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Journal : Rumphius Pattimura Biological Journal

Nutritional Content of Green Algae Caulerpa racemosa and Brown Algae Sargassum polycystum at Ranowangko II Beach, Kombi District, Minahasa Regency Rorong, Joana Hope; Rampengan, Mercy Maggy Franky; Roring, Verawati Ida Yani; Ogi, Nova Laurin Isye Mourein
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p041-047

Abstract

Ranowangko II beach is famous for its beautiful beaches and is a natural habitat for macroalgae, including Caulerpa racemosa and Sargassum polycystum. Despite its great potential and abundance, information about the nutritional content of both types of algae, especially in the Ranowangko II beach area, is still limited. This study aimed to determine the nutritional content (water content, ash content, protein, lipid, crude fiber, carbohydrate) of green algae Caulerpa racemosa and brown algae Sargassum polycystum in Ranowangko II beach. The type of research used is descriptive quantitative to determine the proximate nutrient content of two types of algae tested by proximate analysis in the laboratory. Based on the study results, there were variations in the nutritional content of the two types of algae tested. Green algae Caulerpa racemosa has a moisture content of 71.29%, ash content of 6.90%, protein of 2.78%, fat of 1.34%, crude fiber of 3.53%, and carbohydrates of 17.69%. Brown algae Sargassum polycystum has a water content of 83.23%, ash content of 1.82%, protein of 9.93%, fat of 1.72%, crude fiber of 11.18%, and carbohydrates of 3.30%. The conclusion obtained after conducting this research is that Caulerpa racemosa (green algae) and Sargassum polycystum (brown algae) have different nutritional content.
Diversity of Echinoderms on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency Letsoin, Imanuel Junisa Adolfina; Rampengan, Mercy Maggy Franky; Roring, Verawati Ida Yani; Lawalata, Helen Joan
RUMPHIUS Vol 6 No 1 (2024): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv6i1p48-55

Abstract

This research is motivated by the lack of studies and absence of research data on the diversity of Echinoderms on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency. The aim of this study is to determine the diversity of the phylum Echinodermata found on the Beach of Ranowangko II Village, Kombi District, Minahasa Regency. Sampling in this research used the quadrat transect method with 3 transect lines, each line being 100 m long, and 10 quadrats, each measuring 1 x 1 m². The results of the study found 4 classes and 5 species including the class Asteroidea (Protoreaster nodosus), class Ophiuroidea (Ophiocoma scolopendrina), class Echinoidea (Tripneustes gratilla), class Holothuroidea (Holothuria atra & Holothuria vagabunda), and there were 21 individuals, and the dominant species is Holothuria atra from the Holothuroidea class of which there are 11 individuals, indicating that the diversity index (H’) of the phylum Echinodermata on the location is moderate with a value of 1.204. These findings offer initial insights into the condition of the local ecosystem, classified as moderate, and may serve as a foundational reference for long-term ecological monitoring.
Effectiveness of Sea Cucumber (Holothuria atra) Extract Ointment from the Talaud Islands on Burn Wound Healing in Mice (Mus musculus) Mumu, Trivena Vannesa; Roring, Verawati Ida Yani; Manampiring, Nonny
RUMPHIUS Vol 8 No 1 (2026): RUMPHIUS Pattimura Biological Journal
Publisher : Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/rumphiusv8i1p061-070

Abstract

Sea cucumber (Holothuria atra) has been traditionally used for wound treatment; however, scientific evidence supporting its effectiveness in burn wound healing remains limited. This study presents a novel investigation of the wound healing potential of H. atra extract sourced specifically from the Talaud Islands and formulated into multiple topical ointment concentrations for second-degree burn treatment. The study aimed to evaluate the effectiveness of H. atra extract ointment in accelerating second degree burn wound healing in mice. The extract was obtained by maceration using 95% ethanol and formulated into ointments at concentrations of 10%, 15%, and 20%. Twenty-five male mice were randomly assigned to five groups: positive control (1% silver sulfadiazine), negative control, and three treatment groups receiving H. atra extract ointments at different concentrations. Parameters observed included wound diameter reduction, physical characteristics of the ointment, phytochemical screening, and pain-related behavioral responses. The results demonstrated that the 15% H. atra extract ointment produced the highest wound healing effectiveness, with a wound contraction rate of 96% on day 14, followed by the 10% (84%) and 20% (75%) formulations. Phytochemical analysis revealed the presence of flavonoids, saponins, steroids, and alkaloids, which are associated with anti-inflammatory, antimicrobial, and tissue regenerative activities. The ointment formulations met homogeneity and organoleptic requirements, although their spreadability values were below the recommended standard. These findings highlight the novelty of identifying an optimal concentration (15%) of H. atra extract ointment that provides superior burn wound healing activity compared with other tested concentrations. In conclusion, the 15% H. atra extract ointment shows potential as an alternative topical therapy for second-degree burn wounds.