satrio Yudha Wisesa; Wida Lesmanawati; Muarif Muarif; Andri Iskandar; Andri Hendriana; Cecilia Eny Indriastuti; Muhammad Arif Mulya; Dian Eka Ramadhani; Ima Kusumanti; Amalia Putri Firdausi; Dudi Lesmana
JURNAL MINA SAINS Vol. 9 No. 1 (2023): Jurnal Mina Sains
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/jmss.v9i1.8138

Abstract

Pond bottom sediment conditions influence shrimp culture. As benthic organisms, shrimp are mainly on the surface of the pond bottom, so cultivation preparations related to improving sediment quality are essential. Pond bottom practice, such as drying, liming, and fertilizing, is common. However, in bottom pond conditions where total drying cannot be carried out, other treatments are needed, such as the addition of hydrogen peroxide. The purpose of this study was to determine the effect of adding hydrogen peroxide in pond sediments on the performance of shrimp growth and water quality. The study was conducted with five doses of hydrogen peroxide (0.25 mL, 0.5 mL, 1 mL, 2 mL) mixed with 350 g of pond soil sediment. Ten shrimps with an average weight of 0,27±0,03 g were put into the rearing container, which had been given pond bottom sediment according to the treatment. The results showed that the 2 mL hydrogen peroxide treatment gave the best results on the growth rate and average weight of Pacific whiteleg shrimp at the end of rearing. Water quality such as pH value, dissolved oxygen, and temperature were not significantly different between treatments. Hydrogen peroxide treatment can improve shrimp growth performance without affecting water quality during rearing.
Teknologi Budidaya Ikan Badut Amphiprion Sp. Di Balai Besar Perikanan Budidaya Laut (Bbpbl), Lampung Andri Iskandar; Muhamad Erlan Hafid Djunaedi; Muhammad Arif Mulya; Dian Eka Ramadhani; Nazla Wafi; Yuli Yulianti
Jurnal Perikanan Unram Vol 14 No 3 (2024): JURNAL PERIKANAN
Publisher : Universitas Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jp.v14i3.869

Abstract

Clownfish Amphiprion sp., have a relatively wide distribution area, especially in Indo-Pacific waters. In nature these fish live in small groups in one anemone and consist of one pair of parents, the others are usually smaller in size which are still in the male sex. If one of the parent pairs dies, the position will be replaced by the largest in the group, especially the female. This condition is usually called sequential hermaphrodite. The purpose of this study was to gather information on the techniques for fish hatchery clownfish to produce quality fish. Clownfish spawn with a sex ratio of 1:1, with an average spawning frequency of 2 times a month. Clown fish can produce eggs with an average of 419 eggs, fertilization rate (FR) 76,37%, hatching rate (HR) 75,27%, and survival rate (SR) 58,35%. For one year, the total number of fish hatchery that can be produced is 108.911. Payback period of 8,2 years, break event point (unit) of 73.859 fish/year, break event point (rupiah) of Rp. 369.292.918, and cost of production of Rp. 3.817/fish
Characterization and molecular detection of pathogenicity and antibiotic resistance genes in Vibrio parahaemolyticus isolated from Pacific white shrimp Muhammad Arif Mulya; Fachriyan Hasmi Pasaribu; Usamah Afiff; Munti Yuhana
Jurnal Akuakultur Indonesia Vol. 21 No. 1 (2022): Jurnal Akuakultur Indonesia
Publisher : Indonesian Society of Scientific Aquaculture (ISSA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19027/jai.21.1.81-92

Abstract

ABSTRACT This study aimed to conduct the characterization and molecular detection of the pathogenicity and antibiotic-resistance genes in Vibrio parahaemolyticus, as the causative agent of vibriosis in Pacific white shrimp. The V. parahaemolyticus isolates were collected from the shrimp’s hepatopancreas, before biochemical test and polymerase chain reaction (PCR) sequencing of the 16S rRNA gene confirmation. The hemolysis test and PCR were applied to detect the presence of virulence genes, namely toxR, thermostable direct haemolysin (tdh), and tdh-related haemolysin (trh). The Kirby-Bauer method was used for characterizing the resistance patterns against ampicillin (AMP), tetracycline (TET), cyprofloxacin (CIP), enrofloxacin (ENR), and chloramphenicol (CHL). The biochemical tests and PCR-16SrRNA gene sequencing confirmed that 12 isolates belonged to V. parahaemolyticus that were further verified by amplification of the toxR gene in 382 bp (100% of the isolates). The alpha hemolysis activity was also confirmed by the amplicon of 199 bp in all isolates. All V. parahaemolyticus isolates showed their resistance to AMP and 42% of the isolates were TET-resistant. However, no resistance was shown to CIP, ENR, and CHL. The PCR-based analysis resulted a detectable resistance gene of ampC (42% of the isolates) and tetB (83% of the isolates). Keywords: antibiotics, shrimp, resistance, virulency, Vibrio parahaemolyticus ABSTRAK Penelitian ini bertujuan untuk melakukan karakterisasi dan deteksi molekular dari gen patogenisitas dan resistansi antibiotik pada Vibrio parahaemolyticus, agen penyebab vibriosis pada udang vaname. Isolat V. parahaemolyticus dikoleksi dari hepatopankreas, diuji secara biokimiawi dan selanjutnya dikonfirmasi dengan polymerase chain reaction (PCR)-sekuensing dari gen 16S rRNA. Tes hemolisis dan metode PCR diterapkan untuk mendeteksi keberadaan gen virulensi toxR, thermostable direct haemolysin (tdh) and tdh-related haemolysin (trh). Metode Kirby Bauer digunakan untuk karakterisasi pola resistansi terhadap ampisilin (AMP), tetrasiklin (TET), kloramfenikol (CHL), siprofloksasin (CIP) dan enrofloksasin (ENR). Uji biokimia dan sekuensing gen PCR-16SrRNA memastikan bahwa 12 isolat adalah V. parahaemolyticus yang selanjutnya diverifikasi dengan amplifikasi gen toxR berukuran 382 bp (100% isolat). Aktivitas alfa hemolisis juga dikonfirmasi dengan amplikon PCR (199 bp) di semua isolat. Seluruh isolat V. parahaemolyticus menunjukkan resistansinya terhadap AMP, 42% resistan TET, tidak ada resistansi yang ditunjukkan pada CIP, ENR dan CHL. Analisis berbasis PCR menghasilkan gen resistan yang terdeteksi dari gen ampC (42% isolat) dan gen tetB (83% isolat). Kata kunci: Antibiotik, udang, resistansi, virulensi, Vibrio parahaemolyticus
Co-Authors Afiff , Usamah Andri Iskandar Cecilia Eny Indriastuti Dian Eka Ramadhani Dian Eka Ramadhani Fachriyan Hasmi Pasaribu Firdausi, Amalia Putri Giri Maruto Darmawangsa Hendriana, Andri Ima Kusumanti Iskandar, Andri Lesmana, Dudi Muarif Muarif Muhamad Erlan Hafid Djunaedi MUNTI YUHANA Nazla Wafi Ratu Mutiara Wali Santoso Santoso Satrio Yudha Wisesa Wida Lesmanawati Wiyoto Wiyoto Yuli Yulianti