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Lactic Acid Bacteria That Produce Antibacterial Compunds on Candied Pakoba Fruit Syzygium luzonense Runtuwene, Kharly; Lawalata, Helen; Posumah, Dany; Satiman, Utari; Moko, Emma
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/srqbqw88

Abstract

Lactic Acid Bacteria (LAB) are a group of Gram-positive, cocci or rod-shaped microorganisms characterized by their catalase-negative phenotype. These bacteria are known to produce various antibacterial compounds, including organic acids, hydrogen peroxide, carbon dioxide, and bacteriocins, which exhibit bacteriostatic or bactericidal properties against pathogenic bacteria. The aim of this study is to isolate LAB that produce antibacterial compounds during the fermentation of Pakoba fruit (Syzygium luzonense) into sweets, with a focus on inhibiting the growth of Escherichia coli and Staphylococcus aureus. This research employs a quantitative descriptive method, involving the isolation of LAB from candied Pakoba fruit samples and subsequent testing of their antibacterial activity against the aforementioned pathogens. The barrier zone formed around the wells is then measured. The data from the study showed that BAL isolation from Pakoba fruit candied was obtained from as many as 10 isolates, and antibacterial activity tests showed that 10 BAL isolates produced antibacterial compounds with inhibitory diameters of 7.6 – 15.3 mm. The isolates with the greatest antibacterial activity are  LAB isolate MP(1)5.2  for E. coli and LAB isolate MP(3)6.3 isolates for S. aureus. Based on the results of identification using the profile matching method, it was shown that the selected LAB isolates, namely MP(1)5.2 and MP(3)6.3, were classified in the genus Lactobacillus with the characteristics of stem cell shape, single cell arrangement, catalase-negative, non-motile, did not form spots, and did not produce gas from glucose.
THE EFFECT OF THE USE OF CHICKEN MANURE ON RED BEANS (Phaseolus vulgaris L.) Goo, Alince; Satiman, Utari; Lawalata, Helen; Tumbel, Ferny Margo; Wantania, Johanna
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/dc0z3470

Abstract

Red beans are a horticultural crop belonging to the annual legume group that grows upright and has a fairly high nutritional content. To obtain maximum growth and yield from red beans, several factors must be considered, including balanced fertilizer application. The factor of providing organic fertilizer from chicken manure with the right dosage is the target of this study. This study aims to determine the effect of red bean growth on the dosage of chicken manure fertilizer. This study was conducted from December 2024 to February 2025 and was carried out on the plantation land of the Department of Science Education, Faculty of Mathematics, Natural Sciences and Earth Sciences, Manado State University. The method used is a quantitative method with a type of experiment in the form of a Randomized Block Design (RAK) with five treatment combinations with each repeated three times. A0 = 0 (control), A1 = 1.00 kg, A2 = 1.50 kg, A3 = 2.00 kg, A4 = 2.50 kg, so there are 15 experimental bed units, and the data obtained are then analyzed using analysis of variance (ANOVA). The results of this study indicate that good plant height growth, number of leaves and stem diameter are found in treatment A1 with a dose of 1.00 kg of chicken manure fertilizer and A2 with a dose of 1.50 kg of chicken manure fertilizer because there is the most maximum growth.
ETHNOBOTANICAL STUDY OF TRADITIONAL MEDICINAL PLANTS AND THEIR UTILIZATION IN PASSI VILLAGE, WEST PASSI DISTRICT, BOLAANG MONGONDOW REGENCY Latip, Irwan; Satiman, Utari; Djarang, Rievo
Indonesian Biodiversity Journal Vol. 6 No. 2 (2025): August 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/k8p6pd73

Abstract

Ethnobotany is the study of the relationship between plants and humans, including the use of plants by local communities. Traditional medicinal plants are plants that are often used by local communities for their health benefits. This study aims to describe the types of medicinal plants used and how they are processed using traditional methods in the village of Passi, West Passi subdistrict, Bolaang Mongondow district. This research is descriptive qualitative with an interview method and descriptive analysis technique. The results of this study show that there are 37 types of medicinal plants used with a total of 23 families found. The methods of processing traditional medicinal plants are: boiling, squeezing, soaking, pounding, and not processing. The most commonly used processing method is boiling, with 24 types of medicinal plants using this method. The parts of the plants that are most commonly used are: stems, fruits, flowers, leaves, roots, gel, sap, rhizomes, and tubers. The most commonly used parts are leaves, rhizomes, and fruits.
Penerapan Model Pembelajaran Problem Based Learning Untuk Meningkatkan Hasil Belajar IPA Siswa Kelas IX C di SMP Negeri 6 Langowan Sukmarayu P Gedoan; Farlen Arlandi Winokan; Utari Satiman; Marthy LS Taulu
PESHUM : Jurnal Pendidikan, Sosial dan Humaniora Vol. 5 No. 4: Juni 2026
Publisher : CV. Ulil Albab Corp

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56799/peshum.v5i4.18141

Abstract

Latar belakang penelitian ini adalah rendahnya hasil belajar siswa pada mata pelajaran IPA. Berdasarkan hasil observasi serta wawancara di SMP Negeri 6 Langowan, pembelajaran di kelas hanya berlangsung satu arah, siswa kurang memperhatikan pelajaran, dan siswa dominan hanya mencatat materi. Penelitian ini bertujuan untuk meningkatkan hasil belajar siswa kelas IX C SMP Negeri 6 Langowan melalui penerapan model pembelajaran PBL khususnya pada materi sistem perkembangbiakan tumbuhan. Penelitian ini menggunakan jenis penelitian tindakan kelas (PTK). Penelitian ini dilaksanakan di kelas IX C SMP Negeri 6 Langowan yang berjumlah 25 siswa. Hasil penelitian siklus I menunjukkan 7 siswa tuntas dengan persentase 28% dan 18 siswa tidak tuntas dengan persentase 72%. Pada pelaksanaan siklus II, 24 siswa tuntas dengan persentase 96% sedangkan 1 siswa tidak tuntas dengan persentase 4%. Kesimpulan penelitian ini bahwa penerapan model pembelajaran problem based learning mampu meningkatkan hasil belajar IPA di kelas IX C SMP Negeri 6 Langowan materi sistem perkembangbiakan tumbuhan.
The Role of Riparian Vegetation in Pollutant Filtration and Sediment Stabilization in Lake Tondano, Indonesia Wiesye Nangoy; Gerry Harindah; Rievo Djarang; Utari Satiman
Quagga: Jurnal Pendidikan dan Biologi Vol 18 No 2 (2026): QUAGGA : Jurnal Pendidikan dan Biologi
Publisher : Program Studi Pendidikan Biologi, Fakultas Keguruan dan Ilmu Pendidikan, Universitas Kuningan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25134/quagga.v18i2.527

Abstract

Riparian vegetation plays a crucial role in maintaining aquatic ecosystem stability by acting as a natural buffer against anthropogenic pollution and erosion. This study analyzed the composition, structure, and ecological functions of riparian vegetation in Lake Tondano, North Sulawesi, Indonesia, with emphasis on pollutant filtration and sediment stabilization. Field surveys were conducted from May to August 2025 at ten riparian stations surrounding Lake Tondano (4,638 ha). Vegetation sampling used a stratified line transect method with fifty plots of varying sizes according to vegetation strata. Quantitative analysis included relative abundance, frequency, dominance, Importance Value Index (IVI), and the Shannon–Wiener diversity index (H’). Ecological functions were assessed through correlations between vegetation structure and reported nutrient and sediment reduction efficiencies from tropical riparian studies. A total of fifteen dominant riparian species were identified, mainly from the families Poaceae and Cyperaceae. Cyperus papyrus (IVI = 21.81%) and Metroxylon sagu (IVI = 29.65%) functioned as key ecological species, while Ficus septica showed the highest IVI (90.83%), indicating strong structural dominance. The Shannon–Wiener diversity index (H’ = 2.65) reflects moderate to high diversity. Riparian vegetation was estimated to reduce nutrient loads by 40–60% and erosion by 30–50%, highlighting its importance for ecosystem-based lake management.
The Effect of Glycerol on the Production of Environmentally Friendly Bioplastics From Cassava Peel (Manihot Esculenta) and Corn Cobs (Zea Mays) Merliana Christmas Turangan; Helen Joan Lawalata; Utari Satiman; Rievo Handry Djarang
Journal of Comprehensive Science Vol. 5 No. 2 (2026): Journal of Comprehensive Science
Publisher : Green Publisher Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59188/jcs.v5i2.4009

Abstract

Conventional plastics made from synthetic polymers have a low biodegradation rate, which contributes greatly to environmental pollution. The use of agricultural waste such as cassava husks (Manihot esculenta) and corn cobs (Zea mays) as bioplastic raw materials is one of the alternatives for the development of environmentally friendly materials. This study aims to determine the effect of variations in the amount of glycerol and the length of decomposition time on the biodegradability of bioplastics made from cassava husks and corn cobs. The study used an experimental method with a Two-Way Factorial Design, covering three concentrations of glycerol (0 mL, 15 mL, and 30 mL) and three decomposition times (3, 6, and 9 days). The results showed that the treatment without glycerol (0 mL) did not form bioplastics so it could not be analyzed. The data was tested using Two Way ANOVA. The results of the biodegradability test showed that the glycerol concentration had a significant effect, and the 30 mL formulation resulted in the highest degradation rate of 91.026% on day 9.