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Isolasi dan Identifikasi Bakteri yang Terdapat pada Saluran Pencernaan Ikan Gurami (Osphronemus gouramy) dengan Pemberian Pakan Komersil yang Berbeda [Isolation And Identification Of Bacterial In The Digestive Organ Of Gurami Fish (Osphronemus gouramy) With Different Commercial Feed] Sri Subekti; Franch Dalahi; Agustono Agustono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 6 No. 1 (2014): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v6i1.11385

Abstract Based on the food habits, gurami is omnivorous fish that feed herbivorous carp tend consists of natural food and artificial feed (pellets). The feed is needed to support the growth and survival of Gurami. If feed is given in accordance with the eating habits of Gurami and contain high nutritional Gurami can then growth accelerated faster. Some types of bacteria found in the digestive tract of animals has an important role in order to improve feed utilization, fish health, and environmental quality improvement and microorganisms. In addition, some bacterial flora in the gastrointestinal tract plays an important role and produce several types of enzymes in the digestive tract may contribute to the metabolism of the host. The purpose of this study was to determine the bacteria in the digestive tract of Gurami (Osphronemus gouramy) were given three different types of commercial feed. This study begins with a sampling of Gurami (Oshpronemus gouramy) of maintenance in the Faculty of Fisheries and Marine at Airlangga University in Surabaya and then do stage isolation and identification of bacteria in laboratory bacteria and Microbiology, Faculty of Veterinary Medicine, Airlangga University Surabaya. This study was conducted in July 2013. This research is a descriptive study, the method of solving the problem by describing the subject matter into a form that is easy to understand and based on facts. Results of research on gurami fish that given by three different types of commercial feed, there are two types of bacteria that dominate in the digestive tract of carp, the Pseudomonas sp. and Serratia sp. Both types of bacteria has the potential to help the digestive system of gurami fish. The result of this research are need for further research using the bacterium Pseudomonas sp. and Serratia sp. as probiotics.

Pemberian Enzim Lignosellulosa dalam Pakan Buatan Terhadap Pertumbuhan dan Tingkat Kelulushidupan Benih Ikan Gurami (Osphronemus gouramy Lac.) [The Granting Of An Enzyme Lignosellulosa in Feed Artificial On The Growth and The Survival Rate Seed Gourami (Osphronemus gouramy Lac.) ] Agustono Agustono; Muhammad Yusuf Akbar; Rahayu Kusdarwati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11401

Abstract Gurami having economic values of. Have abundance, namely able in waters with its oxygen relatively low. Belong to the species of carnivorous herbs herbivora. Unprofitableness is eat feed derived from herbs with content nutrition relatively low compared to animals, so it impact on its growth slow, easly diseases, easly stress, hard eat and SR low. The purpose of this research isto know the addition of enzyme lignosellulase in artificialfeedincreased growth and increased survival rate seed gourami. Method research used is experimental with delightful random complete (RAL) with five treatment and four deuterenomy. The treatment used were : control (A), enzyme 550ml (B), enzyme 600ml (C), enzyme 650ml (D), and enzyme 750ml(E). The main parameters measured were growth rate, and survival rate. The supporting parameters observedwas water quality. Data analysis used analysis of variance (ANOVA) to know the effect of the treatments.To know the difference among treatments used Duncan's Multiple Range Test (DMRT). The result showed that the provision of enzymes give a real power (p<0.01) against growth rate daily and growth long absolute gourami (Osphronemus gouramy) To survival rate and feed conversion ratio gourami give impact which is not dissimilar real. Growth best on treatment E (0.0214), then successive followed by treatment D (0.0174), C (0.015), B (0.0142) and A (0.0128). Conversion ratio feed on all treatment e 10,415, that is, e then successive followed by treatment d (12,915), c (14,3975), b (15,6375) and a (17,5325). Survival rate obtained 100%. Water quality media maintenance gourami is temperature 26ºC - 29ºC, pH 7 – 8, oxygen dissolved 3.5 – 5 mg/l and ammonia 0.004 – 0.005 mg/l.

Pengukuran Kecernaan Lemak Kasar, Bahan Organik dan Energi pada Pakan Ikan Nila (Oreochromis niloticus) dengan Menggunakan Teknik Pembedahan [Measurements Of Extract Ether Digestibility, Organic Matter and Energy Feed In Tilapia (Oreochromis niloticus) Using Surgery Techniques] Agustono Agustono; Rahardian Wicaksono; Widya Paramita Lokapirnasari
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11409

Abstract Tilapia is a freshwater fish species are widely cultivated in Indonesia. Tilapia production continues to grow every year, the average increase in the number of experienced production tilapia reaches 22.24% in the 2010-2011 range. Tilapia production in 2011 reached 567.449 tons and was second aquaculture production by major commodity after seaweed. This study aimed to determining differences in extract ether digestibility, organic matter, and energy on feed derived from different factories in tilapia (Oreochromis niloticus) using surgery techniques. The research design is using completely randomized design. The variables were measured extract ether digestibility, organic matter, and energy in tilapia feed. Analysis of the data processed using Analysis of Variance (ANOVA) to determine differences in treatment given. If there is a difference then followed by Duncan's Multiple Range Test with a significance level of 5%. Based on the findings that there were no significant differences (p>0.05) in extract ether , organic matter, and energy digestibility of the feed value of tilapia. The results stated that the feed treatments A, B and C efficiently digested by tilapia.

Substitusi Tepung Bungkil Kedelai dengan Fermentasi Daun Lamtoro (Leucaena glauca) Terhadap Pertumbuhan dan Efisiensi Pakan Ikan Nila (Oreochromis niloticus) [Subtitution Of Soybean Meal With Fermented Lamtoro (Leucaena glauca) On Growth And Feed Efficiency Of Tilapia Fish (Oreochromis niloticus) ] Agustono Agustono; Novi Krishatno Putera; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 2 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i2.11410

Abstract Tilapia fish (Oreochromis niloticus) was known as an important commodity in the world's freshwater. Tilapia fish (O. niloticus) was a omnivores species of fish, which means that can eat plants and animals. Tilapia fish easy to breed, fast growing, relatively has large body size, disease resistance, easy to adapt to the environment, the price was relatively cheap and has a high nutritional value as a source of animal protein. One of the obstacles faced in the feed manufacturing was an expensive raw material availability, therefore, necessary to find alternative feed ingredients that were cheap, quality and be available at all the times. The purpose of this study was to determine the effect of the use lamtoro were fermented with probiotic on growth and feed efficiency of tilapia fish. The research was conducted at the Faculty of Fisheries and Marine University. This study was conducted for 42 days from 25 March - 5 May 2013. The research design used was a completely randomized design (CRD). Materials used in this study was tilapia fish aged 1 month, the body length ranges between 3-5 cm and body weight ranged from 1,3-1,5 gram as 100 individuals. Form of feed pellets substituted with lamtoro fermented dose A. 0% (control), B. 10%, C. 20%, D. 30%, E. 40% with 4 replications. The main parameters observed in this research were the growth rate and feed efficiency. Support parameters was the quality of water. Based on the results of research about substitution of soybean meal with fermented lamtoro in the feed rations were no significantly different effect on the rate of growth and feed efficiency (p> 0.05) due to dissolved oxygen content does not qualify as well as, higher quality feed than feed quality substituted.

Substitusi Bungkil Kedelai dengan Ddgs (Distillers Dried Grains and Solubles) terhadap Kandungan Lemak Kasar dan Energi Daging Ikan Nila Merah (Oreochromis niloticus) [Substitution Of Soybean Meal With Ddgs (Distillers Dried Grains And Solubles) Content Of Crude Fat And Energy Meat Red Tilapia (Oreochromis niloticus) ] Agustono Agustono; Sathiul Inayah; M. Anam Al Arif
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11421

Abstract Red tilapia (O. niloticus) is a food fish including efforts to increase the commodity into aquaculture production in 2014 amounted to 353%. To support efforts to increase production, so it needed one attempt that the cultivation of tilapia. One effort to reduce the cost of feed needed alternative feed ingredients were good quality. One of the alternatives that are cheap and high nutritional value is DDGS. DDGS is a byproduct from dry milling ethanol industry. Although the by-product of milling corn and the price is cheaper than soybean meal but the nutritional content is similar to soybean meal. From the results of the proximate analysis of DDGS contains 28% protein, 17% fat, 10% crude fiber, 27% and energy BETN 3150 kcal / kg. The results showed that the use of DDGS as a substitute for soybean meal until 80% did not cause significant differences (p> 0.05) on crude fat and energy content of meat red tilapia (O.niloticus).

Pengaruh Medium yang Tercemar Organoklorin (Endosulfan) terhadap Kandungan Agar dan Morfologi Thallus Gracilaria verrucosa [Effect Of Organochlorines (Endosulfan) Contaminated Medium On Content Of Gelatin and Thallus Morphology Gracilaria verrucosa] Moch. Amin Alamsjah; Handini Fidya Riswanti; Agustono Agustono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11425

Abstract Gracilaria verrucosa seaweed is one of the high economic value because its potential as raw with the good content of more agarose than agaropektin so that it can produce gelatin to form strong and solid gelatin. The content range is from 12-48% depending on the class species Rhodophyceaea. Organochlorine residues (Endosulfan) come down to sea by the use of Organochlorine continuously, finally settles on the ground and carried by the flow of rain. Organochlorine residues (Endosulfan) excess in aquatic may influence biota so that can degrade and change the content that Thallus morphology on Gracilaria verrucosa. This research aims to determine the content of that reduction and morphological changes of Gracilaria verrucosa thallus on organochlorine contaminated medium (Endosulfan). This research method s experimental, while the design of the study is a Rancangan Acak Lengkap (RAL) with 5 treatments and 4 replications. The main parameters in this research that content of gelatin and thallus morphology Gracilaria verrucosa, while supporting parameters in this study consisted of: water quality medium. The results showed that the organochlorine contaminated medium (Endosulfan) significantly influenced (p <0.05) of the gelatin content. Ingredients that are highest in treatment A (41.2%). Content that is second highest on treatments B (35.55%). Ingredients that are the third highest in treatment C (22.02%), followed by treatment D (16.72%) and treatment E (13%) showed the lowest gelatin. Each treatment showed significantly different between treatments. Thallus morphology Observations show differences in the thickness of the thallus epidermis such as the dose of pollutant. The greater the dose of organochlorines (Endosulfan) given the Thallus epidermis G. verrucosa diminution.

Fermentasi Ampas Kelapa menggunakan Trichoderma viride, Bacillus subtilis, dan EM4 terhadap Kandungan Protein Kasar dan Serat Kasar sebagai Bahan Pakan Alternatif Ikan [Fermentation Of Coconut Dregs Using Trichoderma viride, Bacillus subtilis, And EM4 Against Crude Protein And Crude Fiber As An Alternative Feed Ingredients For Fish] Yudi Cahyoko; Hiprita Putri Karlina; Agustono Agustono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11429

Abstract Feed plays an important role in fish farming. Feed requirements achieve 60-70% of the cost of fish farming operations. Availability of feed with quality and sufficient quantity is aimed in increasing fish production. A very high feed prices due to artificial feed ingredients used are expensive and required in large quantities. Therefore, it is necessary to find alternatives to fish directly or indirectly obtaining appropiate and adequate nutrition needs to grow. The raw material content used for feed is coconut dregs (Cocos nucifera). The availability of the coconut dregs is abundant and potensial for fish feed ingredients. The coconut dregs is also one type or plantation waste which still has potential to be processed into the manufacture of fish feed. The coconut dregs flour can be processed by fermentation to improve quality. This study was aimed to determine the increase in crude protein content and a decrease in crude fiber content of coconut dregs fermented with Trichoderma viride; Bacillus subtilis and EM4. The research method was an experiment with Completely Randomized Design (CRD). The treatments used without the addition of microbes (P0), Bacillus subtilis 6% (P1), Trichoderma viride 6% (P2), and EM4 6% (P3) with 5 repeatitions each. The parameters observed were the content of crude protein and crude fiber after 7 days of fermentation. Data analysis used Analysis of Variance (ANOVA) and to determine the best treatments using Duncan Multiple Distance Test with 5% confidence interval. The results indicated that coconut dregs fermented with Bacillus subtilis (P1), Trichoderma viride (P2), and EM4 (P3) produced the difference of crude protein and fiber. The best treatment on the increase in crude protein content was Bacillus subtilis (P1) of 7.5564%. The best treatment on the decrease in crude fiber content was EM4 (P3) of 22.3967%. However, the results of the flour fermented coconut dregs can not be used as an alternative feed material because the fermented coconut dregs is not qualified for fish feed. This is due to a lack of crude protein and high crude fiber content.

Pengaruh Pemberian Pakan Beryodium terhadap Pertumbuhan dan Kandungan Yodium Benih Ikan Mas (Cyprinus carpio) [Effect Of Iodized Feeding On The Growth And Content Of Iodine In Carpfish Seed (Cyprinus carpio) ] Agustono Agustono; Lia Enggar C. W; Mirni Lamid
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11431

Abstract Carpfish culure have a quiet effort to profit and developed by people, because it have characteristic like adapted with the environtment, have rapid growth and resistance of disease. Carpfish cultivation is often cultivated in mountainous area far from sources of iodine (Cholik etc, 2002 ; Susanto, 1997). This causes iodine contain is very low, carp fish has only 1.7 mg/100g iodine contain. That amount is not sufficient people nutrition needs. Purpose of this study was to increase the iodine contain and growth. Experiment have been conducted in April until May 2011, for 1 month in Aquaculture Development Laboratory Hang Tuah University, Surabaya. Data retrieval was done by observation, direct participation and documentation thechnique. The research method is an experiment method with a completely randomized desigen consisted of 5 treatment and 4 replication, at a dose of 0 mg / kg of feed, 20 mg / kg of feed, 40 mg / kg of feed, 60 mg / kg feed and 80 mg / kg of feed. Each treatment used 15 carp seed (Cyprinus carpio) measuring 5-8 cm is maintained for 30 days. The parameter used include the primary parameter and parameter support. The main parameter of the growth and content the iodine, while supporting the water quality parameter. Result of this study were analized by using analysis of various with an error rate 5% later, followed by Duncan multiple rate test. Result of this study indicate that feeding iodized can increased the growth in wight and iodine content of carp.

Pengaruh Penambahan Atraktan yang Berbeda dalam Pakan Pasta Terhadap Retensi Protein, Lemak dan Energi Benih Ikan Sidat (Anguilla bicolor) Stadia Elver [ Effects Of Addition Different Attractants In Pasta Feed Against Retention Of Protein, Fat and Energy Eel Fish Seed (Anguilla bicolor) Stadia Elver] Muhammad Arief; Suryo Yudiarto; Agustono Agustono
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11564

Abstract Indonesia has a great potential for the development of eel fish (Anguilla bicolor) farming. Availability of seeds is not enough to make the farmers are interested to cultivate eel fish because fish growth is long enough. It can be addressed through accelerated consumption of feed with the proper administration of feed attractants that can spur growth. This study aimed to determine the effect of the addition of different attractants in pasta feed on the retention of protein, fat and energy of eel fish seed (Anguilla bicolor) stadia Elver. The experiment was conducted at the Faculty of Fisheries and Marine Resources, University of Airlangga. Proximate analysis of feed ingredients, fish proximate analysis of the beginning and end of the study conducted in Unit Inspection Service laboratory Consulting and Training Faculty of Veterinary Medicine, University of Airlangga. Research using a pasta feed plus attractants shrimp oil (A), eel oil (B), milkfish oil (C), squid oil (D) and without attractant (E) with the main parameters, namely retention of protein, fat and energy and support parameters that water quality, including temperature, solubility of oxygen, pH and ammonia. Analysis of data using Variety Analysis (ANOVA) and to determine the differences between treatments performed tests Distance Regression Duncan. The results showed that the addition of different attractant in feed pasta provide the best protein retention in treatment E (12.83%) and then a row followed by treatment C (11.98%), D (11.45%), B (10 , 26%) and A (7.78%). Best energy retention in treatment E (10.77%), then a row followed by treatment D (9.59%), C (9.47%), B (8.65%) and A (8.27% ). Best fat retention in treatment D (3.76%) and lowest in the C treatment (2.12%). Water quality maintenance media eel fish are temperature 28 º -28.5 º C, pH 7-8, dissolved oxygen 4-8 mg / L and ammonia from 1.5 to 2 mg / L.

Kandungan Bahan Kering, Serat Kasar dan Protein Kasar pada Daun Lamtoro (Leucaena glauca) yang Difermentasi dengan Probiotik sebagai Bahan Pakan Ikan [The Content Of Dry Matter, Crude Fiber and Protein from Fermentation Of Leucaena glauca Leaf Using Probiotic As Fish Feed Material] Agustono Agustono; Devy Rahmawati Putri; Sri Subekti
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 2 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i2.11567

Abstract Feed is the element that really support the activities of aquaculture, there for the feed that is available must be adequate and meet the needs of the fish. Efforts to reduce feed costs, some farmers use alternative feed ingredients as a substitute for feed ingredients. Lamtoro leaves in the form of a mixture of flour can be used as fish feed in pellet form. This research been used method with used Completely Randomized Design (CRD) with five treatments and four replications. The treatments used were control (P0), the provision of Probiotics 2% (P1), giving Probiotic 4% (P2), Probiotics administration 6% (P3) and the provision of Probiotics 8% (P4) with each repeated 4 times. Parameters observed were protein content of crude fiber and crude after fermentation for 7 days. These results indicate that administration of probiotics 8% gave significant effect toward crude fiber content in the leaves are fermented and lamtoro which been given Probiotics 6% gave a highly significant effect of the increase in crude protein content in the fermented leaves lamtoro.

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